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The map label for this gene is cofE [H]

Identifier: 225630592

GI number: 225630592

Start: 931214

End: 931969

Strand: Reverse

Name: cofE [H]

Synonym: WRi_008600

Alternate gene names: 225630592

Gene position: 931969-931214 (Counterclockwise)

Preceding gene: 225630593

Following gene: 225630590

Centisome position: 64.46

GC content: 37.57

Gene sequence:

>756_bases
ATGCATGTTAAAGCAATTTATACCCACCGCATTGAATGTGGAGAAGTATTAGAGCAGGTTCTTGATCGCTATGTTTCAGA
GTTGCTAGGAGAGGAAGTTGTTCTTGCTATCACATCAAAAATCATTTCTGTTTGTCAAAAACAAGTGGTTTGCAAAACTG
CTTGTTCTAAAGAAGAGCTAATCAAAAGAGAAGCTGATGCAATTGTTGGTGTAGACCATAATTCTTACGGCATCTATTTA
ACGATTAAAGACAATATCTTAATTCCATCTGCTGGTATTGATGAATCAAATGGTAATGAAATGTATATTTTATACCCAAA
GGATGTTCAGAAAACAGCTCTATCAATATGGAACTATCTCAAGACAAAACACTGCATAAAACATCTTGGTATTTTGATTA
CAGATAGTAATGTAACACCTATGCGTCGTGGGGTTACGGGCGTTGCTCTTGGTTGGTGTGGATTTGAACCACTTTATTCC
TATATAGGCAAGCTAGATCTTTATAATCAACCACTGCAAGTAACACAAGTCAATCTTCTTGATGCACTGGCAACATCTGC
TGTTTTAGTTATGGGGGAAGGGGCGGAGCAAACACCAATGGCAATAATTAGTGGTGCACCAAAGATTCACTTTCTTACTC
GTCCGCCAACTGTAGAAGAAGAAAAAAGTGTTAAGATATCTATGGAAGAAGATCTTTACTCTCCTCTCCTCATGAGAGCC
CGTTGGTTAAAGAGGAACTTAAAAATTTGCTATTAG

Upstream 100 bases:

>100_bases
AAAGGTGATGTATATTTCAACTTAACACTTCTTGATGGATGGCATAAAACTATTCTCACCAAAACGAAAGATTATACTAT
ATGTAGGCTAACACGTTAAT

Downstream 100 bases:

>100_bases
ACTTCTTAGAGGTTGTCCGGAAACAAGTAAAAGGCTATAATATCTGAAATTTTAGTACGGGGTAAAGATGAGAAAAAAGT
ATCCAACAGATCTAAGCGAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MHVKAIYTHRIECGEVLEQVLDRYVSELLGEEVVLAITSKIISVCQKQVVCKTACSKEELIKREADAIVGVDHNSYGIYL
TIKDNILIPSAGIDESNGNEMYILYPKDVQKTALSIWNYLKTKHCIKHLGILITDSNVTPMRRGVTGVALGWCGFEPLYS
YIGKLDLYNQPLQVTQVNLLDALATSAVLVMGEGAEQTPMAIISGAPKIHFLTRPPTVEEEKSVKISMEEDLYSPLLMRA
RWLKRNLKICY

Sequences:

>Translated_251_residues
MHVKAIYTHRIECGEVLEQVLDRYVSELLGEEVVLAITSKIISVCQKQVVCKTACSKEELIKREADAIVGVDHNSYGIYL
TIKDNILIPSAGIDESNGNEMYILYPKDVQKTALSIWNYLKTKHCIKHLGILITDSNVTPMRRGVTGVALGWCGFEPLYS
YIGKLDLYNQPLQVTQVNLLDALATSAVLVMGEGAEQTPMAIISGAPKIHFLTRPPTVEEEKSVKISMEEDLYSPLLMRA
RWLKRNLKICY
>Mature_251_residues
MHVKAIYTHRIECGEVLEQVLDRYVSELLGEEVVLAITSKIISVCQKQVVCKTACSKEELIKREADAIVGVDHNSYGIYL
TIKDNILIPSAGIDESNGNEMYILYPKDVQKTALSIWNYLKTKHCIKHLGILITDSNVTPMRRGVTGVALGWCGFEPLYS
YIGKLDLYNQPLQVTQVNLLDALATSAVLVMGEGAEQTPMAIISGAPKIHFLTRPPTVEEEKSVKISMEEDLYSPLLMRA
RWLKRNLKICY

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 28129; Mature: 28129

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVKAIYTHRIECGEVLEQVLDRYVSELLGEEVVLAITSKIISVCQKQVVCKTACSKEEL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IKREADAIVGVDHNSYGIYLTIKDNILIPSAGIDESNGNEMYILYPKDVQKTALSIWNYL
HHHHCCEEEEECCCCEEEEEEEECCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
KTKHCIKHLGILITDSNVTPMRRGVTGVALGWCGFEPLYSYIGKLDLYNQPLQVTQVNLL
HHHHHHHHCCEEEECCCCCHHHHCCCEEEEECCCHHHHHHHHCCHHCCCCCCHHHHHHHH
DALATSAVLVMGEGAEQTPMAIISGAPKIHFLTRPPTVEEEKSVKISMEEDLYSPLLMRA
HHHHHHEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHH
RWLKRNLKICY
HHHHHCCCCCC
>Mature Secondary Structure
MHVKAIYTHRIECGEVLEQVLDRYVSELLGEEVVLAITSKIISVCQKQVVCKTACSKEEL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IKREADAIVGVDHNSYGIYLTIKDNILIPSAGIDESNGNEMYILYPKDVQKTALSIWNYL
HHHHCCEEEEECCCCEEEEEEEECCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
KTKHCIKHLGILITDSNVTPMRRGVTGVALGWCGFEPLYSYIGKLDLYNQPLQVTQVNLL
HHHHHHHHCCEEEECCCCCHHHHCCCEEEEECCCHHHHHHHHCCHHCCCCCCHHHHHHHH
DALATSAVLVMGEGAEQTPMAIISGAPKIHFLTRPPTVEEEKSVKISMEEDLYSPLLMRA
HHHHHHEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHH
RWLKRNLKICY
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]