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The map label for this gene is cspL

Identifier: 226223965

GI number: 226223965

Start: 1370715

End: 1370921

Strand: Direct

Name: cspL

Synonym: Lm4b_01373

Alternate gene names: 226223965

Gene position: 1370715-1370921 (Clockwise)

Preceding gene: 226223964

Following gene: 226223966

Centisome position: 47.06

GC content: 43.0

Gene sequence:

>207_bases
ATGAACATGGAACAAGGTACAGTAAAATGGTTTAACGCAGAAAAAGGATTTGGTTTTATCGAACGCGAAAACGGTGACGA
TGTATTCGTACATTTCAGCGCTATCCAAGGCGACGGATTCAAATCTTTAGACGAAGGTCAAGCAGTAACTTTCGACGTTG
AAGAAGGCCAACGCGGACCTCAAGCAGCTAACGTTCAAAAAGCGTAA

Upstream 100 bases:

>100_bases
TATTTTTGTGGGTGAAAGATACGATTGTGAACAACTTTCCATCTCGTGCCGTTAAGCAAGAATAGTAAATAATTAGTGTG
CATAACACACGAGGAGGAAC

Downstream 100 bases:

>100_bases
TTCTATTTTTTGAATAAGAAAAAGCAAATCATTTCGGTGATTTGCTTTTTTATTTGCCTAAAATTATTTTACCTTGTTTG
GTTTAATGGTGATTGTTTGC

Product: cold shock protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 68; Mature: 68

Protein sequence:

>68_residues
MNMEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQAANVQKA

Sequences:

>Translated_68_residues
MNMEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQAANVQKA
>Mature_68_residues
MNMEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGPQAANVQKA

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=61, Percent_Identity=68.8524590163934, Blast_Score=88, Evalue=9e-20,
Organism=Escherichia coli, GI1787839, Length=65, Percent_Identity=63.0769230769231, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=61.5384615384615, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1786841, Length=66, Percent_Identity=62.1212121212121, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=62.1212121212121, Blast_Score=82, Evalue=4e-18,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=58.4615384615385, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=54.6875, Blast_Score=75, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7511; Mature: 7511

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGP
CCCCCCCEEEEECCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCC
QAANVQKA
CCCCCCCC
>Mature Secondary Structure
MNMEQGTVKWFNAEKGFGFIERENGDDVFVHFSAIQGDGFKSLDEGQAVTFDVEEGQRGP
CCCCCCCEEEEECCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCC
QAANVQKA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]