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The map label for this gene is queD [H]

Identifier: 225853409

GI number: 225853409

Start: 1920773

End: 1921138

Strand: Direct

Name: queD [H]

Synonym: BMEA_A2030

Alternate gene names: 225853409

Gene position: 1920773-1921138 (Clockwise)

Preceding gene: 225853408

Following gene: 225853410

Centisome position: 90.36

GC content: 56.28

Gene sequence:

>366_bases
ATGTTTCGCATCACCAAGGAATTCCACTTTTCCGCCTCGCACCAGCTCACCGGCCTGCCTGCCGATCACCAATGCGCGCG
CTTGCATGGCCATAACTATATCGTCGAGGTGGAACTTTCCGCGCCCGATCTCGACACCCATGGTTTCGTGCGCGATTATC
ACGAGCTGGCTGCGCTCAAGCATTATATTGATGATACTTTCGATCATCGCCATCTCAACGATGTGCTGGGCCATGAACAG
ACCACGGCGGAACGGCTGGCAAAACATTTCTTCGACTGGTGCCATGCGCGATGGCCGGAAATCTCCGCAGTGCGGGTCAG
CGAAACACCGAAGACCTGGGCGGAATACCGGCCCGGAAAACCATGA

Upstream 100 bases:

>100_bases
TGGAGCGGCGCGAGGCGTTTCATCTTGCAGGAATAGACGACCCGACCAGCTATGAGGATGCCGACTTCTGGCGCGCCACC
ACGCAAAAAAGGAACGCCTG

Downstream 100 bases:

>100_bases
TGAAACCCGCAATGAAACCTGTAATGAAACCCGGCCCCGAAATCCGCATCAGCGAGATTTTCGGCCCGACCATTCAAGGT
GAGGGCGTCCTGATCGGCGA

Product: queuosine biosynthesis protein QueD

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 121; Mature: 121

Protein sequence:

>121_residues
MFRITKEFHFSASHQLTGLPADHQCARLHGHNYIVEVELSAPDLDTHGFVRDYHELAALKHYIDDTFDHRHLNDVLGHEQ
TTAERLAKHFFDWCHARWPEISAVRVSETPKTWAEYRPGKP

Sequences:

>Translated_121_residues
MFRITKEFHFSASHQLTGLPADHQCARLHGHNYIVEVELSAPDLDTHGFVRDYHELAALKHYIDDTFDHRHLNDVLGHEQ
TTAERLAKHFFDWCHARWPEISAVRVSETPKTWAEYRPGKP
>Mature_121_residues
MFRITKEFHFSASHQLTGLPADHQCARLHGHNYIVEVELSAPDLDTHGFVRDYHELAALKHYIDDTFDHRHLNDVLGHEQ
TTAERLAKHFFDWCHARWPEISAVRVSETPKTWAEYRPGKP

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=113, Percent_Identity=39.8230088495575, Blast_Score=80, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 14154; Mature: 14154

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRITKEFHFSASHQLTGLPADHQCARLHGHNYIVEVELSAPDLDTHGFVRDYHELAALK
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
HYIDDTFDHRHLNDVLGHEQTTAERLAKHFFDWCHARWPEISAVRVSETPKTWAEYRPGK
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCC
P
C
>Mature Secondary Structure
MFRITKEFHFSASHQLTGLPADHQCARLHGHNYIVEVELSAPDLDTHGFVRDYHELAALK
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
HYIDDTFDHRHLNDVLGHEQTTAERLAKHFFDWCHARWPEISAVRVSETPKTWAEYRPGK
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]