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The map label for this gene is gid

Identifier: 226223879

GI number: 226223879

Start: 1286285

End: 1287589

Strand: Direct

Name: gid

Synonym: Lm4b_01286

Alternate gene names: 226223879

Gene position: 1286285-1287589 (Clockwise)

Preceding gene: 226223878

Following gene: 226223880

Centisome position: 44.16

GC content: 40.0

Gene sequence:

>1305_bases
ATGGAAAAGAGTGTTAATGTAATTGGAGCAGGTTTAGCAGGAAGTGAAGCAGCATGGCAGTTAGTAAAACGTGGGGTAAA
GGTGGATTTATACGAAATGAGACCGGTAAAACAAACTCCGGCACATCATACAGATAAATTTGCAGAGCTAGTATGTACGA
ATTCCTTGCGAGCTAACGGTCTAACGAATGCTGTAGGAGTTATTAAAGAAGAAATGCGGATACTTGATTCTATTATCATT
GAAGCCGCTGACAAAGCATCTGTACCAGCTGGAGGGGCACTTGCGGTTGATCGACATGAATTTTCTGGTTATATTACAGA
CAAGGTGAAAAATCATCCACTCGTAACAGTGCATACGGAAGAAGTAACTACTATTCCAGAAGGTCCAACAATTATTGCGA
CCGGTCCACTTACAAGTCCTGCGCTTGCTGATGAAATTAAACAATTAACAGGGGAAGAGTATCTTTACTTTTATGATGCA
GCAGCACCAATAATTGAAAAAGATAGCATTGATATGGATAAAGTTTATTTGAAATCCCGCTATGATAAAGGAGAAGCTGC
TTACTTAAACTGCCCGATGTCAGAAGAAGAATTCAATGCTTTTTATGAAGCATTAGTAACGGCAGAAACGGCTGCTTTAA
AAGAATTTGAAAAAGAAGTGTTTTTCGAAGGATGTATGCCAATCGAAGTAATGGCAAAACGCGGAATTAAAACGATGTTA
TTTGGTCCATTAAAGCCCGTTGGTTTGGAAGATCCAAAAACTGGGAAAAGACCTTATGCTGTGTTGCAACTTAGACAAGA
TGACGCAGCGGGAACACTTTACAACATGGTAGGTTTCCAAACGCACCTTAAATGGGGTGAGCAAAAACGTGTTTTCGGAA
TGATTCCAGGACTTGAAAATGCTGAAATCGTACGTTATGGTGTAATGCACCGTAACACATTTATTAACTCACCAACTGTA
CTTGAACCAACTTACCAACTGAAAACACGCAATGATTTGTTCTTTGCTGGACAAATGACAGGTGTAGAGGGATATGTTGA
ATCCGCGGCTAGTGGACTTGCGGCGGGGATTAATGCGGCCAATTTTGTAGAGGAAAAAGAACTAGTGGTTTTCCCGGCAG
AAACGGCGATTGGTAGTCTAGCCCATTACATTACGAGTGCTAGTAAAAAATCGTTCCAACCAATGAATGTTAATTTTGGA
CTCTTCCCAGAGCTTGAAACAAAAATTCGTGCAAAACAAGAACGTAATGAAAAATTAGCTGAAAGAGCGCTAAATGCAAT
AAAAAGGGTTGCAGAACAACTATAA

Upstream 100 bases:

>100_bases
GTACAAATTGTGATTACAAAGAATCAACCCAACAATAATCGGAAAATGGCAACACTTGGATCGTTCTTGTGTTGCCATTG
TTGCGCGAGGAGGCGGACAA

Downstream 100 bases:

>100_bases
AAACACTATAATAATTGAGGACTATTCTTTGCTGTTCTTTTCATCACGATGGCTACTTTGGTATTTCAAGAGAGTTGATA
GTGGTGGAAAGACAGTTTTT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MEKSVNVIGAGLAGSEAAWQLVKRGVKVDLYEMRPVKQTPAHHTDKFAELVCTNSLRANGLTNAVGVIKEEMRILDSIII
EAADKASVPAGGALAVDRHEFSGYITDKVKNHPLVTVHTEEVTTIPEGPTIIATGPLTSPALADEIKQLTGEEYLYFYDA
AAPIIEKDSIDMDKVYLKSRYDKGEAAYLNCPMSEEEFNAFYEALVTAETAALKEFEKEVFFEGCMPIEVMAKRGIKTML
FGPLKPVGLEDPKTGKRPYAVLQLRQDDAAGTLYNMVGFQTHLKWGEQKRVFGMIPGLENAEIVRYGVMHRNTFINSPTV
LEPTYQLKTRNDLFFAGQMTGVEGYVESAASGLAAGINAANFVEEKELVVFPAETAIGSLAHYITSASKKSFQPMNVNFG
LFPELETKIRAKQERNEKLAERALNAIKRVAEQL

Sequences:

>Translated_434_residues
MEKSVNVIGAGLAGSEAAWQLVKRGVKVDLYEMRPVKQTPAHHTDKFAELVCTNSLRANGLTNAVGVIKEEMRILDSIII
EAADKASVPAGGALAVDRHEFSGYITDKVKNHPLVTVHTEEVTTIPEGPTIIATGPLTSPALADEIKQLTGEEYLYFYDA
AAPIIEKDSIDMDKVYLKSRYDKGEAAYLNCPMSEEEFNAFYEALVTAETAALKEFEKEVFFEGCMPIEVMAKRGIKTML
FGPLKPVGLEDPKTGKRPYAVLQLRQDDAAGTLYNMVGFQTHLKWGEQKRVFGMIPGLENAEIVRYGVMHRNTFINSPTV
LEPTYQLKTRNDLFFAGQMTGVEGYVESAASGLAAGINAANFVEEKELVVFPAETAIGSLAHYITSASKKSFQPMNVNFG
LFPELETKIRAKQERNEKLAERALNAIKRVAEQL
>Mature_434_residues
MEKSVNVIGAGLAGSEAAWQLVKRGVKVDLYEMRPVKQTPAHHTDKFAELVCTNSLRANGLTNAVGVIKEEMRILDSIII
EAADKASVPAGGALAVDRHEFSGYITDKVKNHPLVTVHTEEVTTIPEGPTIIATGPLTSPALADEIKQLTGEEYLYFYDA
AAPIIEKDSIDMDKVYLKSRYDKGEAAYLNCPMSEEEFNAFYEALVTAETAALKEFEKEVFFEGCMPIEVMAKRGIKTML
FGPLKPVGLEDPKTGKRPYAVLQLRQDDAAGTLYNMVGFQTHLKWGEQKRVFGMIPGLENAEIVRYGVMHRNTFINSPTV
LEPTYQLKTRNDLFFAGQMTGVEGYVESAASGLAAGINAANFVEEKELVVFPAETAIGSLAHYITSASKKSFQPMNVNFG
LFPELETKIRAKQERNEKLAERALNAIKRVAEQL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=43.3333333333333, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 47851; Mature: 47851

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSVNVIGAGLAGSEAAWQLVKRGVKVDLYEMRPVKQTPAHHTDKFAELVCTNSLRANG
CCCCCEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHCCC
LTNAVGVIKEEMRILDSIIIEAADKASVPAGGALAVDRHEFSGYITDKVKNHPLVTVHTE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHCCCEEEHHCCCCCEEEEECC
EVTTIPEGPTIIATGPLTSPALADEIKQLTGEEYLYFYDAAAPIIEKDSIDMDKVYLKSR
CEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHC
YDKGEAAYLNCPMSEEEFNAFYEALVTAETAALKEFEKEVFFEGCMPIEVMAKRGIKTML
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEE
FGPLKPVGLEDPKTGKRPYAVLQLRQDDAAGTLYNMVGFQTHLKWGEQKRVFGMIPGLEN
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
AEIVRYGVMHRNTFINSPTVLEPTYQLKTRNDLFFAGQMTGVEGYVESAASGLAAGINAA
CCEEEEEHHHCCCCCCCCCEECCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCHH
NFVEEKELVVFPAETAIGSLAHYITSASKKSFQPMNVNFGLFPELETKIRAKQERNEKLA
HHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ERALNAIKRVAEQL
HHHHHHHHHHHHHC
>Mature Secondary Structure
MEKSVNVIGAGLAGSEAAWQLVKRGVKVDLYEMRPVKQTPAHHTDKFAELVCTNSLRANG
CCCCCEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHCCC
LTNAVGVIKEEMRILDSIIIEAADKASVPAGGALAVDRHEFSGYITDKVKNHPLVTVHTE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHCCCEEEHHCCCCCEEEEECC
EVTTIPEGPTIIATGPLTSPALADEIKQLTGEEYLYFYDAAAPIIEKDSIDMDKVYLKSR
CEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHC
YDKGEAAYLNCPMSEEEFNAFYEALVTAETAALKEFEKEVFFEGCMPIEVMAKRGIKTML
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEE
FGPLKPVGLEDPKTGKRPYAVLQLRQDDAAGTLYNMVGFQTHLKWGEQKRVFGMIPGLEN
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
AEIVRYGVMHRNTFINSPTVLEPTYQLKTRNDLFFAGQMTGVEGYVESAASGLAAGINAA
CCEEEEEHHHCCCCCCCCCEECCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCHH
NFVEEKELVVFPAETAIGSLAHYITSASKKSFQPMNVNFGLFPELETKIRAKQERNEKLA
HHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ERALNAIKRVAEQL
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]