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The map label for this gene is nuoL [H]

Identifier: 225630634

GI number: 225630634

Start: 986064

End: 987920

Strand: Direct

Name: nuoL [H]

Synonym: WRi_009130

Alternate gene names: 225630634

Gene position: 986064-987920 (Clockwise)

Preceding gene: 225630633

Following gene: 225630635

Centisome position: 68.2

GC content: 34.95

Gene sequence:

>1857_bases
ATGACGTATATACTGAAATTAATAGTATTTTTGCCACTTTTTGGTTCGCTATTTGCAGCACTTTTTAGAAGAGATGTTTT
TAGTCAGTTAGTTACAACGGCAGGGATTGGAATATCTGCAGTTTTATCTTGGTATGTTTTTCTCACCTTCTCTGAAAATT
ATCACTTGAGTTTATTTCCTTTGTTTTCATTGAGCATCTTAAAAGTAAATTGGGCAATTAGTGTGGATGCTCTCTCATCC
TTAATGCTGATTGTTATTACCACCGTTTCACTGGTAGTACATCTCTACTCTATCGGTTATATGGAGCATGATAAGGGGAA
GTCGAGGTTTTTTTCTTACCTATCGCTATTTACGTTTTGCATGATTGTGCTGGTTGTAAGCGATAATTTCGTGCAGCTTT
TTTTTGGCTGGGAAGGAGTTGGCCTGTGTTCTTATTTACTCATAGGATTTTGGTTCCAAAAATATTCTGCAAATAATGCA
GCAATTAAAGCATTTGTTGTGAATAGGGTAGGAGACTTTGCACTATTGATTGGGATCTTTCTTATTTATTATACATTTCA
CTCTTTGAAATTTACTGAAGTTTTTGACACAGCTGATCTTCTTGGCACGCAGAACATTAGAGCATTCTGCTGTGAATTCA
AAGTAACTCATATAATATGCATATTACTTTTTATTGGCTGTATGGGTAAATCTGCACAGCTTGGTTTACATGTTTGGTTG
CCAGATGCAATGGAAGGGCCAACTCCTGTTTCTGCACTTATTCACGCAGCAACGATGGTAACAGCAGGTATATTTTTAGT
AGCAAAGTGTTCTCCATTGTTTGAGTTATCAAATGTGGCACGAGAATTAATAGTTATCGTTGGAGCACTCACTGCTTTTT
TTGCAGCCACCGTTGCAATTACTCAGAACGATATAAAGAAAATAATTGCTTATTCAACCTGCAGTCAACTTGGTTATATG
TTCATGGCATGTGGGCTTTCTGCTTACAATGTTGCTATTTTTCATTTAATGACCCACGCTTTTTTTAAAGCTCTACTATT
TCTTGGTGCTGGCAATGTAATTCATGCAATGCATCATGAGCAAAACATTCAGAAAATGGGAAATTGCTGGAAGAAAATCC
CTTGCACTTACACTCTCATGTGGATTGGGTCTCTTGCGCTTTCTGGAATATTTCCATTTGCAGGTTTTTACTCAAAGGAT
TTGATAATTGAACATGCTTATAGCACGGATAGCTTTGCTTTTGTAATAAGCTTAGTTGTTGCATTCTTCACAGCGTTTTA
CTCTTGGAGGTTATTGCTTCTTGTGTTCCACAGCCAAAAACAAAGTAAAATTAATATACATGAGGCACCAAAGATTATGC
TTATACCATTACTGATACTTGCTTTTGGTTCGGTATTTTCTGGAGTGTGGGGAGCAAATATTTTGAACATAACTAGTAAT
GCGTTTTGGAAATCAAGTTTAGTAGTTATTGATGAGCATGGAGTTCATAATTTCTTTATAAAACTACTGCCAACCTTAGC
AAGCCTAAGTGGAATAGCACTTGCGTACCTAATTTACCAATATCAAGTAATTAGGCAAATTAAGAGTAAATTTTTACTTA
AGTTTTTGCAGAATAAATGGTACTTTGATGAGGTGTATGAGTTTGTTATCATTGCACCGATAAGGTTTATATCTAGGCTT
CTATGGAAGTTTGATGTTAAGGCTATTGATTCATTTGGGCCAAATGGTATTGTAAGGTTGGTTAATGAATGTTCAAAAAG
TTCTATAAAGTTACAAACTGGTTATATATTTGATTATGCATTTATTATGTTTGTTACTTTAATAATCGGTGCTTTATATA
TTATTGGAATTAAATAG

Upstream 100 bases:

>100_bases
GCAGCAGAGTCAGGAGTTGGGCTTGCGATATTGGTTGTATATTATAGAAGCCGTGGCAATATAGACGTTGAACAAGCAAA
TTTAATGAAAGAATGAAAGT

Downstream 100 bases:

>100_bases
AAGGTGTTGTTACTTAGTATATTCTTGCTTCCACTGATAGGAGCGTTGATTTTATCCTTAATCAGGATTAATCATCAATC
TATACACTTAAGATTCCTTG

Product: NADH dehydrogenase I, L subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MTYILKLIVFLPLFGSLFAALFRRDVFSQLVTTAGIGISAVLSWYVFLTFSENYHLSLFPLFSLSILKVNWAISVDALSS
LMLIVITTVSLVVHLYSIGYMEHDKGKSRFFSYLSLFTFCMIVLVVSDNFVQLFFGWEGVGLCSYLLIGFWFQKYSANNA
AIKAFVVNRVGDFALLIGIFLIYYTFHSLKFTEVFDTADLLGTQNIRAFCCEFKVTHIICILLFIGCMGKSAQLGLHVWL
PDAMEGPTPVSALIHAATMVTAGIFLVAKCSPLFELSNVARELIVIVGALTAFFAATVAITQNDIKKIIAYSTCSQLGYM
FMACGLSAYNVAIFHLMTHAFFKALLFLGAGNVIHAMHHEQNIQKMGNCWKKIPCTYTLMWIGSLALSGIFPFAGFYSKD
LIIEHAYSTDSFAFVISLVVAFFTAFYSWRLLLLVFHSQKQSKINIHEAPKIMLIPLLILAFGSVFSGVWGANILNITSN
AFWKSSLVVIDEHGVHNFFIKLLPTLASLSGIALAYLIYQYQVIRQIKSKFLLKFLQNKWYFDEVYEFVIIAPIRFISRL
LWKFDVKAIDSFGPNGIVRLVNECSKSSIKLQTGYIFDYAFIMFVTLIIGALYIIGIK

Sequences:

>Translated_618_residues
MTYILKLIVFLPLFGSLFAALFRRDVFSQLVTTAGIGISAVLSWYVFLTFSENYHLSLFPLFSLSILKVNWAISVDALSS
LMLIVITTVSLVVHLYSIGYMEHDKGKSRFFSYLSLFTFCMIVLVVSDNFVQLFFGWEGVGLCSYLLIGFWFQKYSANNA
AIKAFVVNRVGDFALLIGIFLIYYTFHSLKFTEVFDTADLLGTQNIRAFCCEFKVTHIICILLFIGCMGKSAQLGLHVWL
PDAMEGPTPVSALIHAATMVTAGIFLVAKCSPLFELSNVARELIVIVGALTAFFAATVAITQNDIKKIIAYSTCSQLGYM
FMACGLSAYNVAIFHLMTHAFFKALLFLGAGNVIHAMHHEQNIQKMGNCWKKIPCTYTLMWIGSLALSGIFPFAGFYSKD
LIIEHAYSTDSFAFVISLVVAFFTAFYSWRLLLLVFHSQKQSKINIHEAPKIMLIPLLILAFGSVFSGVWGANILNITSN
AFWKSSLVVIDEHGVHNFFIKLLPTLASLSGIALAYLIYQYQVIRQIKSKFLLKFLQNKWYFDEVYEFVIIAPIRFISRL
LWKFDVKAIDSFGPNGIVRLVNECSKSSIKLQTGYIFDYAFIMFVTLIIGALYIIGIK
>Mature_617_residues
TYILKLIVFLPLFGSLFAALFRRDVFSQLVTTAGIGISAVLSWYVFLTFSENYHLSLFPLFSLSILKVNWAISVDALSSL
MLIVITTVSLVVHLYSIGYMEHDKGKSRFFSYLSLFTFCMIVLVVSDNFVQLFFGWEGVGLCSYLLIGFWFQKYSANNAA
IKAFVVNRVGDFALLIGIFLIYYTFHSLKFTEVFDTADLLGTQNIRAFCCEFKVTHIICILLFIGCMGKSAQLGLHVWLP
DAMEGPTPVSALIHAATMVTAGIFLVAKCSPLFELSNVARELIVIVGALTAFFAATVAITQNDIKKIIAYSTCSQLGYMF
MACGLSAYNVAIFHLMTHAFFKALLFLGAGNVIHAMHHEQNIQKMGNCWKKIPCTYTLMWIGSLALSGIFPFAGFYSKDL
IIEHAYSTDSFAFVISLVVAFFTAFYSWRLLLLVFHSQKQSKINIHEAPKIMLIPLLILAFGSVFSGVWGANILNITSNA
FWKSSLVVIDEHGVHNFFIKLLPTLASLSGIALAYLIYQYQVIRQIKSKFLLKFLQNKWYFDEVYEFVIIAPIRFISRLL
WKFDVKAIDSFGPNGIVRLVNECSKSSIKLQTGYIFDYAFIMFVTLIIGALYIIGIK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=405, Percent_Identity=42.4691358024691, Blast_Score=302, Evalue=6e-82,
Organism=Escherichia coli, GI1788614, Length=575, Percent_Identity=38.6086956521739, Blast_Score=354, Evalue=9e-99,
Organism=Escherichia coli, GI1788829, Length=343, Percent_Identity=30.0291545189504, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788827, Length=352, Percent_Identity=30.1136363636364, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788831, Length=361, Percent_Identity=28.808864265928, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788613, Length=425, Percent_Identity=24.9411764705882, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI2367154, Length=376, Percent_Identity=22.3404255319149, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI145693160, Length=265, Percent_Identity=24.5283018867925, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 69496; Mature: 69365

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYILKLIVFLPLFGSLFAALFRRDVFSQLVTTAGIGISAVLSWYVFLTFSENYHLSLFP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCEEEEH
LFSLSILKVNWAISVDALSSLMLIVITTVSLVVHLYSIGYMEHDKGKSRFFSYLSLFTFC
HHHEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MIVLVVSDNFVQLFFGWEGVGLCSYLLIGFWFQKYSANNAAIKAFVVNRVGDFALLIGIF
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LIYYTFHSLKFTEVFDTADLLGTQNIRAFCCEFKVTHIICILLFIGCMGKSAQLGLHVWL
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PDAMEGPTPVSALIHAATMVTAGIFLVAKCSPLFELSNVARELIVIVGALTAFFAATVAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQNDIKKIIAYSTCSQLGYMFMACGLSAYNVAIFHLMTHAFFKALLFLGAGNVIHAMHHE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QNIQKMGNCWKKIPCTYTLMWIGSLALSGIFPFAGFYSKDLIIEHAYSTDSFAFVISLVV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCHHHHHHHHHH
AFFTAFYSWRLLLLVFHSQKQSKINIHEAPKIMLIPLLILAFGSVFSGVWGANILNITSN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEECCC
AFWKSSLVVIDEHGVHNFFIKLLPTLASLSGIALAYLIYQYQVIRQIKSKFLLKFLQNKW
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YFDEVYEFVIIAPIRFISRLLWKFDVKAIDSFGPNGIVRLVNECSKSSIKLQTGYIFDYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECHHHHHH
FIMFVTLIIGALYIIGIK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYILKLIVFLPLFGSLFAALFRRDVFSQLVTTAGIGISAVLSWYVFLTFSENYHLSLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCEEEEH
LFSLSILKVNWAISVDALSSLMLIVITTVSLVVHLYSIGYMEHDKGKSRFFSYLSLFTFC
HHHEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MIVLVVSDNFVQLFFGWEGVGLCSYLLIGFWFQKYSANNAAIKAFVVNRVGDFALLIGIF
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LIYYTFHSLKFTEVFDTADLLGTQNIRAFCCEFKVTHIICILLFIGCMGKSAQLGLHVWL
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PDAMEGPTPVSALIHAATMVTAGIFLVAKCSPLFELSNVARELIVIVGALTAFFAATVAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQNDIKKIIAYSTCSQLGYMFMACGLSAYNVAIFHLMTHAFFKALLFLGAGNVIHAMHHE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QNIQKMGNCWKKIPCTYTLMWIGSLALSGIFPFAGFYSKDLIIEHAYSTDSFAFVISLVV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCHHHHHHHHHH
AFFTAFYSWRLLLLVFHSQKQSKINIHEAPKIMLIPLLILAFGSVFSGVWGANILNITSN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEECCC
AFWKSSLVVIDEHGVHNFFIKLLPTLASLSGIALAYLIYQYQVIRQIKSKFLLKFLQNKW
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YFDEVYEFVIIAPIRFISRLLWKFDVKAIDSFGPNGIVRLVNECSKSSIKLQTGYIFDYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECHHHHHH
FIMFVTLIIGALYIIGIK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA