Click here to switch to the map view.

The map label for this gene is rnhA [H]

Identifier: 225630264

GI number: 225630264

Start: 525372

End: 525809

Strand: Reverse

Name: rnhA [H]

Synonym: WRi_004800

Alternate gene names: 225630264

Gene position: 525809-525372 (Counterclockwise)

Preceding gene: 225630265

Following gene: 225630263

Centisome position: 36.37

GC content: 35.39

Gene sequence:

>438_bases
ATGAGCAAAAAGGAAGTGGCAATATATACGGATGGGGCGTGTTCTGGTAATCCTGGAGCGGGAGGATGGGCAGCTATCAT
ATTATTTCAAGATTATAGAAAAGACATTTATGGTAGAGAAGAAAATACCACAAACAATAAAATGGAGTTAACAGCGGTGA
TCAATGGGCTGAAAGTATTGAAATTTTCTTGTAATATTAATTTATATACGGATAGTCTTTATGTTAAACATGGCATAACA
GAGTGGATAAATAAGTGGAAAATGAATGGCTGGAAAACAAGTAATAAAAAATCAGTAAAAAATATGGAATTGTGGAAAGA
GCTAGACAACGTTGCTTCACAACACGAAATTGATTGGAAGTGGGTTAAAGCACACAGTGGCGATAAGTATAATGAGGAGG
CTGATAGTTTAGCGAGAAAAGCAATAATCGATGCTTAA

Upstream 100 bases:

>100_bases
TGTTTCTATAATAAAATTTAAAGGTGCAGCTTAAACTACCTTTAGAAATTGTGCTTTCATAGAAGCAACTATATACTTTA
TTAATTCATTACAGTTAAAG

Downstream 100 bases:

>100_bases
AAAAATTACTGTATTATTTTCCGTAGCTTTATTGTTCATATTGTGCTTCTTTATTTTTTTAAAGCCTTTAGAAATCAATA
TTAATTATATTAATTTTTAT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MSKKEVAIYTDGACSGNPGAGGWAAIILFQDYRKDIYGREENTTNNKMELTAVINGLKVLKFSCNINLYTDSLYVKHGIT
EWINKWKMNGWKTSNKKSVKNMELWKELDNVASQHEIDWKWVKAHSGDKYNEEADSLARKAIIDA

Sequences:

>Translated_145_residues
MSKKEVAIYTDGACSGNPGAGGWAAIILFQDYRKDIYGREENTTNNKMELTAVINGLKVLKFSCNINLYTDSLYVKHGIT
EWINKWKMNGWKTSNKKSVKNMELWKELDNVASQHEIDWKWVKAHSGDKYNEEADSLARKAIIDA
>Mature_144_residues
SKKEVAIYTDGACSGNPGAGGWAAIILFQDYRKDIYGREENTTNNKMELTAVINGLKVLKFSCNINLYTDSLYVKHGITE
WINKWKMNGWKTSNKKSVKNMELWKELDNVASQHEIDWKWVKAHSGDKYNEEADSLARKAIIDA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=145, Percent_Identity=38.6206896551724, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786408, Length=142, Percent_Identity=50, Blast_Score=155, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI115532542, Length=144, Percent_Identity=34.0277777777778, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17505482, Length=134, Percent_Identity=32.089552238806, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=36.986301369863, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=36.986301369863, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16549; Mature: 16418

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKEVAIYTDGACSGNPGAGGWAAIILFQDYRKDIYGREENTTNNKMELTAVINGLKVL
CCCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCEEEHHHHCCEEEE
KFSCNINLYTDSLYVKHGITEWINKWKMNGWKTSNKKSVKNMELWKELDNVASQHEIDWK
EEECCEEEEECCEEEHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
WVKAHSGDKYNEEADSLARKAIIDA
EEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKEVAIYTDGACSGNPGAGGWAAIILFQDYRKDIYGREENTTNNKMELTAVINGLKVL
CCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCEEEHHHHCCEEEE
KFSCNINLYTDSLYVKHGITEWINKWKMNGWKTSNKKSVKNMELWKELDNVASQHEIDWK
EEECCEEEEECCEEEHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
WVKAHSGDKYNEEADSLARKAIIDA
EEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA