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The map label for this gene is ptsI [H]

Identifier: 225853309

GI number: 225853309

Start: 1824335

End: 1826605

Strand: Reverse

Name: ptsI [H]

Synonym: BMEA_A1924

Alternate gene names: 225853309

Gene position: 1826605-1824335 (Counterclockwise)

Preceding gene: 225853310

Following gene: 225853308

Centisome position: 85.93

GC content: 60.33

Gene sequence:

>2271_bases
ATGCGTGAGCTGACAACCGGTCCCCGTGTACTGCTCAAGCGGTTGCGTGAATTGATGGCGGAGCCGCTGGAACCACAGGC
GCGCCTTGACAGGATCGTCCGCGAAATTGCGCAGAACATGGTCGCGGAAGTCTGCTCCTTCTATATATTGCGCGCAGACG
GCGTTCTTGAACTTTACGCCACCGAAGGTCTTAATCCCCAAGCTGTCCACCTTGCCCAGCTTCGTCTGGGGCAGGGCCTT
GTGGGCACCATTGCGGCAAGCGCACGCCCGCTCAACCTGACGGATGCGCAGAGCCATCCGGCTTTCGCCTATCTGCCGGA
AACGGGCGAGGAAGCCTATAATTCCTTCCTCGGTGTGCCTGTGCTTCGCGCTGGTCGCACTTTAGGCGTTCTTGTCGTGC
AGAATCGCACCAAACGCGCCTATCGAGAAGACGAGGTTGAAGCACTTGAGACAACCGCCATGGTTCTCGCCGAAATCATC
GCAACCGGGGATGGTCTTCGTCTGGCGCGTCCAGGAATCGAACTGGATCTCGGGCGGCCGATGAGCGTAAGCGGCCACGG
TTTCAACGATGGTATCGGGCTTGGCCATGTGGTGCTGCATGAGCCGCGCATCGTCGTGACCAATCTTTTCAATGAGGACA
GTCAGGCCGAACTCAACCGGCTGGACGAGGCGCTCGGCTCCTTGCGTATTTCCATCGATGACATGTTGTCGCGCCGCGAT
GTGGCGGTGGAGGGCGAGCATCGCGAGGTGCTGGAAGCCTATCGCATGTTCGCGCATGATCGCGGCTGGGTGCGTCGTCT
GGAAGAAGCGGTTCATAACGGCCTTACGGCAGAAGCTGCCGTGGAAAAGGTGCAGAGCGACACGCGCGCGCGCATGGTGC
ATCTGACCGACCCCTATATGCGCGAGCGCCTTTCGGATTTCGACGATCTCGCCAATCGCCTCCTGCGCCAGCTTATGGGC
CGCGACGTCAAGACGATTGCCGAATCACTGGCCAAGGATGCGATCATCGTTGCCCGTTCCATGGGCGCGGCTGAACTTCT
GGACTATCCGCGCGAACGCCTGCGCGGTGTCGTGCTGGAAGACGGGGCGGCCACCAGCCATGTGGTGATCGTGGCGCGCG
CCATGGGTATTCCCGTCGTCGGGCAGGCCAAGGGTGTTGTTTCCATGGCGGAAAACAACGATGCGATCATCGTCGATGGC
GATGAGGGCATCGTGCATCTGCGCCCGCAGGCCGATGTGGAATCCGCCTATGCGGAAAAGGTCCGCTTCCGTGCACGGCG
GCAGGCGCATTACCGTGAATTGCGCGACAAGCCCGCCATCACGAAGGATGGCGTTGATATCGCGCTTTTGATGAATGCCG
GCTTGCTTGTCGATCTGCCGCAGCTTTCCGCTTCGGGCGCTGTCGGCATTGGCCTGTTTCGCACCGAGCTTCAATTCATG
GTGGCTTCCACCTTCCCGCGCTCGGAACAGCAGGAGCGGCTCTACCGTTCGGTGATCGATGCCGCAGGTGATAAGCCCGT
CACGTTCCGCACGCTGGATATTGGTGGCGACAAGGTTCTCCCCTATTTCCGCTCAACTGCGCATGAGGAAAACCCGGCGC
TCGGCTGGCGCGCCATTCGTTTGACGCTCGACCGTCCCGGCCTGTTGCGCACCCAGTTGCGCGCGCTATTGAAGGCGGCG
GGTGGCCGTGAACTGAAGCTCATGCTGCCCATGGTGACGGAAGTGAGCGAAGTGAAGGCCGCGCGCGAGATCATCGAACG
CGAGGTTCGCCATCTTTCCCGCTTTGCGCATTCGCTGCCGCTGTGTCTCAAGCTTGGCGCGATGGTGGAAGTGCCGGCGC
TATTGTGGCAGCTCGACGAATTGATGACGGCGGTGGATTTCGTTTCGGTGGGCTCCAACGACCTGTTCCAGTTCCTGATG
GCGGCGGATCGCGGCAATTCGCTGATTTCGGACCGTTTTGACCAGCTTTCACCATCGTTCCTGCGTGCGCTGCGTCATAT
CGTGAAAGCGGGGGCGCGGCATGAAAAGCCGGTGACGCTTTGCGGTGAAATGGCAGGCCGCCCCTTGACGGCAATGACGC
TGATCGGGCTTGGCTTCCGTTCGATTTCCATGTCGGCGGCGGCGATCGGCCCGGTCAAGGCCATGCTCGGCGCACTTGAT
GCGGGCAAGCTCAATGCGCTTCTTCTGGAAGAGCTCAACAAGCCGAACAATGCGCATTCCTTGCGCGAATTGTTGCTTCG
ATTTGCCGAAGACAACGAAATTCCGTTATAG

Upstream 100 bases:

>100_bases
GTCGAAAATGCTCACAAAGGGAATAGCGTAACGCTTTGCTGACGGGTGACAGGAATAGGCTTGGCGAAGCGGTTGCGCAA
AGAGAGGAGCCTCCGAGGAA

Downstream 100 bases:

>100_bases
CGAAGCCAATTGCTCAAATTGAACGGGTGCGGCGCGGCGGGCGCACGCATCTGGAACATGTTTGCACGCCGCAGCCGGAG
CGGTTGCGTTTTAACAGAAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 756; Mature: 756

Protein sequence:

>756_residues
MRELTTGPRVLLKRLRELMAEPLEPQARLDRIVREIAQNMVAEVCSFYILRADGVLELYATEGLNPQAVHLAQLRLGQGL
VGTIAASARPLNLTDAQSHPAFAYLPETGEEAYNSFLGVPVLRAGRTLGVLVVQNRTKRAYREDEVEALETTAMVLAEII
ATGDGLRLARPGIELDLGRPMSVSGHGFNDGIGLGHVVLHEPRIVVTNLFNEDSQAELNRLDEALGSLRISIDDMLSRRD
VAVEGEHREVLEAYRMFAHDRGWVRRLEEAVHNGLTAEAAVEKVQSDTRARMVHLTDPYMRERLSDFDDLANRLLRQLMG
RDVKTIAESLAKDAIIVARSMGAAELLDYPRERLRGVVLEDGAATSHVVIVARAMGIPVVGQAKGVVSMAENNDAIIVDG
DEGIVHLRPQADVESAYAEKVRFRARRQAHYRELRDKPAITKDGVDIALLMNAGLLVDLPQLSASGAVGIGLFRTELQFM
VASTFPRSEQQERLYRSVIDAAGDKPVTFRTLDIGGDKVLPYFRSTAHEENPALGWRAIRLTLDRPGLLRTQLRALLKAA
GGRELKLMLPMVTEVSEVKAAREIIEREVRHLSRFAHSLPLCLKLGAMVEVPALLWQLDELMTAVDFVSVGSNDLFQFLM
AADRGNSLISDRFDQLSPSFLRALRHIVKAGARHEKPVTLCGEMAGRPLTAMTLIGLGFRSISMSAAAIGPVKAMLGALD
AGKLNALLLEELNKPNNAHSLRELLLRFAEDNEIPL

Sequences:

>Translated_756_residues
MRELTTGPRVLLKRLRELMAEPLEPQARLDRIVREIAQNMVAEVCSFYILRADGVLELYATEGLNPQAVHLAQLRLGQGL
VGTIAASARPLNLTDAQSHPAFAYLPETGEEAYNSFLGVPVLRAGRTLGVLVVQNRTKRAYREDEVEALETTAMVLAEII
ATGDGLRLARPGIELDLGRPMSVSGHGFNDGIGLGHVVLHEPRIVVTNLFNEDSQAELNRLDEALGSLRISIDDMLSRRD
VAVEGEHREVLEAYRMFAHDRGWVRRLEEAVHNGLTAEAAVEKVQSDTRARMVHLTDPYMRERLSDFDDLANRLLRQLMG
RDVKTIAESLAKDAIIVARSMGAAELLDYPRERLRGVVLEDGAATSHVVIVARAMGIPVVGQAKGVVSMAENNDAIIVDG
DEGIVHLRPQADVESAYAEKVRFRARRQAHYRELRDKPAITKDGVDIALLMNAGLLVDLPQLSASGAVGIGLFRTELQFM
VASTFPRSEQQERLYRSVIDAAGDKPVTFRTLDIGGDKVLPYFRSTAHEENPALGWRAIRLTLDRPGLLRTQLRALLKAA
GGRELKLMLPMVTEVSEVKAAREIIEREVRHLSRFAHSLPLCLKLGAMVEVPALLWQLDELMTAVDFVSVGSNDLFQFLM
AADRGNSLISDRFDQLSPSFLRALRHIVKAGARHEKPVTLCGEMAGRPLTAMTLIGLGFRSISMSAAAIGPVKAMLGALD
AGKLNALLLEELNKPNNAHSLRELLLRFAEDNEIPL
>Mature_756_residues
MRELTTGPRVLLKRLRELMAEPLEPQARLDRIVREIAQNMVAEVCSFYILRADGVLELYATEGLNPQAVHLAQLRLGQGL
VGTIAASARPLNLTDAQSHPAFAYLPETGEEAYNSFLGVPVLRAGRTLGVLVVQNRTKRAYREDEVEALETTAMVLAEII
ATGDGLRLARPGIELDLGRPMSVSGHGFNDGIGLGHVVLHEPRIVVTNLFNEDSQAELNRLDEALGSLRISIDDMLSRRD
VAVEGEHREVLEAYRMFAHDRGWVRRLEEAVHNGLTAEAAVEKVQSDTRARMVHLTDPYMRERLSDFDDLANRLLRQLMG
RDVKTIAESLAKDAIIVARSMGAAELLDYPRERLRGVVLEDGAATSHVVIVARAMGIPVVGQAKGVVSMAENNDAIIVDG
DEGIVHLRPQADVESAYAEKVRFRARRQAHYRELRDKPAITKDGVDIALLMNAGLLVDLPQLSASGAVGIGLFRTELQFM
VASTFPRSEQQERLYRSVIDAAGDKPVTFRTLDIGGDKVLPYFRSTAHEENPALGWRAIRLTLDRPGLLRTQLRALLKAA
GGRELKLMLPMVTEVSEVKAAREIIEREVRHLSRFAHSLPLCLKLGAMVEVPALLWQLDELMTAVDFVSVGSNDLFQFLM
AADRGNSLISDRFDQLSPSFLRALRHIVKAGARHEKPVTLCGEMAGRPLTAMTLIGLGFRSISMSAAAIGPVKAMLGALD
AGKLNALLLEELNKPNNAHSLRELLLRFAEDNEIPL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=717, Percent_Identity=35.7043235704324, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788756, Length=483, Percent_Identity=34.7826086956522, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1788726, Length=550, Percent_Identity=33.8181818181818, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI48994992, Length=485, Percent_Identity=31.1340206185567, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83293; Mature: 83293

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00742 PEP_ENZYMES_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELTTGPRVLLKRLRELMAEPLEPQARLDRIVREIAQNMVAEVCSFYILRADGVLELYA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
TEGLNPQAVHLAQLRLGQGLVGTIAASARPLNLTDAQSHPAFAYLPETGEEAYNSFLGVP
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCH
VLRAGRTLGVLVVQNRTKRAYREDEVEALETTAMVLAEIIATGDGLRLARPGIELDLGRP
HHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCC
MSVSGHGFNDGIGLGHVVLHEPRIVVTNLFNEDSQAELNRLDEALGSLRISIDDMLSRRD
CCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHEEECHHHHHCCCC
VAVEGEHREVLEAYRMFAHDRGWVRRLEEAVHNGLTAEAAVEKVQSDTRARMVHLTDPYM
CEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHEEEECCCHHH
RERLSDFDDLANRLLRQLMGRDVKTIAESLAKDAIIVARSMGAAELLDYPRERLRGVVLE
HHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHCEEEEE
DGAATSHVVIVARAMGIPVVGQAKGVVSMAENNDAIIVDGDEGIVHLRPQADVESAYAEK
CCCCCCHHEEEEHHHCCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCCCCHHHHHHHH
VRFRARRQAHYRELRDKPAITKDGVDIALLMNAGLLVDLPQLSASGAVGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHH
VASTFPRSEQQERLYRSVIDAAGDKPVTFRTLDIGGDKVLPYFRSTAHEENPALGWRAIR
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEE
LTLDRPGLLRTQLRALLKAAGGRELKLMLPMVTEVSEVKAAREIIEREVRHLSRFAHSLP
EEECCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
LCLKLGAMVEVPALLWQLDELMTAVDFVSVGSNDLFQFLMAADRGNSLISDRFDQLSPSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHH
LRALRHIVKAGARHEKPVTLCGEMAGRPLTAMTLIGLGFRSISMSAAAIGPVKAMLGALD
HHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
AGKLNALLLEELNKPNNAHSLRELLLRFAEDNEIPL
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRELTTGPRVLLKRLRELMAEPLEPQARLDRIVREIAQNMVAEVCSFYILRADGVLELYA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
TEGLNPQAVHLAQLRLGQGLVGTIAASARPLNLTDAQSHPAFAYLPETGEEAYNSFLGVP
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCH
VLRAGRTLGVLVVQNRTKRAYREDEVEALETTAMVLAEIIATGDGLRLARPGIELDLGRP
HHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCC
MSVSGHGFNDGIGLGHVVLHEPRIVVTNLFNEDSQAELNRLDEALGSLRISIDDMLSRRD
CCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHEEECHHHHHCCCC
VAVEGEHREVLEAYRMFAHDRGWVRRLEEAVHNGLTAEAAVEKVQSDTRARMVHLTDPYM
CEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHEEEECCCHHH
RERLSDFDDLANRLLRQLMGRDVKTIAESLAKDAIIVARSMGAAELLDYPRERLRGVVLE
HHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHCEEEEE
DGAATSHVVIVARAMGIPVVGQAKGVVSMAENNDAIIVDGDEGIVHLRPQADVESAYAEK
CCCCCCHHEEEEHHHCCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCCCCHHHHHHHH
VRFRARRQAHYRELRDKPAITKDGVDIALLMNAGLLVDLPQLSASGAVGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHH
VASTFPRSEQQERLYRSVIDAAGDKPVTFRTLDIGGDKVLPYFRSTAHEENPALGWRAIR
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEE
LTLDRPGLLRTQLRALLKAAGGRELKLMLPMVTEVSEVKAAREIIEREVRHLSRFAHSLP
EEECCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
LCLKLGAMVEVPALLWQLDELMTAVDFVSVGSNDLFQFLMAADRGNSLISDRFDQLSPSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHH
LRALRHIVKAGARHEKPVTLCGEMAGRPLTAMTLIGLGFRSISMSAAAIGPVKAMLGALD
HHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
AGKLNALLLEELNKPNNAHSLRELLLRFAEDNEIPL
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]