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The map label for this gene is polA

Identifier: 226224166

GI number: 226224166

Start: 1585519

End: 1588146

Strand: Reverse

Name: polA

Synonym: Lm4b_01576

Alternate gene names: 226224166

Gene position: 1588146-1585519 (Counterclockwise)

Preceding gene: 226224167

Following gene: 226224165

Centisome position: 54.53

GC content: 37.63

Gene sequence:

>2628_bases
GTGAATAAATTAGTATTAATTGATGGAAATAGTATTGCGAATCGCGCCTTTTATGCTTTACCGCTTTTAAATAATGATAA
AGGTGTTTACACAAATGCGGTGTATGGTTTCGCAATGATGTTGATGAATGTACTCGAAAAAGAAAAACCTAGCCATGTGC
TCGTTGCATTTGATGCTGGGAAAACAACTTTCAGACATACAACATTTAAAGGTTATAAAGGCGGACGTCAAAAAACCCCA
AGTGAGTTATCTGAACAGTTTCCATTCATCCGAGAACTTTTAACGGCGTATGATATCCCGCAATATGAACTTGCCAATTA
TGAGGCAGATGATATTATCGGAACATTGACGAAAAAAGCGGAAGCAGACGGAATGGAAGTAGCAATCATTACCGGAGACC
GGGACTTAACGCAATTAGCTTCTGAAAAAACAACGGTTTATATTACTAAAAAAGGCATAGCCGATATGGAAACCAACACA
CCAGAAACACTAAAAGAAAAATACAATCTGACTCCTCAACAAATCATTGATATGAAGGGTTTAATGGGTGATTCCTCCGA
TAATATTCCTGGTATTCCAGGCGTTGGTGAAAAAACTGCCTTAAAACTCTTACATGAATTTGGTGGAACGGTGGAAAGTG
TGTTAGAGCATGCCGAGGAAATTTCCGGTAAAAAACTAAAAGAAAAAGTACTGGAAAATAAAGAATTAGCTATTTTAAGT
AAAGAGTTAGCTACAATCAATGTGGATTCACCGATTGAATGCACGGTCGAAAGCACGAAATACGACGGCTATCAAACAGA
AAAAGTTATCCCTTTATTAAAAGAAATGAATTTTACAACACTTTTGAAAAATATCGAGGGAGATACACCAGAAGAAACGA
AAGAACTAAAAGAGTTGTCTTTTGAAATCGTAACAGAATTAAAAGAAAAACATTTTGGTGGGAAAACCGCGCTTTATGTG
GAGCTAGAAAACGATAATTACCATACAGCTAATTTCATTGGATTAACAGTACATTCTAGCCAAGGGACGTATTTCTTTAC
AAAAGAAACAGCCGAAAGTAGTGAGGTATTCCGCAAATGGGTAGAAAGTGATCAAACAAAAGTAGTGTATGATGCGAAGA
AAACAATGGTTGCAATGAACCGCATTGGTTTGGCCATTTCTGGTATCACCTTTGATATCATGTTAGCATCTTACTTGCTA
AATCCATCTGATTCGATTGATGACTTTACAAGTGTTGCAATACGACATGATTACACAGAAGTAGAAACAGATGAAGCAGT
CTACGGAAAAGGTGCTAAACGTAGCACTCCTGAAGAAAGCATTGTTGCGGCACACTTAGCTAGAAAAGCAGTGGCTATTG
CTGACTTAGAGAAAAGACTAGTTGATGATTTAGAAGAAAATGAACAACTAGCTTTGATGGAAGACCTTGAGCTACCTCTA
ACTTTTGTATTAGCAGAAATGGAAATGCAAGGGGTTAGTGTAGATACAGAACGTCTAGAGAATATGAAAGTGGAGCTAGC
GGGTAGACTGAAAACATTGGAAGAGTCGATCCATTCTCTAGCCGGCGAAGAATTCAATATTAATTCACCGAAACAACTAG
GTGTGATTTTATTTGAAAAGCTTGGTCTCCCAGCTGTCAAGAAAACAAAAACAGGTTATTCAACGGCTGCCGATGTACTG
GAAAGTTTATCTGGTAAACACGCGATTATTGATGAAATTTTGTTATATCGTCAACTTGGTAAAATTCAGTCCACTTATAT
CGAAGGGCTGCTTAAAGTGACAGATAAAGAAACACATAAAGTACACACACGTTTCAACCAAACATTAACGCAAACAGGAC
GCTTAAGTTCAGTTGATCCGAACTTGCAAAATATCCCTATTCGTTTAGAAGAAGGACGGAAAATTAGACAAGTTTTCGTA
CCGAGTAAACCAGGTTGGAAAATGTTTTCAGCCGATTATTCTCAAGTAGAGCTGAGAGTCTTAGCACATATTTCGGAAGA
TGAAAATTTAATTTATGCATTTAAGCACGATTATGATATTCATACGAAAACGGCGATGGACGTGTTTCATGTTGAGCAAG
ACGAAGTGGATTCATTAATGCGTCGTCAGGCTAAAGCGGTAAACTTTGGTATTGTATATGGAATTAGTGATTATGGGCTT
TCGCAAAACCTTGGTATTACGCGTAAAGAAGCAAAAGACTTTATTGATCGTTATTTTGTGAGCTATCCAGCTGTGAAAGA
ATATATGCAAGATATCGTTCGTTTTGCGAAAGAAAAAGGCTATGTAGAGACCATTTTACATCGTCGTCGGTATATTCCAG
AGATTGTCAGTCGTAATTTTAATGTTCGCGGATTTGCAGAAAGAACGGCGATGAACACGCCTATTCAAGGTAGTGCCGCT
GATATTATCAAAAAAGCGATGATTCTGATGAGCGAGCGTTTAGTATCTGAAAAATTAGAAGCAAAATTATTACTACAAGT
ACACGATGAATTAATCTTCGAGGCGCCTGAAGCTGAAATCGCTAAATTAGAAGAAATTGTCCCAGATGTTATGGAAAATG
CAGTAGAGCTTTCTGTACCGCTAAAAGTAGATAGCGCATTTGGTGACACTTGGTACGACGCGAAATAA

Upstream 100 bases:

>100_bases
TAAAACAAACCATTTAATCCCATTCGGATAAGTGGTTTGTTTTTATGTTCCTATGGTAAAATAGACACAAGTGAATGACA
ATATCGAGGAGGATATCCCT

Downstream 100 bases:

>100_bases
ATAGAGAAACGGGGAGTTTATATATGCCAGAAATGCCGGAAGTAGAAAATGTCCGCGCAACTTTACAAGAATTAGTCCCA
GGTAAAAAAATAGATCAAGT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 875; Mature: 875

Protein sequence:

>875_residues
MNKLVLIDGNSIANRAFYALPLLNNDKGVYTNAVYGFAMMLMNVLEKEKPSHVLVAFDAGKTTFRHTTFKGYKGGRQKTP
SELSEQFPFIRELLTAYDIPQYELANYEADDIIGTLTKKAEADGMEVAIITGDRDLTQLASEKTTVYITKKGIADMETNT
PETLKEKYNLTPQQIIDMKGLMGDSSDNIPGIPGVGEKTALKLLHEFGGTVESVLEHAEEISGKKLKEKVLENKELAILS
KELATINVDSPIECTVESTKYDGYQTEKVIPLLKEMNFTTLLKNIEGDTPEETKELKELSFEIVTELKEKHFGGKTALYV
ELENDNYHTANFIGLTVHSSQGTYFFTKETAESSEVFRKWVESDQTKVVYDAKKTMVAMNRIGLAISGITFDIMLASYLL
NPSDSIDDFTSVAIRHDYTEVETDEAVYGKGAKRSTPEESIVAAHLARKAVAIADLEKRLVDDLEENEQLALMEDLELPL
TFVLAEMEMQGVSVDTERLENMKVELAGRLKTLEESIHSLAGEEFNINSPKQLGVILFEKLGLPAVKKTKTGYSTAADVL
ESLSGKHAIIDEILLYRQLGKIQSTYIEGLLKVTDKETHKVHTRFNQTLTQTGRLSSVDPNLQNIPIRLEEGRKIRQVFV
PSKPGWKMFSADYSQVELRVLAHISEDENLIYAFKHDYDIHTKTAMDVFHVEQDEVDSLMRRQAKAVNFGIVYGISDYGL
SQNLGITRKEAKDFIDRYFVSYPAVKEYMQDIVRFAKEKGYVETILHRRRYIPEIVSRNFNVRGFAERTAMNTPIQGSAA
DIIKKAMILMSERLVSEKLEAKLLLQVHDELIFEAPEAEIAKLEEIVPDVMENAVELSVPLKVDSAFGDTWYDAK

Sequences:

>Translated_875_residues
MNKLVLIDGNSIANRAFYALPLLNNDKGVYTNAVYGFAMMLMNVLEKEKPSHVLVAFDAGKTTFRHTTFKGYKGGRQKTP
SELSEQFPFIRELLTAYDIPQYELANYEADDIIGTLTKKAEADGMEVAIITGDRDLTQLASEKTTVYITKKGIADMETNT
PETLKEKYNLTPQQIIDMKGLMGDSSDNIPGIPGVGEKTALKLLHEFGGTVESVLEHAEEISGKKLKEKVLENKELAILS
KELATINVDSPIECTVESTKYDGYQTEKVIPLLKEMNFTTLLKNIEGDTPEETKELKELSFEIVTELKEKHFGGKTALYV
ELENDNYHTANFIGLTVHSSQGTYFFTKETAESSEVFRKWVESDQTKVVYDAKKTMVAMNRIGLAISGITFDIMLASYLL
NPSDSIDDFTSVAIRHDYTEVETDEAVYGKGAKRSTPEESIVAAHLARKAVAIADLEKRLVDDLEENEQLALMEDLELPL
TFVLAEMEMQGVSVDTERLENMKVELAGRLKTLEESIHSLAGEEFNINSPKQLGVILFEKLGLPAVKKTKTGYSTAADVL
ESLSGKHAIIDEILLYRQLGKIQSTYIEGLLKVTDKETHKVHTRFNQTLTQTGRLSSVDPNLQNIPIRLEEGRKIRQVFV
PSKPGWKMFSADYSQVELRVLAHISEDENLIYAFKHDYDIHTKTAMDVFHVEQDEVDSLMRRQAKAVNFGIVYGISDYGL
SQNLGITRKEAKDFIDRYFVSYPAVKEYMQDIVRFAKEKGYVETILHRRRYIPEIVSRNFNVRGFAERTAMNTPIQGSAA
DIIKKAMILMSERLVSEKLEAKLLLQVHDELIFEAPEAEIAKLEEIVPDVMENAVELSVPLKVDSAFGDTWYDAK
>Mature_875_residues
MNKLVLIDGNSIANRAFYALPLLNNDKGVYTNAVYGFAMMLMNVLEKEKPSHVLVAFDAGKTTFRHTTFKGYKGGRQKTP
SELSEQFPFIRELLTAYDIPQYELANYEADDIIGTLTKKAEADGMEVAIITGDRDLTQLASEKTTVYITKKGIADMETNT
PETLKEKYNLTPQQIIDMKGLMGDSSDNIPGIPGVGEKTALKLLHEFGGTVESVLEHAEEISGKKLKEKVLENKELAILS
KELATINVDSPIECTVESTKYDGYQTEKVIPLLKEMNFTTLLKNIEGDTPEETKELKELSFEIVTELKEKHFGGKTALYV
ELENDNYHTANFIGLTVHSSQGTYFFTKETAESSEVFRKWVESDQTKVVYDAKKTMVAMNRIGLAISGITFDIMLASYLL
NPSDSIDDFTSVAIRHDYTEVETDEAVYGKGAKRSTPEESIVAAHLARKAVAIADLEKRLVDDLEENEQLALMEDLELPL
TFVLAEMEMQGVSVDTERLENMKVELAGRLKTLEESIHSLAGEEFNINSPKQLGVILFEKLGLPAVKKTKTGYSTAADVL
ESLSGKHAIIDEILLYRQLGKIQSTYIEGLLKVTDKETHKVHTRFNQTLTQTGRLSSVDPNLQNIPIRLEEGRKIRQVFV
PSKPGWKMFSADYSQVELRVLAHISEDENLIYAFKHDYDIHTKTAMDVFHVEQDEVDSLMRRQAKAVNFGIVYGISDYGL
SQNLGITRKEAKDFIDRYFVSYPAVKEYMQDIVRFAKEKGYVETILHRRRYIPEIVSRNFNVRGFAERTAMNTPIQGSAA
DIIKKAMILMSERLVSEKLEAKLLLQVHDELIFEAPEAEIAKLEEIVPDVMENAVELSVPLKVDSAFGDTWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=517, Percent_Identity=32.6885880077369, Blast_Score=243, Evalue=6e-64,
Organism=Homo sapiens, GI139394648, Length=687, Percent_Identity=25.3275109170306, Blast_Score=212, Evalue=1e-54,
Organism=Escherichia coli, GI1790294, Length=933, Percent_Identity=38.7995712754555, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI226510970, Length=224, Percent_Identity=29.0178571428571, Blast_Score=106, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI86563272, Length=393, Percent_Identity=30.5343511450382, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17933644, Length=420, Percent_Identity=33.8095238095238, Blast_Score=234, Evalue=3e-61,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98497; Mature: 98497

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLVLIDGNSIANRAFYALPLLNNDKGVYTNAVYGFAMMLMNVLEKEKPSHVLVAFDAG
CCCEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
KTTFRHTTFKGYKGGRQKTPSELSEQFPFIRELLTAYDIPQYELANYEADDIIGTLTKKA
CCEEEECCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHC
EADGMEVAIITGDRDLTQLASEKTTVYITKKGIADMETNTPETLKEKYNLTPQQIIDMKG
CCCCCEEEEEECCCHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
LMGDSSDNIPGIPGVGEKTALKLLHEFGGTVESVLEHAEEISGKKLKEKVLENKELAILS
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCHHEEEE
KELATINVDSPIECTVESTKYDGYQTEKVIPLLKEMNFTTLLKNIEGDTPEETKELKELS
HHHEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHH
FEIVTELKEKHFGGKTALYVELENDNYHTANFIGLTVHSSQGTYFFTKETAESSEVFRKW
HHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEEEEEECCCCCEEEEECCCCHHHHHHHH
VESDQTKVVYDAKKTMVAMNRIGLAISGITFDIMLASYLLNPSDSIDDFTSVAIRHDYTE
HCCCCCEEEEEHHHHHHHHHHHCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
VETDEAVYGKGAKRSTPEESIVAAHLARKAVAIADLEKRLVDDLEENEQLALMEDLELPL
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCH
TFVLAEMEMQGVSVDTERLENMKVELAGRLKTLEESIHSLAGEEFNINSPKQLGVILFEK
HHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LGLPAVKKTKTGYSTAADVLESLSGKHAIIDEILLYRQLGKIQSTYIEGLLKVTDKETHK
CCCCCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VHTRFNQTLTQTGRLSSVDPNLQNIPIRLEEGRKIRQVFVPSKPGWKMFSADYSQVELRV
HHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCEEECCCHHHHEEEE
LAHISEDENLIYAFKHDYDIHTKTAMDVFHVEQDEVDSLMRRQAKAVNFGIVYGISDYGL
EEECCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCC
SQNLGITRKEAKDFIDRYFVSYPAVKEYMQDIVRFAKEKGYVETILHRRRYIPEIVSRNF
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
NVRGFAERTAMNTPIQGSAADIIKKAMILMSERLVSEKLEAKLLLQVHDELIFEAPEAEI
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AKLEEIVPDVMENAVELSVPLKVDSAFGDTWYDAK
HHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNKLVLIDGNSIANRAFYALPLLNNDKGVYTNAVYGFAMMLMNVLEKEKPSHVLVAFDAG
CCCEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
KTTFRHTTFKGYKGGRQKTPSELSEQFPFIRELLTAYDIPQYELANYEADDIIGTLTKKA
CCEEEECCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHC
EADGMEVAIITGDRDLTQLASEKTTVYITKKGIADMETNTPETLKEKYNLTPQQIIDMKG
CCCCCEEEEEECCCHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
LMGDSSDNIPGIPGVGEKTALKLLHEFGGTVESVLEHAEEISGKKLKEKVLENKELAILS
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCHHEEEE
KELATINVDSPIECTVESTKYDGYQTEKVIPLLKEMNFTTLLKNIEGDTPEETKELKELS
HHHEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHH
FEIVTELKEKHFGGKTALYVELENDNYHTANFIGLTVHSSQGTYFFTKETAESSEVFRKW
HHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEEEEEECCCCCEEEEECCCCHHHHHHHH
VESDQTKVVYDAKKTMVAMNRIGLAISGITFDIMLASYLLNPSDSIDDFTSVAIRHDYTE
HCCCCCEEEEEHHHHHHHHHHHCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
VETDEAVYGKGAKRSTPEESIVAAHLARKAVAIADLEKRLVDDLEENEQLALMEDLELPL
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCH
TFVLAEMEMQGVSVDTERLENMKVELAGRLKTLEESIHSLAGEEFNINSPKQLGVILFEK
HHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LGLPAVKKTKTGYSTAADVLESLSGKHAIIDEILLYRQLGKIQSTYIEGLLKVTDKETHK
CCCCCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VHTRFNQTLTQTGRLSSVDPNLQNIPIRLEEGRKIRQVFVPSKPGWKMFSADYSQVELRV
HHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCEEECCCHHHHEEEE
LAHISEDENLIYAFKHDYDIHTKTAMDVFHVEQDEVDSLMRRQAKAVNFGIVYGISDYGL
EEECCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCC
SQNLGITRKEAKDFIDRYFVSYPAVKEYMQDIVRFAKEKGYVETILHRRRYIPEIVSRNF
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
NVRGFAERTAMNTPIQGSAADIIKKAMILMSERLVSEKLEAKLLLQVHDELIFEAPEAEI
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AKLEEIVPDVMENAVELSVPLKVDSAFGDTWYDAK
HHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]