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The map label for this gene is gltX [H]

Identifier: 225630127

GI number: 225630127

Start: 331961

End: 333391

Strand: Reverse

Name: gltX [H]

Synonym: WRi_003090

Alternate gene names: 225630127

Gene position: 333391-331961 (Counterclockwise)

Preceding gene: 225630130

Following gene: 225630126

Centisome position: 23.06

GC content: 39.83

Gene sequence:

>1431_bases
ATGCTAGACGTAGTCACGAGATTCGCTCCATCACCAACAGGGTTTTTGCACATTGGTGGGGCTCGTACTGCGTTATTTAA
TTGGCTCTATGCAAAGCATCATGGTGGCAGGTTTTTGCTAAGAATTGAGGACACCGACAGAAAACGTTCAACGAAAGAAG
CAATTGATGCAATTATAGAAGGGCTAAGATGGCTCGGTGTGAGCTATGATGGGGAAATAGTATATCAGTCAAAAAGAATA
GAGCGGCATAAAGAAGTAGCAAACCTTTTAGTTGAGAAGGGTAGGGCGTATCACTGTTATTGCCCTGAGGATGAAGTTGC
AGAAAAGAAAATAAAAGCAAGAGAAGAGGGCAAGATATACAAGCATAAATGTACTACAAAACCTCCTTCTTGTCATCCAA
GTAGCCGACACTGGGATCCAGAAGAAAATATGTGGTCACGCGCTGGAATGACATCGGGTGTGAGGTCAGTTATCCGTTTC
AAAGTGCCAGACTCACAAGAGATCGTTATTGATGATAAAATATACGGCCAAATCAAAGTAAATAGCAACCAGCTTGATGA
TATAGTGATCTTACGCTCTGACAACACTCCAACATATATTTTTGCTGTCGTAATTGACGATCATGATGCTGGAATAACTG
ATATAATACGCGGTTCTGACCATCTCACTAATACCTTTAAACATCTGCTAATATACCAGGCTCTTGATTTTGACATTCCA
CGCTTTGCACATGTGCCGCTTATCCATGGGGAAGATGGGAATAAGCTATCCAAAAGGCATGGTGCCACAAGCGTTTGCGA
TTATGAAAAGATGGGAATATTGCCAAAAGCGATGCGTAATTACTTGCTGAGACTTGGCTGGAGTCACGGCAACGATGAGA
TTATTAGCGATGAGCAAGCAATAGAGTGGTTTAATTTAGAAAGTATTGGTCGTTCACCTGCACGGCTCGATTTCAAAAAA
CTGGAGCATTTGAATAACCACTATATTAGTAATATGAGCAATGAAGATATTCTGACTCTAATGCTTAGAGAAAATACTCT
AACTGACAAAAAAAAGGGCTATTTACTGCAGGGACTGACAGAGCTAAAAAAAAGAGCAAACTACCTGACCGAATTATTGG
ATTTAGCAAAATTTTATATTCAAGATCCGCCACTTGATTTAAGTGAAGAGGCTGAGCAAATTGTCAAATCTAACCTCGAT
ATAATCAAGTTACTCGCGTCATTTCTGTCAGAGATCGGTGATGAAAATTGGAATAAGGGTTTTTTATCTTCTCAGATCAA
GGAATGTGCAAAATTGCACGATATGAAAATAAGCGATGTATACCACTCATTACGCGCCCCTATAACTGGAGTAATGGATG
CACCTGGAATCATTGATATCATGGTGATTCTTGGTAAAGATGAGTGTATAAGAAGGTTACAAGCAATTTAA

Upstream 100 bases:

>100_bases
TAAAAATAAAGAAAATTTAATCTCTCAGGATAAATAAATTCTACATTGTTACAAACGTGGATTTTAGGTATCATAGCCTA
TACTTTACTGTAACAATAAC

Downstream 100 bases:

>100_bases
ATGAAATAAGAAAATTTCTTGACAAGCTTTGCCAGCCCCTTTATCATGACAGTGAAGCTATTGTATTTGCTTTCGCCAAT
CTGCAGATTAAAAGGTAAGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MLDVVTRFAPSPTGFLHIGGARTALFNWLYAKHHGGRFLLRIEDTDRKRSTKEAIDAIIEGLRWLGVSYDGEIVYQSKRI
ERHKEVANLLVEKGRAYHCYCPEDEVAEKKIKAREEGKIYKHKCTTKPPSCHPSSRHWDPEENMWSRAGMTSGVRSVIRF
KVPDSQEIVIDDKIYGQIKVNSNQLDDIVILRSDNTPTYIFAVVIDDHDAGITDIIRGSDHLTNTFKHLLIYQALDFDIP
RFAHVPLIHGEDGNKLSKRHGATSVCDYEKMGILPKAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKK
LEHLNNHYISNMSNEDILTLMLRENTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQDPPLDLSEEAEQIVKSNLD
IIKLLASFLSEIGDENWNKGFLSSQIKECAKLHDMKISDVYHSLRAPITGVMDAPGIIDIMVILGKDECIRRLQAI

Sequences:

>Translated_476_residues
MLDVVTRFAPSPTGFLHIGGARTALFNWLYAKHHGGRFLLRIEDTDRKRSTKEAIDAIIEGLRWLGVSYDGEIVYQSKRI
ERHKEVANLLVEKGRAYHCYCPEDEVAEKKIKAREEGKIYKHKCTTKPPSCHPSSRHWDPEENMWSRAGMTSGVRSVIRF
KVPDSQEIVIDDKIYGQIKVNSNQLDDIVILRSDNTPTYIFAVVIDDHDAGITDIIRGSDHLTNTFKHLLIYQALDFDIP
RFAHVPLIHGEDGNKLSKRHGATSVCDYEKMGILPKAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKK
LEHLNNHYISNMSNEDILTLMLRENTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQDPPLDLSEEAEQIVKSNLD
IIKLLASFLSEIGDENWNKGFLSSQIKECAKLHDMKISDVYHSLRAPITGVMDAPGIIDIMVILGKDECIRRLQAI
>Mature_476_residues
MLDVVTRFAPSPTGFLHIGGARTALFNWLYAKHHGGRFLLRIEDTDRKRSTKEAIDAIIEGLRWLGVSYDGEIVYQSKRI
ERHKEVANLLVEKGRAYHCYCPEDEVAEKKIKAREEGKIYKHKCTTKPPSCHPSSRHWDPEENMWSRAGMTSGVRSVIRF
KVPDSQEIVIDDKIYGQIKVNSNQLDDIVILRSDNTPTYIFAVVIDDHDAGITDIIRGSDHLTNTFKHLLIYQALDFDIP
RFAHVPLIHGEDGNKLSKRHGATSVCDYEKMGILPKAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKK
LEHLNNHYISNMSNEDILTLMLRENTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQDPPLDLSEEAEQIVKSNLD
IIKLLASFLSEIGDENWNKGFLSSQIKECAKLHDMKISDVYHSLRAPITGVMDAPGIIDIMVILGKDECIRRLQAI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=502, Percent_Identity=29.8804780876494, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI62241042, Length=246, Percent_Identity=26.0162601626016, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788743, Length=478, Percent_Identity=37.4476987447699, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI226510925, Length=299, Percent_Identity=30.7692307692308, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786895, Length=219, Percent_Identity=28.310502283105, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=506, Percent_Identity=31.8181818181818, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=31.1377245508982, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=247, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=103, Percent_Identity=35.9223300970874, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24665321, Length=500, Percent_Identity=32.6, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI17864488, Length=103, Percent_Identity=37.8640776699029, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54409; Mature: 54409

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDVVTRFAPSPTGFLHIGGARTALFNWLYAKHHGGRFLLRIEDTDRKRSTKEAIDAIIE
CCCCHHHCCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHH
GLRWLGVSYDGEIVYQSKRIERHKEVANLLVEKGRAYHCYCPEDEVAEKKIKAREEGKIY
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEE
KHKCTTKPPSCHPSSRHWDPEENMWSRAGMTSGVRSVIRFKVPDSQEIVIDDKIYGQIKV
EEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHEEECCCCCEEEEECCEEEEEEE
NSNQLDDIVILRSDNTPTYIFAVVIDDHDAGITDIIRGSDHLTNTFKHLLIYQALDFDIP
CCCCCCEEEEEECCCCCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
RFAHVPLIHGEDGNKLSKRHGATSVCDYEKMGILPKAMRNYLLRLGWSHGNDEIISDEQA
CCCCCCEEECCCCCHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
IEWFNLESIGRSPARLDFKKLEHLNNHYISNMSNEDILTLMLRENTLTDKKKGYLLQGLT
HHEECHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
ELKKRANYLTELLDLAKFYIQDPPLDLSEEAEQIVKSNLDIIKLLASFLSEIGDENWNKG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCH
FLSSQIKECAKLHDMKISDVYHSLRAPITGVMDAPGIIDIMVILGKDECIRRLQAI
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHCC
>Mature Secondary Structure
MLDVVTRFAPSPTGFLHIGGARTALFNWLYAKHHGGRFLLRIEDTDRKRSTKEAIDAIIE
CCCCHHHCCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHH
GLRWLGVSYDGEIVYQSKRIERHKEVANLLVEKGRAYHCYCPEDEVAEKKIKAREEGKIY
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEE
KHKCTTKPPSCHPSSRHWDPEENMWSRAGMTSGVRSVIRFKVPDSQEIVIDDKIYGQIKV
EEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHEEECCCCCEEEEECCEEEEEEE
NSNQLDDIVILRSDNTPTYIFAVVIDDHDAGITDIIRGSDHLTNTFKHLLIYQALDFDIP
CCCCCCEEEEEECCCCCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
RFAHVPLIHGEDGNKLSKRHGATSVCDYEKMGILPKAMRNYLLRLGWSHGNDEIISDEQA
CCCCCCEEECCCCCHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
IEWFNLESIGRSPARLDFKKLEHLNNHYISNMSNEDILTLMLRENTLTDKKKGYLLQGLT
HHEECHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
ELKKRANYLTELLDLAKFYIQDPPLDLSEEAEQIVKSNLDIIKLLASFLSEIGDENWNKG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCH
FLSSQIKECAKLHDMKISDVYHSLRAPITGVMDAPGIIDIMVILGKDECIRRLQAI
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA