Click here to switch to the map view.

The map label for this gene is folA [H]

Identifier: 225630593

GI number: 225630593

Start: 931971

End: 932459

Strand: Reverse

Name: folA [H]

Synonym: WRi_008610

Alternate gene names: 225630593

Gene position: 932459-931971 (Counterclockwise)

Preceding gene: 225630594

Following gene: 225630592

Centisome position: 64.49

GC content: 33.74

Gene sequence:

>489_bases
ATGCTAGTTATCGGAATTATGGCTGTTGATCCCAAGGGGGTAATTGGAATAAATAATGGATTGCCCTGGCATTATCCAAG
TGAGTTAGATCATTTTCGTCAGGTGACTGACAAACAAGTTATTGTTATGGGGAGAAAGACATTTGAAACTATGCCTCAAG
ACATATTAGGGAATCGTATTCCTATTGTTTTTTCTCGTGACAAATTAAATTCTTGTTTTAACAGAGGAGTAAAATGCACT
ATTATTTCTTCTATGCAAGAGTTTTTGTCAATCCAAAGTAATTGGAGTTACCCTCAAATCTTTGTGATTGGTGGAGCACA
AATAGCGCATCTTTTTTTAGAATATAATCTAATTTCAGAGTTCATCATGACAGAAATTTACAAACCCTATAAAGGTGATG
TATATTTCAACTTAACACTTCTTGATGGATGGCATAAAACTATTCTCACCAAAACGAAAGATTATACTATATGTAGGCTA
ACACGTTAA

Upstream 100 bases:

>100_bases
TTGCTTTATCATCTGCATTGCACAACAAGGTTGATTTCCTTCGAGTGCATAATGTGCGTGATCACATGCGATTTTTTGTA
GCTCAAGCTGCTTTACAGGG

Downstream 100 bases:

>100_bases
TATGCATGTTAAAGCAATTTATACCCACCGCATTGAATGTGGAGAAGTATTAGAGCAGGTTCTTGATCGCTATGTTTCAG
AGTTGCTAGGAGAGGAAGTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MLVIGIMAVDPKGVIGINNGLPWHYPSELDHFRQVTDKQVIVMGRKTFETMPQDILGNRIPIVFSRDKLNSCFNRGVKCT
IISSMQEFLSIQSNWSYPQIFVIGGAQIAHLFLEYNLISEFIMTEIYKPYKGDVYFNLTLLDGWHKTILTKTKDYTICRL
TR

Sequences:

>Translated_162_residues
MLVIGIMAVDPKGVIGINNGLPWHYPSELDHFRQVTDKQVIVMGRKTFETMPQDILGNRIPIVFSRDKLNSCFNRGVKCT
IISSMQEFLSIQSNWSYPQIFVIGGAQIAHLFLEYNLISEFIMTEIYKPYKGDVYFNLTLLDGWHKTILTKTKDYTICRL
TR
>Mature_162_residues
MLVIGIMAVDPKGVIGINNGLPWHYPSELDHFRQVTDKQVIVMGRKTFETMPQDILGNRIPIVFSRDKLNSCFNRGVKCT
IISSMQEFLSIQSNWSYPQIFVIGGAQIAHLFLEYNLISEFIMTEIYKPYKGDVYFNLTLLDGWHKTILTKTKDYTICRL
TR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17505899, Length=144, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324810, Length=121, Percent_Identity=33.8842975206612, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18711; Mature: 18711

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVIGIMAVDPKGVIGINNGLPWHYPSELDHFRQVTDKQVIVMGRKTFETMPQDILGNRI
CEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECHHHHCCCHHHCCCCC
PIVFSRDKLNSCFNRGVKCTIISSMQEFLSIQSNWSYPQIFVIGGAQIAHLFLEYNLISE
CEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHH
FIMTEIYKPYKGDVYFNLTLLDGWHKTILTKTKDYTICRLTR
HHHHHHCCCCCCCEEEEEEEECCCCHHHEECCCCCEEEEECC
>Mature Secondary Structure
MLVIGIMAVDPKGVIGINNGLPWHYPSELDHFRQVTDKQVIVMGRKTFETMPQDILGNRI
CEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECHHHHCCCHHHCCCCC
PIVFSRDKLNSCFNRGVKCTIISSMQEFLSIQSNWSYPQIFVIGGAQIAHLFLEYNLISE
CEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHH
FIMTEIYKPYKGDVYFNLTLLDGWHKTILTKTKDYTICRLTR
HHHHHHCCCCCCCEEEEEEEECCCCHHHEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7574597; 11337471 [H]