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The map label for this gene is recJ [H]

Identifier: 226224126

GI number: 226224126

Start: 1542779

End: 1545130

Strand: Reverse

Name: recJ [H]

Synonym: Lm4b_01535

Alternate gene names: 226224126

Gene position: 1545130-1542779 (Counterclockwise)

Preceding gene: 226224127

Following gene: 226224125

Centisome position: 53.05

GC content: 38.44

Gene sequence:

>2352_bases
GTGATTCATTCAAAATACTTATGGAATATCGAGGAAGCGGCGGAAGACAAAGCAAGCCAATTAGCAGAACAGCTAAAAAT
TTCGCTTCCACTTGCAAAACTACTTTGGAAAAGAAAAATTACAACGCAAGGACAATTCGATAAATTTTTCCACCCAGAAA
AATATGAAAGCTATGACCCATTTTTATTTGCTGAAATGGATCTTGCGGTTGCTAGAATTAAGCGAGCGATAGAACTAAAT
GAGCAGATTTTAGTTTACGGAGATTATGATGCAGATGGAGTTACTAGTATTGCCGTTTTAATGAAAACACTGCGGCATTT
AGGAGCAAATGCTGAATTTTATATTCCTAATCGTTTTACGGAAGGTTATGGTCCAAATATCGCTGCTTTTGATATGGCTA
AAAACCAAGGAACGGATTTAATTATTACGGTTGATAATGGTATTGCGGCGCTTGAAGTAATGACGCATGCCAAAGAGATT
GGTTTAGATGTTATTGTGACGGATCATCATGAACCACGGGAAGTTATGCCGGAAGCTGTTGCAGTAATTCATCCGAAGCA
TCCTAAGTCAGCTTATCCGTTTGATGAATTAGCGGGTGTGGGTGTTGCTTATAAGTTATCGCATGCATTACTTGGAGAAG
AACCGAAAGAATTGCTTGATTTAGTTGCAGTTGGAACGGTGGCAGATTTAGTTTCTTTAACAGATGAAAATCGCTTACTT
GTCCAACTTGGATTGCGCCAATTGCGCGAATCCGCGAACCTTGGACTTTCAGTGTTAGCTAAAAAAGCTAGTTTGAAGTT
AGAAGAGGCTACGGAAGAAACTATTGGCTTTGGTTTAGCTCCACGACTGAATGCTGTTGGTCGTTTAGGCCCAGCAGATC
CAGCGGTGGATTTACTTTTAACAGAGGATCCAGAAGAAGCACTTTTCTTAGCAGAAGAAATTGATGATGCGAATAAAGAA
CGTAAACAAATAGTTGTTGATACAACGAAGCTCGCGATGGAGGCAATTGAGGCAAAAGAAACATTAGGAAATGTGCTCGT
TGTTTACGGAGAGGGTTGGAATACAGGGATTTTAGGGATAGTTGCTTCTAAATTAGTTGGAACATATTCTCGTCCGGCGA
TTGTACTAGGAATTGATCCAGTTACTGGAATTGCCAAAGGATCTGGTCGAAGCGTCGATGCATTTCATTTGTATCAAGCG
CTTGATAAACATCGTGATTTAATGACTGCATTTGGTGGGCATCCAATGGCTGCGGGTCTTACATTGCCCGCTGAAAATCT
AACAGAATTAGAAGCTAAATTACAAGAAGAAGCGAGTTTTCTTTCTGAAGAAGATTTCCGCCCAGCCTTGAAAATAGAAG
AGAAAATAAATATTGCGGATGTTTCCGTAGCATTTATTGCTCAACTTGAAAAATTAGCTCCTTTTGGAATGGATAACCCT
AAACCAATTTTCTTGTTAGAAAATATGCATTTAAAAGGAACGAAAAGAATTGGGGCAGATAAAACCCATTTAAAAACAAT
GCTTGCTACAGAGGAAAATGATGCCACTTTAGATTCTATCGGATTTGGTGTAGGCGATTTGGTAGAAAAAATTTCTCCAA
ATGCAGCCGTGGACGTGGTTGGGGAATTATCCATCAATGAATGGAATAATGTGAAAAAACCACAACTACGCATGATGGAC
ATTCACATCTCGCATTGGCAACTTTTCGATGTACGTAATAAATCTGAGTGGGCTAGGATTCTCCAAGAACAAACAGAATC
AAGAGTTTTTGTTTGTTTTGAAGAAAGAACGATTGGCACGCTTGGAGAAAAAAACTATATTTTAGTGGATGAAAAAGCTG
ATTTTACGGCGGATTTCCAGACAGAAGAACTTGTTTTTGCGGATATGCCAACGAAGACTGAATTAGTGGAAACGATTGTT
CGTGAAACGAAACCAGAGCGTATTTTTGTCCATTTTGATGCAGCAGAGGGGAATCAAATTCCAGCAATTCCAGACCGGTT
AGCCTTTGCTGAGCTATATAGTCTCATCAAAAAATTCCAACCATTTCCAATTGAAAAGTACACACCACGGCTAATGCAAA
AGTTTGGCTGGAATAAAGAGCAAATAGATTTCATGTCAAAGGTGTTTTTTGATTTAGAATTTGCTAAAATGGAAAGTGGT
CAGATTATTATTAATGAAGTTGTTGAAAAACGTAATCTGGACGAATCACTTGTTTATCAACAAAAGGTAGAAGAAATAAC
TACAAGAAAAAAACTGTTGTACTCTAACTATACGGAACTTCATAGTTGGATGGAATCAATGATGGAAACATCAATTTACC
CGAATGCGGAGGAACTAGAAAATGGAAATTAA

Upstream 100 bases:

>100_bases
TGGGACGCTGACAAAATATGTTGGCGTTTTTTGTTAGAAGATTCCTCTTGGTTTTTCTAGTGATTTTCGATATAATAGGA
AGGTTGAGAGGTGGGGTAAT

Downstream 100 bases:

>100_bases
AGATTTACAAGATTATGTAGCGATTGTGAACGATTGGCCCAAAAAAGGGATTGTATTTAAAGACATCACACCCCTTATGA
ATGACGGAGAAGCATATCGT

Product: single-stranded-DNA-specific exonuclease (RecJ)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 783; Mature: 783

Protein sequence:

>783_residues
MIHSKYLWNIEEAAEDKASQLAEQLKISLPLAKLLWKRKITTQGQFDKFFHPEKYESYDPFLFAEMDLAVARIKRAIELN
EQILVYGDYDADGVTSIAVLMKTLRHLGANAEFYIPNRFTEGYGPNIAAFDMAKNQGTDLIITVDNGIAALEVMTHAKEI
GLDVIVTDHHEPREVMPEAVAVIHPKHPKSAYPFDELAGVGVAYKLSHALLGEEPKELLDLVAVGTVADLVSLTDENRLL
VQLGLRQLRESANLGLSVLAKKASLKLEEATEETIGFGLAPRLNAVGRLGPADPAVDLLLTEDPEEALFLAEEIDDANKE
RKQIVVDTTKLAMEAIEAKETLGNVLVVYGEGWNTGILGIVASKLVGTYSRPAIVLGIDPVTGIAKGSGRSVDAFHLYQA
LDKHRDLMTAFGGHPMAAGLTLPAENLTELEAKLQEEASFLSEEDFRPALKIEEKINIADVSVAFIAQLEKLAPFGMDNP
KPIFLLENMHLKGTKRIGADKTHLKTMLATEENDATLDSIGFGVGDLVEKISPNAAVDVVGELSINEWNNVKKPQLRMMD
IHISHWQLFDVRNKSEWARILQEQTESRVFVCFEERTIGTLGEKNYILVDEKADFTADFQTEELVFADMPTKTELVETIV
RETKPERIFVHFDAAEGNQIPAIPDRLAFAELYSLIKKFQPFPIEKYTPRLMQKFGWNKEQIDFMSKVFFDLEFAKMESG
QIIINEVVEKRNLDESLVYQQKVEEITTRKKLLYSNYTELHSWMESMMETSIYPNAEELENGN

Sequences:

>Translated_783_residues
MIHSKYLWNIEEAAEDKASQLAEQLKISLPLAKLLWKRKITTQGQFDKFFHPEKYESYDPFLFAEMDLAVARIKRAIELN
EQILVYGDYDADGVTSIAVLMKTLRHLGANAEFYIPNRFTEGYGPNIAAFDMAKNQGTDLIITVDNGIAALEVMTHAKEI
GLDVIVTDHHEPREVMPEAVAVIHPKHPKSAYPFDELAGVGVAYKLSHALLGEEPKELLDLVAVGTVADLVSLTDENRLL
VQLGLRQLRESANLGLSVLAKKASLKLEEATEETIGFGLAPRLNAVGRLGPADPAVDLLLTEDPEEALFLAEEIDDANKE
RKQIVVDTTKLAMEAIEAKETLGNVLVVYGEGWNTGILGIVASKLVGTYSRPAIVLGIDPVTGIAKGSGRSVDAFHLYQA
LDKHRDLMTAFGGHPMAAGLTLPAENLTELEAKLQEEASFLSEEDFRPALKIEEKINIADVSVAFIAQLEKLAPFGMDNP
KPIFLLENMHLKGTKRIGADKTHLKTMLATEENDATLDSIGFGVGDLVEKISPNAAVDVVGELSINEWNNVKKPQLRMMD
IHISHWQLFDVRNKSEWARILQEQTESRVFVCFEERTIGTLGEKNYILVDEKADFTADFQTEELVFADMPTKTELVETIV
RETKPERIFVHFDAAEGNQIPAIPDRLAFAELYSLIKKFQPFPIEKYTPRLMQKFGWNKEQIDFMSKVFFDLEFAKMESG
QIIINEVVEKRNLDESLVYQQKVEEITTRKKLLYSNYTELHSWMESMMETSIYPNAEELENGN
>Mature_783_residues
MIHSKYLWNIEEAAEDKASQLAEQLKISLPLAKLLWKRKITTQGQFDKFFHPEKYESYDPFLFAEMDLAVARIKRAIELN
EQILVYGDYDADGVTSIAVLMKTLRHLGANAEFYIPNRFTEGYGPNIAAFDMAKNQGTDLIITVDNGIAALEVMTHAKEI
GLDVIVTDHHEPREVMPEAVAVIHPKHPKSAYPFDELAGVGVAYKLSHALLGEEPKELLDLVAVGTVADLVSLTDENRLL
VQLGLRQLRESANLGLSVLAKKASLKLEEATEETIGFGLAPRLNAVGRLGPADPAVDLLLTEDPEEALFLAEEIDDANKE
RKQIVVDTTKLAMEAIEAKETLGNVLVVYGEGWNTGILGIVASKLVGTYSRPAIVLGIDPVTGIAKGSGRSVDAFHLYQA
LDKHRDLMTAFGGHPMAAGLTLPAENLTELEAKLQEEASFLSEEDFRPALKIEEKINIADVSVAFIAQLEKLAPFGMDNP
KPIFLLENMHLKGTKRIGADKTHLKTMLATEENDATLDSIGFGVGDLVEKISPNAAVDVVGELSINEWNNVKKPQLRMMD
IHISHWQLFDVRNKSEWARILQEQTESRVFVCFEERTIGTLGEKNYILVDEKADFTADFQTEELVFADMPTKTELVETIV
RETKPERIFVHFDAAEGNQIPAIPDRLAFAELYSLIKKFQPFPIEKYTPRLMQKFGWNKEQIDFMSKVFFDLEFAKMESG
QIIINEVVEKRNLDESLVYQQKVEEITTRKKLLYSNYTELHSWMESMMETSIYPNAEELENGN

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=519, Percent_Identity=34.6820809248555, Blast_Score=254, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 87752; Mature: 87752

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHSKYLWNIEEAAEDKASQLAEQLKISLPLAKLLWKRKITTQGQFDKFFHPEKYESYDP
CCCCCEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCCC
FLFAEMDLAVARIKRAIELNEQILVYGDYDADGVTSIAVLMKTLRHLGANAEFYIPNRFT
EEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
EGYGPNIAAFDMAKNQGTDLIITVDNGIAALEVMTHAKEIGLDVIVTDHHEPREVMPEAV
CCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
AVIHPKHPKSAYPFDELAGVGVAYKLSHALLGEEPKELLDLVAVGTVADLVSLTDENRLL
EEECCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VQLGLRQLRESANLGLSVLAKKASLKLEEATEETIGFGLAPRLNAVGRLGPADPAVDLLL
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEE
TEDPEEALFLAEEIDDANKERKQIVVDTTKLAMEAIEAKETLGNVLVVYGEGWNTGILGI
ECCCHHHHHHHHHHCCCCHHHCEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
VASKLVGTYSRPAIVLGIDPVTGIAKGSGRSVDAFHLYQALDKHRDLMTAFGGHPMAAGL
HHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCE
TLPAENLTELEAKLQEEASFLSEEDFRPALKIEEKINIADVSVAFIAQLEKLAPFGMDNP
ECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
KPIFLLENMHLKGTKRIGADKTHLKTMLATEENDATLDSIGFGVGDLVEKISPNAAVDVV
CCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCCCCEEEE
GELSINEWNNVKKPQLRMMDIHISHWQLFDVRNKSEWARILQEQTESRVFVCFEERTIGT
EEEECCCCCCCCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCC
LGEKNYILVDEKADFTADFQTEELVFADMPTKTELVETIVRETKPERIFVHFDAAEGNQI
CCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PAIPDRLAFAELYSLIKKFQPFPIEKYTPRLMQKFGWNKEQIDFMSKVFFDLEFAKMESG
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
QIIINEVVEKRNLDESLVYQQKVEEITTRKKLLYSNYTELHSWMESMMETSIYPNAEELE
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCC
NGN
CCC
>Mature Secondary Structure
MIHSKYLWNIEEAAEDKASQLAEQLKISLPLAKLLWKRKITTQGQFDKFFHPEKYESYDP
CCCCCEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCCC
FLFAEMDLAVARIKRAIELNEQILVYGDYDADGVTSIAVLMKTLRHLGANAEFYIPNRFT
EEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
EGYGPNIAAFDMAKNQGTDLIITVDNGIAALEVMTHAKEIGLDVIVTDHHEPREVMPEAV
CCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
AVIHPKHPKSAYPFDELAGVGVAYKLSHALLGEEPKELLDLVAVGTVADLVSLTDENRLL
EEECCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VQLGLRQLRESANLGLSVLAKKASLKLEEATEETIGFGLAPRLNAVGRLGPADPAVDLLL
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEE
TEDPEEALFLAEEIDDANKERKQIVVDTTKLAMEAIEAKETLGNVLVVYGEGWNTGILGI
ECCCHHHHHHHHHHCCCCHHHCEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
VASKLVGTYSRPAIVLGIDPVTGIAKGSGRSVDAFHLYQALDKHRDLMTAFGGHPMAAGL
HHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCE
TLPAENLTELEAKLQEEASFLSEEDFRPALKIEEKINIADVSVAFIAQLEKLAPFGMDNP
ECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
KPIFLLENMHLKGTKRIGADKTHLKTMLATEENDATLDSIGFGVGDLVEKISPNAAVDVV
CCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCCCCEEEE
GELSINEWNNVKKPQLRMMDIHISHWQLFDVRNKSEWARILQEQTESRVFVCFEERTIGT
EEEECCCCCCCCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCC
LGEKNYILVDEKADFTADFQTEELVFADMPTKTELVETIVRETKPERIFVHFDAAEGNQI
CCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PAIPDRLAFAELYSLIKKFQPFPIEKYTPRLMQKFGWNKEQIDFMSKVFFDLEFAKMESG
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
QIIINEVVEKRNLDESLVYQQKVEEITTRKKLLYSNYTELHSWMESMMETSIYPNAEELE
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCC
NGN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]