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The map label for this gene is topA

Identifier: 226223878

GI number: 226223878

Start: 1284144

End: 1286222

Strand: Direct

Name: topA

Synonym: Lm4b_01285

Alternate gene names: 226223878

Gene position: 1284144-1286222 (Clockwise)

Preceding gene: 226223877

Following gene: 226223879

Centisome position: 44.09

GC content: 37.57

Gene sequence:

>2079_bases
ATGGCAGATTATTTAGTGATTGTAGAGTCACCTGCAAAAGCAAAAACAATTGAAAAGTATTTAGGTAAGAAATTTAAAGT
GAAAGCCTCAATGGGACATGTTCGTGACTTACCTAAGAGTCAAATGGGTGTAGACACAGAGCATGACTACGAACCTCGTT
ATATTACGATTCGCGGAAAAGGCCCTGTTTTAAAAGAATTAAAACAAGCAGCCAAAAAAGCGAAAAAAGTCTATCTCGCA
GCCGATCCAGACCGCGAAGGAGAAGCAATTGCATGGCACTTAGCCAATAGCCTAGAGTTAGATCAATCAGACAAACTACG
TGTAGTATTTAACGAAATAACGAAGGAAGCAGTAAAAGAATCTTTCAAAACACCACGTAAAATTGACATGGATTTAGTCA
ATGCGCAACAAGCACGCAGAATTTTGGACCGTTTAGTTGGATATAATATCAGTCCTATTCTATGGAAAAAAGTGAAGAAA
GGTTTGAGTGCGGGGCGTGTTCAGTCTATCGCACTTCGAATCATTATTGATAGAGAAAAAGAAATCAATAATTTCAAACC
TGAAGAATACTGGACAATTGACGGTAACTTCCTTAAAGGGAAGAAAAAATTCCAAGCCAATTTTTATGGTGTAAATGGTA
AAAAGAAAAAGCTTTCCACTGCAGATGATGTAAAAGAAGTAATGTCTGCAATAAAAGGAAAATCATTTGACGTAACAGAT
GTAACGAAAAAAGAAAGACTTAGAAATCCAGCTGCACCATTCACTACTTCAAGTTTGCAACAAGAAGCAGCTCGAAAACT
AAATTATCGTACAAGAAAAACAATGATGCTTGCCCAACAATTGTATGAAGGAATTACACTTGGTAAACAAGGGACAGTCG
GCTTAATTACGTATATGCGTACAGACTCCACTCGTATTGCCGATTCCGCTATACTAGAAGCAAGCAATTACATTAAAGAA
ACCTATGGACCCGAATTTTCTCGTAATCATAAACGTTCAGATAAAAATGCTAAAGGTGCTCAAGATGCCCATGAAGCAAT
CAGACCTACAAGTGCAATGAGAAGTCCTCAAGAAGTAAAAGAATTTTTAACACGGGACCAACTTCGCTTATATCGTTTGA
TTTGGGAAAGATTTATCGCGAGCCAAATGACGCCAGCAGTTCTTGATACAATGCGCGTTGACTTAGACAACAATGGCGTG
AACTTCAGAGCAAATGGCTCTAAAATTAAATTCCACGGCTTCATGAAAGTCTATGTAGAAAGTAACGATGATAATACGGA
AGAAAAAGAAAATATCTTACCAGATTTGAAAAAAGGAGATAAGGTACAGTCCGAATCACTCGAACAACGGCAACATTTCA
CACAGCCACCTCCACGTTACACAGAAGCTAGATTAGTAAAAACGCTCGAAGAAATTGGTATTGGTCGACCATCAACTTAT
TCGCCGACACTCGACACAATTCAGCGCAGAAATTATGTTTCTTTAACGAATAAACGCTTTATTCCAACTGAATTAGGTGA
AATCGTTAATGAAATGATTGAAGAATATTTCCCAGAAATTTTAGATGTCAAATTCACTGCCAACATGGAAAGTGAGCTCG
ATGAAGTAGAGCACGGAGAAGTCGAATGGGTGAAAGTCATTGATGAATTTTATAAACGATTTGAACCAAATGTTATAAAA
GCAGATGCAGAAATGGAAAAAATCGAGATTAAAGATGAACCAGCCGGAATTGACTGTGATCTTTGTGGAGCTCCAATGGT
TTACAAAATGGGTAAATACGGTAAGTTCCTAGCTTGTAGCAGATTTCCTGATTGTCGTAATACGAAAGCAATCGTGAAAG
AAATCGGCGTTACGTGTCCGAAATGTGAGAAAGGACATGTAATAGAAAGAAAAAGTAAGAAAAAGCGTATTTTTTACGGT
TGTGATCGTTATCCAGATTGTGACTATGTATCATGGGATAAACCGGTCGAACGGGCTTGTCCGAAATGTGAGAAACGAGC
ATTAGTCGAGAAGAAATTGAAAAAAGGTGTACAAGTACAATGTACAAATTGTGATTACAAAGAATCAACCCAACAATAA

Upstream 100 bases:

>100_bases
GCATTGAAAACTTATTGAACGCGAGGCTAAGACTGTAATAGGATTAAGACAACGAGTAAAGCCCGTGTATTGACCCAAAT
CAGGGAGGAATATAATAGAT

Downstream 100 bases:

>100_bases
TCGGAAAATGGCAACACTTGGATCGTTCTTGTGTTGCCATTGTTGCGCGAGGAGGCGGACAAATGGAAAAGAGTGTTAAT
GTAATTGGAGCAGGTTTAGC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGKGPVLKELKQAAKKAKKVYLA
ADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKESFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKK
GLSAGRVQSIALRIIIDREKEINNFKPEEYWTIDGNFLKGKKKFQANFYGVNGKKKKLSTADDVKEVMSAIKGKSFDVTD
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMRTDSTRIADSAILEASNYIKE
TYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVKEFLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGV
NFRANGSKIKFHGFMKVYVESNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
SPTLDTIQRRNYVSLTNKRFIPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGEVEWVKVIDEFYKRFEPNVIK
ADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACSRFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYG
CDRYPDCDYVSWDKPVERACPKCEKRALVEKKLKKGVQVQCTNCDYKESTQQ

Sequences:

>Translated_692_residues
MADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGKGPVLKELKQAAKKAKKVYLA
ADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKESFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKK
GLSAGRVQSIALRIIIDREKEINNFKPEEYWTIDGNFLKGKKKFQANFYGVNGKKKKLSTADDVKEVMSAIKGKSFDVTD
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMRTDSTRIADSAILEASNYIKE
TYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVKEFLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGV
NFRANGSKIKFHGFMKVYVESNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
SPTLDTIQRRNYVSLTNKRFIPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGEVEWVKVIDEFYKRFEPNVIK
ADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACSRFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYG
CDRYPDCDYVSWDKPVERACPKCEKRALVEKKLKKGVQVQCTNCDYKESTQQ
>Mature_691_residues
ADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGKGPVLKELKQAAKKAKKVYLAA
DPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKESFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKKG
LSAGRVQSIALRIIIDREKEINNFKPEEYWTIDGNFLKGKKKFQANFYGVNGKKKKLSTADDVKEVMSAIKGKSFDVTDV
TKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMRTDSTRIADSAILEASNYIKET
YGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVKEFLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGVN
FRANGSKIKFHGFMKVYVESNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTYS
PTLDTIQRRNYVSLTNKRFIPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGEVEWVKVIDEFYKRFEPNVIKA
DAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACSRFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYGC
DRYPDCDYVSWDKPVERACPKCEKRALVEKKLKKGVQVQCTNCDYKESTQQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=627, Percent_Identity=25.6778309409888, Blast_Score=136, Evalue=9e-32,
Organism=Homo sapiens, GI4507635, Length=560, Percent_Identity=21.9642857142857, Blast_Score=97, Evalue=7e-20,
Organism=Escherichia coli, GI1787529, Length=752, Percent_Identity=37.6329787234042, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI48994931, Length=107, Percent_Identity=39.2523364485981, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788061, Length=520, Percent_Identity=23.4615384615385, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=559, Percent_Identity=25.9391771019678, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=506, Percent_Identity=23.9130434782609, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=481, Percent_Identity=23.4927234927235, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585251, Length=647, Percent_Identity=23.338485316847, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24640096, Length=513, Percent_Identity=22.8070175438596, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79432; Mature: 79301

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGK
CCCEEEEEECCCHHHHHHHHCCCEEEEEEHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPVLKELKQAAKKAKKVYLAADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKE
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
SFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKKGLSAGRVQSIALRIIIDREK
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHEEEEEEEECHH
EINNFKPEEYWTIDGNFLKGKKKFQANFYGVNGKKKKLSTADDVKEVMSAIKGKSFDVTD
HCCCCCCCCEEEECCCEECCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMR
CHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRIADSAILEASNYIKETYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHH
EFLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGVNFRANGSKIKFHGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCEEEEECCCEEEEEEEEEEEEE
SNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SPTLDTIQRRNYVSLTNKRFIPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGE
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHCCCC
VEWVKVIDEFYKRFEPNVIKADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACS
HHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEC
RFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYGCDRYPDCDYVSWDKPVERAC
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHC
PKCEKRALVEKKLKKGVQVQCTNCDYKESTQQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHCCCC
>Mature Secondary Structure 
ADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGK
CCEEEEEECCCHHHHHHHHCCCEEEEEEHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPVLKELKQAAKKAKKVYLAADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKE
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
SFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKKGLSAGRVQSIALRIIIDREK
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHEEEEEEEECHH
EINNFKPEEYWTIDGNFLKGKKKFQANFYGVNGKKKKLSTADDVKEVMSAIKGKSFDVTD
HCCCCCCCCEEEECCCEECCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMR
CHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRIADSAILEASNYIKETYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHH
EFLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGVNFRANGSKIKFHGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCEEEEECCCEEEEEEEEEEEEE
SNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SPTLDTIQRRNYVSLTNKRFIPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGE
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHCCCC
VEWVKVIDEFYKRFEPNVIKADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACS
HHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEC
RFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYGCDRYPDCDYVSWDKPVERAC
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHC
PKCEKRALVEKKLKKGVQVQCTNCDYKESTQQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]