Click here to switch to the map view.

The map label for this gene is queA

Identifier: 226224132

GI number: 226224132

Start: 1550064

End: 1551092

Strand: Reverse

Name: queA

Synonym: Lm4b_01541

Alternate gene names: 226224132

Gene position: 1551092-1550064 (Counterclockwise)

Preceding gene: 226224133

Following gene: 226224131

Centisome position: 53.25

GC content: 40.14

Gene sequence:

>1029_bases
ATGAAAGTAGAAGATTTCGATTTTGATTTACCAGAAGAATTAATCGCACAAACCCCACTATTAGACCGGACTTCAAGCCG
GCTAATGGTGCTTGATAAAAAGTCAGGTGACATAAAAGACCAACATTTCACTGATATTATCAGCTATTTAAACGAAGGGG
ATGCACTAGTATTAAATGACACACGTGTTCTCCCAGCAAGGCTGCATGGTATTAAAGATGAAACTGGCGCGCACATTGAA
GTGCTCCTTTTGAAACAAAAAGAAGGCAATGCATGGGAAACTTTAGTAAAACCTGCCAAAAGAATTCGAAAAGGTGCTAC
CATTACTTTTGGTGATGGTGCTTTAAAAGCGATTTGCTTGGAAGAGCTCGAGCATGGTGGTCGGATTTTAGAATTTTCTT
ATGAAGGCATTTTTTATGAAGTTTTAGAACAACTTGGCGAGATGCCACTTCCACCATACATTAAAGAACAACTTGCTGAT
CAGGATCGTTATCAAACCGTCTATGCGAAAGAGAATGGTTCAGCAGCAGCGCCGACAGCGGGCCTTCATTTTACAGAAGA
TTTACTGGCGAAAATTAGCGCAAAAGGTGTAGAAATCATTTTCGTGACGCTTCATGTAGGGCTCGGAACATTCCGCCCAG
TGGACGTAGAAGATACTGCCAATCATAAAATGCATTCTGAATTTTATCGTTTAACAGAAGAATCTGCTGAGCGAATTAAT
AAAATCAAAGCACAAGGCGGAAAAGTTGTTGCAGTTGGAACGACTTCTATCCGAACATTAGAAACAATTGCGAGTCGTCA
TGACGGCAAACTAGTGGCTGAATCAGGTTGGACAGAGATCTTCATTTCCCCAGGGTATACTTTTCAGGCAGTAGATGCAC
TAATAACGAATTTCCATTTACCAAAATCAACATTAATTATGCTCGTGTCTGCTTTATCGGACCGCACGAAAATTTTAGCA
GCCTATAATCATGCTGTAGAAGAACAATATCGCTTCTTTAGTTTTGGCGATGCCATGTTTATTCATTAA

Upstream 100 bases:

>100_bases
ACTACAAATCGGTTTTCTACAAAGGACGCCGCGCGGAAGAATCGCAACTGAAACGGCGTATAACCATTTAGGAATAAGTT
ATGAAAAAGAGGTATAAAGC

Downstream 100 bases:

>100_bases
ATTGCGAAAAAATTAATAGAAAAGCTTGTTTTCATTGGTAATTTTCGTTATGCTAGTGAATAGAGTAAATAAGAAAGAGG
TAACACATGTCTGCCATTCG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKVEDFDFDLPEELIAQTPLLDRTSSRLMVLDKKSGDIKDQHFTDIISYLNEGDALVLNDTRVLPARLHGIKDETGAHIE
VLLLKQKEGNAWETLVKPAKRIRKGATITFGDGALKAICLEELEHGGRILEFSYEGIFYEVLEQLGEMPLPPYIKEQLAD
QDRYQTVYAKENGSAAAPTAGLHFTEDLLAKISAKGVEIIFVTLHVGLGTFRPVDVEDTANHKMHSEFYRLTEESAERIN
KIKAQGGKVVAVGTTSIRTLETIASRHDGKLVAESGWTEIFISPGYTFQAVDALITNFHLPKSTLIMLVSALSDRTKILA
AYNHAVEEQYRFFSFGDAMFIH

Sequences:

>Translated_342_residues
MKVEDFDFDLPEELIAQTPLLDRTSSRLMVLDKKSGDIKDQHFTDIISYLNEGDALVLNDTRVLPARLHGIKDETGAHIE
VLLLKQKEGNAWETLVKPAKRIRKGATITFGDGALKAICLEELEHGGRILEFSYEGIFYEVLEQLGEMPLPPYIKEQLAD
QDRYQTVYAKENGSAAAPTAGLHFTEDLLAKISAKGVEIIFVTLHVGLGTFRPVDVEDTANHKMHSEFYRLTEESAERIN
KIKAQGGKVVAVGTTSIRTLETIASRHDGKLVAESGWTEIFISPGYTFQAVDALITNFHLPKSTLIMLVSALSDRTKILA
AYNHAVEEQYRFFSFGDAMFIH
>Mature_342_residues
MKVEDFDFDLPEELIAQTPLLDRTSSRLMVLDKKSGDIKDQHFTDIISYLNEGDALVLNDTRVLPARLHGIKDETGAHIE
VLLLKQKEGNAWETLVKPAKRIRKGATITFGDGALKAICLEELEHGGRILEFSYEGIFYEVLEQLGEMPLPPYIKEQLAD
QDRYQTVYAKENGSAAAPTAGLHFTEDLLAKISAKGVEIIFVTLHVGLGTFRPVDVEDTANHKMHSEFYRLTEESAERIN
KIKAQGGKVVAVGTTSIRTLETIASRHDGKLVAESGWTEIFISPGYTFQAVDALITNFHLPKSTLIMLVSALSDRTKILA
AYNHAVEEQYRFFSFGDAMFIH

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=47.8386167146974, Blast_Score=304, Evalue=5e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38183; Mature: 38183

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEDFDFDLPEELIAQTPLLDRTSSRLMVLDKKSGDIKDQHFTDIISYLNEGDALVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVLPARLHGIKDETGAHIEVLLLKQKEGNAWETLVKPAKRIRKGATITFGDGALKAICL
CEECHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
EELEHGGRILEFSYEGIFYEVLEQLGEMPLPPYIKEQLADQDRYQTVYAKENGSAAAPTA
HHHHCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHEEEEEECCCCCCCCCC
GLHFTEDLLAKISAKGVEIIFVTLHVGLGTFRPVDVEDTANHKMHSEFYRLTEESAERIN
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KIKAQGGKVVAVGTTSIRTLETIASRHDGKLVAESGWTEIFISPGYTFQAVDALITNFHL
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHCCC
PKSTLIMLVSALSDRTKILAAYNHAVEEQYRFFSFGDAMFIH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEC
>Mature Secondary Structure
MKVEDFDFDLPEELIAQTPLLDRTSSRLMVLDKKSGDIKDQHFTDIISYLNEGDALVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVLPARLHGIKDETGAHIEVLLLKQKEGNAWETLVKPAKRIRKGATITFGDGALKAICL
CEECHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
EELEHGGRILEFSYEGIFYEVLEQLGEMPLPPYIKEQLADQDRYQTVYAKENGSAAAPTA
HHHHCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHEEEEEECCCCCCCCCC
GLHFTEDLLAKISAKGVEIIFVTLHVGLGTFRPVDVEDTANHKMHSEFYRLTEESAERIN
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KIKAQGGKVVAVGTTSIRTLETIASRHDGKLVAESGWTEIFISPGYTFQAVDALITNFHL
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHCCC
PKSTLIMLVSALSDRTKILAAYNHAVEEQYRFFSFGDAMFIH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]