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The map label for this gene is glmU

Identifier: 226222827

GI number: 226222827

Start: 197902

End: 199275

Strand: Direct

Name: glmU

Synonym: Lm4b_00195

Alternate gene names: 226222827

Gene position: 197902-199275 (Clockwise)

Preceding gene: 226222826

Following gene: 226222828

Centisome position: 6.79

GC content: 40.25

Gene sequence:

>1374_bases
ATGTCAAAACGATATGCTGTAGTGCTTGCTGCTGGCCAAGGCACACGGATGAAATCAAAACTTTACAAAGTATTGCATCC
TGTTTGTGGAAAACCAATGGTCGAACATGTAGTAGATCAAATTTCGACGCTTAATGTTGATAAAGTGGTTACAATTGTAG
GTCACGGAGCTGAGAAAGTACAAGAGCATTTAGCTGGTAAAAGCGAGTTTGTAAAGCAAGAAGAGCAACTCGGAACGGCG
CATGCAGTACTGCAAGCAAAAGCGGAACTTGCAGGTAAAGATGGTGTGACGCTCGTTGTTTGTGGGGACACGCCTTTAAT
CGAAGCTAGTACGATGGAAGCTTTACTGAAATATCACCATGAAAAACGCGCAAAAGCAACTATTCTTACAACGGTTATTG
AAGATCCTACAGGTTATGGTCGTATTATTCGTGATGACCTAGGCATTGTAGAAAAAATCGTAGAACATAAAGATGCAACA
GAAAAAGAACAACGTATTTCGGAAATTAACACTGGGACATATTGCTTTGATAATAAAGCTCTTTTTGAAGCGTTAGAGAA
TGTTTCTAACGATAATGTGCAAGGAGAATATTACTTACCAGATGTTATTAAGATTTTAAAAGATTCGGATGAAGTCGTTG
CGGCTTACAGAATGGAGTCTTTTGAAGAGTCACTTGGAGTGAACGACCGAATTGCCCTAGCTGAAGCATCCAGATTAATG
CAACGTCGAATTAACGAAAATCATATGCGTAATGGAGTAACGCTTGTTAATCCTGAAAACACGTATATTGATATAGATGT
AAAAATTGGTCAAGATACAGTAATTGAACCAGGAGTTATGCTTCGTGGGGAAACTGTAATCGGTGATGATTGCGTGGTTA
CAAGCGGCTCGGAAATCGTAAACAGTGTTATCGGTGAACGAGTACACGTCAAAACTTCTTCTATTTTCGAAAGTAAAGTT
GGCGATGACGTTCAAATTGGCCCATATGCGCACCTAAGACCAGAATCTGACATTCACGATCATGTGAAAATCGGGAACTA
CGTCGAAACGAAAAAAGCAGTGGTTGGTGAAGGAACTAAATTACCACATTTCATTTACATGGGAGATGCGGAAATTGGTA
AAAACGTCAATGTTGGTTGCGGAAGTATTGCTGTTAATTACGACGGCAAAAACAAAGCGAAAACCATCATTGGGGATAAT
GTCTTTGTTGGCTGTAATTCAAACCTGATTGCACCTGTCAAAGTGGGAGATCGAGCTTTCATAGCTGCTGGTTCCACGAT
TACAAAAGATGTTCCTGACGACGCGCTAGGCATTGCTCGTGCGAAACAAGACAATAAACTTGGTTATGCGAAACATTTGA
ATCACGGTAAATAA

Upstream 100 bases:

>100_bases
AAGTAGGTAAGGTTTTTTATTTGGGCGCATTCTTGAAAAAATGTAAATTTTACGTTATCATTCATAGTGGATGAATAGTG
AATATTGGAGGTTCTATATA

Downstream 100 bases:

>100_bases
TTTCCATTTGGGAATTTTAAAATTTTAATAAAAAATAGTGGAGGCTGATTATGTCAAACGAGTATTTTGATCCAAAGTTG
AAGATTTTCTCGCTAAATTC

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSKRYAVVLAAGQGTRMKSKLYKVLHPVCGKPMVEHVVDQISTLNVDKVVTIVGHGAEKVQEHLAGKSEFVKQEEQLGTA
HAVLQAKAELAGKDGVTLVVCGDTPLIEASTMEALLKYHHEKRAKATILTTVIEDPTGYGRIIRDDLGIVEKIVEHKDAT
EKEQRISEINTGTYCFDNKALFEALENVSNDNVQGEYYLPDVIKILKDSDEVVAAYRMESFEESLGVNDRIALAEASRLM
QRRINENHMRNGVTLVNPENTYIDIDVKIGQDTVIEPGVMLRGETVIGDDCVVTSGSEIVNSVIGERVHVKTSSIFESKV
GDDVQIGPYAHLRPESDIHDHVKIGNYVETKKAVVGEGTKLPHFIYMGDAEIGKNVNVGCGSIAVNYDGKNKAKTIIGDN
VFVGCNSNLIAPVKVGDRAFIAAGSTITKDVPDDALGIARAKQDNKLGYAKHLNHGK

Sequences:

>Translated_457_residues
MSKRYAVVLAAGQGTRMKSKLYKVLHPVCGKPMVEHVVDQISTLNVDKVVTIVGHGAEKVQEHLAGKSEFVKQEEQLGTA
HAVLQAKAELAGKDGVTLVVCGDTPLIEASTMEALLKYHHEKRAKATILTTVIEDPTGYGRIIRDDLGIVEKIVEHKDAT
EKEQRISEINTGTYCFDNKALFEALENVSNDNVQGEYYLPDVIKILKDSDEVVAAYRMESFEESLGVNDRIALAEASRLM
QRRINENHMRNGVTLVNPENTYIDIDVKIGQDTVIEPGVMLRGETVIGDDCVVTSGSEIVNSVIGERVHVKTSSIFESKV
GDDVQIGPYAHLRPESDIHDHVKIGNYVETKKAVVGEGTKLPHFIYMGDAEIGKNVNVGCGSIAVNYDGKNKAKTIIGDN
VFVGCNSNLIAPVKVGDRAFIAAGSTITKDVPDDALGIARAKQDNKLGYAKHLNHGK
>Mature_456_residues
SKRYAVVLAAGQGTRMKSKLYKVLHPVCGKPMVEHVVDQISTLNVDKVVTIVGHGAEKVQEHLAGKSEFVKQEEQLGTAH
AVLQAKAELAGKDGVTLVVCGDTPLIEASTMEALLKYHHEKRAKATILTTVIEDPTGYGRIIRDDLGIVEKIVEHKDATE
KEQRISEINTGTYCFDNKALFEALENVSNDNVQGEYYLPDVIKILKDSDEVVAAYRMESFEESLGVNDRIALAEASRLMQ
RRINENHMRNGVTLVNPENTYIDIDVKIGQDTVIEPGVMLRGETVIGDDCVVTSGSEIVNSVIGERVHVKTSSIFESKVG
DDVQIGPYAHLRPESDIHDHVKIGNYVETKKAVVGEGTKLPHFIYMGDAEIGKNVNVGCGSIAVNYDGKNKAKTIIGDNV
FVGCNSNLIAPVKVGDRAFIAAGSTITKDVPDDALGIARAKQDNKLGYAKHLNHGK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc [H]

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1790168, Length=447, Percent_Identity=40.4921700223714, Blast_Score=324, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI133931050, Length=346, Percent_Identity=25.4335260115607, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=373, Percent_Identity=23.0563002680965, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21355443, Length=326, Percent_Identity=24.5398773006135, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644084, Length=326, Percent_Identity=24.5398773006135, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 49809; Mature: 49678

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRYAVVLAAGQGTRMKSKLYKVLHPVCGKPMVEHVVDQISTLNVDKVVTIVGHGAEKV
CCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEEEEECCCHHHH
QEHLAGKSEFVKQEEQLGTAHAVLQAKAELAGKDGVTLVVCGDTPLIEASTMEALLKYHH
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
EKRAKATILTTVIEDPTGYGRIIRDDLGIVEKIVEHKDATEKEQRISEINTGTYCFDNKA
HHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCHH
LFEALENVSNDNVQGEYYLPDVIKILKDSDEVVAAYRMESFEESLGVNDRIALAEASRLM
HHHHHHCCCCCCCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
QRRINENHMRNGVTLVNPENTYIDIDVKIGQDTVIEPGVMLRGETVIGDDCVVTSGSEIV
HHHHCHHHHHCCEEEECCCCCEEEEEEEECCCCEECCCEEEECCEEECCCEEEECCHHHH
NSVIGERVHVKTSSIFESKVGDDVQIGPYAHLRPESDIHDHVKIGNYVETKKAVVGEGTK
HHHHCCEEEEEHHHHHHHHCCCCEEECCEECCCCCCCCHHHHHCCCCHHHHHHHHCCCCC
LPHFIYMGDAEIGKNVNVGCGSIAVNYDGKNKAKTIIGDNVFVGCNSNLIAPVKVGDRAF
CCEEEEECCHHHCCCCCCCCCEEEEEECCCCCCEEEECCEEEEECCCCEEEEEEECCEEE
IAAGSTITKDVPDDALGIARAKQDNKLGYAKHLNHGK
EEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
SKRYAVVLAAGQGTRMKSKLYKVLHPVCGKPMVEHVVDQISTLNVDKVVTIVGHGAEKV
CCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEEEEECCCHHHH
QEHLAGKSEFVKQEEQLGTAHAVLQAKAELAGKDGVTLVVCGDTPLIEASTMEALLKYHH
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
EKRAKATILTTVIEDPTGYGRIIRDDLGIVEKIVEHKDATEKEQRISEINTGTYCFDNKA
HHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCHH
LFEALENVSNDNVQGEYYLPDVIKILKDSDEVVAAYRMESFEESLGVNDRIALAEASRLM
HHHHHHCCCCCCCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
QRRINENHMRNGVTLVNPENTYIDIDVKIGQDTVIEPGVMLRGETVIGDDCVVTSGSEIV
HHHHCHHHHHCCEEEECCCCCEEEEEEEECCCCEECCCEEEECCEEECCCEEEECCHHHH
NSVIGERVHVKTSSIFESKVGDDVQIGPYAHLRPESDIHDHVKIGNYVETKKAVVGEGTK
HHHHCCEEEEEHHHHHHHHCCCCEEECCEECCCCCCCCHHHHHCCCCHHHHHHHHCCCCC
LPHFIYMGDAEIGKNVNVGCGSIAVNYDGKNKAKTIIGDNVFVGCNSNLIAPVKVGDRAF
CCEEEEECCHHHCCCCCCCCCEEEEEECCCCCCEEEECCEEEEECCCCEEEEEEECCEEE
IAAGSTITKDVPDDALGIARAKQDNKLGYAKHLNHGK
EEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]