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The map label for this gene is thrS [H]

Identifier: 225630645

GI number: 225630645

Start: 998610

End: 1000511

Strand: Reverse

Name: thrS [H]

Synonym: WRi_009280

Alternate gene names: 225630645

Gene position: 1000511-998610 (Counterclockwise)

Preceding gene: 225630646

Following gene: 225630644

Centisome position: 69.2

GC content: 38.59

Gene sequence:

>1902_bases
ATGATTAGGATTACTTTTTCAGCCGAACAAAAAGTAAAAGAATACAGTGGTAAAGTTACTGGCTTTGATATATTACAACC
GGATGTTTTGAAAGAAGCAATTGCCTTTAAGGTAAATGGTGAGTTGCATGATCTCTCACGTGAAATTGAAGCTGATGCAG
AGATAGAGGTGATACAACTGAGTGACGAAGCGGGTTTAGATATAATAAGGCATGATGCTGCTCACATAATGGCACAGGCA
GTGAAAGAGCTATTTCCTAATACTCAGATTACTATCGGCCCAACAATTCAGGACGGTTTTTACTATGATTTTGCTACAGG
TCGTACCTTCACTACGGACGATCTTACCGCAATAGAAAAGAAAATGAAAGAGATTGTAAAAAGTAACCACAGATTTGTTC
GAGAAGTTTGGACTCGCAAGCAGGCAATTGATTTCTTTAGTGATATAGGCGAAAAATATAAGGTTGATATTATCTCATCC
ATACCAGAGGGTGAAAACCTAACTGTTTATAGGCAAGGTGATTTTATTGACCTATGTCGTGGTCCGCACTCACCATCAAC
CGGCAGAGTTAAAGCGTTTAAACTTATGAAAGTAGCGGGTGCATACTGGCGAGGTGATGCAAAGGGCCCAATGTTGCAGC
GAATATATGGTACAGCATGGAGAAATAAGGATGAATTAAACGCTTACCTTGAGTGCCTCAAAGAAGCAGAAAAACGTGAT
CACCGCAAAATTGCTAAGGATATGGATTTATTTCACATTCAAGAGGAAGCTGTTGGGCAGGTTTTTTGGCATGAACAAGG
ATATATTCTATATAATGTTCTTGAGTCTTACATCAGAAAGAAGCTAATAAATAATGGCTATTTTGAGGTAAAAACTCCTA
TTTTAGTAAGCAAAGAGCTGTGGGAAAAATCTGGACATTGGGATAAGTTTCGTGAAAATATGTTTATTGTTGATGAATCT
GAAAGCAAAAAGCTAGCAATAAAACCCATGAATTGCCCCTGTCATGTGCAGATTTTTAATTCTCACACCAGAAGCTATCG
TGATTTACCGATACGTATGGCAGAATTTGGTACGTGTCATAGGAATGAAAGCTCAGGCTCATTGCACGGACTGATGCGAG
TGCGTGGTTTTACGCAAGACGATGCACACATTTTTTGTATGGAAGAACAAGTGAATTCTGAGACTATAAAGTTTTGTGAC
CTTTTAAAAGAAGTATATTCAGAGCTTGGGTTCAATGAAATTTCTGTGAAATTTTCAGACCGTCCAGATGTTAGGGCAGG
AGATGATGAAGTGTGGGATAGAGCTGAAAAAGCGCTGCTTGAAGCCGTTAAAGAAGCGGGCTTGAGTTATGAACTGAGCC
CTGGTGAAGGTGCATTTTATGGTCCAAAGCTAGAGTTTGTTTTGAAAGATGCAATAGGCAGAAGCTGGCAATGTGGAACA
TTGCAAGTTGATTTCATTTTACCAGAACGTCTGGGAGCTTTTTATATAGGAGCAGATGGGCACAAGCATCACCCTGTCAT
GTTACATAGGGCAATTCTTGGGACTTTTGAGCGTTTTATTGGAATTTTGATAGAAAATTACGCAGGAAAATTTCCAGTTT
GGCTTGCTCCAACGCAACTTGCTATTCTGACCATTACAAATGAGGCTGACGGTTACGCCACAAAAATCAGCAATGTCTTA
AAAGAACAAGGTGTAAGAGTCAAGACTGATTTGACCAATGAAAAAATTAGTTATAAGATACGTTTGCATAGTCTAAACAA
GGTTCCTATATTGTGGATTGTAGGCAAAAATGAAGTTACCAGTAAAACTGTGTCAGTGAGAAATTTAGGATCGGAAAGAC
AGGAGTCTTTTTCTTTGGAGAAGGCTATTGAATTGTTGTTAAAAAGTATTAATTTGAATTAA

Upstream 100 bases:

>100_bases
AAGCACACTATACAACATTTTCAATGAAATTACAAAAAAGCTGGATTCCAGTGTCAAGCACTGGGATGACAAAGAGAGGG
TTAGACAAAAGGGTCGATAA

Downstream 100 bases:

>100_bases
GGGAGTTAAAGTTTGCAAGTTAAAAAGAACAATAAAAACAGAATTAATGGATTCATCACAGCTAAGGAGGTACGCTTAGT
TGATCACAGTGGTGAAATGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MIRITFSAEQKVKEYSGKVTGFDILQPDVLKEAIAFKVNGELHDLSREIEADAEIEVIQLSDEAGLDIIRHDAAHIMAQA
VKELFPNTQITIGPTIQDGFYYDFATGRTFTTDDLTAIEKKMKEIVKSNHRFVREVWTRKQAIDFFSDIGEKYKVDIISS
IPEGENLTVYRQGDFIDLCRGPHSPSTGRVKAFKLMKVAGAYWRGDAKGPMLQRIYGTAWRNKDELNAYLECLKEAEKRD
HRKIAKDMDLFHIQEEAVGQVFWHEQGYILYNVLESYIRKKLINNGYFEVKTPILVSKELWEKSGHWDKFRENMFIVDES
ESKKLAIKPMNCPCHVQIFNSHTRSYRDLPIRMAEFGTCHRNESSGSLHGLMRVRGFTQDDAHIFCMEEQVNSETIKFCD
LLKEVYSELGFNEISVKFSDRPDVRAGDDEVWDRAEKALLEAVKEAGLSYELSPGEGAFYGPKLEFVLKDAIGRSWQCGT
LQVDFILPERLGAFYIGADGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQLAILTITNEADGYATKISNVL
KEQGVRVKTDLTNEKISYKIRLHSLNKVPILWIVGKNEVTSKTVSVRNLGSERQESFSLEKAIELLLKSINLN

Sequences:

>Translated_633_residues
MIRITFSAEQKVKEYSGKVTGFDILQPDVLKEAIAFKVNGELHDLSREIEADAEIEVIQLSDEAGLDIIRHDAAHIMAQA
VKELFPNTQITIGPTIQDGFYYDFATGRTFTTDDLTAIEKKMKEIVKSNHRFVREVWTRKQAIDFFSDIGEKYKVDIISS
IPEGENLTVYRQGDFIDLCRGPHSPSTGRVKAFKLMKVAGAYWRGDAKGPMLQRIYGTAWRNKDELNAYLECLKEAEKRD
HRKIAKDMDLFHIQEEAVGQVFWHEQGYILYNVLESYIRKKLINNGYFEVKTPILVSKELWEKSGHWDKFRENMFIVDES
ESKKLAIKPMNCPCHVQIFNSHTRSYRDLPIRMAEFGTCHRNESSGSLHGLMRVRGFTQDDAHIFCMEEQVNSETIKFCD
LLKEVYSELGFNEISVKFSDRPDVRAGDDEVWDRAEKALLEAVKEAGLSYELSPGEGAFYGPKLEFVLKDAIGRSWQCGT
LQVDFILPERLGAFYIGADGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQLAILTITNEADGYATKISNVL
KEQGVRVKTDLTNEKISYKIRLHSLNKVPILWIVGKNEVTSKTVSVRNLGSERQESFSLEKAIELLLKSINLN
>Mature_633_residues
MIRITFSAEQKVKEYSGKVTGFDILQPDVLKEAIAFKVNGELHDLSREIEADAEIEVIQLSDEAGLDIIRHDAAHIMAQA
VKELFPNTQITIGPTIQDGFYYDFATGRTFTTDDLTAIEKKMKEIVKSNHRFVREVWTRKQAIDFFSDIGEKYKVDIISS
IPEGENLTVYRQGDFIDLCRGPHSPSTGRVKAFKLMKVAGAYWRGDAKGPMLQRIYGTAWRNKDELNAYLECLKEAEKRD
HRKIAKDMDLFHIQEEAVGQVFWHEQGYILYNVLESYIRKKLINNGYFEVKTPILVSKELWEKSGHWDKFRENMFIVDES
ESKKLAIKPMNCPCHVQIFNSHTRSYRDLPIRMAEFGTCHRNESSGSLHGLMRVRGFTQDDAHIFCMEEQVNSETIKFCD
LLKEVYSELGFNEISVKFSDRPDVRAGDDEVWDRAEKALLEAVKEAGLSYELSPGEGAFYGPKLEFVLKDAIGRSWQCGT
LQVDFILPERLGAFYIGADGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQLAILTITNEADGYATKISNVL
KEQGVRVKTDLTNEKISYKIRLHSLNKVPILWIVGKNEVTSKTVSVRNLGSERQESFSLEKAIELLLKSINLN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=631, Percent_Identity=38.8272583201268, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI38570111, Length=613, Percent_Identity=38.3360522022839, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI20070344, Length=652, Percent_Identity=35.7361963190184, Blast_Score=426, Evalue=1e-119,
Organism=Escherichia coli, GI1788013, Length=622, Percent_Identity=50.4823151125402, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=167, Percent_Identity=25.748502994012, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71984184, Length=627, Percent_Identity=37.0015948963317, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71984192, Length=627, Percent_Identity=37.0015948963317, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322112, Length=613, Percent_Identity=39.4779771615008, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322654, Length=407, Percent_Identity=35.6265356265356, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24583842, Length=632, Percent_Identity=38.9240506329114, Blast_Score=470, Evalue=1e-132,
Organism=Drosophila melanogaster, GI28574141, Length=632, Percent_Identity=38.9240506329114, Blast_Score=470, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24583839, Length=632, Percent_Identity=38.9240506329114, Blast_Score=470, Evalue=1e-132,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72339; Mature: 72339

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRITFSAEQKVKEYSGKVTGFDILQPDVLKEAIAFKVNGELHDLSREIEADAEIEVIQL
CEEEEECHHHHHHHHCCCCCEEECCCHHHHHHHHEEEECCCHHHHHHHHCCCCCEEEEEE
SDEAGLDIIRHDAAHIMAQAVKELFPNTQITIGPTIQDGFYYDFATGRTFTTDDLTAIEK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEECCCCEECCHHHHHHHH
KMKEIVKSNHRFVREVWTRKQAIDFFSDIGEKYKVDIISSIPEGENLTVYRQGDFIDLCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCEEEECC
GPHSPSTGRVKAFKLMKVAGAYWRGDAKGPMLQRIYGTAWRNKDELNAYLECLKEAEKRD
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRKIAKDMDLFHIQEEAVGQVFWHEQGYILYNVLESYIRKKLINNGYFEVKTPILVSKEL
HHHHHHHHHHHEECHHHHHHHEECCCCEEHHHHHHHHHHHHHHCCCCEEEECCEEECHHH
WEKSGHWDKFRENMFIVDESESKKLAIKPMNCPCHVQIFNSHTRSYRDLPIRMAEFGTCH
HHCCCCHHHHHCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCCHHCCCEEHHHCCCCC
RNESSGSLHGLMRVRGFTQDDAHIFCMEEQVNSETIKFCDLLKEVYSELGFNEISVKFSD
CCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
RPDVRAGDDEVWDRAEKALLEAVKEAGLSYELSPGEGAFYGPKLEFVLKDAIGRSWQCGT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCHHHHHHHHHCCCCCCCCE
LQVDFILPERLGAFYIGADGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQL
EEEEEEEHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEE
AILTITNEADGYATKISNVLKEQGVRVKTDLTNEKISYKIRLHSLNKVPILWIVGKNEVT
EEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCCC
SKTVSVRNLGSERQESFSLEKAIELLLKSINLN
HHEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRITFSAEQKVKEYSGKVTGFDILQPDVLKEAIAFKVNGELHDLSREIEADAEIEVIQL
CEEEEECHHHHHHHHCCCCCEEECCCHHHHHHHHEEEECCCHHHHHHHHCCCCCEEEEEE
SDEAGLDIIRHDAAHIMAQAVKELFPNTQITIGPTIQDGFYYDFATGRTFTTDDLTAIEK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEECCCCEECCHHHHHHHH
KMKEIVKSNHRFVREVWTRKQAIDFFSDIGEKYKVDIISSIPEGENLTVYRQGDFIDLCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCEEEECC
GPHSPSTGRVKAFKLMKVAGAYWRGDAKGPMLQRIYGTAWRNKDELNAYLECLKEAEKRD
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRKIAKDMDLFHIQEEAVGQVFWHEQGYILYNVLESYIRKKLINNGYFEVKTPILVSKEL
HHHHHHHHHHHEECHHHHHHHEECCCCEEHHHHHHHHHHHHHHCCCCEEEECCEEECHHH
WEKSGHWDKFRENMFIVDESESKKLAIKPMNCPCHVQIFNSHTRSYRDLPIRMAEFGTCH
HHCCCCHHHHHCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCCHHCCCEEHHHCCCCC
RNESSGSLHGLMRVRGFTQDDAHIFCMEEQVNSETIKFCDLLKEVYSELGFNEISVKFSD
CCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
RPDVRAGDDEVWDRAEKALLEAVKEAGLSYELSPGEGAFYGPKLEFVLKDAIGRSWQCGT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCHHHHHHHHHCCCCCCCCE
LQVDFILPERLGAFYIGADGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQL
EEEEEEEHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEE
AILTITNEADGYATKISNVLKEQGVRVKTDLTNEKISYKIRLHSLNKVPILWIVGKNEVT
EEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCCC
SKTVSVRNLGSERQESFSLEKAIELLLKSINLN
HHEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA