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The map label for this gene is ilvD

Identifier: 226224581

GI number: 226224581

Start: 2043231

End: 2044925

Strand: Direct

Name: ilvD

Synonym: Lm4b_01995

Alternate gene names: 226224581

Gene position: 2043231-2044925 (Clockwise)

Preceding gene: 226224578

Following gene: 226224582

Centisome position: 70.15

GC content: 43.3

Gene sequence:

>1695_bases
ATGCGTAGTGACAAAATAAAAAAAGGTGTCGAACAAGCTCCAGCAAGAAGTTTGCTGCATGCTACAGGTCAAATTAAAAG
TCCAGGTGATATGGATAAACCATTTATCGCTATTTGTAACTCTTACATTGATATCGTTCCTGGTCATGTTCATTTGCGAG
AATTAGCGGATGTGGCTAAAGAAGCTATTAGAGAAGCTGGCGGTATCCCATTTGAATTTAATACGATTGGTGTAGATGAC
GGCATTGCGATGGGACATATCGGTATGCGCTATTCCCTTCCCTCTCGTGAAGTTATCGCGGATGCAGCGGAAACGGTAAT
CAATGCGCACTGGTTTGATGGGGTTTTCTACATCCCAAACTGTGACAAAATCACGCCTGGAATGCTTCTCGCTTCTGTTC
GTACAAATGTACCTGCTATCTTCTGTTCTGGTGGCCCAATGAAAGCTGGATTATCTGCACACGGAAAAGCACTCACTCTC
TCTTCTGTTTTTGAAGCGGTTGGCGCATTTAAAGATGGCAGCATGTCGCAGGAAGATTTTCTTGATATGGAAGCTAATGC
GTGTCCGACTTGCGGATCTTGCGCAGGAATGTTTACAGCAAACTCAATGAATTGTTTGATGGAAATCCTAGGTATGGCTG
TTCCGGGAAATGGTACAACACTTGCTGTTTCTGACGCACGCCGTGATCTTATTAGAGAATCTGCTTTTCATTTAATGGAT
TTAGTTAAAAAAGATATTCGTCCTCGTGACATTATTACAAAAGATGCGATTGACGATGCTTTTGCGCTAGATATGGCAAT
GGGTGGTTCGACAAATACCGTATTACATACACTTGCACTTGCAAACGAAGCTGGTATTGAAGATTATGATTTGGAACGTA
TCAATGATATTGCTAAACGTGTTCCCTACCTTTCCAAAATTGCACCTTCCTCTTCTTACTCAATGCATGATGTTCACGAA
GCTGGCGGTGTTTCAGCCATTGTTAAAGAACTGGTTGATCTAGGTGGCGCAATACATCCAGACAGAATCACTGTTACTGG
TAAAACGATTCGCGAAAACGTAGCTGATGCAAAAATTAATAATACCGATGTTATTCATCCAAAAGAAAATCCGTACAGCC
CAGTTGGCGGACTTTCGATGTTGTTTGGTAACATCGCACCAAAAGGAGCTGCTATCAAAGTTGGTGGCGTAGATCCTTCC
GTACAAGTTTTCAAAGGAGAAGCGATTTGCTTTAGTTCTCATGATGAGGCGGTGGAAGCAATTGATAACCATACCGTCCG
CGAGGGGCATGTCGTGGTCATTCGCTACGAAGGTCCTAAAGGCGGTCCGGGAATGCCTGAAATGCTTGCGCCAACATCTA
GTATTGTTGGTCGAGGTTTAGGAAAAGATGTTGCTTTGATTACAGATGGTCGCTTTTCTGGGGCTACTCGTGGTATCGCA
GTTGGCCACATTTCTCCAGAAGCTGCAGCTGGTGGTCCTATCGCCCTTGTTCATGACGGCGATATCATCACGATTGATTT
GCCAAACCGGACGTTAAACGTGGATGTTTCTGATGAGGTTTTGGAAGAACGAAGAAAAGAATTACCGAAATTTAAAGCTA
AAGTAAAAACTGGTTACCTTGCAAGATATACTGCGCTTGTAACTAGCGCCCACACTGGTGGCATTTTGCAAATTCCAGAA
GATTTAATCGACTAA

Upstream 100 bases:

>100_bases
AGCAGCTACTTAATCATATATATTAATGCCACGCTATTTAGTGAATTCTAAAAATTCAGTGTCGGCAAATAATTCTTAAT
TAGAAATGGGGTAAAGTCAT

Downstream 100 bases:

>100_bases
AAAATGAGGTGATAAGCGTGATTGATACGACGAAGACAAATGAAAAAGCGACAAAAAAGCAAAATAAAAGTGGTGCGGAA
CTTTTAATTGACTCCTTGAA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MRSDKIKKGVEQAPARSLLHATGQIKSPGDMDKPFIAICNSYIDIVPGHVHLRELADVAKEAIREAGGIPFEFNTIGVDD
GIAMGHIGMRYSLPSREVIADAAETVINAHWFDGVFYIPNCDKITPGMLLASVRTNVPAIFCSGGPMKAGLSAHGKALTL
SSVFEAVGAFKDGSMSQEDFLDMEANACPTCGSCAGMFTANSMNCLMEILGMAVPGNGTTLAVSDARRDLIRESAFHLMD
LVKKDIRPRDIITKDAIDDAFALDMAMGGSTNTVLHTLALANEAGIEDYDLERINDIAKRVPYLSKIAPSSSYSMHDVHE
AGGVSAIVKELVDLGGAIHPDRITVTGKTIRENVADAKINNTDVIHPKENPYSPVGGLSMLFGNIAPKGAAIKVGGVDPS
VQVFKGEAICFSSHDEAVEAIDNHTVREGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIA
VGHISPEAAAGGPIALVHDGDIITIDLPNRTLNVDVSDEVLEERRKELPKFKAKVKTGYLARYTALVTSAHTGGILQIPE
DLID

Sequences:

>Translated_564_residues
MRSDKIKKGVEQAPARSLLHATGQIKSPGDMDKPFIAICNSYIDIVPGHVHLRELADVAKEAIREAGGIPFEFNTIGVDD
GIAMGHIGMRYSLPSREVIADAAETVINAHWFDGVFYIPNCDKITPGMLLASVRTNVPAIFCSGGPMKAGLSAHGKALTL
SSVFEAVGAFKDGSMSQEDFLDMEANACPTCGSCAGMFTANSMNCLMEILGMAVPGNGTTLAVSDARRDLIRESAFHLMD
LVKKDIRPRDIITKDAIDDAFALDMAMGGSTNTVLHTLALANEAGIEDYDLERINDIAKRVPYLSKIAPSSSYSMHDVHE
AGGVSAIVKELVDLGGAIHPDRITVTGKTIRENVADAKINNTDVIHPKENPYSPVGGLSMLFGNIAPKGAAIKVGGVDPS
VQVFKGEAICFSSHDEAVEAIDNHTVREGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIA
VGHISPEAAAGGPIALVHDGDIITIDLPNRTLNVDVSDEVLEERRKELPKFKAKVKTGYLARYTALVTSAHTGGILQIPE
DLID
>Mature_564_residues
MRSDKIKKGVEQAPARSLLHATGQIKSPGDMDKPFIAICNSYIDIVPGHVHLRELADVAKEAIREAGGIPFEFNTIGVDD
GIAMGHIGMRYSLPSREVIADAAETVINAHWFDGVFYIPNCDKITPGMLLASVRTNVPAIFCSGGPMKAGLSAHGKALTL
SSVFEAVGAFKDGSMSQEDFLDMEANACPTCGSCAGMFTANSMNCLMEILGMAVPGNGTTLAVSDARRDLIRESAFHLMD
LVKKDIRPRDIITKDAIDDAFALDMAMGGSTNTVLHTLALANEAGIEDYDLERINDIAKRVPYLSKIAPSSSYSMHDVHE
AGGVSAIVKELVDLGGAIHPDRITVTGKTIRENVADAKINNTDVIHPKENPYSPVGGLSMLFGNIAPKGAAIKVGGVDPS
VQVFKGEAICFSSHDEAVEAIDNHTVREGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIA
VGHISPEAAAGGPIALVHDGDIITIDLPNRTLNVDVSDEVLEERRKELPKFKAKVKTGYLARYTALVTSAHTGGILQIPE
DLID

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=45.7236842105263, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1788157, Length=533, Percent_Identity=30.0187617260788, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI2367371, Length=526, Percent_Identity=30.7984790874525, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786464, Length=503, Percent_Identity=32.4055666003976, Blast_Score=193, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322476, Length=559, Percent_Identity=39.8926654740608, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59865; Mature: 59865

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDKIKKGVEQAPARSLLHATGQIKSPGDMDKPFIAICNSYIDIVPGHVHLRELADVAK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
EAIREAGGIPFEFNTIGVDDGIAMGHIGMRYSLPSREVIADAAETVINAHWFDGVFYIPN
HHHHHHCCCCEEEEEECCCCCCEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEECCC
CDKITPGMLLASVRTNVPAIFCSGGPMKAGLSAHGKALTLSSVFEAVGAFKDGSMSQEDF
CCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHH
LDMEANACPTCGSCAGMFTANSMNCLMEILGMAVPGNGTTLAVSDARRDLIRESAFHLMD
HCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHH
LVKKDIRPRDIITKDAIDDAFALDMAMGGSTNTVLHTLALANEAGIEDYDLERINDIAKR
HHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VPYLSKIAPSSSYSMHDVHEAGGVSAIVKELVDLGGAIHPDRITVTGKTIRENVADAKIN
CCHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCC
NTDVIHPKENPYSPVGGLSMLFGNIAPKGAAIKVGGVDPSVQVFKGEAICFSSHDEAVEA
CCEEECCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEECCEEEECCCHHHHHH
IDNHTVREGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIA
HHCCCEECCCEEEEEECCCCCCCCCCHHHCCCHHHHHCCCCCCEEEEECCCCCCCCCCEE
VGHISPEAAAGGPIALVHDGDIITIDLPNRTLNVDVSDEVLEERRKELPKFKAKVKTGYL
EECCCCCCCCCCCEEEEECCCEEEEECCCCEEEECCCHHHHHHHHHHCHHHHHHHHHCHH
ARYTALVTSAHTGGILQIPEDLID
HHHHHHHHHCCCCCEEECCHHHCC
>Mature Secondary Structure
MRSDKIKKGVEQAPARSLLHATGQIKSPGDMDKPFIAICNSYIDIVPGHVHLRELADVAK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
EAIREAGGIPFEFNTIGVDDGIAMGHIGMRYSLPSREVIADAAETVINAHWFDGVFYIPN
HHHHHHCCCCEEEEEECCCCCCEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEECCC
CDKITPGMLLASVRTNVPAIFCSGGPMKAGLSAHGKALTLSSVFEAVGAFKDGSMSQEDF
CCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHH
LDMEANACPTCGSCAGMFTANSMNCLMEILGMAVPGNGTTLAVSDARRDLIRESAFHLMD
HCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHH
LVKKDIRPRDIITKDAIDDAFALDMAMGGSTNTVLHTLALANEAGIEDYDLERINDIAKR
HHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VPYLSKIAPSSSYSMHDVHEAGGVSAIVKELVDLGGAIHPDRITVTGKTIRENVADAKIN
CCHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCC
NTDVIHPKENPYSPVGGLSMLFGNIAPKGAAIKVGGVDPSVQVFKGEAICFSSHDEAVEA
CCEEECCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEECCEEEECCCHHHHHH
IDNHTVREGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIA
HHCCCEECCCEEEEEECCCCCCCCCCHHHCCCHHHHHCCCCCCEEEEECCCCCCCCCCEE
VGHISPEAAAGGPIALVHDGDIITIDLPNRTLNVDVSDEVLEERRKELPKFKAKVKTGYL
EECCCCCCCCCCCEEEEECCCEEEEECCCCEEEECCCHHHHHHHHHHCHHHHHHHHHCHH
ARYTALVTSAHTGGILQIPEDLID
HHHHHHHHHCCCCCEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]