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The map label for this gene is yaaJ [C]

Identifier: 225630242

GI number: 225630242

Start: 488612

End: 489955

Strand: Reverse

Name: yaaJ [C]

Synonym: WRi_004550

Alternate gene names: 225630242

Gene position: 489955-488612 (Counterclockwise)

Preceding gene: 225630244

Following gene: 225630241

Centisome position: 33.89

GC content: 33.85

Gene sequence:

>1344_bases
ATGGATACAGTAAAGTTTGTTTTATTACTGCCAACAATATTACTCATGCTAGTTGCTGGTGTTTACCTATCGATTAAACT
AAAATGGCTACAGGTATTTAGATTACCATACGCCCTTTCACTCCTTGGAGCTAAAAAAGGAGAAAATAAATTTTCTTCTA
TAGCCGCTCTGTTTACAATTTTAGGGGGAAATTTAGGAGTAGGAAATATTTCAGGAACTGCTGTTGCTCTAAAAACCGGA
GGACCGGGCTCTATTTTGTGGATGGCGATAATTATTGTTATCACTTCTGTGATAAAATATGTCACTTGTTATCTAAGTAT
AAAAACCAGAAAGGAAAAGAACGGACGGTTTATAGGTGGCCCTGTAGCCTACATGGCTGATGCATTTAACTCTAGAAAAG
CTACAATTGTGTTTCTAGTTGTTATGATAATGGTTTCAATAACAGTTGGTAACCTTGTTCAGGTAAATTCTCTATCAATA
CCACTTAACATGATAGATGTGCCTGTAGTCATTGGTGGCATTGTAATGGCCATAATATTTTTTGTTGTTGCAGCTCTCAG
CTTAAAAAAAATTAAAATTTTTATATCAGCTATGATACCGATAATGACGGTAAGTTACCTCACACTTTGCGGTATCATAT
TATTTAAATTTAGTGAAAATATTCTTCCTTCTCTAAAACTAATAACAAGCAGTTTTTTTACAACCAGTAGCTTTAACTCT
GGTTTATCTCTAGGTTTGATTCTAGAAATGTTGACTATTATTCAAGTAGGAACTTTGCGAGGTATTTTTGCAACAGATAT
AGGGCTTGGCCTTGAAGGAATCGTACATTCTTCAATTGTTCCTAAGAAAAATAATAATAAATTTATTATTGAGCAGAGTC
TAATTACGATAATATCGCCTTTTATAGTTGCGTTTATAGTGTTTATTACAACAATGGTACTGCTTGTCACAGATTCTTGG
GTCACTGATTTAGAGAGCACTAACATGTGCATATTTGCTTTTAGAAAGGCAATGAATTGGCCTTATATTGACTATTTAAT
TATGGCCATAATGTTTTGTTTTGCTTTTACTACTATTTTTACTTGGTTTTTTTGCTCAAAACAGACAATACGCTATGTGT
CTATGGATAATAAATACACTAAAATCTGGGTTGTAATTTTTACCTTGATTATTCCGCTTGGTGCGATAGGTAAAGTTCAA
TTGCTATGGGATGTTGCTGATATTTCGATCGCCGCTTTGTTGTTTATCAACATACTCGCTATACTCAAGCTAACCTCTCA
AGATCCAGAAGTGTTTACTATGAGTAGCAGATATTTGAAGTTGCAAAAACAGAGCTATAGCTAA

Upstream 100 bases:

>100_bases
ACAAGGTTTCTAGAATGACGGAAACGAAGTCATACATAGAAAATAGGTTAGGTATTTTCAATTTTTGTTGTATACTCAAA
TAAAAATCTCACTTTTACAG

Downstream 100 bases:

>100_bases
AGCAATTTAGGCTATGCAGCTGTTGTGATATAAGAGTGATTTCCGCCAAGAAGGATGTCATCCCAGTGCCCAGACACTGG
GATCCAGGAATCTTGATTGG

Product: Na+/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDTVKFVLLLPTILLMLVAGVYLSIKLKWLQVFRLPYALSLLGAKKGENKFSSIAALFTILGGNLGVGNISGTAVALKTG
GPGSILWMAIIIVITSVIKYVTCYLSIKTRKEKNGRFIGGPVAYMADAFNSRKATIVFLVVMIMVSITVGNLVQVNSLSI
PLNMIDVPVVIGGIVMAIIFFVVAALSLKKIKIFISAMIPIMTVSYLTLCGIILFKFSENILPSLKLITSSFFTTSSFNS
GLSLGLILEMLTIIQVGTLRGIFATDIGLGLEGIVHSSIVPKKNNNKFIIEQSLITIISPFIVAFIVFITTMVLLVTDSW
VTDLESTNMCIFAFRKAMNWPYIDYLIMAIMFCFAFTTIFTWFFCSKQTIRYVSMDNKYTKIWVVIFTLIIPLGAIGKVQ
LLWDVADISIAALLFINILAILKLTSQDPEVFTMSSRYLKLQKQSYS

Sequences:

>Translated_447_residues
MDTVKFVLLLPTILLMLVAGVYLSIKLKWLQVFRLPYALSLLGAKKGENKFSSIAALFTILGGNLGVGNISGTAVALKTG
GPGSILWMAIIIVITSVIKYVTCYLSIKTRKEKNGRFIGGPVAYMADAFNSRKATIVFLVVMIMVSITVGNLVQVNSLSI
PLNMIDVPVVIGGIVMAIIFFVVAALSLKKIKIFISAMIPIMTVSYLTLCGIILFKFSENILPSLKLITSSFFTTSSFNS
GLSLGLILEMLTIIQVGTLRGIFATDIGLGLEGIVHSSIVPKKNNNKFIIEQSLITIISPFIVAFIVFITTMVLLVTDSW
VTDLESTNMCIFAFRKAMNWPYIDYLIMAIMFCFAFTTIFTWFFCSKQTIRYVSMDNKYTKIWVVIFTLIIPLGAIGKVQ
LLWDVADISIAALLFINILAILKLTSQDPEVFTMSSRYLKLQKQSYS
>Mature_447_residues
MDTVKFVLLLPTILLMLVAGVYLSIKLKWLQVFRLPYALSLLGAKKGENKFSSIAALFTILGGNLGVGNISGTAVALKTG
GPGSILWMAIIIVITSVIKYVTCYLSIKTRKEKNGRFIGGPVAYMADAFNSRKATIVFLVVMIMVSITVGNLVQVNSLSI
PLNMIDVPVVIGGIVMAIIFFVVAALSLKKIKIFISAMIPIMTVSYLTLCGIILFKFSENILPSLKLITSSFFTTSSFNS
GLSLGLILEMLTIIQVGTLRGIFATDIGLGLEGIVHSSIVPKKNNNKFIIEQSLITIISPFIVAFIVFITTMVLLVTDSW
VTDLESTNMCIFAFRKAMNWPYIDYLIMAIMFCFAFTTIFTWFFCSKQTIRYVSMDNKYTKIWVVIFTLIIPLGAIGKVQ
LLWDVADISIAALLFINILAILKLTSQDPEVFTMSSRYLKLQKQSYS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=444, Percent_Identity=23.8738738738739, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49373; Mature: 49373

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVKFVLLLPTILLMLVAGVYLSIKLKWLQVFRLPYALSLLGAKKGENKFSSIAALFTI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LGGNLGVGNISGTAVALKTGGPGSILWMAIIIVITSVIKYVTCYLSIKTRKEKNGRFIGG
HCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEECC
PVAYMADAFNSRKATIVFLVVMIMVSITVGNLVQVNSLSIPLNMIDVPVVIGGIVMAIIF
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
FVVAALSLKKIKIFISAMIPIMTVSYLTLCGIILFKFSENILPSLKLITSSFFTTSSFNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSLGLILEMLTIIQVGTLRGIFATDIGLGLEGIVHSSIVPKKNNNKFIIEQSLITIISP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHH
FIVAFIVFITTMVLLVTDSWVTDLESTNMCIFAFRKAMNWPYIDYLIMAIMFCFAFTTIF
HHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TWFFCSKQTIRYVSMDNKYTKIWVVIFTLIIPLGAIGKVQLLWDVADISIAALLFINILA
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
ILKLTSQDPEVFTMSSRYLKLQKQSYS
HHHHCCCCCCEEEECHHHHHHHHCCCC
>Mature Secondary Structure
MDTVKFVLLLPTILLMLVAGVYLSIKLKWLQVFRLPYALSLLGAKKGENKFSSIAALFTI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LGGNLGVGNISGTAVALKTGGPGSILWMAIIIVITSVIKYVTCYLSIKTRKEKNGRFIGG
HCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEECC
PVAYMADAFNSRKATIVFLVVMIMVSITVGNLVQVNSLSIPLNMIDVPVVIGGIVMAIIF
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
FVVAALSLKKIKIFISAMIPIMTVSYLTLCGIILFKFSENILPSLKLITSSFFTTSSFNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSLGLILEMLTIIQVGTLRGIFATDIGLGLEGIVHSSIVPKKNNNKFIIEQSLITIISP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHH
FIVAFIVFITTMVLLVTDSWVTDLESTNMCIFAFRKAMNWPYIDYLIMAIMFCFAFTTIF
HHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TWFFCSKQTIRYVSMDNKYTKIWVVIFTLIIPLGAIGKVQLLWDVADISIAALLFINILA
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
ILKLTSQDPEVFTMSSRYLKLQKQSYS
HHHHCCCCCCEEEECHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]