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The map label for this gene is relA

Identifier: 226224124

GI number: 226224124

Start: 1539846

End: 1542062

Strand: Reverse

Name: relA

Synonym: Lm4b_01533

Alternate gene names: 226224124

Gene position: 1542062-1539846 (Counterclockwise)

Preceding gene: 226224125

Following gene: 226224123

Centisome position: 52.94

GC content: 37.8

Gene sequence:

>2217_bases
ATGGCGAAAGAACAAAATCTGACAGCTGAGCAGGTCATCGATATGGCTTCTCATTATATGAATCAGGAACATCTAGCGCT
CGTAAAAAAAGCGTATGAATTTGCGCGCGATTCTCATAAAGAGCAATTTCGTAAATCAGGTGAGCCGTATATTATTCATC
CAATTCAAGTTGCCGGCATTTTAGTCGAATTAAAAATGGATCCATCGACCGTTGCATCTGGATTTTTACATGATGTCGTG
GAAGATACGCCAGTCACACTAGCGGATTTAGAAGAAGTTTTTGGCAGTGAAGTTGCCATGCTAGTAGACGGAGTAACGAA
ACTTGGTAAAATTAAATATAAATCACACGAAGAACAACAAGCTGAAAATCATCGGAAAATGTTCATTGCGATGGCGCAGG
ATATTCGCGTTATTTTAATCAAACTGGCGGACCGTTTGCATAATATGCGGACATTAAAACATTTGCCCGTGGAAAAACAA
CGTAGAATTGCGAACGAAACATTAGAAATTTTTGCGCCACTTGCACATCGCTTGGGTATTTCGCGAGTGAAATGGGAGCT
GGAAGATACAGCGCTGCGTTATTTAAATCCACAACAATATTATCGCATCGTCCACTTAATGAAACAAAAGCGGGATGCAA
GAGAGCGTTATTTGCATGATGTGATTGACGGTGTGAATGAAAATCTAGATGAACTCAATATTCAAGCGGATATTTCTGGA
AGACCAAAACATATTTATTCGATTTACCGGAAAATGAGCGAACAAAATAAACAATTTAACGAAATTTATGATTTGTTGGC
TGTTCGAATTGTCGTTAGTAGTATCAAGGATTGCTATGCTGTTCTTGGTATTATTCATACGCGCTGGAAACCGATGCCAG
GTCGCTTTAAAGATTATATTGCGATGCCGAAGTCGAATATGTATCAATCGATTCACACGACGGTTATAGGGCCTCAAGGA
GAACCGCTTGAAGTTCAAATTAGAACGCACGAAATGCACCAAATCGCTGAATACGGGGTTGCGGCACACTGGGCCTATAA
AGAAGGCAAAGTGGTTAATTCCAAAACTTCATTCGATAATAAATTAACATGGTTCCGAGAAATTTTAGAATATCAAAATG
AATCGGATAATGCGGAAGAGTTTATGGAAAGCTTGAAACTCGACTTATTTTCGGATGTTGTTTACGTATTTACTCCAAAA
GGCGATGTGTACGAGTTACCAAATGGCTCTGTACCACTCGATTTTGCTTACCGTGTCCATACCGAAATCGGAAACAAAAC
AATCGGGGCTAAAATTAACGGAAAAATTGTGACACTCGACTATAAATTAAAAACGGGCGATATTATTGATATTTTAACGT
CCAAACATTCTTACGGCCCAAGTCGCGACTGGCTTAAATTAGTTCAAACTTCGCAAGCGCGAAATAAAATTAAACAATTT
TTCAAACGACAAGCAAAAGAAGAGAATGTCGAAAAAGGTCGCGATTTAGTTGAGAAAGAAATTAGACAACTTGGATTCGA
ATCGAAAAAAATTATGACGCCAGAAAATCTTCGTAAATTAGCTGATAAACTAAATTTTTCGCATGAAGATGATTTGTTTG
CAGCTGTTGGTTATAATGGCATTACAGCTTTACAAGTAGCTAACCGCTTAACAGAAAAACTTCGAAAAGAACGTGAATTA
GAAGCAGAAACAGAGAAGTTATTGACGCAATCAGAAAATAAACCTTCGAATTCAGATGCTAACAATGAGAAACTGAAGAT
TAAGCATAATGCAGGAGTAGTTGTTCAAGGAGTAGGTAATCTATTGATTCGACTATCCAGATGTTGTAATCCAGTGCCTG
GTGATGCTATCGTTGGTTATATTACAAAAGGTCGAGGCATTTCTATACATCGTCAAGATTGCCCAAATGTTCAAGCTATT
GAACCAGAGCGGCTGATCGAAGTGGACTGGGAAGATGCTGATTCACAAGCGAAAAATGACTATAATGTAGACATCGAAAT
TTATGGCTATAATCGTAACGGTTTGCTAAATGATATTCTACAAGTGATTAACAGTCTAACTTCCAATATTAACGGCGTCA
ATGCTAAGGTTGATAACAACAAGATGGCAACATTAGTGGTCACGCTACAGATTCATAATATTAATCATTTACAACGTGTA
GTGGATAAAATAAAACAAATTCCAGATGTTTATACAGTGAGAAGATTAATGAACTAA

Upstream 100 bases:

>100_bases
GCTCATGCTATAATATTAAACAAGAATATTTTTTAGAACACTCAGTTTTTGGGTGTTTTTAAGTTGTATATATGACATGG
AGATAAGAGGGTGAATGTAA

Downstream 100 bases:

>100_bases
AGGAGTGGAGAAGCGATGCGTGTACTACTGCAACGGTGTTATGAAGCTTCCGTGAGTGTGGAGGAGGAAGTCATTAGCGA
AATAGCTGGAGGTCTTTGTT

Product: (p)ppGpp synthetase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MAKEQNLTAEQVIDMASHYMNQEHLALVKKAYEFARDSHKEQFRKSGEPYIIHPIQVAGILVELKMDPSTVASGFLHDVV
EDTPVTLADLEEVFGSEVAMLVDGVTKLGKIKYKSHEEQQAENHRKMFIAMAQDIRVILIKLADRLHNMRTLKHLPVEKQ
RRIANETLEIFAPLAHRLGISRVKWELEDTALRYLNPQQYYRIVHLMKQKRDARERYLHDVIDGVNENLDELNIQADISG
RPKHIYSIYRKMSEQNKQFNEIYDLLAVRIVVSSIKDCYAVLGIIHTRWKPMPGRFKDYIAMPKSNMYQSIHTTVIGPQG
EPLEVQIRTHEMHQIAEYGVAAHWAYKEGKVVNSKTSFDNKLTWFREILEYQNESDNAEEFMESLKLDLFSDVVYVFTPK
GDVYELPNGSVPLDFAYRVHTEIGNKTIGAKINGKIVTLDYKLKTGDIIDILTSKHSYGPSRDWLKLVQTSQARNKIKQF
FKRQAKEENVEKGRDLVEKEIRQLGFESKKIMTPENLRKLADKLNFSHEDDLFAAVGYNGITALQVANRLTEKLRKEREL
EAETEKLLTQSENKPSNSDANNEKLKIKHNAGVVVQGVGNLLIRLSRCCNPVPGDAIVGYITKGRGISIHRQDCPNVQAI
EPERLIEVDWEDADSQAKNDYNVDIEIYGYNRNGLLNDILQVINSLTSNINGVNAKVDNNKMATLVVTLQIHNINHLQRV
VDKIKQIPDVYTVRRLMN

Sequences:

>Translated_738_residues
MAKEQNLTAEQVIDMASHYMNQEHLALVKKAYEFARDSHKEQFRKSGEPYIIHPIQVAGILVELKMDPSTVASGFLHDVV
EDTPVTLADLEEVFGSEVAMLVDGVTKLGKIKYKSHEEQQAENHRKMFIAMAQDIRVILIKLADRLHNMRTLKHLPVEKQ
RRIANETLEIFAPLAHRLGISRVKWELEDTALRYLNPQQYYRIVHLMKQKRDARERYLHDVIDGVNENLDELNIQADISG
RPKHIYSIYRKMSEQNKQFNEIYDLLAVRIVVSSIKDCYAVLGIIHTRWKPMPGRFKDYIAMPKSNMYQSIHTTVIGPQG
EPLEVQIRTHEMHQIAEYGVAAHWAYKEGKVVNSKTSFDNKLTWFREILEYQNESDNAEEFMESLKLDLFSDVVYVFTPK
GDVYELPNGSVPLDFAYRVHTEIGNKTIGAKINGKIVTLDYKLKTGDIIDILTSKHSYGPSRDWLKLVQTSQARNKIKQF
FKRQAKEENVEKGRDLVEKEIRQLGFESKKIMTPENLRKLADKLNFSHEDDLFAAVGYNGITALQVANRLTEKLRKEREL
EAETEKLLTQSENKPSNSDANNEKLKIKHNAGVVVQGVGNLLIRLSRCCNPVPGDAIVGYITKGRGISIHRQDCPNVQAI
EPERLIEVDWEDADSQAKNDYNVDIEIYGYNRNGLLNDILQVINSLTSNINGVNAKVDNNKMATLVVTLQIHNINHLQRV
VDKIKQIPDVYTVRRLMN
>Mature_737_residues
AKEQNLTAEQVIDMASHYMNQEHLALVKKAYEFARDSHKEQFRKSGEPYIIHPIQVAGILVELKMDPSTVASGFLHDVVE
DTPVTLADLEEVFGSEVAMLVDGVTKLGKIKYKSHEEQQAENHRKMFIAMAQDIRVILIKLADRLHNMRTLKHLPVEKQR
RIANETLEIFAPLAHRLGISRVKWELEDTALRYLNPQQYYRIVHLMKQKRDARERYLHDVIDGVNENLDELNIQADISGR
PKHIYSIYRKMSEQNKQFNEIYDLLAVRIVVSSIKDCYAVLGIIHTRWKPMPGRFKDYIAMPKSNMYQSIHTTVIGPQGE
PLEVQIRTHEMHQIAEYGVAAHWAYKEGKVVNSKTSFDNKLTWFREILEYQNESDNAEEFMESLKLDLFSDVVYVFTPKG
DVYELPNGSVPLDFAYRVHTEIGNKTIGAKINGKIVTLDYKLKTGDIIDILTSKHSYGPSRDWLKLVQTSQARNKIKQFF
KRQAKEENVEKGRDLVEKEIRQLGFESKKIMTPENLRKLADKLNFSHEDDLFAAVGYNGITALQVANRLTEKLRKERELE
AETEKLLTQSENKPSNSDANNEKLKIKHNAGVVVQGVGNLLIRLSRCCNPVPGDAIVGYITKGRGISIHRQDCPNVQAIE
PERLIEVDWEDADSQAKNDYNVDIEIYGYNRNGLLNDILQVINSLTSNINGVNAKVDNNKMATLVVTLQIHNINHLQRVV
DKIKQIPDVYTVRRLMN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=130, Percent_Identity=38.4615384615385, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1790082, Length=724, Percent_Identity=40.8839779005525, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1789147, Length=699, Percent_Identity=39.9141630901288, Blast_Score=543, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25143118, Length=144, Percent_Identity=38.1944444444444, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650996, Length=128, Percent_Identity=36.71875, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 84717; Mature: 84586

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEQNLTAEQVIDMASHYMNQEHLALVKKAYEFARDSHKEQFRKSGEPYIIHPIQVAGI
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
LVELKMDPSTVASGFLHDVVEDTPVTLADLEEVFGSEVAMLVDGVTKLGKIKYKSHEEQQ
EEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHH
AENHRKMFIAMAQDIRVILIKLADRLHNMRTLKHLPVEKQRRIANETLEIFAPLAHRLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCH
SRVKWELEDTALRYLNPQQYYRIVHLMKQKRDARERYLHDVIDGVNENLDELNIQADISG
HHHEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEECCCC
RPKHIYSIYRKMSEQNKQFNEIYDLLAVRIVVSSIKDCYAVLGIIHTRWKPMPGRFKDYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AMPKSNMYQSIHTTVIGPQGEPLEVQIRTHEMHQIAEYGVAAHWAYKEGKVVNSKTSFDN
CCCCHHHHHHHHHEEECCCCCCEEEEEEHHHHHHHHHHCCCEEEECCCCCEECCCCCCCH
KLTWFREILEYQNESDNAEEFMESLKLDLFSDVVYVFTPKGDVYELPNGSVPLDFAYRVH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCEEEHEEEE
TEIGNKTIGAKINGKIVTLDYKLKTGDIIDILTSKHSYGPSRDWLKLVQTSQARNKIKQF
HHCCCEEECEEECCEEEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
FKRQAKEENVEKGRDLVEKEIRQLGFESKKIMTPENLRKLADKLNFSHEDDLFAAVGYNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
ITALQVANRLTEKLRKERELEAETEKLLTQSENKPSNSDANNEKLKIKHNAGVVVQGVGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEHHHH
LLIRLSRCCNPVPGDAIVGYITKGRGISIHRQDCPNVQAIEPERLIEVDWEDADSQAKND
HHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCC
YNVDIEIYGYNRNGLLNDILQVINSLTSNINGVNAKVDNNKMATLVVTLQIHNINHLQRV
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEEECCHHHHHHH
VDKIKQIPDVYTVRRLMN
HHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
AKEQNLTAEQVIDMASHYMNQEHLALVKKAYEFARDSHKEQFRKSGEPYIIHPIQVAGI
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
LVELKMDPSTVASGFLHDVVEDTPVTLADLEEVFGSEVAMLVDGVTKLGKIKYKSHEEQQ
EEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHH
AENHRKMFIAMAQDIRVILIKLADRLHNMRTLKHLPVEKQRRIANETLEIFAPLAHRLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCH
SRVKWELEDTALRYLNPQQYYRIVHLMKQKRDARERYLHDVIDGVNENLDELNIQADISG
HHHEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEECCCC
RPKHIYSIYRKMSEQNKQFNEIYDLLAVRIVVSSIKDCYAVLGIIHTRWKPMPGRFKDYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AMPKSNMYQSIHTTVIGPQGEPLEVQIRTHEMHQIAEYGVAAHWAYKEGKVVNSKTSFDN
CCCCHHHHHHHHHEEECCCCCCEEEEEEHHHHHHHHHHCCCEEEECCCCCEECCCCCCCH
KLTWFREILEYQNESDNAEEFMESLKLDLFSDVVYVFTPKGDVYELPNGSVPLDFAYRVH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCEEEHEEEE
TEIGNKTIGAKINGKIVTLDYKLKTGDIIDILTSKHSYGPSRDWLKLVQTSQARNKIKQF
HHCCCEEECEEECCEEEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
FKRQAKEENVEKGRDLVEKEIRQLGFESKKIMTPENLRKLADKLNFSHEDDLFAAVGYNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
ITALQVANRLTEKLRKERELEAETEKLLTQSENKPSNSDANNEKLKIKHNAGVVVQGVGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEHHHH
LLIRLSRCCNPVPGDAIVGYITKGRGISIHRQDCPNVQAIEPERLIEVDWEDADSQAKND
HHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCC
YNVDIEIYGYNRNGLLNDILQVINSLTSNINGVNAKVDNNKMATLVVTLQIHNINHLQRV
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEEECCHHHHHHH
VDKIKQIPDVYTVRRLMN
HHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]