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The map label for this gene is trpE

Identifier: 226224234

GI number: 226224234

Start: 1658671

End: 1660035

Strand: Reverse

Name: trpE

Synonym: Lm4b_01644

Alternate gene names: 226224234

Gene position: 1660035-1658671 (Counterclockwise)

Preceding gene: 226224239

Following gene: 226224233

Centisome position: 56.99

GC content: 39.85

Gene sequence:

>1365_bases
ATGAGAAAAACGAAAAAAATAGATGCTGACACACTAACGGCTATTCTGGCGTTTCAAAGACTTTCAGGTACAGGAAAAAG
TTTGCTAGAGGGGGCGGCGAAAGATGCGGAGGCTGGACGTTATTCGATCATTGCTATTAATCCAGTCCATGAAATTAAAG
TATATCAACATGATTATTATATCGACGGTATACATCAAGTTGTCGCAGATCCTTTAAAAGAAATTGAATTATTTATCGAA
AAAGCAAAAGAAGATGAAGCAGATTTACCGCTTGATTCAGGTGCAATTGGTTATGTTGGTTATGATGTCATTGCGCTTTA
TGAGAATTTAGGCGAAATTCCAGTAGAAACTCGGGATATGCCAGATATTCGTTTTTATGTATATGAAAGTTTTGTGATTA
TGGATCATCAGGCGGAAGAACTTATTTTAGTGCAAGATAATTGCTATTCGGGAAGAAGTGAGGCGGAACTAGATAAGGCG
CTTGAATTAATGTTTACTGAACTTACAACGCCGAAAAAGGAAGAGCACAAGGCTGTCCATGTTCCCAAAATGACATACAA
GAGTAACTACACGAAAGAGGAGTACATGGGGTTAGTGAAAAAAGCAAAAACCTATATCCAAGAGGGTGACTTCTTTCAAA
TTGTTCTCTCGCAACGACTAGAAGCAGATTTCACCGTGACACCATTTGATTATTATCGGAAATTACGTTTATTAAATCCT
TCTCCTTATCTTTATTTCATTGATTTTGGGGATACAGTGCTAATTGGCTCTTCGCCAGAAAGTTTGATTAAAACGAAAGG
GCGGACGGTAATTACGAATCCTATTGCAGGAACAAGACGGCGCGGGGCAACGAAACAAGAAGATGAATTACTTGCTTCGG
AATTGTTAGCGGATGAAAAAGAATTGGCAGAGCACCGGATGTTAGTAGATCTTGGACGAAATGATATTGGGAAAATCGCC
GAAACTGGATCGGTGCATGTGCCTGTTTACCTTACTATTGAAAGATACCGTTTCTTAATGCATTTAGTTTCAGTAGTAGA
AGGAACCTTAAAACCCGGACTTACTGCAATGGATGCTCTTCGGTCGACGCTTCCGGCTGGTACAGTTAGCGGAGCACCGA
AAATAAGAGCCATGGAGCGAATCTATGAATGGGAAAATGTAAAACGTGGCCCATATGCTGGTGCGGTTGGTTATTTAACT
AAAAATGGTGATTCGGATTTCGCGCTTTCTATTAGAACAATGGTACTTCATAACGGAAAAGCTTATGTTCAAGCTGGTGC
AGGAATTGTCTATGATTCCGATCCAGAAAGCGAATACTTAGAAACATTACAAAAAGCAAAGGCACTTTTGGAGGTGGGAG
AATGA

Upstream 100 bases:

>100_bases
ATGAGGTGGCACCGCGATAACTCGTCCTCTGAAAAAGCATAATTTTGCGCTTTTTCAGAGGACTTTTTTTTATTTTAGTA
GAGAAGAAGGGATGATTAAG

Downstream 100 bases:

>100_bases
TTCTATTAATTGATAATTACGATTCTTTTACATTTAATTTAGAACAGTATTTAGCAGAATTTAGTGAAGTGGTTGTGAAA
CGGAATGATGCGACTGATTT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MRKTKKIDADTLTAILAFQRLSGTGKSLLEGAAKDAEAGRYSIIAINPVHEIKVYQHDYYIDGIHQVVADPLKEIELFIE
KAKEDEADLPLDSGAIGYVGYDVIALYENLGEIPVETRDMPDIRFYVYESFVIMDHQAEELILVQDNCYSGRSEAELDKA
LELMFTELTTPKKEEHKAVHVPKMTYKSNYTKEEYMGLVKKAKTYIQEGDFFQIVLSQRLEADFTVTPFDYYRKLRLLNP
SPYLYFIDFGDTVLIGSSPESLIKTKGRTVITNPIAGTRRRGATKQEDELLASELLADEKELAEHRMLVDLGRNDIGKIA
ETGSVHVPVYLTIERYRFLMHLVSVVEGTLKPGLTAMDALRSTLPAGTVSGAPKIRAMERIYEWENVKRGPYAGAVGYLT
KNGDSDFALSIRTMVLHNGKAYVQAGAGIVYDSDPESEYLETLQKAKALLEVGE

Sequences:

>Translated_454_residues
MRKTKKIDADTLTAILAFQRLSGTGKSLLEGAAKDAEAGRYSIIAINPVHEIKVYQHDYYIDGIHQVVADPLKEIELFIE
KAKEDEADLPLDSGAIGYVGYDVIALYENLGEIPVETRDMPDIRFYVYESFVIMDHQAEELILVQDNCYSGRSEAELDKA
LELMFTELTTPKKEEHKAVHVPKMTYKSNYTKEEYMGLVKKAKTYIQEGDFFQIVLSQRLEADFTVTPFDYYRKLRLLNP
SPYLYFIDFGDTVLIGSSPESLIKTKGRTVITNPIAGTRRRGATKQEDELLASELLADEKELAEHRMLVDLGRNDIGKIA
ETGSVHVPVYLTIERYRFLMHLVSVVEGTLKPGLTAMDALRSTLPAGTVSGAPKIRAMERIYEWENVKRGPYAGAVGYLT
KNGDSDFALSIRTMVLHNGKAYVQAGAGIVYDSDPESEYLETLQKAKALLEVGE
>Mature_454_residues
MRKTKKIDADTLTAILAFQRLSGTGKSLLEGAAKDAEAGRYSIIAINPVHEIKVYQHDYYIDGIHQVVADPLKEIELFIE
KAKEDEADLPLDSGAIGYVGYDVIALYENLGEIPVETRDMPDIRFYVYESFVIMDHQAEELILVQDNCYSGRSEAELDKA
LELMFTELTTPKKEEHKAVHVPKMTYKSNYTKEEYMGLVKKAKTYIQEGDFFQIVLSQRLEADFTVTPFDYYRKLRLLNP
SPYLYFIDFGDTVLIGSSPESLIKTKGRTVITNPIAGTRRRGATKQEDELLASELLADEKELAEHRMLVDLGRNDIGKIA
ETGSVHVPVYLTIERYRFLMHLVSVVEGTLKPGLTAMDALRSTLPAGTVSGAPKIRAMERIYEWENVKRGPYAGAVGYLT
KNGDSDFALSIRTMVLHNGKAYVQAGAGIVYDSDPESEYLETLQKAKALLEVGE

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=368, Percent_Identity=39.945652173913, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1788114, Length=441, Percent_Identity=31.7460317460317, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI87082077, Length=261, Percent_Identity=29.1187739463602, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786809, Length=219, Percent_Identity=31.0502283105023, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320935, Length=434, Percent_Identity=40.7834101382488, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6324361, Length=385, Percent_Identity=25.4545454545455, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 50935; Mature: 50935

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKKIDADTLTAILAFQRLSGTGKSLLEGAAKDAEAGRYSIIAINPVHEIKVYQHDYY
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCEE
IDGIHQVVADPLKEIELFIEKAKEDEADLPLDSGAIGYVGYDVIALYENLGEIPVETRDM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
PDIRFYVYESFVIMDHQAEELILVQDNCYSGRSEAELDKALELMFTELTTPKKEEHKAVH
CCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
VPKMTYKSNYTKEEYMGLVKKAKTYIQEGDFFQIVLSQRLEADFTVTPFDYYRKLRLLNP
CCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHEEEECC
SPYLYFIDFGDTVLIGSSPESLIKTKGRTVITNPIAGTRRRGATKQEDELLASELLADEK
CCEEEEEECCCEEEECCCHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHH
ELAEHRMLVDLGRNDIGKIAETGSVHVPVYLTIERYRFLMHLVSVVEGTLKPGLTAMDAL
HHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RSTLPAGTVSGAPKIRAMERIYEWENVKRGPYAGAVGYLTKNGDSDFALSIRTMVLHNGK
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCC
AYVQAGAGIVYDSDPESEYLETLQKAKALLEVGE
EEEEECCEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKTKKIDADTLTAILAFQRLSGTGKSLLEGAAKDAEAGRYSIIAINPVHEIKVYQHDYY
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCEE
IDGIHQVVADPLKEIELFIEKAKEDEADLPLDSGAIGYVGYDVIALYENLGEIPVETRDM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
PDIRFYVYESFVIMDHQAEELILVQDNCYSGRSEAELDKALELMFTELTTPKKEEHKAVH
CCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
VPKMTYKSNYTKEEYMGLVKKAKTYIQEGDFFQIVLSQRLEADFTVTPFDYYRKLRLLNP
CCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHEEEECC
SPYLYFIDFGDTVLIGSSPESLIKTKGRTVITNPIAGTRRRGATKQEDELLASELLADEK
CCEEEEEECCCEEEECCCHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHH
ELAEHRMLVDLGRNDIGKIAETGSVHVPVYLTIERYRFLMHLVSVVEGTLKPGLTAMDAL
HHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RSTLPAGTVSGAPKIRAMERIYEWENVKRGPYAGAVGYLTKNGDSDFALSIRTMVLHNGK
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCC
AYVQAGAGIVYDSDPESEYLETLQKAKALLEVGE
EEEEECCEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471 [H]