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The map label for this gene is hemL

Identifier: 226224154

GI number: 226224154

Start: 1572874

End: 1574163

Strand: Reverse

Name: hemL

Synonym: Lm4b_01564

Alternate gene names: 226224154

Gene position: 1574163-1572874 (Counterclockwise)

Preceding gene: 226224155

Following gene: 226224153

Centisome position: 54.04

GC content: 40.39

Gene sequence:

>1290_bases
TTGCAAAACTATTCGAAATCCGAAAAGGCCTTTAAAGAAGCCAAAAAAGTCTTACCTGGTGGTGTAAATAGCCCTGTTCG
AGCATTCAACTCTGTAGATGCCTCGCCAGTTTTTATGGACCACGGAAAAGGCGCTTATATTACAGATGTTGATGGTAACG
AATATATTGATTATGTGTTATCTTGGGGCCCTCTAATTTTAGGGCACGCTGATCCAGCTGTTGTTAATGCTATTACAAAA
GCAGCTCTGAAAGGTACCAGCTTTGGGACGCCTACTGAGATTGAAACTGAATTAGCAAAATTAGTAATTGAACGTGTGCC
ATCTATTGAAATTGTTCGTATGGTTTCATCAGGAACGGAAGCAACGATGAGTGCTATTCGTTTGGCTCGCGGTTATACAA
AAAGAGAAAAAATCTTGAAATTTGAAGGAAGTTATCACGGACATGGAGATTCTTTATTAATTAAAGCTGGTTCAGGGGTA
GCAACACTCGGATTACCGGATTCTCCAGGAGTTACTAAAGGTCTTGCGGCAGATACAATAACAGTCCCATACAATGATAT
TGAAGGAGCCGAACTGGCCTTCCAAAAATATGGAGAAGAAATAGCAGCAGTCATAGTTGAACCAGTCGCGGGCAATATGG
GTGTTGTTCCTCCAATTGATGGATTTTTAGAAGGACTTCGAGAGCTTACTACAAAATTTGGCTCCTTGCTCATTTTTGAT
GAAGTTATGACCGGATTTAGAGTAGATTACTATTCTGCTCAAGGCTATTATGTGGTAACGCCCGATCTTACTTGTTTAGG
AAAAGTGATTGGTGGTGGTTTACCAGTTGGTGCTTATGGTGGAAAGAAAGAAATTATGGAACAGATTGCTCCTGCTGGTT
CTATTTATCAAGCGGGAACTTTATCCGGAAATCCATTAGCAATGAATGCTGGCTTTGAAACAGTTCGCCAACTAACGCCG
CAACACTATGATGTTTTCCGCACCCTAATTAAGCGAATGGAAGAAGGACTTACAGAGATATCTGCTAGAAGACAAGTCCC
GCTCTCGATTAATAAGGCTGGTTCGATGTTCGGCTTTTTCTTCACTGATCAAAAAGTGATTAATTTTGATACAGCAAAAA
CAAGTAATCTAGAATTTTTCCGAAATTATTACCGTGAAATGTTAGGTCAAGGCATTTTCTTACCACCGTCTCAGTTTGAG
GGTGTCTTCATTTCTACGATGCACACCGAGAAAGAAATTGATAAGACCTTGGAAGCATTTGATACGACATGCAAAATACT
TCGCGGCTAA

Upstream 100 bases:

>100_bases
AGAAATGCTAACAAGTATGAAACGAGCGGGTGCAACACTTATCATTACTTATTTTGCAAAAGATGCATCCAAATATTTAA
ATAAATAGGAGGTAAGAAAT

Downstream 100 bases:

>100_bases
TTGACGAATCTTGAAACTTTTGTTAAAATTATTTCTAGTATATAGAATCAAATGCAATGAAGAGGAAGAGTAGGACATTT
ATTTTTTTAAAGAGAGAAAG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1 [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQNYSKSEKAFKEAKKVLPGGVNSPVRAFNSVDASPVFMDHGKGAYITDVDGNEYIDYVLSWGPLILGHADPAVVNAITK
AALKGTSFGTPTEIETELAKLVIERVPSIEIVRMVSSGTEATMSAIRLARGYTKREKILKFEGSYHGHGDSLLIKAGSGV
ATLGLPDSPGVTKGLAADTITVPYNDIEGAELAFQKYGEEIAAVIVEPVAGNMGVVPPIDGFLEGLRELTTKFGSLLIFD
EVMTGFRVDYYSAQGYYVVTPDLTCLGKVIGGGLPVGAYGGKKEIMEQIAPAGSIYQAGTLSGNPLAMNAGFETVRQLTP
QHYDVFRTLIKRMEEGLTEISARRQVPLSINKAGSMFGFFFTDQKVINFDTAKTSNLEFFRNYYREMLGQGIFLPPSQFE
GVFISTMHTEKEIDKTLEAFDTTCKILRG

Sequences:

>Translated_429_residues
MQNYSKSEKAFKEAKKVLPGGVNSPVRAFNSVDASPVFMDHGKGAYITDVDGNEYIDYVLSWGPLILGHADPAVVNAITK
AALKGTSFGTPTEIETELAKLVIERVPSIEIVRMVSSGTEATMSAIRLARGYTKREKILKFEGSYHGHGDSLLIKAGSGV
ATLGLPDSPGVTKGLAADTITVPYNDIEGAELAFQKYGEEIAAVIVEPVAGNMGVVPPIDGFLEGLRELTTKFGSLLIFD
EVMTGFRVDYYSAQGYYVVTPDLTCLGKVIGGGLPVGAYGGKKEIMEQIAPAGSIYQAGTLSGNPLAMNAGFETVRQLTP
QHYDVFRTLIKRMEEGLTEISARRQVPLSINKAGSMFGFFFTDQKVINFDTAKTSNLEFFRNYYREMLGQGIFLPPSQFE
GVFISTMHTEKEIDKTLEAFDTTCKILRG
>Mature_429_residues
MQNYSKSEKAFKEAKKVLPGGVNSPVRAFNSVDASPVFMDHGKGAYITDVDGNEYIDYVLSWGPLILGHADPAVVNAITK
AALKGTSFGTPTEIETELAKLVIERVPSIEIVRMVSSGTEATMSAIRLARGYTKREKILKFEGSYHGHGDSLLIKAGSGV
ATLGLPDSPGVTKGLAADTITVPYNDIEGAELAFQKYGEEIAAVIVEPVAGNMGVVPPIDGFLEGLRELTTKFGSLLIFD
EVMTGFRVDYYSAQGYYVVTPDLTCLGKVIGGGLPVGAYGGKKEIMEQIAPAGSIYQAGTLSGNPLAMNAGFETVRQLTP
QHYDVFRTLIKRMEEGLTEISARRQVPLSINKAGSMFGFFFTDQKVINFDTAKTSNLEFFRNYYREMLGQGIFLPPSQFE
GVFISTMHTEKEIDKTLEAFDTTCKILRG

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=304, Percent_Identity=32.8947368421053, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI13994255, Length=314, Percent_Identity=27.3885350318471, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI37574042, Length=297, Percent_Identity=26.9360269360269, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI284507298, Length=219, Percent_Identity=33.7899543378995, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI24119277, Length=316, Percent_Identity=27.5316455696203, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI226442705, Length=301, Percent_Identity=26.2458471760797, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI226442709, Length=264, Percent_Identity=28.030303030303, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786349, Length=417, Percent_Identity=54.1966426858513, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789016, Length=347, Percent_Identity=29.971181556196, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789759, Length=332, Percent_Identity=31.9277108433735, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788044, Length=282, Percent_Identity=35.1063829787234, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693181, Length=361, Percent_Identity=29.6398891966759, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787560, Length=414, Percent_Identity=26.8115942028986, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786991, Length=295, Percent_Identity=29.1525423728814, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=295, Percent_Identity=29.1525423728814, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71992977, Length=392, Percent_Identity=26.0204081632653, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI32564660, Length=315, Percent_Identity=28.8888888888889, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144274, Length=140, Percent_Identity=36.4285714285714, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=310, Percent_Identity=30, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21357415, Length=300, Percent_Identity=30, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356575, Length=301, Percent_Identity=29.2358803986711, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46574; Mature: 46574

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNYSKSEKAFKEAKKVLPGGVNSPVRAFNSVDASPVFMDHGKGAYITDVDGNEYIDYVL
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHH
SWGPLILGHADPAVVNAITKAALKGTSFGTPTEIETELAKLVIERVPSIEIVRMVSSGTE
HCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHCCHH
ATMSAIRLARGYTKREKILKFEGSYHGHGDSLLIKAGSGVATLGLPDSPGVTKGLAADTI
HHHHHHHHHHCCHHHHHHEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEE
TVPYNDIEGAELAFQKYGEEIAAVIVEPVAGNMGVVPPIDGFLEGLRELTTKFGSLLIFD
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
EVMTGFRVDYYSAQGYYVVTPDLTCLGKVIGGGLPVGAYGGKKEIMEQIAPAGSIYQAGT
HHHHCCEEEEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMNAGFETVRQLTPQHYDVFRTLIKRMEEGLTEISARRQVPLSINKAGSMFGFF
CCCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEE
FTDQKVINFDTAKTSNLEFFRNYYREMLGQGIFLPPSQFEGVFISTMHTEKEIDKTLEAF
EECCEEEEECCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHH
DTTCKILRG
HHHHHHHCC
>Mature Secondary Structure
MQNYSKSEKAFKEAKKVLPGGVNSPVRAFNSVDASPVFMDHGKGAYITDVDGNEYIDYVL
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHH
SWGPLILGHADPAVVNAITKAALKGTSFGTPTEIETELAKLVIERVPSIEIVRMVSSGTE
HCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHCCHH
ATMSAIRLARGYTKREKILKFEGSYHGHGDSLLIKAGSGVATLGLPDSPGVTKGLAADTI
HHHHHHHHHHCCHHHHHHEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEE
TVPYNDIEGAELAFQKYGEEIAAVIVEPVAGNMGVVPPIDGFLEGLRELTTKFGSLLIFD
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
EVMTGFRVDYYSAQGYYVVTPDLTCLGKVIGGGLPVGAYGGKKEIMEQIAPAGSIYQAGT
HHHHCCEEEEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMNAGFETVRQLTPQHYDVFRTLIKRMEEGLTEISARRQVPLSINKAGSMFGFF
CCCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEE
FTDQKVINFDTAKTSNLEFFRNYYREMLGQGIFLPPSQFEGVFISTMHTEKEIDKTLEAF
EECCEEEEECCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHH
DTTCKILRG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]