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The map label for this gene is hemB

Identifier: 226224155

GI number: 226224155

Start: 1574176

End: 1575150

Strand: Reverse

Name: hemB

Synonym: Lm4b_01565

Alternate gene names: 226224155

Gene position: 1575150-1574176 (Counterclockwise)

Preceding gene: 226224156

Following gene: 226224154

Centisome position: 54.08

GC content: 37.33

Gene sequence:

>975_bases
ATGAAAAATCAATTTGATAGACATCGTCGTCTAAGAAAAACCAAAACAATGCGTGATTTAGTAAGAGAAACAGTTTTACA
TACAGATGATTTAATCTATCCGATTTTTGTTAAAGATGGCAAAGAACCAAAAACAGAAGTAGCTTCTATGCCTGGGGTTT
TTCAATATCCTTTACATGAACTAGAAGAAGAAATGCGCGTAGTTGAGAGCCTTGGTATTAAAGCGGTTATTTTATTTGGT
ATTCCTGCCGAGAAAGACGCAGTTGGCACGCAAGCTTATCACGACCACGGAATTATTCAAGAAGCTACAAGGCTTATAAA
AAAAAGTTTTCCTGAAATTCTTGTGGTGGCTGACACATGTTTATGTGAATTTACTGATCATGGACATTGTGGTGTTATTG
AAAACGGGGAAATTCTCAATGACGAATCGCTTGAATTATTGAAACAAACAGCTGTCAGTCAAGCCGCTGCAGGAGCGGAT
ATCATTGCTCCATCTAATATGATGGATGGTTTTGTTCAAGTCATTCGCGAAGGGTTAGATGAAGCGGGATTTTATGATAT
CCCAATTATGTCATATGCAGTTAAATATGCGTCTGCTTTTTATGGACCGTTCCGTGATGCTGCTGGTAGTGCCCCTCAAT
TTGGTGATAGAAAATCTTATCAAATGGATCCTGCCAATCGTGAAGAAGCTCTTCGCGAAGCAATGTCGGATGAACAAGAA
GGTGCAGATTTCCTTATTGTTAAACCATCATTATCATACTTAGATATCATGCGCGATGTTAAGAACAATACCAATTTACC
AGTAGTTGCCTACAATGTTAGTGGTGAATATGCAATGGTAAAAGCAGCTGCGCAAAATGGTTGGATAGATGAAGAAAAAA
TTGTTTTAGAAATGCTAACAAGTATGAAACGAGCGGGTGCAACACTTATCATTACTTATTTTGCAAAAGATGCATCCAAA
TATTTAAATAAATAG

Upstream 100 bases:

>100_bases
CAACAGAAGCCATTATAGCAGATGGATGGGAAGTAAAATATCAACCAAAAACCTTCACCATGAAGCACCTAGCTGATTTG
ATAATACAGGAGGAATTAAA

Downstream 100 bases:

>100_bases
GAGGTAAGAAATTTGCAAAACTATTCGAAATCCGAAAAGGCCTTTAAAGAAGCCAAAAAAGTCTTACCTGGTGGTGTAAA
TAGCCCTGTTCGAGCATTCA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKNQFDRHRRLRKTKTMRDLVRETVLHTDDLIYPIFVKDGKEPKTEVASMPGVFQYPLHELEEEMRVVESLGIKAVILFG
IPAEKDAVGTQAYHDHGIIQEATRLIKKSFPEILVVADTCLCEFTDHGHCGVIENGEILNDESLELLKQTAVSQAAAGAD
IIAPSNMMDGFVQVIREGLDEAGFYDIPIMSYAVKYASAFYGPFRDAAGSAPQFGDRKSYQMDPANREEALREAMSDEQE
GADFLIVKPSLSYLDIMRDVKNNTNLPVVAYNVSGEYAMVKAAAQNGWIDEEKIVLEMLTSMKRAGATLIITYFAKDASK
YLNK

Sequences:

>Translated_324_residues
MKNQFDRHRRLRKTKTMRDLVRETVLHTDDLIYPIFVKDGKEPKTEVASMPGVFQYPLHELEEEMRVVESLGIKAVILFG
IPAEKDAVGTQAYHDHGIIQEATRLIKKSFPEILVVADTCLCEFTDHGHCGVIENGEILNDESLELLKQTAVSQAAAGAD
IIAPSNMMDGFVQVIREGLDEAGFYDIPIMSYAVKYASAFYGPFRDAAGSAPQFGDRKSYQMDPANREEALREAMSDEQE
GADFLIVKPSLSYLDIMRDVKNNTNLPVVAYNVSGEYAMVKAAAQNGWIDEEKIVLEMLTSMKRAGATLIITYFAKDASK
YLNK
>Mature_324_residues
MKNQFDRHRRLRKTKTMRDLVRETVLHTDDLIYPIFVKDGKEPKTEVASMPGVFQYPLHELEEEMRVVESLGIKAVILFG
IPAEKDAVGTQAYHDHGIIQEATRLIKKSFPEILVVADTCLCEFTDHGHCGVIENGEILNDESLELLKQTAVSQAAAGAD
IIAPSNMMDGFVQVIREGLDEAGFYDIPIMSYAVKYASAFYGPFRDAAGSAPQFGDRKSYQMDPANREEALREAMSDEQE
GADFLIVKPSLSYLDIMRDVKNNTNLPVVAYNVSGEYAMVKAAAQNGWIDEEKIVLEMLTSMKRAGATLIITYFAKDASK
YLNK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=322, Percent_Identity=44.0993788819876, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=52.3659305993691, Blast_Score=327, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6321398, Length=323, Percent_Identity=42.7244582043344, Blast_Score=257, Evalue=1e-69,
Organism=Drosophila melanogaster, GI21358291, Length=288, Percent_Identity=38.8888888888889, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36203; Mature: 36203

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQFDRHRRLRKTKTMRDLVRETVLHTDDLIYPIFVKDGKEPKTEVASMPGVFQYPLHE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHH
LEEEMRVVESLGIKAVILFGIPAEKDAVGTQAYHDHGIIQEATRLIKKSFPEILVVADTC
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEHHHH
LCEFTDHGHCGVIENGEILNDESLELLKQTAVSQAAAGADIIAPSNMMDGFVQVIREGLD
HHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EAGFYDIPIMSYAVKYASAFYGPFRDAAGSAPQFGDRKSYQMDPANREEALREAMSDEQE
HCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHC
GADFLIVKPSLSYLDIMRDVKNNTNLPVVAYNVSGEYAMVKAAAQNGWIDEEKIVLEMLT
CCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHHCCCCCHHHHHHHHHH
SMKRAGATLIITYFAKDASKYLNK
HHHHCCCEEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MKNQFDRHRRLRKTKTMRDLVRETVLHTDDLIYPIFVKDGKEPKTEVASMPGVFQYPLHE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHH
LEEEMRVVESLGIKAVILFGIPAEKDAVGTQAYHDHGIIQEATRLIKKSFPEILVVADTC
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEHHHH
LCEFTDHGHCGVIENGEILNDESLELLKQTAVSQAAAGADIIAPSNMMDGFVQVIREGLD
HHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EAGFYDIPIMSYAVKYASAFYGPFRDAAGSAPQFGDRKSYQMDPANREEALREAMSDEQE
HCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHC
GADFLIVKPSLSYLDIMRDVKNNTNLPVVAYNVSGEYAMVKAAAQNGWIDEEKIVLEMLT
CCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHHCCCCCHHHHHHHHHH
SMKRAGATLIITYFAKDASKYLNK
HHHHCCCEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]