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The map label for this gene is tktB

Identifier: 226223966

GI number: 226223966

Start: 1371088

End: 1372917

Strand: Direct

Name: tktB

Synonym: Lm4b_01374

Alternate gene names: 226223966

Gene position: 1371088-1372917 (Clockwise)

Preceding gene: 226223965

Following gene: 226223967

Centisome position: 47.07

GC content: 40.16

Gene sequence:

>1830_bases
GTGAGTTGTTTTTATTTGGATCTTTTAAAAATAAAGGATCCTTCCTTTATGAAGCAATTGGATATACAAGAATTAGAAGC
ACTTGCGGCAGATATTCGCGCTTTTTTAATTACTTCTACTTCTAAATCAGGTGGGCATATTGGTCCGAATCTTGGTGTGG
TAGAACTAACGATTGCGTTGCATTATTCTTTTAATAGTCCAAAAGATAAATTTATTTGGGATGTTGGTCACCAGTCATAT
GTGCACAAAATCCTAACTGGAAGGGCGAGCCAATTTGGTACTTTACGTGAGCACGGGGGATTAGATGGATTTCCAAAGCG
GAAAGAATCTATTCATGATGTATTTGAAACGGGGCACAGCTCAACTTCTTTGTCTGCTGCAGCCGGGATGGTTATTGCGA
GAGATATTAAAAAGGAAGAATTTTATGTTATTCCGATAATCGGTGATGGCGCCTTAACAGGTGGCATGGCTTTAGAGGCT
TTGAATCATATAGGCGATATGGGAAAAGATATGATAGTTATTTTAAATGATAATGATATGTCGATAGCGCCAAACGTGGG
AGCAATCCACAACATACTTGGAAAATTAAGGACCTCTGATACATTTAAGCAAACAAAAGCTAAAGTCGATGGTACTTTTT
TTGAAGAGCTTGGGTTTATGTATTTGGGCCCAATTAATGGGCATGATATAGAAGAGGTTATTACTAACTTGGAATTAGCA
AAACGCACAAAAGGGCCTGTACTGCTCCATATCGTTACAAAAAAAGGCAAAGGGTATCAACCGGCCGAATTAGACTCTCG
TGGCACCTGGCATGGTACAGGACCTTATAAAGTCGAAACAGGTAGCTTTATTAAGCCGGCTAAGCGTGCAGCGTCATGGA
GTTCAGTTATTAGCAATGAATTAATACGTTTGGCAGAAAAGGATGAACGGATTGTTGCGATAACTCCGGCAATGCCTGTT
GGCTCTAAATTAGAAAAATTTGCGAAAGCTTTCCCAGAACGCTTTTTTGATGTTGGGATTGCGGAACAGCATGCGACAAC
AATGGCAGCGGGGCTAGCAACACAAGGGATGAAACCGTTCCTTACTATTTATTCCACTTTTTTACAAAGAGCCTATGATC
AGTTAGTTCATGATGTTTGCCGGCAAAAGTTGAATGTGGTCATTGGGATTGACCGGGCCGGGCTTGTTGGCGCTGATGGC
GAAACACATCAAGGTATCTTTGATATTTCATTTTTAAATAGTATTCCAAACATGACTATTAGCATGCCGAAAGATGAAGT
GGAAGCTAGACAATTAATGGATACTGCGTTTTCGTATAATGATGGGCCCTTTGCGATTCGCTATCCACGCGGCGAAGCTC
CTGGTGCGCAGGTAGCTGAGTCTAATACGCTTATACCAATCGGAAAGTGGGAAACAATCATTCAGCCGATTGATGCTGTT
ATTTTAACCTTTGGCCCTACCATCCGGCTAGCGCTAAAAGCAGCTGAACAATTAGAGTTAGAAGGATACCACGTTGGAGT
AATTAATGCGCGCTATATTAAACCGTTAGACGAAGCACTTTTACACCAAATCTTAAAGCAAAAAATTCCCATTTTAACAG
TAGAAGAATCACTATTAAAAGGTGGATTTGGTGCGAGTGTGTTAGAATTTATAGAAGCGAGTAATTATAGCGATGTTGTA
ATGCATCGAATCGGTTTACCAGACGAATTTATTAGCCATGGTTCTGTATCCATAATTTTGGAATCATTTGGCATATCAAC
AACTGGAATAGTATTAAAAATAAAAGAAATGCTCGCACAAAGCGGGAAGTTAAGGGCGAAGAGACTATGA

Upstream 100 bases:

>100_bases
TTTTACCTTGTTTGGTTTAATGGTGATTGTTTGCTATAATAAGAACAATTAATTGAGAAAAAAGACCTTGCACACATTCA
TGCAAGTGGCTCTTTGGAAA

Downstream 100 bases:

>100_bases
CAATAAAAAAAGAACGCGCAGATATTCTGCTAGTAGAACAAGGACTATTTGAAACAAGAGAAAAGGCAAAACGTGCAATT
ATGGCGGGAATTGTTTATCG

Product: 1-deoxy-D-xylulose-5-phosphate synthase

Products: NA

Alternate protein names: 1-deoxyxylulose-5-phosphate synthase; DXP synthase; DXPS

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MSCFYLDLLKIKDPSFMKQLDIQELEALAADIRAFLITSTSKSGGHIGPNLGVVELTIALHYSFNSPKDKFIWDVGHQSY
VHKILTGRASQFGTLREHGGLDGFPKRKESIHDVFETGHSSTSLSAAAGMVIARDIKKEEFYVIPIIGDGALTGGMALEA
LNHIGDMGKDMIVILNDNDMSIAPNVGAIHNILGKLRTSDTFKQTKAKVDGTFFEELGFMYLGPINGHDIEEVITNLELA
KRTKGPVLLHIVTKKGKGYQPAELDSRGTWHGTGPYKVETGSFIKPAKRAASWSSVISNELIRLAEKDERIVAITPAMPV
GSKLEKFAKAFPERFFDVGIAEQHATTMAAGLATQGMKPFLTIYSTFLQRAYDQLVHDVCRQKLNVVIGIDRAGLVGADG
ETHQGIFDISFLNSIPNMTISMPKDEVEARQLMDTAFSYNDGPFAIRYPRGEAPGAQVAESNTLIPIGKWETIIQPIDAV
ILTFGPTIRLALKAAEQLELEGYHVGVINARYIKPLDEALLHQILKQKIPILTVEESLLKGGFGASVLEFIEASNYSDVV
MHRIGLPDEFISHGSVSIILESFGISTTGIVLKIKEMLAQSGKLRAKRL

Sequences:

>Translated_609_residues
MSCFYLDLLKIKDPSFMKQLDIQELEALAADIRAFLITSTSKSGGHIGPNLGVVELTIALHYSFNSPKDKFIWDVGHQSY
VHKILTGRASQFGTLREHGGLDGFPKRKESIHDVFETGHSSTSLSAAAGMVIARDIKKEEFYVIPIIGDGALTGGMALEA
LNHIGDMGKDMIVILNDNDMSIAPNVGAIHNILGKLRTSDTFKQTKAKVDGTFFEELGFMYLGPINGHDIEEVITNLELA
KRTKGPVLLHIVTKKGKGYQPAELDSRGTWHGTGPYKVETGSFIKPAKRAASWSSVISNELIRLAEKDERIVAITPAMPV
GSKLEKFAKAFPERFFDVGIAEQHATTMAAGLATQGMKPFLTIYSTFLQRAYDQLVHDVCRQKLNVVIGIDRAGLVGADG
ETHQGIFDISFLNSIPNMTISMPKDEVEARQLMDTAFSYNDGPFAIRYPRGEAPGAQVAESNTLIPIGKWETIIQPIDAV
ILTFGPTIRLALKAAEQLELEGYHVGVINARYIKPLDEALLHQILKQKIPILTVEESLLKGGFGASVLEFIEASNYSDVV
MHRIGLPDEFISHGSVSIILESFGISTTGIVLKIKEMLAQSGKLRAKRL
>Mature_608_residues
SCFYLDLLKIKDPSFMKQLDIQELEALAADIRAFLITSTSKSGGHIGPNLGVVELTIALHYSFNSPKDKFIWDVGHQSYV
HKILTGRASQFGTLREHGGLDGFPKRKESIHDVFETGHSSTSLSAAAGMVIARDIKKEEFYVIPIIGDGALTGGMALEAL
NHIGDMGKDMIVILNDNDMSIAPNVGAIHNILGKLRTSDTFKQTKAKVDGTFFEELGFMYLGPINGHDIEEVITNLELAK
RTKGPVLLHIVTKKGKGYQPAELDSRGTWHGTGPYKVETGSFIKPAKRAASWSSVISNELIRLAEKDERIVAITPAMPVG
SKLEKFAKAFPERFFDVGIAEQHATTMAAGLATQGMKPFLTIYSTFLQRAYDQLVHDVCRQKLNVVIGIDRAGLVGADGE
THQGIFDISFLNSIPNMTISMPKDEVEARQLMDTAFSYNDGPFAIRYPRGEAPGAQVAESNTLIPIGKWETIIQPIDAVI
LTFGPTIRLALKAAEQLELEGYHVGVINARYIKPLDEALLHQILKQKIPILTVEESLLKGGFGASVLEFIEASNYSDVVM
HRIGLPDEFISHGSVSIILESFGISTTGIVLKIKEMLAQSGKLRAKRL

Specific function: Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)

COG id: COG1154

COG function: function code HI; Deoxyxylulose-5-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family. DXPS subfamily

Homologues:

Organism=Homo sapiens, GI205277463, Length=652, Percent_Identity=24.079754601227, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI4507521, Length=652, Percent_Identity=24.079754601227, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI133778974, Length=636, Percent_Identity=24.8427672955975, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI225637463, Length=306, Percent_Identity=28.1045751633987, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI225637459, Length=306, Percent_Identity=28.1045751633987, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI225637461, Length=306, Percent_Identity=28.1045751633987, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786622, Length=621, Percent_Identity=45.7326892109501, Blast_Score=522, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17539652, Length=630, Percent_Identity=24.4444444444444, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24666278, Length=637, Percent_Identity=25.1177394034537, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45551847, Length=638, Percent_Identity=24.7648902821317, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45550715, Length=638, Percent_Identity=24.7648902821317, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645119, Length=595, Percent_Identity=24.7058823529412, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DXS_LISMC (C1L2S1)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758073.1
- ProteinModelPortal:   C1L2S1
- SMR:   C1L2S1
- GeneID:   7703261
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01374
- OMA:   VSNTGGH
- ProtClustDB:   PRK05444
- HAMAP:   MF_00315
- InterPro:   IPR005477
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR020826
- InterPro:   IPR005476
- InterPro:   IPR005474
- Gene3D:   G3DSA:3.40.50.920
- SMART:   SM00861
- TIGRFAMs:   TIGR00204

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; PF00456 Transketolase_N; SSF52922 Transketo_C_like

EC number: =2.2.1.7

Molecular weight: Translated: 66715; Mature: 66584

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00801 TRANSKETOLASE_1; PS00802 TRANSKETOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCFYLDLLKIKDPSFMKQLDIQELEALAADIRAFLITSTSKSGGHIGPNLGVVELTIAL
CCEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEE
HYSFNSPKDKFIWDVGHQSYVHKILTGRASQFGTLREHGGLDGFPKRKESIHDVFETGHS
EEECCCCCCCEEEECCHHHHHHHHHHCCCHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCC
STSLSAAAGMVIARDIKKEEFYVIPIIGDGALTGGMALEALNHIGDMGKDMIVILNDNDM
CCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
SIAPNVGAIHNILGKLRTSDTFKQTKAKVDGTFFEELGFMYLGPINGHDIEEVITNLELA
EECCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCEEECCCCCCCHHHHHHHHHHH
KRTKGPVLLHIVTKKGKGYQPAELDSRGTWHGTGPYKVETGSFIKPAKRAASWSSVISNE
HCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
LIRLAEKDERIVAITPAMPVGSKLEKFAKAFPERFFDVGIAEQHATTMAAGLATQGMKPF
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHH
LTIYSTFLQRAYDQLVHDVCRQKLNVVIGIDRAGLVGADGETHQGIFDISFLNSIPNMTI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCEEEEHHHHCCCCCEE
SMPKDEVEARQLMDTAFSYNDGPFAIRYPRGEAPGAQVAESNTLIPIGKWETIIQPIDAV
ECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
ILTFGPTIRLALKAAEQLELEGYHVGVINARYIKPLDEALLHQILKQKIPILTVEESLLK
HHHCCCHHHHHHHHHHHHCCCCEEEEEEECHHHCCHHHHHHHHHHHHCCCEEEECHHHHC
GGFGASVLEFIEASNYSDVVMHRIGLPDEFISHGSVSIILESFGISTTGIVLKIKEMLAQ
CCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHC
SGKLRAKRL
CCCCCCCCC
>Mature Secondary Structure 
SCFYLDLLKIKDPSFMKQLDIQELEALAADIRAFLITSTSKSGGHIGPNLGVVELTIAL
CEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEE
HYSFNSPKDKFIWDVGHQSYVHKILTGRASQFGTLREHGGLDGFPKRKESIHDVFETGHS
EEECCCCCCCEEEECCHHHHHHHHHHCCCHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCC
STSLSAAAGMVIARDIKKEEFYVIPIIGDGALTGGMALEALNHIGDMGKDMIVILNDNDM
CCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
SIAPNVGAIHNILGKLRTSDTFKQTKAKVDGTFFEELGFMYLGPINGHDIEEVITNLELA
EECCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCEEECCCCCCCHHHHHHHHHHH
KRTKGPVLLHIVTKKGKGYQPAELDSRGTWHGTGPYKVETGSFIKPAKRAASWSSVISNE
HCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
LIRLAEKDERIVAITPAMPVGSKLEKFAKAFPERFFDVGIAEQHATTMAAGLATQGMKPF
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHH
LTIYSTFLQRAYDQLVHDVCRQKLNVVIGIDRAGLVGADGETHQGIFDISFLNSIPNMTI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCEEEEHHHHCCCCCEE
SMPKDEVEARQLMDTAFSYNDGPFAIRYPRGEAPGAQVAESNTLIPIGKWETIIQPIDAV
ECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
ILTFGPTIRLALKAAEQLELEGYHVGVINARYIKPLDEALLHQILKQKIPILTVEESLLK
HHHCCCHHHHHHHHHHHHCCCCEEEEEEECHHHCCHHHHHHHHHHHHCCCEEEECHHHHC
GGFGASVLEFIEASNYSDVVMHRIGLPDEFISHGSVSIILESFGISTTGIVLKIKEMLAQ
CCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHC
SGKLRAKRL
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA