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The map label for this gene is prfA [H]

Identifier: 225853308

GI number: 225853308

Start: 1823059

End: 1824138

Strand: Reverse

Name: prfA [H]

Synonym: BMEA_A1923

Alternate gene names: 225853308

Gene position: 1824138-1823059 (Counterclockwise)

Preceding gene: 225853309

Following gene: 225853307

Centisome position: 85.81

GC content: 60.19

Gene sequence:

>1080_bases
ATGATCGCATTGCCCCAGGACCGAATGGATCAGCTTTTGAAGCGGTTCTCGATGATTGAAAGCCAGATGGCCAACAATCC
CGATTCGGATACCTATGTGAAGCTTGCCTCCGAATATTCCGAACTCCAGGACGTGGTGGGCAAAATCCGCGAACTGAGCG
ATGCGCGCATGGAAGCAAGCGACCTTGCCGCGATGCGCGACGATGCTTCGACGGATGCGGAGATGCGCGCTCTGGCGGTG
GAGGAACTGCCGGAGGTGGAAAAGCGCATCGCGGTGCTGGAACAGGATGTGCAAATCCTGCTTCTGCCCAAGGATGCCGC
CGACGACAAGAATGCGATCCTTGAAATTCGCGCCGGTACGGGCGGTCTGGAAGCTGCCCTTTTCGCGGGCGATCTGTTCC
GCATGTATGAACGCTATGCGGCTGAAAAAGGCTGGCGGGTGGAACTTGTTTCGGCCAGCGAGGGCGATGCGGGCGGCTAT
AAGGAAATCATCGCCACGGTTTCGGGCAAGGGGGTCTTCTCAAAGCTGAAGTTTGAATCCGGCGTGCATCGCGTGCAGCG
CGTGCCGGAAACGGAAGCGGGCGGGCGCATCCACACATCGGCAGCCACGGTTGCCGTTCTGCCGGAAGCGGAAGATATCG
ATATTGAAATTCGCAACGAGGATATCCGGATCGATACGATGCGCGCCTCGGGCGCGGGCGGGCAGCACGTCAATACGACC
GATTCGGCTGTCCGAATCACGCATATCCCGACCGGCATCATGGTGGTGCAGGCGGAAAAATCGCAGCACCAGAACCGTGC
CCGCGCCATGCAGATCCTGCGCGCCCGCCTTTATGACATGGAGCGCCAGAAGGCTGAAAGCGAACGCTCGCAGGCGCGCC
GCAGCCAGGTTGGTTCGGGCGACCGGTCGGAGCGCATCCGTACCTATAATTTCCCGCAGGGGCGCGTCACCGACCATCGG
ATCAATCTCACGCTCTACAAGCTCGACCGCGTGATGGAAGGCGAACTGGACGAACTGGTCGATGCGCTGATTTCCGATCA
CCAGACCGCCCTTCTGGCCGAACTGGGCGAGCAGCCGTGA

Upstream 100 bases:

>100_bases
GAATCGTCGAACCGCTCTCATTATTCGTTATTGTCGCATTATCCGTATGCAAAACCGCTTCATGCTTTTGCCGGAAATGC
TCCAGTTTCTAGGACACGAC

Downstream 100 bases:

>100_bases
GCAGCGAAGAGGTGGAGCGCCTTGACCGGCTGATGGCAGAGGCCCGCGCGAAATTGCGTGCGGCGGGGGGCGAGACGCCC
GATCTCGATGCGCGTCTGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEASDLAAMRDDASTDAEMRALAV
EELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGTGGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGY
KEIIATVSGKGVFSKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGELDELVDALISDHQTALLAELGEQP

Sequences:

>Translated_359_residues
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEASDLAAMRDDASTDAEMRALAV
EELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGTGGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGY
KEIIATVSGKGVFSKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGELDELVDALISDHQTALLAELGEQP
>Mature_359_residues
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEASDLAAMRDDASTDAEMRALAV
EELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGTGGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGY
KEIIATVSGKGVFSKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGELDELVDALISDHQTALLAELGEQP

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=47.1947194719472, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI34577120, Length=361, Percent_Identity=37.6731301939058, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI166795305, Length=243, Percent_Identity=39.917695473251, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1787462, Length=335, Percent_Identity=58.8059701492537, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI2367172, Length=315, Percent_Identity=39.0476190476191, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=39.5017793594306, Blast_Score=187, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=44.3037974683544, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19921226, Length=326, Percent_Identity=42.3312883435583, Blast_Score=231, Evalue=8e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39909; Mature: 39909

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEAS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAMRDDASTDAEMRALAVEELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCC
GGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGYKEIIATVSGKGVFSKLKFES
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCCC
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMEGELDELVDALISDHQTALLAELGEQP
CCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEAS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAMRDDASTDAEMRALAVEELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCC
GGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGYKEIIATVSGKGVFSKLKFES
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCCC
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMEGELDELVDALISDHQTALLAELGEQP
CCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA