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The map label for this gene is nuoI [H]

Identifier: 225630646

GI number: 225630646

Start: 1000815

End: 1001324

Strand: Reverse

Name: nuoI [H]

Synonym: WRi_009290

Alternate gene names: 225630646

Gene position: 1001324-1000815 (Counterclockwise)

Preceding gene: 225630649

Following gene: 225630645

Centisome position: 69.25

GC content: 38.43

Gene sequence:

>510_bases
ATGACTGACACAAGTTTTAAATATTTTATGCTCAAGAAATTAGCTTGGTACTGGTCTTTTGTAGAGTTAATTAAAGGGTT
TGTTATTACATTAAAATATATGTTTAAGCCAAAGGTTACTTTGAGGTATCCTATGGAGAAGGGCCCTTTAAGTCCAAGGT
TTCGTGGTGAGCATGCGTTGCGTAGGTATCCAAACGGTGAAGAACGATGCATAGCTTGTAAATTATGTGAAGTTATCTGC
CCTGCTCAAGCAATAGTTATTGAAGCAGAGGAAAGAGAAGACGGTAGTCGCCGCACCACGCGCTATGATATTGATATGAC
AAAATGCATATACTGCGGACTTTGCCAAGAGGCATGTCCAGTTGATGCAATTGTTGAAGGTCCTAACTTTGAATTTGCTA
CTGAAACAAGAGAGGAGCTAATGTACAATAAAGAAAAGTTATTGCGTAATGGTGAAGTTTGGGAAGACGCAATTGCACTC
AGGTTAAAGAAGAATAGGCCTTATTATTAA

Upstream 100 bases:

>100_bases
TATATAAACTACTTAGCAATGTTATACTTAAGGAACCAAAATACCTAAAAAGTCACCCAGGTGAAAATATTTATTTTGAA
AACTGATAATATGATTTATA

Downstream 100 bases:

>100_bases
TGTTCAGAGCTTATAACCCTGTTATCCATTATGATTTAGGGGCAGTGCCCACTAGGGGTGGTGTCATCCCAGTAGCTGAT
ACACAACTGTACGAACATTT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MTDTSFKYFMLKKLAWYWSFVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEERCIACKLCEVIC
PAQAIVIEAEEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEDAIAL
RLKKNRPYY

Sequences:

>Translated_169_residues
MTDTSFKYFMLKKLAWYWSFVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEERCIACKLCEVIC
PAQAIVIEAEEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEDAIAL
RLKKNRPYY
>Mature_168_residues
TDTSFKYFMLKKLAWYWSFVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEERCIACKLCEVICP
AQAIVIEAEEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEDAIALR
LKKNRPYY

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=149, Percent_Identity=74.496644295302, Blast_Score=239, Evalue=8e-64,
Organism=Escherichia coli, GI1788617, Length=140, Percent_Identity=40.7142857142857, Blast_Score=108, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555194, Length=144, Percent_Identity=75.6944444444444, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17864306, Length=149, Percent_Identity=77.1812080536913, Blast_Score=249, Evalue=8e-67,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19966; Mature: 19835

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTSFKYFMLKKLAWYWSFVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHH
RRYPNGEERCIACKLCEVICPAQAIVIEAEEREDGSRRTTRYDIDMTKCIYCGLCQEACP
HCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHCC
VDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEDAIALRLKKNRPYY
CCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
TDTSFKYFMLKKLAWYWSFVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHH
RRYPNGEERCIACKLCEVICPAQAIVIEAEEREDGSRRTTRYDIDMTKCIYCGLCQEACP
HCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHCC
VDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEDAIALRLKKNRPYY
CCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA