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The map label for this gene is hly

Identifier: 226222831

GI number: 226222831

Start: 202508

End: 204097

Strand: Direct

Name: hly

Synonym: Lm4b_00200

Alternate gene names: 226222831

Gene position: 202508-204097 (Clockwise)

Preceding gene: 226222828

Following gene: 226222832

Centisome position: 6.95

GC content: 35.97

Gene sequence:

>1590_bases
ATGAAAAAAATAATGCTAGTTTTTATTACACTTATATTAGTTAGTCTACCAATTGCGCAACAAACTGAAGCAAAGGATGC
ATCTGCATTCCATAAAGAAAATTTAATTTCATCCATGGCACCACCAGCATCTCCGCCTGCAAGTCCTAAGACGCCAATCG
AAAAGAAACACGCGGATGAAATCGATAAGTATATACAAGGATTGGATTACAATAAAAACAATGTATTAGTATACCACGGA
GATGCAGTGACAAATGTGCCGCCAAGAAAAGGTTATAAAGATGGAAATGAATATATCGTTGTGGAGAAAAAGAAGAAATC
CATCAATCAAAATAATGCAGATATCCAAGTTGTGAATGCAATTTCGAGCCTAACATATCCAGGTGCTCTCGTGAAAGCGA
ATTCGGAATTAGTAGAAAATCAACCCGATGTTCTTCCTGTCAAACGTGATTCATTAACACTTAGCATTGATTTGCCAGGA
ATGACTAATCAAGACAATAAAATTGTTGTAAAAAATGCTACTAAATCGAACGTTAACAACGCAGTAAATACATTAGTGGA
AAGATGGAATGAAAAATATGCTCAAGCTTATCCAAATGTAAGTGCAAAAATTGATTATGATGACGAAATGGCTTACAGTG
AATCGCAATTAATTGCAAAATTTGGTACGGCATTTAAAGCTGTAAATAATAGCTTGAATGTAAACTTCGGCGCAATCAGT
GAAGGGAAAATGCAAGAAGAAGTCATTAGTTTTAAACAAATTTACTATAACGTGAATGTTAATGAACCTACAAGACCTTC
CAGATTTTTCGGCAAAGCTGTTACTAAAGAGCAGTTGCAAGCGCTTGGAGTGAATGCAGAAAATCCTCCTGCATATATCT
CAAGTGTGGCATATGGCCGTCAAGTTTATTTGAAATTATCAACTAATTCCCATAGTACTAAAGTAAAAGCTGCTTTTGAT
GCTGCCGTAAGTGGGAAATCTGTCTCAGGTGATGTAGAACTGACAAATATCATCAAAAATTCTTCCTTCAAAGCCGTAAT
TTACGGTGGCTCCGCAAAAGATGAAGTTCAAATCATCGACGGTAACCTCGGAGACTTACGAGATATTTTGAAAAAAGGTG
CTACTTTTAACCGGGAAACACCAGGAGTTCCCATTGCCTATACAACAAACTTCTTAAAAGACAATGAATTAGCTGTTATT
AAAAACAACTCAGAATATATTGAAACAACTTCAAAAGCTTATACAGATGGAAAAATCAACATCGATCACTCTGGAGGATA
CGTTGCTCAATTCAACATCTCTTGGGATGAAATAAATTATGATCCTGAAGGTAACGAAATTGTTCAACATAAAAACTGGA
GCGAAAACAATAAAAGTAAGCTAGCTCATTTCACATCGTCCATCTATTTGCCAGGTAACGCAAGAAATATTAATGTTTAC
GCTAAAGAATGCACTGGTTTAGCTTGGGAATGGTGGAGAACGGTAATTGATGACCGGAACCTACCGCTTGTGAAAAATAG
AAATATCTCCATCTGGGGCACTACACTTTATCCGAAATATAGTAATAGTGTAGATAATCCAATCGAATAA

Upstream 100 bases:

>100_bases
AGAAGCGAATTTCGCCAATATTATAATTATCAAAAGAAAGGAGGGGCTAAACGGTATTTGGCATTATTAGGTTAAAAAAT
GTAGAAGGAGAGTGAAACCC

Downstream 100 bases:

>100_bases
TTTTAAAAATTAATAAAAAAATTAAGAATAAAACCGCTTAACACACACACGAAAAATAAGCTTGTTTTGCACTTTTCATA
AATTATTTTATGAAGAATGT

Product: listeriolysin O precursor

Products: NA

Alternate protein names: LLO; Thiol-activated cytolysin [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKKIMLVFITLILVSLPIAQQTEAKDASAFHKENLISSMAPPASPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHG
DAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPG
MTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAIS
EGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFD
AAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVI
KNNSEYIETTSKAYTDGKINIDHSGGYVAQFNISWDEINYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVY
AKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNSVDNPIE

Sequences:

>Translated_529_residues
MKKIMLVFITLILVSLPIAQQTEAKDASAFHKENLISSMAPPASPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHG
DAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPG
MTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAIS
EGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFD
AAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVI
KNNSEYIETTSKAYTDGKINIDHSGGYVAQFNISWDEINYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVY
AKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNSVDNPIE
>Mature_529_residues
MKKIMLVFITLILVSLPIAQQTEAKDASAFHKENLISSMAPPASPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHG
DAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPG
MTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAIS
EGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFD
AAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVI
KNNSEYIETTSKAYTDGKINIDHSGGYVAQFNISWDEINYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVY
AKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNSVDNPIE

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host membrane; Multi-pass membrane protein (By similarity). Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host membrane and forms pores formed by transmembrane beta-s

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 58711; Mature: 58711

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00481 THIOL_CYTOLYSINS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIMLVFITLILVSLPIAQQTEAKDASAFHKENLISSMAPPASPPASPKTPIEKKHADE
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
IDKYIQGLDYNKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNA
HHHHHHCCCCCCCCEEEEECCEECCCCCCCCCCCCCCEEEEECHHHCCCCCCCCEEEEEH
ISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNN
HHHCCCCCEEEECCHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEECCCHHHHHH
AVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAIS
HHHHHHHHHHHHHHHHCCCCCEEECCCCCHHCCHHHHHHHHHHHHHHHCCCCEEEECCCC
EGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGR
CCHHHHHHHCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCE
QVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIID
EEEEEEECCCCCEEEEEEHHHHCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEE
GNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTDGKIN
CCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCHHEEECCCEECCCEEE
IDHSGGYVAQFNISWDEINYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVY
EECCCCEEEEEEEEHHHCCCCCCCCEEEECCCCCCCCHHHHEEEEEEEEECCCCCEEEEE
AKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNSVDNPIE
ECCCCCCHHHHHHHHHCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKKIMLVFITLILVSLPIAQQTEAKDASAFHKENLISSMAPPASPPASPKTPIEKKHADE
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
IDKYIQGLDYNKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNA
HHHHHHCCCCCCCCEEEEECCEECCCCCCCCCCCCCCEEEEECHHHCCCCCCCCEEEEEH
ISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNN
HHHCCCCCEEEECCHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEECCCHHHHHH
AVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAIS
HHHHHHHHHHHHHHHHCCCCCEEECCCCCHHCCHHHHHHHHHHHHHHHCCCCEEEECCCC
EGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGR
CCHHHHHHHCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCE
QVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIID
EEEEEEECCCCCEEEEEEHHHHCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEE
GNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTDGKIN
CCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCHHEEECCCEECCCEEE
IDHSGGYVAQFNISWDEINYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVY
EECCCCEEEEEEEEHHHCCCCCCCCEEEECCCCCCCCHHHHEEEEEEEEECCCCCEEEEE
AKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNSVDNPIE
ECCCCCCHHHHHHHHHCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA