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The map label for this gene is leuA [H]

Identifier: 225859053

GI number: 225859053

Start: 1226090

End: 1226722

Strand: Reverse

Name: leuA [H]

Synonym: SP70585_1322

Alternate gene names: 225859053

Gene position: 1226722-1226090 (Counterclockwise)

Preceding gene: 225859054

Following gene: 225859052

Centisome position: 56.15

GC content: 41.71

Gene sequence:

>633_bases
ATGAACTTTCAATTAGCTGAATATTCTTTACTGAAGAAATTTTCAGAAAATATAGGATTTACCACACCAGAGGAATGTGG
TGCCATCTTCAAATACCTGATTGAGAATGTCAAGACGGATCGTCAGATCATCTATTCGCCTCACTGTCATGATGACCTCG
GAATGGCAGTGGCAAATAGCCTTGCTGCTGTCAAGAATGGTGCAGGACGTGTTGAAGGGACTATCAATGGTATTAGGGAG
CGAGCTGAAAATGCTGCTTTGGAAGAAATTGCAGTGGCTCTCAATATTCGCCAAGATTACTACCAAGTAGAAACCAGTAT
TGTCCTAAATGAGACCATCAATACGTCAGAAATGGTTTCTCGCTTCTCTGGTATTCCAGTTCCTAAAAACAAAGCCGTCG
TTGGTGGCAATACCTTCTCCCACGAATCTGGTATTCACCAAGATGGAGTCCTTAAAAATCCTCTCACTTATGAGATCATC
ACACCTGAATTGGTTGGTGTTAAGATTCCGCTTGGAAAATTATCTGGTCGCCATGCTTTTGTTGAGAAACTGAGAGAATT
GGCCCTAGATTTTACAGAAGAGGATATCAAACCACTCTTTGCTAAGTTCAAGGCACTGGCCGATAAGAAGTAA

Upstream 100 bases:

>100_bases
TGTGTCCCTTGATGATACTTTATCAGATGAAGAAGTACTGGAATTGATAGAAAAAAGTTGGAACTTAACCTCTAAAAAAT
GAAATATTTTAATAATTTTC

Downstream 100 bases:

>100_bases
GAAATCACAGATGCAGATACTCGAGCTTTGGTAGCTGGAACCATGGTTGAAAATCCAGAAGGCTTCCACTTTGATGATTT
ACAACTTCAAACTCATGCAG

Product: 2-isopropylmalate synthase (Alpha-isopropylmalatesynthase) (Alpha-IPM synthetase)

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNFQLAEYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQIIYSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRE
RAENAALEEIAVALNIRQDYYQVETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK

Sequences:

>Translated_210_residues
MNFQLAEYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQIIYSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRE
RAENAALEEIAVALNIRQDYYQVETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK
>Mature_210_residues
MNFQLAEYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQIIYSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRE
RAENAALEEIAVALNIRQDYYQVETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=198, Percent_Identity=45.959595959596, Blast_Score=174, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6320019, Length=198, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320071, Length=198, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 23225; Mature: 23225

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQLAEYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQIIYSPHCHDDLGMAVANS
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
LAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQVETSIVLNETINTSEMVS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHH
RFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEIITPELVGVKIPLGKLSGRHAF
HHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCHHEEEEECHHHCCCHHHH
VEKLRELALDFTEEDIKPLFAKFKALADKK
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFQLAEYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQIIYSPHCHDDLGMAVANS
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
LAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQVETSIVLNETINTSEMVS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHH
RFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEIITPELVGVKIPLGKLSGRHAF
HHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCHHEEEEECHHHCCCHHHH
VEKLRELALDFTEEDIKPLFAKFKALADKK
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA