Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 226224133

GI number: 226224133

Start: 1551096

End: 1552103

Strand: Reverse

Name: ruvB

Synonym: Lm4b_01542

Alternate gene names: 226224133

Gene position: 1552103-1551096 (Counterclockwise)

Preceding gene: 226224134

Following gene: 226224132

Centisome position: 53.29

GC content: 40.87

Gene sequence:

>1008_bases
ATGGATGAACGAATTATTTCAAGTGAAACGGTAGACGCAGAAGAAGTATCTTTTGAAACTAGTTTGCGGCCACAGAACCT
TTCACAATATATTGGGCAAGACAAAGTAAAGAATAATTTAACAGTTTTTATAGAAGCTGCCACACTCCGGAATGAAGCCT
TGGATCACGTGCTTTTATACGGACCTCCAGGGCTTGGTAAAACAACGCTTGCAATGGTTATTGCAGCTGAAATGGGGAGT
CAAATTAAAACAACGAGTGGACCAGCAATTGAACGACCAGGTGACTTGGCGACAATTTTAACAAGCCTTGAGCCAGGGGA
TGTTTTATTTATTGATGAAATCCATCGTTTATCGAGAGCGATTGAAGAAATTTTATATCCGGCAATGGAAGATTATTGTT
TGGATATTGTGATTGGAACAGGTCCAACTGCGCGCTCTGTTCGTCTAGACTTACCACCATTTACATTGATTGGAGCTACC
ACACGAGCGGGACTTTTATCGGCACCACTCAGAGATCGTTTTGGCGTAATTGACCACTTGGAATTTTATACAGAAGAGCA
GTTAACAGAAATTGTACTCAGAACTTCGAATATATTAGATACAAAGATTGATGATCTTGGTGCACGTGAAATAGCTAGAC
GTTCAAGAGGAACCCCACGGATTGCCAATCGTTTATTAAAACGGGTACGTGATTTCGCGCAAGTTCGAGGAAACGGAACG
GTAACAGAAAAGCTAGCGAAAGAAGCACTTACTTTGCTTCAAGTAGATCCAAGAGGATTAGATACCATTGACCAAAAATT
GCTCCACACAATTATCCAATCATTTAGAGGCGGTCCAGTTGGACTGGATACAATCGCTGCAAGTATTGGAGAAGAACGAG
AAACCATTGAAGATATGCAAGAACCATATTTACTACAAATCGGTTTTCTACAAAGGACGCCGCGCGGAAGAATCGCAACT
GAAACGGCGTATAACCATTTAGGAATAAGTTATGAAAAAGAGGTATAA

Upstream 100 bases:

>100_bases
AAAAGTAATACCAAAATTGTCAAAAGAAGAAGACCTAACAAGCGACGCATATATCAAGCTAGCACTACAATTAATGACAA
AATAGAGAGGAGCTTGTGGC

Downstream 100 bases:

>100_bases
AGCATGAAAGTAGAAGATTTCGATTTTGATTTACCAGAAGAATTAATCGCACAAACCCCACTATTAGACCGGACTTCAAG
CCGGCTAATGGTGCTTGATA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MDERIISSETVDAEEVSFETSLRPQNLSQYIGQDKVKNNLTVFIEAATLRNEALDHVLLYGPPGLGKTTLAMVIAAEMGS
QIKTTSGPAIERPGDLATILTSLEPGDVLFIDEIHRLSRAIEEILYPAMEDYCLDIVIGTGPTARSVRLDLPPFTLIGAT
TRAGLLSAPLRDRFGVIDHLEFYTEEQLTEIVLRTSNILDTKIDDLGAREIARRSRGTPRIANRLLKRVRDFAQVRGNGT
VTEKLAKEALTLLQVDPRGLDTIDQKLLHTIIQSFRGGPVGLDTIAASIGEERETIEDMQEPYLLQIGFLQRTPRGRIAT
ETAYNHLGISYEKEV

Sequences:

>Translated_335_residues
MDERIISSETVDAEEVSFETSLRPQNLSQYIGQDKVKNNLTVFIEAATLRNEALDHVLLYGPPGLGKTTLAMVIAAEMGS
QIKTTSGPAIERPGDLATILTSLEPGDVLFIDEIHRLSRAIEEILYPAMEDYCLDIVIGTGPTARSVRLDLPPFTLIGAT
TRAGLLSAPLRDRFGVIDHLEFYTEEQLTEIVLRTSNILDTKIDDLGAREIARRSRGTPRIANRLLKRVRDFAQVRGNGT
VTEKLAKEALTLLQVDPRGLDTIDQKLLHTIIQSFRGGPVGLDTIAASIGEERETIEDMQEPYLLQIGFLQRTPRGRIAT
ETAYNHLGISYEKEV
>Mature_335_residues
MDERIISSETVDAEEVSFETSLRPQNLSQYIGQDKVKNNLTVFIEAATLRNEALDHVLLYGPPGLGKTTLAMVIAAEMGS
QIKTTSGPAIERPGDLATILTSLEPGDVLFIDEIHRLSRAIEEILYPAMEDYCLDIVIGTGPTARSVRLDLPPFTLIGAT
TRAGLLSAPLRDRFGVIDHLEFYTEEQLTEIVLRTSNILDTKIDDLGAREIARRSRGTPRIANRLLKRVRDFAQVRGNGT
VTEKLAKEALTLLQVDPRGLDTIDQKLLHTIIQSFRGGPVGLDTIAASIGEERETIEDMQEPYLLQIGFLQRTPRGRIAT
ETAYNHLGISYEKEV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=328, Percent_Identity=60.6707317073171, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1787119, Length=150, Percent_Identity=32.6666666666667, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37107; Mature: 37107

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERIISSETVDAEEVSFETSLRPQNLSQYIGQDKVKNNLTVFIEAATLRNEALDHVLLY
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHCCCEEEEEEEHHHHHHHHCEEEEE
GPPGLGKTTLAMVIAAEMGSQIKTTSGPAIERPGDLATILTSLEPGDVLFIDEIHRLSRA
CCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
IEEILYPAMEDYCLDIVIGTGPTARSVRLDLPPFTLIGATTRAGLLSAPLRDRFGVIDHL
HHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCCHHHHHHHCCHHHHCCHHHHH
EFYTEEQLTEIVLRTSNILDTKIDDLGAREIARRSRGTPRIANRLLKRVRDFAQVRGNGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCH
VTEKLAKEALTLLQVDPRGLDTIDQKLLHTIIQSFRGGPVGLDTIAASIGEERETIEDMQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHC
EPYLLQIGFLQRTPRGRIATETAYNHLGISYEKEV
CCEEEEEHHHHCCCCCCEEHHHHHHHCCCEECCCC
>Mature Secondary Structure
MDERIISSETVDAEEVSFETSLRPQNLSQYIGQDKVKNNLTVFIEAATLRNEALDHVLLY
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHCCCEEEEEEEHHHHHHHHCEEEEE
GPPGLGKTTLAMVIAAEMGSQIKTTSGPAIERPGDLATILTSLEPGDVLFIDEIHRLSRA
CCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
IEEILYPAMEDYCLDIVIGTGPTARSVRLDLPPFTLIGATTRAGLLSAPLRDRFGVIDHL
HHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCCHHHHHHHCCHHHHCCHHHHH
EFYTEEQLTEIVLRTSNILDTKIDDLGAREIARRSRGTPRIANRLLKRVRDFAQVRGNGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCH
VTEKLAKEALTLLQVDPRGLDTIDQKLLHTIIQSFRGGPVGLDTIAASIGEERETIEDMQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHC
EPYLLQIGFLQRTPRGRIATETAYNHLGISYEKEV
CCEEEEEHHHHCCCCCCEEHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]