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The map label for this gene is apt

Identifier: 226224125

GI number: 226224125

Start: 1542268

End: 1542789

Strand: Reverse

Name: apt

Synonym: Lm4b_01534

Alternate gene names: 226224125

Gene position: 1542789-1542268 (Counterclockwise)

Preceding gene: 226224126

Following gene: 226224124

Centisome position: 52.97

GC content: 36.21

Gene sequence:

>522_bases
ATGGAAATTAAAGATTTACAAGATTATGTAGCGATTGTGAACGATTGGCCCAAAAAAGGGATTGTATTTAAAGACATCAC
ACCCCTTATGAATGACGGAGAAGCATATCGTTTTGCTACAGATAAAATTGTGGAATACGCAAAAGAATTAAAAATTGATA
TTATTGTTGGACCAGAAGCACGTGGCTTTATTATCGGCTGCCCAGTTGCTTATGCGTTAGGAATTGGTTTTGCGCCTGTT
CGTAAACCAGGAAAACTTCCTCGTGAAACAATCGAAATGGAATACGATTTAGAATATGGTACGAATAAATTATCTATGCA
TAGTGATGCGATTAAACCGGGGCAACGCGTTTTAATTACAGATGATTTACTTGCAACTGGTGGAACAATCGAAGCAACTA
TCAAACTTGTAGAAGAATTAGGCGGTATTGTTGCTGGTTGTGCTTTCTTAATCGAACTAAAAGAGCTAGAAGGACATAAA
AAATTAAATGGTTACGACCGTTTAATCCTTATGCAATTATAA

Upstream 100 bases:

>100_bases
ACAAGAAAAAAACTGTTGTACTCTAACTATACGGAACTTCATAGTTGGATGGAATCAATGATGGAAACATCAATTTACCC
GAATGCGGAGGAACTAGAAA

Downstream 100 bases:

>100_bases
AAATCAAACTACTCCTATTATTTTAGGGGTAGTTTTTTGATTAAAAAGCTGAAAACTGCCAGAAAATGTGAAAAATCAAG
GAAAAGTGCTTTCTAAACAA

Product: adenine phosphoribosyltransferase

Products: NA

Alternate protein names: APRT [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MEIKDLQDYVAIVNDWPKKGIVFKDITPLMNDGEAYRFATDKIVEYAKELKIDIIVGPEARGFIIGCPVAYALGIGFAPV
RKPGKLPRETIEMEYDLEYGTNKLSMHSDAIKPGQRVLITDDLLATGGTIEATIKLVEELGGIVAGCAFLIELKELEGHK
KLNGYDRLILMQL

Sequences:

>Translated_173_residues
MEIKDLQDYVAIVNDWPKKGIVFKDITPLMNDGEAYRFATDKIVEYAKELKIDIIVGPEARGFIIGCPVAYALGIGFAPV
RKPGKLPRETIEMEYDLEYGTNKLSMHSDAIKPGQRVLITDDLLATGGTIEATIKLVEELGGIVAGCAFLIELKELEGHK
KLNGYDRLILMQL
>Mature_173_residues
MEIKDLQDYVAIVNDWPKKGIVFKDITPLMNDGEAYRFATDKIVEYAKELKIDIIVGPEARGFIIGCPVAYALGIGFAPV
RKPGKLPRETIEMEYDLEYGTNKLSMHSDAIKPGQRVLITDDLLATGGTIEATIKLVEELGGIVAGCAFLIELKELEGHK
KLNGYDRLILMQL

Specific function: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis [H]

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4502171, Length=174, Percent_Identity=39.0804597701149, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI71773201, Length=129, Percent_Identity=40.3100775193798, Blast_Score=104, Evalue=4e-23,
Organism=Escherichia coli, GI1786675, Length=162, Percent_Identity=55.5555555555556, Blast_Score=186, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17509087, Length=159, Percent_Identity=42.7672955974843, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323619, Length=154, Percent_Identity=40.9090909090909, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320649, Length=166, Percent_Identity=33.1325301204819, Blast_Score=78, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17136334, Length=152, Percent_Identity=42.1052631578947, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005764
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.7 [H]

Molecular weight: Translated: 19183; Mature: 19183

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKDLQDYVAIVNDWPKKGIVFKDITPLMNDGEAYRFATDKIVEYAKELKIDIIVGPEA
CCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHCEEEEEECCCC
RGFIIGCPVAYALGIGFAPVRKPGKLPRETIEMEYDLEYGTNKLSMHSDAIKPGQRVLIT
CCEEEECHHHHHHHCCCCCCCCCCCCCHHHEEEEEECCCCCCEEEECCCCCCCCCEEEEE
DDLLATGGTIEATIKLVEELGGIVAGCAFLIELKELEGHKKLNGYDRLILMQL
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MEIKDLQDYVAIVNDWPKKGIVFKDITPLMNDGEAYRFATDKIVEYAKELKIDIIVGPEA
CCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHCEEEEEECCCC
RGFIIGCPVAYALGIGFAPVRKPGKLPRETIEMEYDLEYGTNKLSMHSDAIKPGQRVLIT
CCEEEECHHHHHHHCCCCCCCCCCCCCHHHEEEEEECCCCCCEEEECCCCCCCCCEEEEE
DDLLATGGTIEATIKLVEELGGIVAGCAFLIELKELEGHKKLNGYDRLILMQL
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]