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The map label for this gene is gatD [H]

Identifier: 226223131

GI number: 226223131

Start: 537292

End: 538338

Strand: Direct

Name: gatD [H]

Synonym: Lm4b_00528

Alternate gene names: 226223131

Gene position: 537292-538338 (Clockwise)

Preceding gene: 226223130

Following gene: 226223132

Centisome position: 18.45

GC content: 40.78

Gene sequence:

>1047_bases
ATGAAAGCTTTAAAATTATATGGAAAAAGAGATTTGCGTTATGAAGAAGCGGATATGCCAACAATCGAACAGCCAGATGA
TGTGATTTTGAAAGTGAAAACGGTTGGGATTTGCGGATCTGATATTTCGAGATACAGTAAACTTGGTCCGTATGTACCAG
GAATGGTGTGGGGACATGAATTTTCAGGAGAAGTGATTGAGGTTGGTAGTGAGGTTACAGATATTGAGATTGGTGATAGG
GCTGCCGGATGCCCAGCACTCTATTGCGGAGAGTGTGAATACTGTAAAAAAGGCGAATTCGCCCGCTGTCGGAAGCTAAC
AGTAATCGGAGCAAGACATCCCGGAGCCTATGCTGAGTATATCAAACTTCCAGCAGAAAATGTAGTAAAAATACCAGACG
AATTAGATTATGAAGCTGCTGCACTTGTCGAGCCTTCCGCAGTGGTCGTTCATGGGTTTTATCATACGAAATTACAAGCA
GGCGATGATGTCGTCGTTGTCGGAAGCGGCAATATAGGTTTGCTAGCAATTCAATGGGCAAAAGTTTTTGGCGCAAAAAG
AGTTATTGCAATTGATGTAGACGATAAAAAGTTAGCTCTTGCAAAAGAAGTAGGAGCAGACGTCGTTATTAATTCTTTAA
AAGAAGATCCATTAGAAGTAGTCGCAGCACATACAGATGGTCTAAACGCTGATTTAGTTGTAGAAGCTGCGGGATCACCT
ATTACATCTGCCCAAGTTTTTGCCTATGCGAAAAAAGGTGGAGGCGTCGTTTTCCTAGGGATTCCATACGCCGATGTAAC
AATCGAACGTTTCTATTTTGAAAAAATTGTTCGGAGTGAGCTTACTGTTTGGGGCTCATGGAATGCAATTTCTGCACCAT
TTCCAGGAAAAGAATGGCAAACAACCATTCATTTCCTAGCCAATAAACAAATCAAAATTGAACCAATGATTACACATCGT
CTTTCACTTGCTGAAGGACCAGAAGTTTTTGAGCGAATCTATGAAAGAAATGAATTTTTCGGGAAAGTATTATTTTTCCC
AGAATAA

Upstream 100 bases:

>100_bases
GCACACTTGCAGGCGCGGATTTAATCGTTGTAGGATCTTACTTATTTGAAGGAGATATCGAAGCGAATTTGGAGAAGTTA
TCGAAAGGAGTTTTAACAGA

Downstream 100 bases:

>100_bases
TAGGGGGATTTTAGGATGAAAAAGAAGAAAATTTATGTTTGTTGTGGGACTGGAATTGCAACTTCCACTGTTATTTCCAA
AAAAGTGCGTGCGGTACTAG

Product: polyol (sorbitol) dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKALKLYGKRDLRYEEADMPTIEQPDDVILKVKTVGICGSDISRYSKLGPYVPGMVWGHEFSGEVIEVGSEVTDIEIGDR
AAGCPALYCGECEYCKKGEFARCRKLTVIGARHPGAYAEYIKLPAENVVKIPDELDYEAAALVEPSAVVVHGFYHTKLQA
GDDVVVVGSGNIGLLAIQWAKVFGAKRVIAIDVDDKKLALAKEVGADVVINSLKEDPLEVVAAHTDGLNADLVVEAAGSP
ITSAQVFAYAKKGGGVVFLGIPYADVTIERFYFEKIVRSELTVWGSWNAISAPFPGKEWQTTIHFLANKQIKIEPMITHR
LSLAEGPEVFERIYERNEFFGKVLFFPE

Sequences:

>Translated_348_residues
MKALKLYGKRDLRYEEADMPTIEQPDDVILKVKTVGICGSDISRYSKLGPYVPGMVWGHEFSGEVIEVGSEVTDIEIGDR
AAGCPALYCGECEYCKKGEFARCRKLTVIGARHPGAYAEYIKLPAENVVKIPDELDYEAAALVEPSAVVVHGFYHTKLQA
GDDVVVVGSGNIGLLAIQWAKVFGAKRVIAIDVDDKKLALAKEVGADVVINSLKEDPLEVVAAHTDGLNADLVVEAAGSP
ITSAQVFAYAKKGGGVVFLGIPYADVTIERFYFEKIVRSELTVWGSWNAISAPFPGKEWQTTIHFLANKQIKIEPMITHR
LSLAEGPEVFERIYERNEFFGKVLFFPE
>Mature_348_residues
MKALKLYGKRDLRYEEADMPTIEQPDDVILKVKTVGICGSDISRYSKLGPYVPGMVWGHEFSGEVIEVGSEVTDIEIGDR
AAGCPALYCGECEYCKKGEFARCRKLTVIGARHPGAYAEYIKLPAENVVKIPDELDYEAAALVEPSAVVVHGFYHTKLQA
GDDVVVVGSGNIGLLAIQWAKVFGAKRVIAIDVDDKKLALAKEVGADVVINSLKEDPLEVVAAHTDGLNADLVVEAAGSP
ITSAQVFAYAKKGGGVVFLGIPYADVTIERFYFEKIVRSELTVWGSWNAISAPFPGKEWQTTIHFLANKQIKIEPMITHR
LSLAEGPEVFERIYERNEFFGKVLFFPE

Specific function: Converts galactitol 1-phosphate to tagatose 6-phosphate [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=338, Percent_Identity=31.9526627218935, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4501929, Length=373, Percent_Identity=27.0777479892761, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI34577061, Length=346, Percent_Identity=28.0346820809249, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI71565152, Length=345, Percent_Identity=22.8985507246377, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI156523966, Length=347, Percent_Identity=25.3602305475504, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4501939, Length=349, Percent_Identity=25.214899713467, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI71565154, Length=340, Percent_Identity=25.5882352941176, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4501933, Length=364, Percent_Identity=27.4725274725275, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788407, Length=336, Percent_Identity=37.5, Blast_Score=227, Evalue=9e-61,
Organism=Escherichia coli, GI1788073, Length=328, Percent_Identity=32.0121951219512, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1790045, Length=356, Percent_Identity=32.5842696629214, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788075, Length=356, Percent_Identity=29.2134831460674, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI226510992, Length=348, Percent_Identity=29.5977011494253, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=29.6829971181556, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1786825, Length=396, Percent_Identity=28.030303030303, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI87082125, Length=333, Percent_Identity=27.3273273273273, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081918, Length=315, Percent_Identity=33.968253968254, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790718, Length=307, Percent_Identity=27.0358306188925, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786552, Length=339, Percent_Identity=28.6135693215339, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082401, Length=320, Percent_Identity=25.9375, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786518, Length=250, Percent_Identity=28.8, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=337, Percent_Identity=34.4213649851632, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17562878, Length=221, Percent_Identity=39.8190045248869, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71988145, Length=248, Percent_Identity=31.4516129032258, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17562584, Length=273, Percent_Identity=29.6703296703297, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=270, Percent_Identity=29.6296296296296, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25146526, Length=356, Percent_Identity=26.123595505618, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71997431, Length=325, Percent_Identity=26.4615384615385, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565904, Length=324, Percent_Identity=26.2345679012346, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=354, Percent_Identity=31.638418079096, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319955, Length=354, Percent_Identity=31.638418079096, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323099, Length=328, Percent_Identity=32.0121951219512, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319258, Length=237, Percent_Identity=29.1139240506329, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6319257, Length=387, Percent_Identity=25.0645994832041, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319621, Length=340, Percent_Identity=27.9411764705882, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=346, Percent_Identity=28.3236994219653, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323961, Length=352, Percent_Identity=28.125, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=339, Percent_Identity=28.023598820059, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=362, Percent_Identity=26.5193370165746, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319520, Length=278, Percent_Identity=28.7769784172662, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319949, Length=326, Percent_Identity=27.3006134969325, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137530, Length=335, Percent_Identity=28.955223880597, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17737897, Length=339, Percent_Identity=28.9085545722714, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI221457811, Length=162, Percent_Identity=30.2469135802469, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550770, Length=162, Percent_Identity=30.2469135802469, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551930, Length=162, Percent_Identity=30.2469135802469, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17737895, Length=344, Percent_Identity=24.7093023255814, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.251 [H]

Molecular weight: Translated: 38202; Mature: 38202

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALKLYGKRDLRYEEADMPTIEQPDDVILKVKTVGICGSDISRYSKLGPYVPGMVWGHE
CCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHCCCCCCCEEECCC
FSGEVIEVGSEVTDIEIGDRAAGCPALYCGECEYCKKGEFARCRKLTVIGARHPGAYAEY
CCCCCEECCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCHHEEEEEEEECCCCCCHHHH
IKLPAENVVKIPDELDYEAAALVEPSAVVVHGFYHTKLQAGDDVVVVGSGNIGLLAIQWA
EECCCHHEEECCCCCCCCEEEEECCCEEEEEEEEEEEECCCCCEEEEECCCEEEEEEEEH
KVFGAKRVIAIDVDDKKLALAKEVGADVVINSLKEDPLEVVAAHTDGLNADLVVEAAGSP
HHHCCCEEEEEECCCCHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCC
ITSAQVFAYAKKGGGVVFLGIPYADVTIERFYFEKIVRSELTVWGSWNAISAPFPGKEWQ
CCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCCHHH
TTIHFLANKQIKIEPMITHRLSLAEGPEVFERIYERNEFFGKVLFFPE
HHEEEEECCEEEEEEEHEEEECCCCCHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MKALKLYGKRDLRYEEADMPTIEQPDDVILKVKTVGICGSDISRYSKLGPYVPGMVWGHE
CCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHCCCCCCCEEECCC
FSGEVIEVGSEVTDIEIGDRAAGCPALYCGECEYCKKGEFARCRKLTVIGARHPGAYAEY
CCCCCEECCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCHHEEEEEEEECCCCCCHHHH
IKLPAENVVKIPDELDYEAAALVEPSAVVVHGFYHTKLQAGDDVVVVGSGNIGLLAIQWA
EECCCHHEEECCCCCCCCEEEEECCCEEEEEEEEEEEECCCCCEEEEECCCEEEEEEEEH
KVFGAKRVIAIDVDDKKLALAKEVGADVVINSLKEDPLEVVAAHTDGLNADLVVEAAGSP
HHHCCCEEEEEECCCCHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCC
ITSAQVFAYAKKGGGVVFLGIPYADVTIERFYFEKIVRSELTVWGSWNAISAPFPGKEWQ
CCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCCHHH
TTIHFLANKQIKIEPMITHRLSLAEGPEVFERIYERNEFFGKVLFFPE
HHEEEEECCEEEEEEEHEEEECCCCCHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]