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The map label for this gene is citZ

Identifier: 226224168

GI number: 226224168

Start: 1589593

End: 1590714

Strand: Reverse

Name: citZ

Synonym: Lm4b_01578

Alternate gene names: 226224168

Gene position: 1590714-1589593 (Counterclockwise)

Preceding gene: 226224169

Following gene: 226224167

Centisome position: 54.61

GC content: 39.3

Gene sequence:

>1122_bases
ATGACGTTATCTAAAGGCTTAGAAAATGTATATGTTGCTGAAACTTCCATTAGCTCTATCATTGATGATATGTTAACTTA
TGTTGGCTATGGTATTGACGATTTAATGGAAAATAACGCTTCTTTTGAAGAAGTGATTTACTTATTATGGCATTTACGTT
TGCCGAATAAAGATGAATTTAAAAAATTTAAACGAGAAATCCAAGAAAATATGGCAGTGTCTGAAACAATCATTACGAGC
TTAAGAATGCAAAATCACCAAAAATTACATCCGATGAGTGTTTTAAGAACTACAGTTTCTATGCTCGGTGTATTTGATAC
AGAAGCTGAATTAGACGACGGGGAAGCAGTTTACCGTAAAGGTTTGCGACTTCAAGCGAAAATGCCAACCATTGTTGCCG
CTTTTTCCAGAATTCGTCGCGGACTCGATCCAATTCCACCGAGAGAAGATTTAAATATGGCAGCAAATTTCCTATATATG
ATTACAGGAGAAGAAGCAGATGCATTATCAGTAGAAGCAATGAATAAAGCGCTCGTACTGCACGCTGATCACGAATTTAA
TGCGTCTACTTTTACAGCGCGAGTTTGTGTGGCAACGCTTTCGGATGTTTATTCCGGCGTAACAGCTGCAATCGGTGCGC
TGAAAGGCCCGCTTCATGGTGGTGCCAATGAACGCGTATTTGATATGTTAGAAGAAATTGACCAAGCTGGTGATGTGCGT
AATTACATTCAAGAAAAAATTGATACGAAGCAAAAGATTATGGGATTTGGTCACCGTGTTTATCGCGGCGGAGATCCTCG
TGCGCAACATTTGCGTGAAATGTCCAGAAAGTTAACTGCTGAAAAAGGCGAAGAGAAATGGTTTGATATTTCGCTGCAAG
TAGAAGAATCTGTTTGGGAATTGAAACACTTAAAACCGAATGTCGATTTCTATTCAGCCTCCGTTTATCATGCGCTTGGT
ATTGACCGCGACTTGTTTACGCTTGTGTTTTCTGTAAGTCGTGTATCTGGTTGGTTAGCACACATTTTCGAGCAATACCG
TGATAATCGTCTTATTCGTCCGCGTGCAATTTACGTTGGACCTGAAAATAGAAGTTATTTACCTGTAGAAGAACGAATTT
AA

Upstream 100 bases:

>100_bases
TGGACCAGTAATTGCAGCTGGTATTGCGTACATGGCAATGCAGTTAGTTGCATTCATAAAATAAGTACATACGTAAAAAA
GGATAAAAGGAGGAAATGAA

Downstream 100 bases:

>100_bases
TCGAGGAGGAACAAACTTTATGAGTCAAAAAATTCAAGTAGAAAACGGTGTGCTCAAAACACCAAACAACCCAGTCATTC
CTTTTATTGAAGGGGACGGA

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTLSKGLENVYVAETSISSIIDDMLTYVGYGIDDLMENNASFEEVIYLLWHLRLPNKDEFKKFKREIQENMAVSETIITS
LRMQNHQKLHPMSVLRTTVSMLGVFDTEAELDDGEAVYRKGLRLQAKMPTIVAAFSRIRRGLDPIPPREDLNMAANFLYM
ITGEEADALSVEAMNKALVLHADHEFNASTFTARVCVATLSDVYSGVTAAIGALKGPLHGGANERVFDMLEEIDQAGDVR
NYIQEKIDTKQKIMGFGHRVYRGGDPRAQHLREMSRKLTAEKGEEKWFDISLQVEESVWELKHLKPNVDFYSASVYHALG
IDRDLFTLVFSVSRVSGWLAHIFEQYRDNRLIRPRAIYVGPENRSYLPVEERI

Sequences:

>Translated_373_residues
MTLSKGLENVYVAETSISSIIDDMLTYVGYGIDDLMENNASFEEVIYLLWHLRLPNKDEFKKFKREIQENMAVSETIITS
LRMQNHQKLHPMSVLRTTVSMLGVFDTEAELDDGEAVYRKGLRLQAKMPTIVAAFSRIRRGLDPIPPREDLNMAANFLYM
ITGEEADALSVEAMNKALVLHADHEFNASTFTARVCVATLSDVYSGVTAAIGALKGPLHGGANERVFDMLEEIDQAGDVR
NYIQEKIDTKQKIMGFGHRVYRGGDPRAQHLREMSRKLTAEKGEEKWFDISLQVEESVWELKHLKPNVDFYSASVYHALG
IDRDLFTLVFSVSRVSGWLAHIFEQYRDNRLIRPRAIYVGPENRSYLPVEERI
>Mature_372_residues
TLSKGLENVYVAETSISSIIDDMLTYVGYGIDDLMENNASFEEVIYLLWHLRLPNKDEFKKFKREIQENMAVSETIITSL
RMQNHQKLHPMSVLRTTVSMLGVFDTEAELDDGEAVYRKGLRLQAKMPTIVAAFSRIRRGLDPIPPREDLNMAANFLYMI
TGEEADALSVEAMNKALVLHADHEFNASTFTARVCVATLSDVYSGVTAAIGALKGPLHGGANERVFDMLEEIDQAGDVRN
YIQEKIDTKQKIMGFGHRVYRGGDPRAQHLREMSRKLTAEKGEEKWFDISLQVEESVWELKHLKPNVDFYSASVYHALGI
DRDLFTLVFSVSRVSGWLAHIFEQYRDNRLIRPRAIYVGPENRSYLPVEERI

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=342, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786527, Length=377, Percent_Identity=36.3395225464191, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1786939, Length=384, Percent_Identity=33.0729166666667, Blast_Score=207, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17555174, Length=344, Percent_Identity=28.1976744186047, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319850, Length=328, Percent_Identity=25.609756097561, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=328, Percent_Identity=25.3048780487805, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325257, Length=195, Percent_Identity=29.2307692307692, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640126, Length=323, Percent_Identity=29.4117647058824, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640124, Length=323, Percent_Identity=29.4117647058824, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356863, Length=327, Percent_Identity=27.82874617737, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 42499; Mature: 42368

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSKGLENVYVAETSISSIIDDMLTYVGYGIDDLMENNASFEEVIYLLWHLRLPNKDEF
CCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
KKFKREIQENMAVSETIITSLRMQNHQKLHPMSVLRTTVSMLGVFDTEAELDDGEAVYRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GLRLQAKMPTIVAAFSRIRRGLDPIPPREDLNMAANFLYMITGEEADALSVEAMNKALVL
CCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHCCEEEE
HADHEFNASTFTARVCVATLSDVYSGVTAAIGALKGPLHGGANERVFDMLEEIDQAGDVR
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHH
NYIQEKIDTKQKIMGFGHRVYRGGDPRAQHLREMSRKLTAEKGEEKWFDISLQVEESVWE
HHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHH
LKHLKPNVDFYSASVYHALGIDRDLFTLVFSVSRVSGWLAHIFEQYRDNRLIRPRAIYVG
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PENRSYLPVEERI
CCCCCCCCHHCCC
>Mature Secondary Structure 
TLSKGLENVYVAETSISSIIDDMLTYVGYGIDDLMENNASFEEVIYLLWHLRLPNKDEF
CCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
KKFKREIQENMAVSETIITSLRMQNHQKLHPMSVLRTTVSMLGVFDTEAELDDGEAVYRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GLRLQAKMPTIVAAFSRIRRGLDPIPPREDLNMAANFLYMITGEEADALSVEAMNKALVL
CCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHCCEEEE
HADHEFNASTFTARVCVATLSDVYSGVTAAIGALKGPLHGGANERVFDMLEEIDQAGDVR
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHH
NYIQEKIDTKQKIMGFGHRVYRGGDPRAQHLREMSRKLTAEKGEEKWFDISLQVEESVWE
HHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHH
LKHLKPNVDFYSASVYHALGIDRDLFTLVFSVSRVSGWLAHIFEQYRDNRLIRPRAIYVG
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PENRSYLPVEERI
CCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]