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The map label for this gene is ctaB [H]

Identifier: 225630265

GI number: 225630265

Start: 525986

End: 526873

Strand: Reverse

Name: ctaB [H]

Synonym: WRi_004810

Alternate gene names: 225630265

Gene position: 526873-525986 (Counterclockwise)

Preceding gene: 225630266

Following gene: 225630264

Centisome position: 36.44

GC content: 36.15

Gene sequence:

>888_bases
ATGTACACAAGTGTTTTGCTAAATGTTGAATCAACAATACTGGATTTTTGGCGTTTGCTGAAGCCAAGGATAATGTATCT
TGTGGTATTTACTGCAGTTGCTGGTATGGTTACTGCACCAGGTAGTATGCACCCTTTTCTAGCACTAATATCTCTTATAT
GTGTTGCTCTTGGCTCAGGATCTGCAGGTGCTATAAATATGTGGTATGACAGAGACATAGATCTGCTCATGGAAAGGACA
AAAAACCGCCCTATACCTTCAGGTAGAGTTTCTGCAGAAAGTGCGCTTGAATTTGGTATAACTCTTGGAATATTGTCAGT
ATTTATCATGGCAATAGCAGTAAACTATATTTCTGCTGCTTTGCTTGCTGTTAGCATATTATTTTACGTTTTCATATATA
CAATTTGGCTAAAAAGGCGTACTCCGCAGAATATCGTTATCGGTGGTGCAGCAGGTGCTTTCCCTCCAATGATTGGCTGG
GCAGCTGTGACTAATTCTGTAAGTTGGGAAAGTTTCATTTTATTTTTAGTAATTTTTATGTGGACTCCACCACACTTTTG
GGCCCTATCTTTAAATAAGTCTGAAGACTATGCAAAGGCATCAATTCCAATGTTCAATATTGTTTATGGTCCGGAAAAAA
CAAGAAAATATATATTAATTTATAGTGTTTTGCTGGTGCTAACTAGCTTGCTTCCGGCTCTATTTTTGAAAAAGGCTTTA
CTTTATCTAAGCATGGCAATTTTTGCAGGTTGCATCTTTATTTGGTACGCTATATCAATTCTCAGATTCAAGAACCACAA
CTCACAGAAACAAATGTTTTCTTACTCAATTTCTTATCTATTTTCCCTATTCGCCAGTATTATTTTTTGTTCTATTGATT
TATTTTAA

Upstream 100 bases:

>100_bases
AAAGCTGGAGATAATCCTTGGGGAGGTGACACCTTGGAATGGACGGTATCTTCACCACCGCCTTTTCATACTTTCGAAAA
ACCACCAGTGGTAAAATAGA

Downstream 100 bases:

>100_bases
TTATGAAAGAACGGCAGAAGCATAAAAACTGTTTTTTACTTTTCCTTTTGGTAATGCTCGTTGTCACACTTTTTTTTGTT
TCTATAATAAAATTTAAAGG

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MYTSVLLNVESTILDFWRLLKPRIMYLVVFTAVAGMVTAPGSMHPFLALISLICVALGSGSAGAINMWYDRDIDLLMERT
KNRPIPSGRVSAESALEFGITLGILSVFIMAIAVNYISAALLAVSILFYVFIYTIWLKRRTPQNIVIGGAAGAFPPMIGW
AAVTNSVSWESFILFLVIFMWTPPHFWALSLNKSEDYAKASIPMFNIVYGPEKTRKYILIYSVLLVLTSLLPALFLKKAL
LYLSMAIFAGCIFIWYAISILRFKNHNSQKQMFSYSISYLFSLFASIIFCSIDLF

Sequences:

>Translated_295_residues
MYTSVLLNVESTILDFWRLLKPRIMYLVVFTAVAGMVTAPGSMHPFLALISLICVALGSGSAGAINMWYDRDIDLLMERT
KNRPIPSGRVSAESALEFGITLGILSVFIMAIAVNYISAALLAVSILFYVFIYTIWLKRRTPQNIVIGGAAGAFPPMIGW
AAVTNSVSWESFILFLVIFMWTPPHFWALSLNKSEDYAKASIPMFNIVYGPEKTRKYILIYSVLLVLTSLLPALFLKKAL
LYLSMAIFAGCIFIWYAISILRFKNHNSQKQMFSYSISYLFSLFASIIFCSIDLF
>Mature_295_residues
MYTSVLLNVESTILDFWRLLKPRIMYLVVFTAVAGMVTAPGSMHPFLALISLICVALGSGSAGAINMWYDRDIDLLMERT
KNRPIPSGRVSAESALEFGITLGILSVFIMAIAVNYISAALLAVSILFYVFIYTIWLKRRTPQNIVIGGAAGAFPPMIGW
AAVTNSVSWESFILFLVIFMWTPPHFWALSLNKSEDYAKASIPMFNIVYGPEKTRKYILIYSVLLVLTSLLPALFLKKAL
LYLSMAIFAGCIFIWYAISILRFKNHNSQKQMFSYSISYLFSLFASIIFCSIDLF

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group [H]

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI17921982, Length=204, Percent_Identity=34.3137254901961, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786631, Length=268, Percent_Identity=27.9850746268657, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71998235, Length=198, Percent_Identity=30.8080808080808, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325085, Length=196, Percent_Identity=34.6938775510204, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921064, Length=276, Percent_Identity=30.7971014492754, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006369
- InterPro:   IPR000537 [H]

Pfam domain/function: PF01040 UbiA [H]

EC number: 2.5.1.- [C]

Molecular weight: Translated: 33125; Mature: 33125

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTSVLLNVESTILDFWRLLKPRIMYLVVFTAVAGMVTAPGSMHPFLALISLICVALGSG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
SAGAINMWYDRDIDLLMERTKNRPIPSGRVSAESALEFGITLGILSVFIMAIAVNYISAA
CCCEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAVSILFYVFIYTIWLKRRTPQNIVIGGAAGAFPPMIGWAAVTNSVSWESFILFLVIFM
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
WTPPHFWALSLNKSEDYAKASIPMFNIVYGPEKTRKYILIYSVLLVLTSLLPALFLKKAL
HCCCCEEEEEECCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLSMAIFAGCIFIWYAISILRFKNHNSQKQMFSYSISYLFSLFASIIFCSIDLF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTSVLLNVESTILDFWRLLKPRIMYLVVFTAVAGMVTAPGSMHPFLALISLICVALGSG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
SAGAINMWYDRDIDLLMERTKNRPIPSGRVSAESALEFGITLGILSVFIMAIAVNYISAA
CCCEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAVSILFYVFIYTIWLKRRTPQNIVIGGAAGAFPPMIGWAAVTNSVSWESFILFLVIFM
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
WTPPHFWALSLNKSEDYAKASIPMFNIVYGPEKTRKYILIYSVLLVLTSLLPALFLKKAL
HCCCCEEEEEECCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLSMAIFAGCIFIWYAISILRFKNHNSQKQMFSYSISYLFSLFASIIFCSIDLF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA