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The map label for this gene is radA [H]

Identifier: 225630590

GI number: 225630590

Start: 927197

End: 928540

Strand: Reverse

Name: radA [H]

Synonym: WRi_008560

Alternate gene names: 225630590

Gene position: 928540-927197 (Counterclockwise)

Preceding gene: 225630592

Following gene: 225630588

Centisome position: 64.22

GC content: 37.87

Gene sequence:

>1344_bases
ATGAAAACTGTATATGTATGTCAATCCTGTGGTCACAGCACTGGTAGGTGGGTAGGGAAGTGTATTGCATGTGACAATTG
GGATACAGTTGTTGAGGAAATAGCAGTAAAAACGGGCAAAAGAAGTATATCTGCGCCAATTTTAATAAAGACGTTATCGG
GTAGCGAAATTATCACTCCAAGTCGTTTTTTAACAGGAATAGAAGAATTAGATAGAGTTTTTGGTGGGGGTATCGTTCAA
GGTGCTAGCATTTTAATTGGTGGCGAACCTGGAATTGGGAAATCTACCCTTTTGCTTAGAATTGCAGCTAATGTTGTGCA
ACTTTCCTCTTTTGAATGTCTTTATGTATCTGGCGAGGAATCTTTAGAGCAGGTGAGTCTCAGAGCAAAGCGTCTTAAGA
TAAATGAACCTAAAATTAAGCTTTTATCTACAGTGTCTTTAACTGATGTAGTAGCAACAATAAAGGAAAATAAAAGTATT
AAATTCTTAGTAATTGACTCTATACAAACAATGTATGATAGCAGAATTACATCGGCACCAGGCACTGTAACTCAGGTCCG
CACTTGCGCACATGAATTAACTATTCTTGCGAAACAATATGGTATTTCCCTCTTAATAGTTGGCCATATAACTAAGGATG
GACAAATAGCTGGACCTAAAACTTTGGAGCATATGGTGGATACGGTATTATATTTTGAAGGTGAAAATAGTAATCAATAT
CGCATTTTACGCACTATTAAAAATAGATTTGGCCCTGCAAATGAAATTGGTGTTTTTGAGATGTCTGAAGCAGGACTTGT
GCCTGTTGATAACCCATCATCATTGTTTCTGATGGCCCATGACAGAGAAGTAGTTGGCAGCGCCGTATTTGCAGGAATTG
AAGGTTCAAGACCAATTTTAATGGAAGTGCAAGCATTAATAGCAGGGACTAATATGGCAACCCCAAGAAGGGCAGTAGTT
GGGTGGGATATTAATCGATTGGCTATGATCATCGCGGTGCTAAATGCTTGTTGCAAAATGTTTTTGCATGATAAAGAGGT
ATACCTAAACATTGCAGGGGGACTCAAAATACAGGAACCATCGGCTGATCTTGCTGTTGCTGCTTCCCTTATTTCAAGTG
TAGTAAATTTACCGCTGCCAACTTCTTCAATAATCTGCGGTGAAGTTGCACTTTCAGGAGAAATTAGGAATGTCTCGCAT
GCTGATCTCAGACTAAAAGAAGCACAAAAGTTAGGATTCAAAAAAGCAATTATGCCTAAAAATGGTAATTACTCTTCTCA
TGATATTGAGATAATTAAGCTTGGTCACGTAAGGGAATTAAGAGAAATTTTTAATTACAATTAA

Upstream 100 bases:

>100_bases
GCGGTATGACATAGGGAACCCTTTCTTGGGTTAGCTATAGATTTACTTCGCATATTCAGCTAATTTTTATTACAATTACA
ACAAAATAATTTTGGCTTTG

Downstream 100 bases:

>100_bases
ATAACGCTTCAGCAAATTCTTTGCTAGTAAACTCCCTTAAGTCCTCTATATTTTCGCCAATTCCTATAGCATGTAACTTT
ATCTTATAAGCTTCTGCAAG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKTVYVCQSCGHSTGRWVGKCIACDNWDTVVEEIAVKTGKRSISAPILIKTLSGSEIITPSRFLTGIEELDRVFGGGIVQ
GASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIKENKSI
KFLVIDSIQTMYDSRITSAPGTVTQVRTCAHELTILAKQYGISLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENSNQY
RILRTIKNRFGPANEIGVFEMSEAGLVPVDNPSSLFLMAHDREVVGSAVFAGIEGSRPILMEVQALIAGTNMATPRRAVV
GWDINRLAMIIAVLNACCKMFLHDKEVYLNIAGGLKIQEPSADLAVAASLISSVVNLPLPTSSIICGEVALSGEIRNVSH
ADLRLKEAQKLGFKKAIMPKNGNYSSHDIEIIKLGHVRELREIFNYN

Sequences:

>Translated_447_residues
MKTVYVCQSCGHSTGRWVGKCIACDNWDTVVEEIAVKTGKRSISAPILIKTLSGSEIITPSRFLTGIEELDRVFGGGIVQ
GASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIKENKSI
KFLVIDSIQTMYDSRITSAPGTVTQVRTCAHELTILAKQYGISLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENSNQY
RILRTIKNRFGPANEIGVFEMSEAGLVPVDNPSSLFLMAHDREVVGSAVFAGIEGSRPILMEVQALIAGTNMATPRRAVV
GWDINRLAMIIAVLNACCKMFLHDKEVYLNIAGGLKIQEPSADLAVAASLISSVVNLPLPTSSIICGEVALSGEIRNVSH
ADLRLKEAQKLGFKKAIMPKNGNYSSHDIEIIKLGHVRELREIFNYN
>Mature_447_residues
MKTVYVCQSCGHSTGRWVGKCIACDNWDTVVEEIAVKTGKRSISAPILIKTLSGSEIITPSRFLTGIEELDRVFGGGIVQ
GASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIKENKSI
KFLVIDSIQTMYDSRITSAPGTVTQVRTCAHELTILAKQYGISLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENSNQY
RILRTIKNRFGPANEIGVFEMSEAGLVPVDNPSSLFLMAHDREVVGSAVFAGIEGSRPILMEVQALIAGTNMATPRRAVV
GWDINRLAMIIAVLNACCKMFLHDKEVYLNIAGGLKIQEPSADLAVAASLISSVVNLPLPTSSIICGEVALSGEIRNVSH
ADLRLKEAQKLGFKKAIMPKNGNYSSHDIEIIKLGHVRELREIFNYN

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=428, Percent_Identity=43.6915887850467, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 48547; Mature: 48547

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVYVCQSCGHSTGRWVGKCIACDNWDTVVEEIAVKTGKRSISAPILIKTLSGSEIITP
CCEEEEECCCCCCCCCHHEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEECH
SRFLTGIEELDRVFGGGIVQGASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEE
HHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHEECCCEEEEECCHH
SLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIKENKSIKFLVIDSIQTMYDSRITSAP
HHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCC
GTVTQVRTCAHELTILAKQYGISLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENSNQY
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHEEEEEECCCCCCC
RILRTIKNRFGPANEIGVFEMSEAGLVPVDNPSSLFLMAHDREVVGSAVFAGIEGSRPIL
HHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCEEEEEECCHHHHHHHHEECCCCCCCHH
MEVQALIAGTNMATPRRAVVGWDINRLAMIIAVLNACCKMFLHDKEVYLNIAGGLKIQEP
HHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECC
SADLAVAASLISSVVNLPLPTSSIICGEVALSGEIRNVSHADLRLKEAQKLGFKKAIMPK
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCC
NGNYSSHDIEIIKLGHVRELREIFNYN
CCCCCCCCEEEEEECCHHHHHHHHCCC
>Mature Secondary Structure
MKTVYVCQSCGHSTGRWVGKCIACDNWDTVVEEIAVKTGKRSISAPILIKTLSGSEIITP
CCEEEEECCCCCCCCCHHEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEECH
SRFLTGIEELDRVFGGGIVQGASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEE
HHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHEECCCEEEEECCHH
SLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIKENKSIKFLVIDSIQTMYDSRITSAP
HHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCC
GTVTQVRTCAHELTILAKQYGISLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENSNQY
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHEEEEEECCCCCCC
RILRTIKNRFGPANEIGVFEMSEAGLVPVDNPSSLFLMAHDREVVGSAVFAGIEGSRPIL
HHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCEEEEEECCHHHHHHHHEECCCCCCCHH
MEVQALIAGTNMATPRRAVVGWDINRLAMIIAVLNACCKMFLHDKEVYLNIAGGLKIQEP
HHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECC
SADLAVAASLISSVVNLPLPTSSIICGEVALSGEIRNVSHADLRLKEAQKLGFKKAIMPK
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEECCC
NGNYSSHDIEIIKLGHVRELREIFNYN
CCCCCCCCEEEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA