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The map label for this gene is prs

Identifier: 226222828

GI number: 226222828

Start: 199326

End: 200282

Strand: Direct

Name: prs

Synonym: Lm4b_00196

Alternate gene names: 226222828

Gene position: 199326-200282 (Clockwise)

Preceding gene: 226222827

Following gene: 226222831

Centisome position: 6.84

GC content: 39.08

Gene sequence:

>957_bases
ATGTCAAACGAGTATTTTGATCCAAAGTTGAAGATTTTCTCGCTAAATTCTAATCGTGAACTAGCTGAAGAGATTGCGAA
AGAAGTTGGTATTGAGTTAGGGAAATCAAGCGTTACTCATTTTAGTGATGGAGAAATCCAAATTAACATTGAAGAAAGTA
TCCGTGGTTGTCATGTATATGTTATTCAATCAACGAGTAATCCTGTAAACCAGAATTTAATGGAACTTTTGATCATGATT
GATGCGTTGAAACGCGCTTCCGCAGCAACAATTAATATTGTTATGCCTTACTATGGTTACGCACGTCAAGACCGTAAAGC
AAGAAGTCGTGAACCAATCACAGCGAAATTAGTAGCAAACTTAATCGAAACTGCTGGTGCAACTAGAATGATTACACTTG
ATATGCATGCACCGCAAATCCAAGGTTTCTTTGATATTCCAATCGATCACTTGAATGCAGTTCGCCTTCTAAGTGACTAT
TTCAGTGAACGTCATTTAGGTGATGATCTAGTAGTAGTTTCCCCTGACCATGGTGGAGTTACTCGAGCTCGTAAAATGGC
TGACCGTTTGAAAGCGCCGATTGCTATTATTGATAAGCGTCGTCCGCGTCCAAACGTAGCTGAAGTAATGAACATCGTTG
GTAATGTAGAAGGGAAAGTTTGTATCATTATTGATGATATTATTGATACAGCTGGAACAATCACACTTGCTGCTAAAGCA
TTGCGTGAAGCAGGCGCAACAAAAGTTTACGCATGTTGTTCGCACCCAGTTCTTTCAGGTCCTGCTATGAAACGTATTGA
AGAATCGCCAATCGAAAAATTAGTTGTTACAAACTCCATCGCTCTTCCAGAAGAAAAATGGATTGACAAAATGGAACAAT
TATCTGTTGCAGCTCTTCTTGGCGAAGCAATCGTTCGCGTTCATGAAAATGCTTCTGTAAGTTCTTTATTCGAATAA

Upstream 100 bases:

>100_bases
AACAAGACAATAAACTTGGTTATGCGAAACATTTGAATCACGGTAAATAATTTCCATTTGGGAATTTTAAAATTTTAATA
AAAAATAGTGGAGGCTGATT

Downstream 100 bases:

>100_bases
AATATAAAACAGGATGCCTCATTGAGACATCCTGTTTTTTGATTTAATTTAATTTTCCCCAAGTAGCAGGACATGCTAAA
TAAAACCATTCATCTAATTT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 1; Phosphoribosyl pyrophosphate synthase 1; P-Rib-PP synthase 1; PRPP synthase 1 [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSNEYFDPKLKIFSLNSNRELAEEIAKEVGIELGKSSVTHFSDGEIQINIEESIRGCHVYVIQSTSNPVNQNLMELLIMI
DALKRASAATINIVMPYYGYARQDRKARSREPITAKLVANLIETAGATRMITLDMHAPQIQGFFDIPIDHLNAVRLLSDY
FSERHLGDDLVVVSPDHGGVTRARKMADRLKAPIAIIDKRRPRPNVAEVMNIVGNVEGKVCIIIDDIIDTAGTITLAAKA
LREAGATKVYACCSHPVLSGPAMKRIEESPIEKLVVTNSIALPEEKWIDKMEQLSVAALLGEAIVRVHENASVSSLFE

Sequences:

>Translated_318_residues
MSNEYFDPKLKIFSLNSNRELAEEIAKEVGIELGKSSVTHFSDGEIQINIEESIRGCHVYVIQSTSNPVNQNLMELLIMI
DALKRASAATINIVMPYYGYARQDRKARSREPITAKLVANLIETAGATRMITLDMHAPQIQGFFDIPIDHLNAVRLLSDY
FSERHLGDDLVVVSPDHGGVTRARKMADRLKAPIAIIDKRRPRPNVAEVMNIVGNVEGKVCIIIDDIIDTAGTITLAAKA
LREAGATKVYACCSHPVLSGPAMKRIEESPIEKLVVTNSIALPEEKWIDKMEQLSVAALLGEAIVRVHENASVSSLFE
>Mature_317_residues
SNEYFDPKLKIFSLNSNRELAEEIAKEVGIELGKSSVTHFSDGEIQINIEESIRGCHVYVIQSTSNPVNQNLMELLIMID
ALKRASAATINIVMPYYGYARQDRKARSREPITAKLVANLIETAGATRMITLDMHAPQIQGFFDIPIDHLNAVRLLSDYF
SERHLGDDLVVVSPDHGGVTRARKMADRLKAPIAIIDKRRPRPNVAEVMNIVGNVEGKVCIIIDDIIDTAGTITLAAKAL
REAGATKVYACCSHPVLSGPAMKRIEESPIEKLVVTNSIALPEEKWIDKMEQLSVAALLGEAIVRVHENASVSSLFE

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=317, Percent_Identity=46.6876971608833, Blast_Score=278, Evalue=4e-75,
Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=46.1783439490446, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=45.8333333333333, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=45.7413249211356, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI4506133, Length=345, Percent_Identity=37.6811594202899, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=36.6279069767442, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI310128524, Length=137, Percent_Identity=35.036496350365, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI310115209, Length=137, Percent_Identity=35.036496350365, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI310118259, Length=137, Percent_Identity=35.036496350365, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI310119946, Length=137, Percent_Identity=35.036496350365, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1787458, Length=314, Percent_Identity=47.4522292993631, Blast_Score=304, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=48.0769230769231, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=48.0769230769231, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=48.0769230769231, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=48.0519480519481, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17570245, Length=340, Percent_Identity=34.1176470588235, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321776, Length=311, Percent_Identity=42.7652733118971, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319403, Length=311, Percent_Identity=42.7652733118971, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6320946, Length=310, Percent_Identity=41.9354838709677, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6324511, Length=272, Percent_Identity=34.9264705882353, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322667, Length=198, Percent_Identity=39.8989898989899, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=46.4968152866242, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=43.620178041543, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24651458, Length=352, Percent_Identity=34.6590909090909, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24651456, Length=352, Percent_Identity=34.6590909090909, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI281362873, Length=352, Percent_Identity=34.6590909090909, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24651454, Length=352, Percent_Identity=34.6590909090909, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24651462, Length=371, Percent_Identity=33.1536388140162, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24651464, Length=371, Percent_Identity=33.1536388140162, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45552010, Length=371, Percent_Identity=33.1536388140162, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35070; Mature: 34939

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEYFDPKLKIFSLNSNRELAEEIAKEVGIELGKSSVTHFSDGEIQINIEESIRGCHVY
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCHHCCCCCCCEECCCEEEEEEHHCCCCEEEE
VIQSTSNPVNQNLMELLIMIDALKRASAATINIVMPYYGYARQDRKARSREPITAKLVAN
EEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHH
LIETAGATRMITLDMHAPQIQGFFDIPIDHLNAVRLLSDYFSERHLGDDLVVVSPDHGGV
HHHHCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCH
TRARKMADRLKAPIAIIDKRRPRPNVAEVMNIVGNVEGKVCIIIDDIIDTAGTITLAAKA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHHHH
LREAGATKVYACCSHPVLSGPAMKRIEESPIEKLVVTNSIALPEEKWIDKMEQLSVAALL
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
GEAIVRVHENASVSSLFE
HHHHHHHCCCCCHHHHCC
>Mature Secondary Structure 
SNEYFDPKLKIFSLNSNRELAEEIAKEVGIELGKSSVTHFSDGEIQINIEESIRGCHVY
CCCCCCCCEEEEEECCCHHHHHHHHHHHCHHCCCCCCCEECCCEEEEEEHHCCCCEEEE
VIQSTSNPVNQNLMELLIMIDALKRASAATINIVMPYYGYARQDRKARSREPITAKLVAN
EEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHH
LIETAGATRMITLDMHAPQIQGFFDIPIDHLNAVRLLSDYFSERHLGDDLVVVSPDHGGV
HHHHCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCH
TRARKMADRLKAPIAIIDKRRPRPNVAEVMNIVGNVEGKVCIIIDDIIDTAGTITLAAKA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHHHH
LREAGATKVYACCSHPVLSGPAMKRIEESPIEKLVVTNSIALPEEKWIDKMEQLSVAALL
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
GEAIVRVHENASVSSLFE
HHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]