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The map label for this gene is ilvB

Identifier: 226224582

GI number: 226224582

Start: 2044943

End: 2046664

Strand: Direct

Name: ilvB

Synonym: Lm4b_01996

Alternate gene names: 226224582

Gene position: 2044943-2046664 (Clockwise)

Preceding gene: 226224581

Following gene: 226224583

Centisome position: 70.21

GC content: 43.03

Gene sequence:

>1722_bases
GTGATTGATACGACGAAGACAAATGAAAAAGCGACAAAAAAGCAAAATAAAAGTGGTGCGGAACTTTTAATTGACTCCTT
GAAAAAGCAACAAGTCGAGATGATTTTTGGCTATCCGGGTGGTGCGGTTCTCCCTCTCTACGATGCTTTTTACGACTGTG
ATATTCCGCATATTCTAACAAGACACGAGCAAGGAGCGATTCATGCTGCGGAAGGTTATGCACGTGTTACTGGGAAACCC
GGTGTGGTAGTTGTAACAAGTGGACCTGGGGCGACAAATGTATTAACTGGTATTGCGGATGCAATGAGTGATTCGATTCC
ATTAGTGATTTTCACCGGACAAGTTCATACGCCTGGTATCGGAAAAGATGCCTTCCAAGAAGCAGATATGATTGGGCTAA
CCATTCCAATTACTAAATATAATTACCAAGTGCGTGATGTTCGTGACTTACCAAAAATTGTCAATGAAGCTTTTCATATT
GCAAGCACTGGTCGTAAAGGTCCTGTTGTCGTGGATATTCCAAAAGACATGGGGATTATCCAAACAGAGTCTGTACGACC
AGATACGATTGATCTGCCGGGTTATCAACCGACTTATTCACCGAACCCACTTCAACTGGAAAAACTAATGCAATCTCTTT
CTGCCGCAAGCAAGCCACTAATACTTGCCGGAGCTGGCGTCAATCATGCGAGAGCTACAGCTGAATTACTAGAATTCGCT
GAACGCTATCAAATTCCAATCGTGAATACGCTGCTTGGTCTAGGAAGTTTCCCGCAAAGTCATGATTTATTCCTTGGTAT
GGGCGGCATGCACGGTTCATACGCGGCAAACATGGCACTTACAGATTGCGATTTGCTCATTAATTTTGGTTCCCGCTTCG
ACGACAGGCTTGCCAGTGCGCCAAAAGAATTTGCCACAAAAGCAACGATTGCACATATCGATATTGATCCTGCTGAAATT
GGCAAAATTATCGAAACACAAATTCCGATTGTAGCTGATATTAACGAAACACTTACACAACTACTACAAATGGAACTTCC
GGTTCATCCGGATACATCTCATTGGTACAAATTAAATATGACCCGAAAAAACCGTCATCCTTTTAATTTTGATAGAACGA
AAAAAGCAGAAATTAAACCACAGCGTGTGATTGAGCTAATTGGTGAAATTACGCAAGGTGAAGCGCTTGTTTCTACCGAT
GTTGGCCAGCATCAAATGTGGGCGGCACAGTTTTATCCATTCCAATTTGATCATCAAATTATTACGAGTGGCGGGCTTGG
CACAATGGGCTTTGGCTTTCCAGCAGCTGTTGGTGCGCAACTTGCTTTCCCAGATAAAACGGTAGTCGCCATTGTTGGGG
ATGGTGGTTTCCAAATGACTAATCAAGAACTGGCGATTTTAAATGATTACCAAATCAATGTGAAAACGGTCATTATCAAT
AACGGTTCGCTCGGCATGGTTCGTCAGTGGCAAGAAAAATTCCACGGCGAAAGATATTCTCATTCGATTTTCACGAGCCA
ACCAGATTTTGTTAAACTATCCGAAGCTTACGGTGTCAAAGGGGTTCGCCTGACAAATCCTGAAACGCTAGAAAAAGATC
TAAGAAAAGCCTTCGCTCACCCTGGACCGATTGTCATTGACGTTCATGTAGCCAAAGATGAGCTAGTACTTCCAATGGTT
CCAAGCGGCAAACCAAATCACCAAATGGAAGGAGTGGAATAA

Upstream 100 bases:

>100_bases
GTTACCTTGCAAGATATACTGCGCTTGTAACTAGCGCCCACACTGGTGGCATTTTGCAAATTCCAGAAGATTTAATCGAC
TAAAAAATGAGGTGATAAGC

Downstream 100 bases:

>100_bases
ATGCGCCGAATCATTACCGCTACAGTAAACAACTCTTCTGGCGTGTTAAATCGTATCACTGGGGTTATCTCCAGACGCCA
ATACAATATTGATAGCATCT

Product: acetolactate synthase (acetohydroxy-acid synthase) (large subunit)

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MIDTTKTNEKATKKQNKSGAELLIDSLKKQQVEMIFGYPGGAVLPLYDAFYDCDIPHILTRHEQGAIHAAEGYARVTGKP
GVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGIGKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHI
ASTGRKGPVVVDIPKDMGIIQTESVRPDTIDLPGYQPTYSPNPLQLEKLMQSLSAASKPLILAGAGVNHARATAELLEFA
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASAPKEFATKATIAHIDIDPAEI
GKIIETQIPIVADINETLTQLLQMELPVHPDTSHWYKLNMTRKNRHPFNFDRTKKAEIKPQRVIELIGEITQGEALVSTD
VGQHQMWAAQFYPFQFDHQIITSGGLGTMGFGFPAAVGAQLAFPDKTVVAIVGDGGFQMTNQELAILNDYQINVKTVIIN
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAHPGPIVIDVHVAKDELVLPMV
PSGKPNHQMEGVE

Sequences:

>Translated_573_residues
MIDTTKTNEKATKKQNKSGAELLIDSLKKQQVEMIFGYPGGAVLPLYDAFYDCDIPHILTRHEQGAIHAAEGYARVTGKP
GVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGIGKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHI
ASTGRKGPVVVDIPKDMGIIQTESVRPDTIDLPGYQPTYSPNPLQLEKLMQSLSAASKPLILAGAGVNHARATAELLEFA
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASAPKEFATKATIAHIDIDPAEI
GKIIETQIPIVADINETLTQLLQMELPVHPDTSHWYKLNMTRKNRHPFNFDRTKKAEIKPQRVIELIGEITQGEALVSTD
VGQHQMWAAQFYPFQFDHQIITSGGLGTMGFGFPAAVGAQLAFPDKTVVAIVGDGGFQMTNQELAILNDYQINVKTVIIN
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAHPGPIVIDVHVAKDELVLPMV
PSGKPNHQMEGVE
>Mature_573_residues
MIDTTKTNEKATKKQNKSGAELLIDSLKKQQVEMIFGYPGGAVLPLYDAFYDCDIPHILTRHEQGAIHAAEGYARVTGKP
GVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGIGKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHI
ASTGRKGPVVVDIPKDMGIIQTESVRPDTIDLPGYQPTYSPNPLQLEKLMQSLSAASKPLILAGAGVNHARATAELLEFA
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASAPKEFATKATIAHIDIDPAEI
GKIIETQIPIVADINETLTQLLQMELPVHPDTSHWYKLNMTRKNRHPFNFDRTKKAEIKPQRVIELIGEITQGEALVSTD
VGQHQMWAAQFYPFQFDHQIITSGGLGTMGFGFPAAVGAQLAFPDKTVVAIVGDGGFQMTNQELAILNDYQINVKTVIIN
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAHPGPIVIDVHVAKDELVLPMV
PSGKPNHQMEGVE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=567, Percent_Identity=24.8677248677249, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI21361361, Length=594, Percent_Identity=24.2424242424242, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=44.6043165467626, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=40.4634581105169, Blast_Score=424, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=544, Percent_Identity=34.0073529411765, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=29.1208791208791, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1788716, Length=546, Percent_Identity=23.8095238095238, Blast_Score=136, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=27.4165202108963, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=27.4165202108963, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=573, Percent_Identity=24.4328097731239, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=43.8380281690141, Blast_Score=460, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320816, Length=497, Percent_Identity=24.1448692152917, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=484, Percent_Identity=23.5537190082645, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=483, Percent_Identity=23.3954451345756, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=487, Percent_Identity=22.5872689938398, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=549, Percent_Identity=26.2295081967213, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62695; Mature: 62695

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTTKTNEKATKKQNKSGAELLIDSLKKQQVEMIFGYPGGAVLPLYDAFYDCDIPHILT
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHCEEEEEEECCCCCEEEHHHHHHCCCCCHHHH
RHEQGAIHAAEGYARVTGKPGVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGI
CCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHIASTGRKGPVVVDIPKDMGII
CHHHHHCCCEEEEEEEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEE
QTESVRPDTIDLPGYQPTYSPNPLQLEKLMQSLSAASKPLILAGAGVNHARATAELLEFA
EECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASA
HHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHEEECCCCHHHHHHCC
PKEFATKATIAHIDIDPAEIGKIIETQIPIVADINETLTQLLQMELPVHPDTSHWYKLNM
CHHHHCCEEEEEEECCHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEE
TRKNRHPFNFDRTKKAEIKPQRVIELIGEITQGEALVSTDVGQHQMWAAQFYPFQFDHQI
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCEEECCEE
ITSGGLGTMGFGFPAAVGAQLAFPDKTVVAIVGDGGFQMTNQELAILNDYQINVKTVIIN
EECCCCCCCCCCCHHHHCCEEECCCCEEEEEEECCCCEECCCCEEEEECEEEEEEEEEEE
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAH
CCCCCHHHHHHHHHCCCCCCCCEECCCCCEEEEHHHCCCCCEEECCHHHHHHHHHHHHCC
PGPIVIDVHVAKDELVLPMVPSGKPNHQMEGVE
CCCEEEEEEECCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MIDTTKTNEKATKKQNKSGAELLIDSLKKQQVEMIFGYPGGAVLPLYDAFYDCDIPHILT
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHCEEEEEEECCCCCEEEHHHHHHCCCCCHHHH
RHEQGAIHAAEGYARVTGKPGVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGI
CCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHIASTGRKGPVVVDIPKDMGII
CHHHHHCCCEEEEEEEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEE
QTESVRPDTIDLPGYQPTYSPNPLQLEKLMQSLSAASKPLILAGAGVNHARATAELLEFA
EECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASA
HHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHEEECCCCHHHHHHCC
PKEFATKATIAHIDIDPAEIGKIIETQIPIVADINETLTQLLQMELPVHPDTSHWYKLNM
CHHHHCCEEEEEEECCHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEE
TRKNRHPFNFDRTKKAEIKPQRVIELIGEITQGEALVSTDVGQHQMWAAQFYPFQFDHQI
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCEEECCEE
ITSGGLGTMGFGFPAAVGAQLAFPDKTVVAIVGDGGFQMTNQELAILNDYQINVKTVIIN
EECCCCCCCCCCCHHHHCCEEECCCCEEEEEEECCCCEECCCCEEEEECEEEEEEEEEEE
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAH
CCCCCHHHHHHHHHCCCCCCCCEECCCCCEEEEHHHCCCCCEEECCHHHHHHHHHHHHCC
PGPIVIDVHVAKDELVLPMVPSGKPNHQMEGVE
CCCEEEEEEECCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]