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The map label for this gene is nuoN [H]

Identifier: 225630636

GI number: 225630636

Start: 989363

End: 990727

Strand: Direct

Name: nuoN [H]

Synonym: WRi_009150

Alternate gene names: 225630636

Gene position: 989363-990727 (Clockwise)

Preceding gene: 225630635

Following gene: 225630637

Centisome position: 68.43

GC content: 34.58

Gene sequence:

>1365_bases
ATGAATTATATACAGATATTGCCGGAAACGTTCTCTATTATCTCCTCGTTAGTGTTGCTGCTGCTTGGGATTATATTTAA
CCGCCGCACTATCAATTTACTAGCACTTGGCTGCACAGTGGTAACTTTGATTATTTTAATTATTTCGGCAGAAAACAATG
AAATTTTTCTCTTTAATTCGTTGTTAAAACTCAATTTATACATCAGGTCAGCCCAAGGGTTAATTCTCAGCACAGGAATT
TTAATACTTTTGATGCTGAATTTATCAAAATATGACTATAAATATGAATTTTCAATACTGATTCTTTTTGCGCTATTTGG
CATGATAACTTTAGTTTCAGCAAATAGCTTAATTTCTTTCTATTTAGCTTTTGAGTTGATGAGTATATCTTTGTATGTTC
TTGCGAGCTTTAATAAAGATTCAGCTTATTCATGCGAAGCAGGAGTGAAATATTTTACACTCAGTGCGTTATCTTCCTGC
ATTATGCTATATGGAATGTCGCTGCTTTATGGATATACAGGACAAGTCAATTTCTCTGAGCTCAGTTCATTCTTGCAAAA
CCACCAGATAACTTATGGAATAGTTTTTGGGTTAGTCTTTGTCCTAATTGGTTTGTGTTTCAAGCTTGCTATTGTTCCTT
TTCATATGTGGGCTCCAGATGTCTATCAAGGTGCACCTACCATAGTAACTGCGTTTTTTTCTACAGCTCCAAAAGCTGCA
CTTGTAACATTTTTAATTCGATTGATGAATGAAGAGTTAGTAAATGTAAAAAGTTATGTTCAGCCTATTTTCTTATACGT
TTCAGCATTGTCTGTGCTTATCTCAGCTTTTGGGGCCTTGCGTCAGCAAAACTTAAAAAGGCTGCTTGCTTACAGTTCAA
TTGGTCACATTGGTTTTATATTTGCTTCACTTTCTATTTTTACACAAGCAGGAACAGATAGTGCCTTAATGTATCTGGTG
ATATATATCATCACAAGCATAGGGCTATTCTCATATCTTGTACAAATTGACGATGACGATTGTGATATTGCAAATTTATC
TGGTATAGGGAAGAAACGCCCAATTGTAGCATTTCATCTTTCTGTGCTGTTACTCTCGATGTCGGGAATACCTCCACTTG
CAGGTTTTATCGCTAAATTTTTTATATTCAAAAGTTTAATAAATTCTGGCTTTATCAGCCTGTCCTTGATCCTTGTAATA
GCGAGTGTGATATCATGCTACTATTATTTAAATATCATGAAAGTTATGTATTTTGATAAAGCTAGTGGTAATAAGGTTGC
TTATTCTAAGGGTCTATTCATTATTACTTCAGTGGCTTCACTGATCAACCTCGTTCTTTTCTTGTACGTTTTACTCACTC
ATTGA

Upstream 100 bases:

>100_bases
TGCTTGCAGTGTTTGTTATTTTGCTTGGATTCTACCCAACCCTTGCACTGAATTATTTGAAGCCATGTATGGCAAATTTG
TTAGTCAAATATAATGCGCT

Downstream 100 bases:

>100_bases
ACTAAAGGACTGACAAGGTGATCCCTGAGGGTTTTCATATTCATCATTACAAAGAAGTTTCAAGCACTAATAAAGAAGCG
TTGGATTTAATTGATAAAGG

Product: NADH dehydrogenase I, N subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNYIQILPETFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILIISAENNEIFLFNSLLKLNLYIRSAQGLILSTGI
LILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISFYLAFELMSISLYVLASFNKDSAYSCEAGVKYFTLSALSSC
IMLYGMSLLYGYTGQVNFSELSSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFIFASLSIFTQAGTDSALMYLV
IYIITSIGLFSYLVQIDDDDCDIANLSGIGKKRPIVAFHLSVLLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVI
ASVISCYYYLNIMKVMYFDKASGNKVAYSKGLFIITSVASLINLVLFLYVLLTH

Sequences:

>Translated_454_residues
MNYIQILPETFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILIISAENNEIFLFNSLLKLNLYIRSAQGLILSTGI
LILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISFYLAFELMSISLYVLASFNKDSAYSCEAGVKYFTLSALSSC
IMLYGMSLLYGYTGQVNFSELSSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFIFASLSIFTQAGTDSALMYLV
IYIITSIGLFSYLVQIDDDDCDIANLSGIGKKRPIVAFHLSVLLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVI
ASVISCYYYLNIMKVMYFDKASGNKVAYSKGLFIITSVASLINLVLFLYVLLTH
>Mature_454_residues
MNYIQILPETFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILIISAENNEIFLFNSLLKLNLYIRSAQGLILSTGI
LILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISFYLAFELMSISLYVLASFNKDSAYSCEAGVKYFTLSALSSC
IMLYGMSLLYGYTGQVNFSELSSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFIFASLSIFTQAGTDSALMYLV
IYIITSIGLFSYLVQIDDDDCDIANLSGIGKKRPIVAFHLSVLLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVI
ASVISCYYYLNIMKVMYFDKASGNKVAYSKGLFIITSVASLINLVLFLYVLLTH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=280, Percent_Identity=25, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693160, Length=373, Percent_Identity=33.7801608579088, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI1788613, Length=370, Percent_Identity=24.8648648648649, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788614, Length=325, Percent_Identity=24.9230769230769, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788831, Length=295, Percent_Identity=27.4576271186441, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788827, Length=296, Percent_Identity=23.3108108108108, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788829, Length=287, Percent_Identity=25.4355400696864, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI2367154, Length=302, Percent_Identity=26.4900662251656, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50301; Mature: 50301

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIQILPETFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILIISAENNEIFLFNS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEEH
LLKLNLYIRSAQGLILSTGILILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISF
HHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAFELMSISLYVLASFNKDSAYSCEAGVKYFTLSALSSCIMLYGMSLLYGYTGQVNFSE
HHHHHHHHHHHHHEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LSSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCHHH
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FASLSIFTQAGTDSALMYLVIYIITSIGLFSYLVQIDDDDCDIANLSGIGKKRPIVAFHL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHH
SVLLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVIASVISCYYYLNIMKVMYFDK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
ASGNKVAYSKGLFIITSVASLINLVLFLYVLLTH
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYIQILPETFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILIISAENNEIFLFNS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEEH
LLKLNLYIRSAQGLILSTGILILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISF
HHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAFELMSISLYVLASFNKDSAYSCEAGVKYFTLSALSSCIMLYGMSLLYGYTGQVNFSE
HHHHHHHHHHHHHEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LSSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCHHH
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FASLSIFTQAGTDSALMYLVIYIITSIGLFSYLVQIDDDDCDIANLSGIGKKRPIVAFHL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHH
SVLLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVIASVISCYYYLNIMKVMYFDK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
ASGNKVAYSKGLFIITSVASLINLVLFLYVLLTH
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA