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The map label for this gene is nuoM [H]

Identifier: 225630635

GI number: 225630635

Start: 987924

End: 989366

Strand: Direct

Name: nuoM [H]

Synonym: WRi_009140

Alternate gene names: 225630635

Gene position: 987924-989366 (Clockwise)

Preceding gene: 225630634

Following gene: 225630636

Centisome position: 68.33

GC content: 35.07

Gene sequence:

>1443_bases
GTGTTGTTACTTAGTATATTCTTGCTTCCACTGATAGGAGCGTTGATTTTATCCTTAATCAGGATTAATCATCAATCTAT
ACACTTAAGATTCCTTGCTCTATTTTTTGCTGTACTTCCATTTTTGCTTAGCATTGTAGCTTGTATAGAATTTGATTATA
ACGATGCGGACTTTCAGTTTGTCAGCTACCCAATTAGAAATGTTGGAATAGGGATAGATGGTATATCGTTGCTTTTCCTT
CTGCTTACAACTTTCTTGTTTGTAATTTGTATACTCTACAACTGCAAAATGAGTTATACAACCCTTAGGCCATATATGGC
ATTATTTCTACTGCTTGAGAGTTTTGTAGTCGGTTTTTTCGTTTCACTGAATGCTATAAGCTTTTATGTGTTTTTTGAAG
CTGTTTTAATACCAATGTTCTTTATTATTGGCATTTGGGGAGGAAAACACAGGGTATATGCAACGTTTAAGTTGTTTCTT
TATACATTAACTGGCTCATTATTATTTTTACTTGGATTGGTGTACATCTATAGCACTTTTGGGACGTTTAATATACAAAA
ATTAGCTACATTAGTGCCAAACCTTGATCTTGAAGTGCAGTCATTGCTGTGGATTGCATTTTTTATTTCTTTTGCAATAA
AAGTACCGATGTTTCCATTTCACACTTGGCTTCCTGATGCGCATGTGCAATCACCAACTTCTGGATCTGTGATTTTAGCT
GGCTTGCTTATTAAAATGGGGGGATATGGATTTTTAAGGTTTTCTATTCCAATGCTTCCTCAGGCAAGTTTATATTTTTC
AAATTTCGTTGTTGTGCTGAGCATTATTGCGGTGATATATGCTTCTCTAGTTGCGTTTGCTCAAGATGATATAAAGAAGT
TAATAGCTTATTCTTCAATAGCACATATGGGGATCGTTACTGCTGGCCTCTTTTCATTTTGTGAGGAAGGAGTACTGGGT
AGCATATTTCAAATGATCAGTCATGGCCTTATCTCTGCTGCTTTATTTTTATGTGTTGGAATGCTATATACTCGAACTGG
AACTTTGGAGATTGCAAAATATTTTGGCATAGTAAACACAATGCCAAAATTTGGTTTTATGTTCATTTTATTTTCAATAG
CTTCAATAGGGCTACCTGGAACATCTGGGTTCGCAGGTGAGTTTTTGGCTATGGTTGGAATGTTTAAGAGCCTAGGGTTT
TTTACAGGATTTATCGCACTTGGCACTATTTTAAGCGCAGTTTATATGCTGAATTTATGTAAGCAAATAATATGGGGGGT
TAGCTATTCTAAATTATTAAATAACCGCTTGGATAGCATAGAATTTTCTGTCTTAATTCTGCTTGCAGTGTTTGTTATTT
TGCTTGGATTCTACCCAACCCTTGCACTGAATTATTTGAAGCCATGTATGGCAAATTTGTTAGTCAAATATAATGCGCTA
TGA

Upstream 100 bases:

>100_bases
TTCTATAAAGTTACAAACTGGTTATATATTTGATTATGCATTTATTATGTTTGTTACTTTAATAATCGGTGCTTTATATA
TTATTGGAATTAAATAGAAG

Downstream 100 bases:

>100_bases
ATTATATACAGATATTGCCGGAAACGTTCTCTATTATCTCCTCGTTAGTGTTGCTGCTGCTTGGGATTATATTTAACCGC
CGCACTATCAATTTACTAGC

Product: NADH dehydrogenase I, M subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLLLSIFLLPLIGALILSLIRINHQSIHLRFLALFFAVLPFLLSIVACIEFDYNDADFQFVSYPIRNVGIGIDGISLLFL
LLTTFLFVICILYNCKMSYTTLRPYMALFLLLESFVVGFFVSLNAISFYVFFEAVLIPMFFIIGIWGGKHRVYATFKLFL
YTLTGSLLFLLGLVYIYSTFGTFNIQKLATLVPNLDLEVQSLLWIAFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSIAHMGIVTAGLFSFCEEGVLG
SIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNTMPKFGFMFILFSIASIGLPGTSGFAGEFLAMVGMFKSLGF
FTGFIALGTILSAVYMLNLCKQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL

Sequences:

>Translated_480_residues
MLLLSIFLLPLIGALILSLIRINHQSIHLRFLALFFAVLPFLLSIVACIEFDYNDADFQFVSYPIRNVGIGIDGISLLFL
LLTTFLFVICILYNCKMSYTTLRPYMALFLLLESFVVGFFVSLNAISFYVFFEAVLIPMFFIIGIWGGKHRVYATFKLFL
YTLTGSLLFLLGLVYIYSTFGTFNIQKLATLVPNLDLEVQSLLWIAFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSIAHMGIVTAGLFSFCEEGVLG
SIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNTMPKFGFMFILFSIASIGLPGTSGFAGEFLAMVGMFKSLGF
FTGFIALGTILSAVYMLNLCKQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL
>Mature_480_residues
MLLLSIFLLPLIGALILSLIRINHQSIHLRFLALFFAVLPFLLSIVACIEFDYNDADFQFVSYPIRNVGIGIDGISLLFL
LLTTFLFVICILYNCKMSYTTLRPYMALFLLLESFVVGFFVSLNAISFYVFFEAVLIPMFFIIGIWGGKHRVYATFKLFL
YTLTGSLLFLLGLVYIYSTFGTFNIQKLATLVPNLDLEVQSLLWIAFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSIAHMGIVTAGLFSFCEEGVLG
SIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNTMPKFGFMFILFSIASIGLPGTSGFAGEFLAMVGMFKSLGF
FTGFIALGTILSAVYMLNLCKQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=337, Percent_Identity=33.2344213649852, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI251831117, Length=266, Percent_Identity=25.9398496240602, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1788613, Length=425, Percent_Identity=36.4705882352941, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788827, Length=360, Percent_Identity=25.8333333333333, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788831, Length=278, Percent_Identity=26.6187050359712, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788829, Length=408, Percent_Identity=25.9803921568627, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI2367154, Length=243, Percent_Identity=25.9259259259259, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693160, Length=376, Percent_Identity=25, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788614, Length=366, Percent_Identity=25.1366120218579, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53414; Mature: 53414

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLSIFLLPLIGALILSLIRINHQSIHLRFLALFFAVLPFLLSIVACIEFDYNDADFQF
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VSYPIRNVGIGIDGISLLFLLLTTFLFVICILYNCKMSYTTLRPYMALFLLLESFVVGFF
HCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VSLNAISFYVFFEAVLIPMFFIIGIWGGKHRVYATFKLFLYTLTGSLLFLLGLVYIYSTF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTFNIQKLATLVPNLDLEVQSLLWIAFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHH
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSI
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHMGIVTAGLFSFCEEGVLGSIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MPKFGFMFILFSIASIGLPGTSGFAGEFLAMVGMFKSLGFFTGFIALGTILSAVYMLNLC
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA