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The map label for this gene is queE [H]

Identifier: 225853410

GI number: 225853410

Start: 1921138

End: 1921893

Strand: Direct

Name: queE [H]

Synonym: BMEA_A2031

Alternate gene names: 225853410

Gene position: 1921138-1921893 (Clockwise)

Preceding gene: 225853409

Following gene: 225853414

Centisome position: 90.38

GC content: 58.33

Gene sequence:

>756_bases
ATGAAACCCGCAATGAAACCTGTAATGAAACCCGGCCCCGAAATCCGCATCAGCGAGATTTTCGGCCCGACCATTCAAGG
TGAGGGCGTCCTGATCGGCGAGCCGACAGTCTTCGTGCGCACTGGCGGCTGCGACTATCGTTGCGCATGGTGCGACAGCC
TGCACGCGGTGGAAAGCCGCTTTCGTCATGAATGGAAGCCGATGAGCGTCGAAGCCATATGGCATGAAGTGACGGCACTT
TCCCAAAAAAGGCCATTAACGGTGTCACTTTCAGGCGGCAATCCAGCCATCCAGCCATTGGGGGCGCTGATCAAACATGG
CAAGGCCGAAGGCTATCGCTTTGCGCTTGAAACACAAGGGTCAGTGGCAAAGGACTGGTTTTCCCGGCTGGACACATTGG
TTTTGAGCCCCAAACCGCCTTCAAGCGCCATGGAAACCGATTGGGACATGCTTGCCGCCTGTATCGAGGCGGCAGGCACT
GGGCCGCGCACCGTGCTGAAATTCGTGATTTTCGATGAGGCCGACTATGCCTATGCAAGGACGGTTTCTGCGCGCCATCC
GCAATTGCCGGTCTATGTCCAGCCCGGCAATCATACCCCGCCACCGCCCGACGCCGACGATGCCGCAATCGATATCGACG
GCATCATGCGACGCATGGAATGGCTGGTGGACAAGGTGATAGCCGACGGCTGGTTCGAGGCGCATGTTCTGCCGCAACTG
CACGTGCTGATCTGGGGCAACAGACGCGGCGTCTAA

Upstream 100 bases:

>100_bases
ATTTCTTCGACTGGTGCCATGCGCGATGGCCGGAAATCTCCGCAGTGCGGGTCAGCGAAACACCGAAGACCTGGGCGGAA
TACCGGCCCGGAAAACCATG

Downstream 100 bases:

>100_bases
AGCGGTTCCGGCTAGAGCGCACCCCGAAAAGTGTGAAACGGTTTTCGGAAAAGACGCGTGTCAAAACAAAGGATTAGAGC
GCCGATCTGATTCAATCAGA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKPAMKPVMKPGPEIRISEIFGPTIQGEGVLIGEPTVFVRTGGCDYRCAWCDSLHAVESRFRHEWKPMSVEAIWHEVTAL
SQKRPLTVSLSGGNPAIQPLGALIKHGKAEGYRFALETQGSVAKDWFSRLDTLVLSPKPPSSAMETDWDMLAACIEAAGT
GPRTVLKFVIFDEADYAYARTVSARHPQLPVYVQPGNHTPPPPDADDAAIDIDGIMRRMEWLVDKVIADGWFEAHVLPQL
HVLIWGNRRGV

Sequences:

>Translated_251_residues
MKPAMKPVMKPGPEIRISEIFGPTIQGEGVLIGEPTVFVRTGGCDYRCAWCDSLHAVESRFRHEWKPMSVEAIWHEVTAL
SQKRPLTVSLSGGNPAIQPLGALIKHGKAEGYRFALETQGSVAKDWFSRLDTLVLSPKPPSSAMETDWDMLAACIEAAGT
GPRTVLKFVIFDEADYAYARTVSARHPQLPVYVQPGNHTPPPPDADDAAIDIDGIMRRMEWLVDKVIADGWFEAHVLPQL
HVLIWGNRRGV
>Mature_251_residues
MKPAMKPVMKPGPEIRISEIFGPTIQGEGVLIGEPTVFVRTGGCDYRCAWCDSLHAVESRFRHEWKPMSVEAIWHEVTAL
SQKRPLTVSLSGGNPAIQPLGALIKHGKAEGYRFALETQGSVAKDWFSRLDTLVLSPKPPSSAMETDWDMLAACIEAAGT
GPRTVLKFVIFDEADYAYARTVSARHPQLPVYVQPGNHTPPPPDADDAAIDIDGIMRRMEWLVDKVIADGWFEAHVLPQL
HVLIWGNRRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 27761; Mature: 27761

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAMKPVMKPGPEIRISEIFGPTIQGEGVLIGEPTVFVRTGGCDYRCAWCDSLHAVESR
CCCCCCHHHCCCCCEEEEECCCCCCCCCEEEEECCEEEEEECCCCEEEHHHHHHHHHHHH
FRHEWKPMSVEAIWHEVTALSQKRPLTVSLSGGNPAIQPLGALIKHGKAEGYRFALETQG
HHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCC
SVAKDWFSRLDTLVLSPKPPSSAMETDWDMLAACIEAAGTGPRTVLKFVIFDEADYAYAR
CHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHE
TVSARHPQLPVYVQPGNHTPPPPDADDAAIDIDGIMRRMEWLVDKVIADGWFEAHVLPQL
ECCCCCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCEE
HVLIWGNRRGV
EEEEECCCCCC
>Mature Secondary Structure
MKPAMKPVMKPGPEIRISEIFGPTIQGEGVLIGEPTVFVRTGGCDYRCAWCDSLHAVESR
CCCCCCHHHCCCCCEEEEECCCCCCCCCEEEEECCEEEEEECCCCEEEHHHHHHHHHHHH
FRHEWKPMSVEAIWHEVTALSQKRPLTVSLSGGNPAIQPLGALIKHGKAEGYRFALETQG
HHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCC
SVAKDWFSRLDTLVLSPKPPSSAMETDWDMLAACIEAAGTGPRTVLKFVIFDEADYAYAR
CHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHE
TVSARHPQLPVYVQPGNHTPPPPDADDAAIDIDGIMRRMEWLVDKVIADGWFEAHVLPQL
ECCCCCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCEE
HVLIWGNRRGV
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]