Click here to switch to the map view.

The map label for this gene is leuB

Identifier: 225859051

GI number: 225859051

Start: 1224579

End: 1225616

Strand: Reverse

Name: leuB

Synonym: SP70585_1320

Alternate gene names: 225859051

Gene position: 1225616-1224579 (Counterclockwise)

Preceding gene: 225859052

Following gene: 225859050

Centisome position: 56.1

GC content: 43.93

Gene sequence:

>1038_bases
ATGGCAAAGAAAATAGTAGCTCTAGCAGGAGACGGAATTGGCCCAGAAATCATGGAGGTTGGTTTAGAAGTTCTGGAGGC
TCTAGCTGAAAAAACAGGTTTTGACTATGAGATTGACAGACGACCGTTCGGAGGTGCAGATATTGATGCAGCAGGACCTC
CCTTACCTGATGAAACCCTTAAGGCAAGTAGGGAAGCAGATGCTATCCTACTAGCAGCTATCGGTAGTCCTCAGTATGAT
GGAGCAGTGGTTCGCCCTGAACAGGGCCTGATGGCTCTCCGTAAGGAACTCAATCTTTACGCTAATATTCGTCCTGTAAA
AATCTTTGACAGTCTCAAGCATTTGTCACCACTCAAACTGGAACGAATTGCTGGTGTAGACTTTGTCGTGGTGCGTGAAT
TGACAGGCGGGATTTACTTTGGAGATCATATTCTTGAAGAGCGCAATGCGCGTGATATCAACGACTATAGCTATGAGGAA
GTGGAGCGGATTATTCGCAAAGCCTTTGAAATTGCAAGAAATCGCAGAAAAATCCTTACTAGTATCGATAAGCAAAATGT
TCTAGCGACCTCAAAACTCTGGCGGAAAGTAGCTGAGGAAGTCGCACAGGATTTCCCAGATGTAACCTTGGAACACCAGC
TGGTAGACTCAGCTGCTATGCTTATGATTACCAATCCTGCTAAGTTTGATGTTATTGTAACGGAGAATCTTTTTGGAGAT
ATTTTATCTGATGAATCAAGCGTCTTATCTGGTACACTTGAGGTTATGCCATCAGCCAGTCATTCTGAAAATGGACCAAG
TCTCTATGAACCTATTCACGGTTCAGCACCTGATATTGCAGGTCAAGGAATTGCCAATCCTACTTCCATGATTTTATCAG
TTGCCATGATGTTGAGAGATAGTTTCGGACGTTATGAGGATGCAGAGCGTATCAAACGTGCTGTTGAGACAAGTCTGGCG
GCAGGAATTTTAACGAGAGATATAGGAGGTCAGGCTTCAACAAAGGAAATGACGGAAGCTATTATTGCAAGGTTATGA

Upstream 100 bases:

>100_bases
TCTATATAAACGCTAATACCTTTGTTCAAAAAGAGAATGCAGGTGAGATGGGACGCAGTGTTTCTTACCACGATATGCCT
AGTGTGTAAAGGAGAAGGCT

Downstream 100 bases:

>100_bases
AGTTAGACGAAAAAATTACTCTAGTCCTTTTGATTTGGAATGTCATCATTTTCTTGATTTATGGCATTGACAAATTCAAG
GCAAGAAGAAGAGCTTGGCG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLAAIGSPQYD
GAVVRPEQGLMALRKELNLYANIRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLA
AGILTRDIGGQASTKEMTEAIIARL

Sequences:

>Translated_345_residues
MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLAAIGSPQYD
GAVVRPEQGLMALRKELNLYANIRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLA
AGILTRDIGGQASTKEMTEAIIARL
>Mature_344_residues
AKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLAAIGSPQYDG
AVVRPEQGLMALRKELNLYANIRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEEV
ERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGDI
LSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLAA
GILTRDIGGQASTKEMTEAIIARL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=33.4293948126801, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI28178816, Length=349, Percent_Identity=28.6532951289398, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI28178821, Length=349, Percent_Identity=27.7936962750716, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4758582, Length=260, Percent_Identity=29.6153846153846, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI28178819, Length=186, Percent_Identity=29.5698924731183, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI28178838, Length=233, Percent_Identity=29.1845493562232, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=45.4545454545455, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788101, Length=354, Percent_Identity=36.4406779661017, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787381, Length=375, Percent_Identity=28, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=29.2957746478873, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=349, Percent_Identity=30.0859598853868, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=348, Percent_Identity=27.2988505747126, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=352, Percent_Identity=46.875, Blast_Score=296, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=37.4301675977654, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=346, Percent_Identity=34.971098265896, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=263, Percent_Identity=36.5019011406844, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=35.4285714285714, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=35.4285714285714, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=30.7909604519774, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281362242, Length=351, Percent_Identity=29.3447293447293, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24648872, Length=351, Percent_Identity=29.3447293447293, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078635, Length=352, Percent_Identity=29.5454545454545, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078637, Length=352, Percent_Identity=29.5454545454545, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078633, Length=352, Percent_Identity=29.5454545454545, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650122, Length=352, Percent_Identity=29.5454545454545, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078639, Length=349, Percent_Identity=30.0859598853868, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=24.1279069767442, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37705; Mature: 37573

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00230 MAP1B_NEURAXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHH
KASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYANIRPVKIFDSLKHLSPLKL
HHCCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
ERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEEVERIIRKAFEIARNRRKILT
HHHCCCHHEEHHHHHCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCEEEEEEEHHHHHH
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRD
HHCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFGRYEDAERIKRAVETSLAAGILTRDIGGQASTKEMTEAIIARL
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHH
KASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYANIRPVKIFDSLKHLSPLKL
HHCCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
ERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEEVERIIRKAFEIARNRRKILT
HHHCCCHHEEHHHHHCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCEEEEEEEHHHHHH
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRD
HHCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFGRYEDAERIKRAVETSLAAGILTRDIGGQASTKEMTEAIIARL
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11544234 [H]