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The map label for this gene is cysK

Identifier: 226222852

GI number: 226222852

Start: 227441

End: 228367

Strand: Direct

Name: cysK

Synonym: Lm4b_00221

Alternate gene names: 226222852

Gene position: 227441-228367 (Clockwise)

Preceding gene: 226222851

Following gene: 226222853

Centisome position: 7.81

GC content: 41.75

Gene sequence:

>927_bases
ATGACAATTGCAAATTCAATCACTGATTTAATTGGAAAGACACCTATTGTGAAACTTAATCGTTTACCAGAAGCAGGAAG
CGCGGATGTATACGTAAAATTAGAATTCCAAAATCCAGGTGGCAGCGTAAAAGACCGTATTGCTAACGCGATGATCGAAA
GCGCTGAAAAATCTGGTGCATTAAAACCAGGCGATACAATCATTGAGCCAACTAGTGGGAATACTGGTATCGGCCTAGCA
ATGGTAGCAGCAGCAAAAGGCTACCAAGCGATTTTCGTAATGCCAGAAACAATGAGCTTAGAACGTCGCAAATTACTTCA
AGCTTACGGTGCAAAATTAGTTTTAACACCAGGTCCAGACGGCATGAAAGGCGCAATTGCTAAAGCGGAAGAGCTTGCAA
AAGAAAATAACTATTTTGTTCCACAACAATTCCACAACCCAGCAAATCCAGCTGTCCATGAAGAAACAACTGGACCAGAA
ATCGTTGAAGCTTTCGGTAAAGATGGCTTAGATGCTTTCATCGCGGGAGTTGGAACAGGTGGAACAGTAACTGGTGTAGG
ACACGTACTTAAAAAGAACTACCCAGATGTTAAAATCTATGCTCTTGAACCAGAAGAATCCCCAGTACTTAGCGGTGGTT
CTCCATCACCTCATAAAATCCAAGGTATCGGTGCAGGCTTCGTTCCGGATACATTAGATACAAAAGTGTATGACGGCATT
CTAAAAGTTTCAAGTGAAGATGCTCTAGAAACAGCACGTGAAGTAGCGAAAAAAGAAGGTATCTTAGTAGGTATTTCTTC
CGGAGCAACTGTTAAAGCAGCTCTAGACCTTGCAAAAGAACTTGGTGCTGGCAAAAAAGTACTAGCAATCGTTGCAAGTA
ACGGCGAACGCTACCTAAGCACACCTCTTTATAATTTTGAAGATTAA

Upstream 100 bases:

>100_bases
TAATTGGTTGCTCCTTTTTAAAAGAGGTTAAACTTGACAAAACAGGTCGGAATTAATTAAACTAGATGAAAGACCTAAAA
TAGATAGGAGTGCTTTAATA

Downstream 100 bases:

>100_bases
TAATCAGAGAGCCTAGATTTTCTAGGTTCTTTTTTTATGAAAAGAAATAAATGCATTTCTTACTATTTTCTAATCAAACG
CACAGCGGAAACTTTTTTTT

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTIANSITDLIGKTPIVKLNRLPEAGSADVYVKLEFQNPGGSVKDRIANAMIESAEKSGALKPGDTIIEPTSGNTGIGLA
MVAAAKGYQAIFVMPETMSLERRKLLQAYGAKLVLTPGPDGMKGAIAKAEELAKENNYFVPQQFHNPANPAVHEETTGPE
IVEAFGKDGLDAFIAGVGTGGTVTGVGHVLKKNYPDVKIYALEPEESPVLSGGSPSPHKIQGIGAGFVPDTLDTKVYDGI
LKVSSEDALETAREVAKKEGILVGISSGATVKAALDLAKELGAGKKVLAIVASNGERYLSTPLYNFED

Sequences:

>Translated_308_residues
MTIANSITDLIGKTPIVKLNRLPEAGSADVYVKLEFQNPGGSVKDRIANAMIESAEKSGALKPGDTIIEPTSGNTGIGLA
MVAAAKGYQAIFVMPETMSLERRKLLQAYGAKLVLTPGPDGMKGAIAKAEELAKENNYFVPQQFHNPANPAVHEETTGPE
IVEAFGKDGLDAFIAGVGTGGTVTGVGHVLKKNYPDVKIYALEPEESPVLSGGSPSPHKIQGIGAGFVPDTLDTKVYDGI
LKVSSEDALETAREVAKKEGILVGISSGATVKAALDLAKELGAGKKVLAIVASNGERYLSTPLYNFED
>Mature_307_residues
TIANSITDLIGKTPIVKLNRLPEAGSADVYVKLEFQNPGGSVKDRIANAMIESAEKSGALKPGDTIIEPTSGNTGIGLAM
VAAAKGYQAIFVMPETMSLERRKLLQAYGAKLVLTPGPDGMKGAIAKAEELAKENNYFVPQQFHNPANPAVHEETTGPEI
VEAFGKDGLDAFIAGVGTGGTVTGVGHVLKKNYPDVKIYALEPEESPVLSGGSPSPHKIQGIGAGFVPDTLDTKVYDGIL
KVSSEDALETAREVAKKEGILVGISSGATVKAALDLAKELGAGKKVLAIVASNGERYLSTPLYNFED

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=302, Percent_Identity=42.0529801324503, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI295821200, Length=302, Percent_Identity=42.0529801324503, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4557415, Length=302, Percent_Identity=42.0529801324503, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1788754, Length=312, Percent_Identity=54.8076923076923, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=43.421052631579, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=50.6666666666667, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI115535073, Length=307, Percent_Identity=53.0944625407166, Blast_Score=273, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=49.1694352159468, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=49.4915254237288, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17561720, Length=301, Percent_Identity=45.514950166113, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI32566672, Length=215, Percent_Identity=49.7674418604651, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17534315, Length=292, Percent_Identity=41.7808219178082, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=291, Percent_Identity=40.893470790378, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=46.1538461538462, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996324, Length=341, Percent_Identity=26.099706744868, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=38.2445141065831, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=325, Percent_Identity=35.3846153846154, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=39.4822006472492, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=39.4822006472492, Blast_Score=201, Evalue=7e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32204; Mature: 32073

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIANSITDLIGKTPIVKLNRLPEAGSADVYVKLEFQNPGGSVKDRIANAMIESAEKSGA
CCCHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
LKPGDTIIEPTSGNTGIGLAMVAAAKGYQAIFVMPETMSLERRKLLQAYGAKLVLTPGPD
CCCCCCEEECCCCCCCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCC
GMKGAIAKAEELAKENNYFVPQQFHNPANPAVHEETTGPEIVEAFGKDGLDAFIAGVGTG
CHHHHHHHHHHHHHHCCEECCHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHEECCCC
GTVTGVGHVLKKNYPDVKIYALEPEESPVLSGGSPSPHKIQGIGAGFVPDTLDTKVYDGI
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
LKVSSEDALETAREVAKKEGILVGISSGATVKAALDLAKELGAGKKVLAIVASNGERYLS
HCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
TPLYNFED
CCCCCCCC
>Mature Secondary Structure 
TIANSITDLIGKTPIVKLNRLPEAGSADVYVKLEFQNPGGSVKDRIANAMIESAEKSGA
CCHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
LKPGDTIIEPTSGNTGIGLAMVAAAKGYQAIFVMPETMSLERRKLLQAYGAKLVLTPGPD
CCCCCCEEECCCCCCCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCC
GMKGAIAKAEELAKENNYFVPQQFHNPANPAVHEETTGPEIVEAFGKDGLDAFIAGVGTG
CHHHHHHHHHHHHHHCCEECCHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHEECCCC
GTVTGVGHVLKKNYPDVKIYALEPEESPVLSGGSPSPHKIQGIGAGFVPDTLDTKVYDGI
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
LKVSSEDALETAREVAKKEGILVGISSGATVKAALDLAKELGAGKKVLAIVASNGERYLS
HCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
TPLYNFED
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA