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The map label for this gene is leuA [H]

Identifier: 225859052

GI number: 225859052

Start: 1225628

End: 1226062

Strand: Reverse

Name: leuA [H]

Synonym: SP70585_1321

Alternate gene names: 225859052

Gene position: 1226062-1225628 (Counterclockwise)

Preceding gene: 225859053

Following gene: 225859051

Centisome position: 56.12

GC content: 41.38

Gene sequence:

>435_bases
TTGGTAGCTGGAACCATGGTTGAAAATCCAGAAGGCTTCCACTTTGATGATTTACAACTTCAAACTCATGCAGATAATGA
CATTGAAGCGCTCGTTAGCCTAGCCAATATGGATGGTGAGAAAGTCGAATTTAATGCGACAGGGCAAGGTTCCGTTGAAG
CAATCTTTAATGCTATCGATAAGTTCTTTAACCAATCTGTTCGTTTGGTGTCCTACACTATCGATGCGGTAACAGATGGA
ATCGATACCCAGGATCGGGTTTTGGTCACTGTTGAAAACAGAGATACAGAAACCATCTTTAATGCAGCAGGGCTTGATTT
TGATGTGTTGAAGGCTTCTGCTATTGTCTATATAAACGCTAATACCTTTGTTCAAAAAGAGAATGCAGGTGAGATGGGAC
GCAGTGTTTCTTACCACGATATGCCTAGTGTGTAA

Upstream 100 bases:

>100_bases
GGCCCTAGATTTTACAGAAGAGGATATCAAACCACTCTTTGCTAAGTTCAAGGCACTGGCCGATAAGAAGTAAGAAATCA
CAGATGCAGATACTCGAGCT

Downstream 100 bases:

>100_bases
AGGAGAAGGCTATGGCAAAGAAAATAGTAGCTCTAGCAGGAGACGGAATTGGCCCAGAAATCATGGAGGTTGGTTTAGAA
GTTCTGGAGGCTCTAGCTGA

Product: 2-isopropylmalate synthase (Alpha-isopropylmalatesynthase) (Alpha-IPM synthetase)

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDG
IDTQDRVLVTVENRDTETIFNAAGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV

Sequences:

>Translated_144_residues
MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDG
IDTQDRVLVTVENRDTETIFNAAGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV
>Mature_144_residues
MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDG
IDTQDRVLVTVENRDTETIFNAAGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 15771; Mature: 15771

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAID
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
KFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASAIVYINA
HHHCCCEEEEEEEEEHHHCCCCCCCEEEEEEECCCCCCEECCCCCCCEEECCEEEEEECC
NTFVQKENAGEMGRSVSYHDMPSV
CEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAID
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
KFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASAIVYINA
HHHCCCEEEEEEEEEHHHCCCCCCCEEEEEEECCCCCCEECCCCCCCEEECCEEEEEECC
NTFVQKENAGEMGRSVSYHDMPSV
CEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]