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The map label for this gene is tgt

Identifier: 226224131

GI number: 226224131

Start: 1548838

End: 1549977

Strand: Reverse

Name: tgt

Synonym: Lm4b_01540

Alternate gene names: 226224131

Gene position: 1549977-1548838 (Counterclockwise)

Preceding gene: 226224132

Following gene: 226224130

Centisome position: 53.21

GC content: 40.0

Gene sequence:

>1140_bases
ATGTCTGCCATTCGTTATGAACTAATTAAAACAGATAAACAAACAGGTGCTCGTCTTGGTAAAATCCATACACCGCACGG
CACATTTGATACACCTATGTTTATGCCAGTTGGCACGCTTGCGACGGTAAAAACAATGTCACCCGAAGAATTAAAAGCCA
TGGGTGCAGGTATTATTTTAAGCAATACGTATCATTTATGGTTGCGTCCTGGAGAAGAACTTATCCGAGAAGCGGGCGGA
CTACATAAATTTATGAACTGGGATCAACCAATCTTAACGGATTCAGGTGGTTTTCAGGTGTTTAGTTTGAGTAAAATGCG
TGATATCAAAGAAGAAGGCGTTCATTTCCGTAACCATTTAAACGGAGACAAACTTTTCCTATCACCAGAAAAAGCGATTC
AAATTCAAAATGCGCTTGGATCTGACATCATGATGAGTTTTGACGAATGTCCACCATATCCAGCGTCGCATGAATATATG
AAGAAATCAGTAGAAAGAACATCTCGCTGGGCTGAACGTGGCTTAAAAGCGCACGTTAGACCAGAAGACCAAGGTTTGTT
CGGTATCGTCCAAGGTGGAGCTTACGAAGATTTACGTGCGCAAAGTGCGAAAGATCTGGTATCTCTAGATTTCCCTGGAT
ACTCAATTGGTGGATTGTCTGTTGGTGAACCAAAAGACGTGATGAATCGCGTTTTGGAACACACGACACCACTACTACCA
GCTAATAAACCACGTTATCTAATGGGAGTAGGTTCGCCTGACTCATTGATTGATGGCGTAATTCGAGGAGTGGACATGTT
TGATTGTGTTCTTCCCACACGTATTGCACGAAATGGTACTTGTATGACATCTAGTGGTCGCTTAGTTATTAAAAATGCTA
AGTTTACCCATGATTTCCGTCCAATTGATGAAAATTGTGACTGTTATACTTGTAAAAATTACTCACGTGCATACATTCGA
CACTTGATTCGTTGTGAGGAAACATTTGGAATTCGACTTACAACTTATCATAATCTTCATTTTCTGTTAAACTTAATGAA
GCAAGTTCGTGGCGCTATTATGGAAGATCGTCTTGCTGATTTTAGGGAAGAATTTTTTGAGCAATATGGATTCAATCGTC
CTGATGCAAAAAATTTCTAA

Upstream 100 bases:

>100_bases
TGTTTATTCATTAAATTGCGAAAAAATTAATAGAAAAGCTTGTTTTCATTGGTAATTTTCGTTATGCTAGTGAATAGAGT
AAATAAGAAAGAGGTAACAC

Downstream 100 bases:

>100_bases
ATATATATAAAGTATCGAAAGGAGTTGGAAAATATGGGCGGTATTGTAACATTTATACCAATTATCTTGATGATCGTTTT
GTTTTACTTCTTATTAATCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSAIRYELIKTDKQTGARLGKIHTPHGTFDTPMFMPVGTLATVKTMSPEELKAMGAGIILSNTYHLWLRPGEELIREAGG
LHKFMNWDQPILTDSGGFQVFSLSKMRDIKEEGVHFRNHLNGDKLFLSPEKAIQIQNALGSDIMMSFDECPPYPASHEYM
KKSVERTSRWAERGLKAHVRPEDQGLFGIVQGGAYEDLRAQSAKDLVSLDFPGYSIGGLSVGEPKDVMNRVLEHTTPLLP
ANKPRYLMGVGSPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSSGRLVIKNAKFTHDFRPIDENCDCYTCKNYSRAYIR
HLIRCEETFGIRLTTYHNLHFLLNLMKQVRGAIMEDRLADFREEFFEQYGFNRPDAKNF

Sequences:

>Translated_379_residues
MSAIRYELIKTDKQTGARLGKIHTPHGTFDTPMFMPVGTLATVKTMSPEELKAMGAGIILSNTYHLWLRPGEELIREAGG
LHKFMNWDQPILTDSGGFQVFSLSKMRDIKEEGVHFRNHLNGDKLFLSPEKAIQIQNALGSDIMMSFDECPPYPASHEYM
KKSVERTSRWAERGLKAHVRPEDQGLFGIVQGGAYEDLRAQSAKDLVSLDFPGYSIGGLSVGEPKDVMNRVLEHTTPLLP
ANKPRYLMGVGSPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSSGRLVIKNAKFTHDFRPIDENCDCYTCKNYSRAYIR
HLIRCEETFGIRLTTYHNLHFLLNLMKQVRGAIMEDRLADFREEFFEQYGFNRPDAKNF
>Mature_378_residues
SAIRYELIKTDKQTGARLGKIHTPHGTFDTPMFMPVGTLATVKTMSPEELKAMGAGIILSNTYHLWLRPGEELIREAGGL
HKFMNWDQPILTDSGGFQVFSLSKMRDIKEEGVHFRNHLNGDKLFLSPEKAIQIQNALGSDIMMSFDECPPYPASHEYMK
KSVERTSRWAERGLKAHVRPEDQGLFGIVQGGAYEDLRAQSAKDLVSLDFPGYSIGGLSVGEPKDVMNRVLEHTTPLLPA
NKPRYLMGVGSPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSSGRLVIKNAKFTHDFRPIDENCDCYTCKNYSRAYIRH
LIRCEETFGIRLTTYHNLHFLLNLMKQVRGAIMEDRLADFREEFFEQYGFNRPDAKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=46.7032967032967, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI13375872, Length=292, Percent_Identity=26.3698630136986, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=55.1075268817204, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17544680, Length=367, Percent_Identity=46.3215258855586, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI25146502, Length=236, Percent_Identity=26.6949152542373, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19920510, Length=366, Percent_Identity=43.1693989071038, Blast_Score=320, Evalue=9e-88,
Organism=Drosophila melanogaster, GI21355311, Length=268, Percent_Identity=24.2537313432836, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42922; Mature: 42791

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIRYELIKTDKQTGARLGKIHTPHGTFDTPMFMPVGTLATVKTMSPEELKAMGAGIIL
CCCHHHHHHHCCHHHHHHHCCEECCCCCCCCCCCCCCCCHHHEECCCHHHHHHHCCCEEE
SNTYHLWLRPGEELIREAGGLHKFMNWDQPILTDSGGFQVFSLSKMRDIKEEGVHFRNHL
ECCEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCHHHHCC
NGDKLFLSPEKAIQIQNALGSDIMMSFDECPPYPASHEYMKKSVERTSRWAERGLKAHVR
CCCEEEECHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCC
PEDQGLFGIVQGGAYEDLRAQSAKDLVSLDFPGYSIGGLSVGEPKDVMNRVLEHTTPLLP
CCCCCEEEEECCCCHHHHHHHHHHCEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCC
ANKPRYLMGVGSPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSSGRLVIKNAKFTHDFR
CCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCC
PIDENCDCYTCKNYSRAYIRHLIRCEETFGIRLTTYHNLHFLLNLMKQVRGAIMEDRLAD
CCCCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
FREEFFEQYGFNRPDAKNF
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAIRYELIKTDKQTGARLGKIHTPHGTFDTPMFMPVGTLATVKTMSPEELKAMGAGIIL
CCHHHHHHHCCHHHHHHHCCEECCCCCCCCCCCCCCCCHHHEECCCHHHHHHHCCCEEE
SNTYHLWLRPGEELIREAGGLHKFMNWDQPILTDSGGFQVFSLSKMRDIKEEGVHFRNHL
ECCEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCHHHHCC
NGDKLFLSPEKAIQIQNALGSDIMMSFDECPPYPASHEYMKKSVERTSRWAERGLKAHVR
CCCEEEECHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCC
PEDQGLFGIVQGGAYEDLRAQSAKDLVSLDFPGYSIGGLSVGEPKDVMNRVLEHTTPLLP
CCCCCEEEEECCCCHHHHHHHHHHCEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCC
ANKPRYLMGVGSPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSSGRLVIKNAKFTHDFR
CCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCC
PIDENCDCYTCKNYSRAYIRHLIRCEETFGIRLTTYHNLHFLLNLMKQVRGAIMEDRLAD
CCCCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
FREEFFEQYGFNRPDAKNF
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]