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The map label for this gene is rnhB

Identifier: 226223876

GI number: 226223876

Start: 1282092

End: 1282877

Strand: Direct

Name: rnhB

Synonym: Lm4b_01283

Alternate gene names: 226223876

Gene position: 1282092-1282877 (Clockwise)

Preceding gene: 226223875

Following gene: 226223877

Centisome position: 44.02

GC content: 37.66

Gene sequence:

>786_bases
ATGAGCGATTCCATATCCGTTATCCGAGAAAAACTAAACCAAGTCACATCAGAACATGATCCCTTTTTTCGGAAATGCAT
GCAAGATGAGCGTAAAGGTGTAGAAAAACTTCTGGAAACTACAAGGAGAAAATGGGAAAAAGAAGCGCAATTACGGAACA
AGCTTGAAGAAATGAAACAATATGAAACAGATTTATTCCAACAAGGATACAAGTATATCGCTGGAGTGGATGAAGTGGGA
CGTGGGCCTCTCGCAGGACCAGTTGTAGCAGCAGCCGTCATTTTACCAGCTGATTTTTCTGTTGTCGGGATAAATGATTC
TAAACAATTGAGTGAAGCAAAACGTGATGCTTTGTTTGAAACGATAAAAAAAGAAGCAATTGCTATCGGAGTAGGGATTA
TAGAACATGATGTAATTGATCAAGTAAATATTTATGAGGCAACGAAACTTGCTATGCGTGAAGCGTTAGATCAATTAACA
CCAGAACCTGATTTTGTTCTAATAGATGCAATGCCACTAAGATATACCGAGGCAGAACTATCTCTTATCAAAGGGGATAC
AAAAAGCATTTCGATTGCTGCAGCGTCTATCATCGCAAAGGTAACAAGAGATAGATTAATGCAGATGTACGATGAAAAGT
ATCCCGGGTATGATTTTGCAAACAATATGGGCTACGGCACAAAAAAACATTTACTTGGTTTAGATACAATTGGTATATGT
CCTATCCATCGTATAAGTTTTGCGCCTGTAAAAGAAGCAAAGTTGCATTTTGATAGTTTAAAATAA

Upstream 100 bases:

>100_bases
CAGATTATTCTCGCGCAGCCGAAACGGTAGTTCGAGAAATTCGTCAGCAAAAGTTAGGGAGAATGTCGTTTGATTTCCCA
AATTGGGAAGATGAAGTCGA

Downstream 100 bases:

>100_bases
CTAAATCATATAGAGAAAAACGTATTTTCAACATGGAAAATACGTTTTTTTATGCCGTTTTATAGGTTTTGGTCATTTTA
CAAAAAAGCCCAATGTTTGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSDSISVIREKLNQVTSEHDPFFRKCMQDERKGVEKLLETTRRKWEKEAQLRNKLEEMKQYETDLFQQGYKYIAGVDEVG
RGPLAGPVVAAAVILPADFSVVGINDSKQLSEAKRDALFETIKKEAIAIGVGIIEHDVIDQVNIYEATKLAMREALDQLT
PEPDFVLIDAMPLRYTEAELSLIKGDTKSISIAAASIIAKVTRDRLMQMYDEKYPGYDFANNMGYGTKKHLLGLDTIGIC
PIHRISFAPVKEAKLHFDSLK

Sequences:

>Translated_261_residues
MSDSISVIREKLNQVTSEHDPFFRKCMQDERKGVEKLLETTRRKWEKEAQLRNKLEEMKQYETDLFQQGYKYIAGVDEVG
RGPLAGPVVAAAVILPADFSVVGINDSKQLSEAKRDALFETIKKEAIAIGVGIIEHDVIDQVNIYEATKLAMREALDQLT
PEPDFVLIDAMPLRYTEAELSLIKGDTKSISIAAASIIAKVTRDRLMQMYDEKYPGYDFANNMGYGTKKHLLGLDTIGIC
PIHRISFAPVKEAKLHFDSLK
>Mature_260_residues
SDSISVIREKLNQVTSEHDPFFRKCMQDERKGVEKLLETTRRKWEKEAQLRNKLEEMKQYETDLFQQGYKYIAGVDEVGR
GPLAGPVVAAAVILPADFSVVGINDSKQLSEAKRDALFETIKKEAIAIGVGIIEHDVIDQVNIYEATKLAMREALDQLTP
EPDFVLIDAMPLRYTEAELSLIKGDTKSISIAAASIIAKVTRDRLMQMYDEKYPGYDFANNMGYGTKKHLLGLDTIGICP
IHRISFAPVKEAKLHFDSLK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=188, Percent_Identity=46.8085106382979, Blast_Score=150, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_LISMC (C1L2I1)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002757983.1
- ProteinModelPortal:   C1L2I1
- SMR:   C1L2I1
- GeneID:   7701885
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01283
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 29396; Mature: 29265

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSISVIREKLNQVTSEHDPFFRKCMQDERKGVEKLLETTRRKWEKEAQLRNKLEEMKQ
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETDLFQQGYKYIAGVDEVGRGPLAGPVVAAAVILPADFSVVGINDSKQLSEAKRDALFE
HHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
TIKKEAIAIGVGIIEHDVIDQVNIYEATKLAMREALDQLTPEPDFVLIDAMPLRYTEAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHH
SLIKGDTKSISIAAASIIAKVTRDRLMQMYDEKYPGYDFANNMGYGTKKHLLGLDTIGIC
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCC
PIHRISFAPVKEAKLHFDSLK
CHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SDSISVIREKLNQVTSEHDPFFRKCMQDERKGVEKLLETTRRKWEKEAQLRNKLEEMKQ
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETDLFQQGYKYIAGVDEVGRGPLAGPVVAAAVILPADFSVVGINDSKQLSEAKRDALFE
HHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
TIKKEAIAIGVGIIEHDVIDQVNIYEATKLAMREALDQLTPEPDFVLIDAMPLRYTEAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHH
SLIKGDTKSISIAAASIIAKVTRDRLMQMYDEKYPGYDFANNMGYGTKKHLLGLDTIGIC
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCC
PIHRISFAPVKEAKLHFDSLK
CHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA