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The map label for this gene is citC

Identifier: 226224167

GI number: 226224167

Start: 1588311

End: 1589573

Strand: Reverse

Name: citC

Synonym: Lm4b_01577

Alternate gene names: 226224167

Gene position: 1589573-1588311 (Counterclockwise)

Preceding gene: 226224168

Following gene: 226224166

Centisome position: 54.57

GC content: 38.95

Gene sequence:

>1263_bases
ATGAGTCAAAAAATTCAAGTAGAAAACGGTGTGCTCAAAACACCAAACAACCCAGTCATTCCTTTTATTGAAGGGGACGG
AACTGGTCCAGACATCTGGGCAGCTGCAAAACGCGTTTTAGATGCCGCAGTAAAAAAAGCTTACAATGGCGAAAAAGAAA
TTGCTTGGAAAGAAGTATTAGCTGGTGAAAAAGCATTTAAACAAACAGGAGAATGGTTACCACAAGCGACTTTAGATACA
ATTGATGAATATTTGATTGCTATTAAAGGACCGCTTACTACTCCAATCGGTGGAGGCATTCGTTCGTTAAACGTTGCCCT
GCGCCAAGAGTTAGATTTATATGTTTGTTTACGTCCAGTTCGTTATTTCAAAGGCGTTCCTTCACCGGTGAAACGTCCGG
AAGACACAGATATGGTCATTTTCCGTGAGAATACAGAAGATATATATGCAGGGATTGAATTTAAAGAAGGCAGCGAAGAA
TCAAAAAAACTAATCGACTTCTTAAAATCTGAGTTTGGCGTTGACAAAATTCGTTTTCCGGAAACTTCTGGTATTGGTAT
TAAGCCAATTTCCAAAGAGGGAACAGAGCGTTTAGTGCGTTCGGCGATTGAATATGCAATTAAAGAAGATCGTAAATCAT
TAACGCTTGTACACAAAGGGAATATCATGAAGTTCACAGAAGGAGCTTTCAAAAATTGGGGCTATGATTTATGTGAACGC
GAATATGGTGATAAAGTATTTACTTGGAATGAGTATGATCGCATTAAAGAAGCTGAAGGAACAGAAGTTGCTGATGCGAA
ACAAAAAGAAGCGCTTGCTGCTGGGAAAATTTTAGTCAAAGATTCGATTGCGGATATTTTCTTGCAACAAATCTTAACTC
GCCCGGCTGAATTTGATGTAGTTGCTACAATGAACTTGAATGGAGATTACATTTCTGATGCACTTGCTGCTCAAGTAGGC
GGAATCGGTATTGCTCCAGGAGCAAACATTAACTACTTAACTGGCCATGCAATTTTCGAAGCGACGCATGGTACTGCACC
GAAATATGCAGGTCTTGATAAAGTGAATCCTTCATCAGTTATTTTATCTGGGACACTTCTTCTTGAACATATTGGTTGGG
GTGAAGCGGCTGCATTAATTAATCAGTCAATGGAAAAAACAATCGCTGCGAAAACCGTAACTTATGATTTTGCTCGATTA
ATGGATGGTGCGACAGAAGTTAAATGTTCGGAATTTGCAGATGAATTAATAAAAAATTTCTAA

Upstream 100 bases:

>100_bases
GATAATCGTCTTATTCGTCCGCGTGCAATTTACGTTGGACCTGAAAATAGAAGTTATTTACCTGTAGAAGAACGAATTTA
ATCGAGGAGGAACAAACTTT

Downstream 100 bases:

>100_bases
AACAATCCATTTTAACTTCTAGAACTAGTCTGTATTTTTATTTTTAACCATAAATATACGTTTTTAAAACAAACCATTTA
ATCCCATTCGGATAAGTGGT

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: IDH; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSQKIQVENGVLKTPNNPVIPFIEGDGTGPDIWAAAKRVLDAAVKKAYNGEKEIAWKEVLAGEKAFKQTGEWLPQATLDT
IDEYLIAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFKGVPSPVKRPEDTDMVIFRENTEDIYAGIEFKEGSEE
SKKLIDFLKSEFGVDKIRFPETSGIGIKPISKEGTERLVRSAIEYAIKEDRKSLTLVHKGNIMKFTEGAFKNWGYDLCER
EYGDKVFTWNEYDRIKEAEGTEVADAKQKEALAAGKILVKDSIADIFLQQILTRPAEFDVVATMNLNGDYISDALAAQVG
GIGIAPGANINYLTGHAIFEATHGTAPKYAGLDKVNPSSVILSGTLLLEHIGWGEAAALINQSMEKTIAAKTVTYDFARL
MDGATEVKCSEFADELIKNF

Sequences:

>Translated_420_residues
MSQKIQVENGVLKTPNNPVIPFIEGDGTGPDIWAAAKRVLDAAVKKAYNGEKEIAWKEVLAGEKAFKQTGEWLPQATLDT
IDEYLIAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFKGVPSPVKRPEDTDMVIFRENTEDIYAGIEFKEGSEE
SKKLIDFLKSEFGVDKIRFPETSGIGIKPISKEGTERLVRSAIEYAIKEDRKSLTLVHKGNIMKFTEGAFKNWGYDLCER
EYGDKVFTWNEYDRIKEAEGTEVADAKQKEALAAGKILVKDSIADIFLQQILTRPAEFDVVATMNLNGDYISDALAAQVG
GIGIAPGANINYLTGHAIFEATHGTAPKYAGLDKVNPSSVILSGTLLLEHIGWGEAAALINQSMEKTIAAKTVTYDFARL
MDGATEVKCSEFADELIKNF
>Mature_419_residues
SQKIQVENGVLKTPNNPVIPFIEGDGTGPDIWAAAKRVLDAAVKKAYNGEKEIAWKEVLAGEKAFKQTGEWLPQATLDTI
DEYLIAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRYFKGVPSPVKRPEDTDMVIFRENTEDIYAGIEFKEGSEES
KKLIDFLKSEFGVDKIRFPETSGIGIKPISKEGTERLVRSAIEYAIKEDRKSLTLVHKGNIMKFTEGAFKNWGYDLCERE
YGDKVFTWNEYDRIKEAEGTEVADAKQKEALAAGKILVKDSIADIFLQQILTRPAEFDVVATMNLNGDYISDALAAQVGG
IGIAPGANINYLTGHAIFEATHGTAPKYAGLDKVNPSSVILSGTLLLEHIGWGEAAALINQSMEKTIAAKTVTYDFARLM
DGATEVKCSEFADELIKNF

Specific function: Unknown

COG id: COG0538

COG function: function code C; Isocitrate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=403, Percent_Identity=31.7617866004963, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI28178821, Length=365, Percent_Identity=27.6712328767123, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178816, Length=365, Percent_Identity=27.6712328767123, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178838, Length=381, Percent_Identity=26.509186351706, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4758582, Length=370, Percent_Identity=27.027027027027, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=196, Percent_Identity=29.0816326530612, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1787381, Length=420, Percent_Identity=63.8095238095238, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1788101, Length=392, Percent_Identity=29.5918367346939, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI87081683, Length=412, Percent_Identity=26.4563106796117, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=372, Percent_Identity=30.3763440860215, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25144293, Length=382, Percent_Identity=27.2251308900524, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=375, Percent_Identity=27.4666666666667, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17550882, Length=409, Percent_Identity=24.4498777506112, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324709, Length=408, Percent_Identity=30.8823529411765, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=412, Percent_Identity=28.8834951456311, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322097, Length=378, Percent_Identity=29.8941798941799, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24643268, Length=398, Percent_Identity=32.1608040201005, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24661184, Length=402, Percent_Identity=31.8407960199005, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24643270, Length=398, Percent_Identity=32.1608040201005, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI161078639, Length=295, Percent_Identity=29.1525423728814, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161078637, Length=290, Percent_Identity=29.6551724137931, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078635, Length=290, Percent_Identity=29.6551724137931, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078633, Length=290, Percent_Identity=29.6551724137931, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24650122, Length=290, Percent_Identity=29.6551724137931, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI281362242, Length=400, Percent_Identity=26.75, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648872, Length=400, Percent_Identity=26.75, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 8180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004439 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.42 [H]

Molecular weight: Translated: 46236; Mature: 46105

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKIQVENGVLKTPNNPVIPFIEGDGTGPDIWAAAKRVLDAAVKKAYNGEKEIAWKEVL
CCCCEEECCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AGEKAFKQTGEWLPQATLDTIDEYLIAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPV
HCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
RYFKGVPSPVKRPEDTDMVIFRENTEDIYAGIEFKEGSEESKKLIDFLKSEFGVDKIRFP
HHHCCCCCCCCCCCCCCEEEEECCCCEEEECEEECCCCHHHHHHHHHHHHHCCCCCEECC
ETSGIGIKPISKEGTERLVRSAIEYAIKEDRKSLTLVHKGNIMKFTEGAFKNWGYDLCER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHCCHHHHHH
EYGDKVFTWNEYDRIKEAEGTEVADAKQKEALAAGKILVKDSIADIFLQQILTRPAEFDV
HHCCCEEEECHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
VATMNLNGDYISDALAAQVGGIGIAPGANINYLTGHAIFEATHGTAPKYAGLDKVNPSSV
EEEEECCCHHHHHHHHHHHCCEEECCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCE
ILSGTLLLEHIGWGEAAALINQSMEKTIAAKTVTYDFARLMDGATEVKCSEFADELIKNF
EEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SQKIQVENGVLKTPNNPVIPFIEGDGTGPDIWAAAKRVLDAAVKKAYNGEKEIAWKEVL
CCCEEECCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AGEKAFKQTGEWLPQATLDTIDEYLIAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPV
HCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
RYFKGVPSPVKRPEDTDMVIFRENTEDIYAGIEFKEGSEESKKLIDFLKSEFGVDKIRFP
HHHCCCCCCCCCCCCCCEEEEECCCCEEEECEEECCCCHHHHHHHHHHHHHCCCCCEECC
ETSGIGIKPISKEGTERLVRSAIEYAIKEDRKSLTLVHKGNIMKFTEGAFKNWGYDLCER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHCCHHHHHH
EYGDKVFTWNEYDRIKEAEGTEVADAKQKEALAAGKILVKDSIADIFLQQILTRPAEFDV
HHCCCEEEECHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
VATMNLNGDYISDALAAQVGGIGIAPGANINYLTGHAIFEATHGTAPKYAGLDKVNPSSV
EEEEECCCHHHHHHHHHHHCCEEECCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCE
ILSGTLLLEHIGWGEAAALINQSMEKTIAAKTVTYDFARLMDGATEVKCSEFADELIKNF
EEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377; 11751849; 11290745 [H]