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The map label for this gene is ksgA [H]

Identifier: 225630006

GI number: 225630006

Start: 161171

End: 161989

Strand: Direct

Name: ksgA [H]

Synonym: WRi_001610

Alternate gene names: 225630006

Gene position: 161171-161989 (Clockwise)

Preceding gene: 225630004

Following gene: 225630007

Centisome position: 11.15

GC content: 33.09

Gene sequence:

>819_bases
ATGAAAAAATTTTTACTGAAGCCAAAAAAGAGTCTAGGGCAAAATTTTATTTTATCGAGTGAGATAACAAAAAAAATCGT
CGCTTTAGCTGGTAGTCTGGAAAATTTTAATGTCATTGAAATTGGTCCTGGATATGGTGCATTAACAAGAGAAATATTGG
TGCATAATCCAAAGTCTCTACTTTCTATAGAAAAAGATAGAGATTTAGTAAAACACCATGACCAACTGCTAAATGAGCAT
CAAGGAAAGTATAGAATTATAGAAGCGGATGCACTGCATGTTACAGAAGAAGAGCTGATAGAGCGCCCAGTTAAAGTTAT
TGCTAACTTGCCTTACAATATCTCGGTAGTGTTATTTTTAAAGTGGTTAAATAATATAAAATTTTTTACGAACTTGACAT
TAATGTTTCAAAAAGAGGTAGCAGATCGCATTACGGCAAGACCCAATTCTAAAGATTATGGTTCCTTATCAGTGCTAAGC
CAGTTGCTATGTGATATAAAAAAGGAATTTGATATTGAACCTAAAGAGTTTTTTCCAAGACCAAAAATACATTCTTCAGT
AATTACAGTAAATCCTTTGCCTATCCCAAAATTTGCAGTAAATTTGGAAACCTTAACAAGATTAACACGTGCTGTTTTTT
CTCAAAGAAGAAAGATGCTGAGAAATAGCTTGCAAAATATAACGAACCACACTGAAACTGTCCTTGAAAACGCTAAATTG
AGTGGAAACGAACGTCCAGAAAACTTAACTATTGAGCAGTTTTGTTTGCTGGCAAATAATGTAGAATGCTTATTTTGCAA
AAGCCCTATTGCAATTTAA

Upstream 100 bases:

>100_bases
CTACCAATTGAGCTAAGCCGGCGTTAAACACATTAATAATATACAATTATCAATGAATTTTAGTCAACTAATTTTTAAAA
TACTGTATAATGAAAATAAT

Downstream 100 bases:

>100_bases
TTAATTGGCGTATAAGCTTTAAATAAAGCCTATGAAAAAAATGGAAGCTTGTTATTCGTTTGACGATATACTTCTTTTGC
CAGCCTATTCTAATATATTG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKKFLLKPKKSLGQNFILSSEITKKIVALAGSLENFNVIEIGPGYGALTREILVHNPKSLLSIEKDRDLVKHHDQLLNEH
QGKYRIIEADALHVTEEELIERPVKVIANLPYNISVVLFLKWLNNIKFFTNLTLMFQKEVADRITARPNSKDYGSLSVLS
QLLCDIKKEFDIEPKEFFPRPKIHSSVITVNPLPIPKFAVNLETLTRLTRAVFSQRRKMLRNSLQNITNHTETVLENAKL
SGNERPENLTIEQFCLLANNVECLFCKSPIAI

Sequences:

>Translated_272_residues
MKKFLLKPKKSLGQNFILSSEITKKIVALAGSLENFNVIEIGPGYGALTREILVHNPKSLLSIEKDRDLVKHHDQLLNEH
QGKYRIIEADALHVTEEELIERPVKVIANLPYNISVVLFLKWLNNIKFFTNLTLMFQKEVADRITARPNSKDYGSLSVLS
QLLCDIKKEFDIEPKEFFPRPKIHSSVITVNPLPIPKFAVNLETLTRLTRAVFSQRRKMLRNSLQNITNHTETVLENAKL
SGNERPENLTIEQFCLLANNVECLFCKSPIAI
>Mature_272_residues
MKKFLLKPKKSLGQNFILSSEITKKIVALAGSLENFNVIEIGPGYGALTREILVHNPKSLLSIEKDRDLVKHHDQLLNEH
QGKYRIIEADALHVTEEELIERPVKVIANLPYNISVVLFLKWLNNIKFFTNLTLMFQKEVADRITARPNSKDYGSLSVLS
QLLCDIKKEFDIEPKEFFPRPKIHSSVITVNPLPIPKFAVNLETLTRLTRAVFSQRRKMLRNSLQNITNHTETVLENAKL
SGNERPENLTIEQFCLLANNVECLFCKSPIAI

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=284, Percent_Identity=34.8591549295775, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI7657198, Length=221, Percent_Identity=30.316742081448, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786236, Length=258, Percent_Identity=35.2713178294574, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25141369, Length=293, Percent_Identity=28.6689419795222, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25146882, Length=229, Percent_Identity=30.5676855895196, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=226, Percent_Identity=28.3185840707965, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21357273, Length=281, Percent_Identity=32.3843416370107, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21358017, Length=242, Percent_Identity=30.5785123966942, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31084; Mature: 31084

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLLKPKKSLGQNFILSSEITKKIVALAGSLENFNVIEIGPGYGALTREILVHNPKSL
CCCCCCCCHHHCCCCCEEHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCHHH
LSIEKDRDLVKHHDQLLNEHQGKYRIIEADALHVTEEELIERPVKVIANLPYNISVVLFL
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
KWLNNIKFFTNLTLMFQKEVADRITARPNSKDYGSLSVLSQLLCDIKKEFDIEPKEFFPR
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCC
PKIHSSVITVNPLPIPKFAVNLETLTRLTRAVFSQRRKMLRNSLQNITNHTETVLENAKL
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGNERPENLTIEQFCLLANNVECLFCKSPIAI
CCCCCCCCCCHHHHHHHHCCCEEEEECCCCCC
>Mature Secondary Structure
MKKFLLKPKKSLGQNFILSSEITKKIVALAGSLENFNVIEIGPGYGALTREILVHNPKSL
CCCCCCCCHHHCCCCCEEHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCHHH
LSIEKDRDLVKHHDQLLNEHQGKYRIIEADALHVTEEELIERPVKVIANLPYNISVVLFL
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
KWLNNIKFFTNLTLMFQKEVADRITARPNSKDYGSLSVLSQLLCDIKKEFDIEPKEFFPR
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCC
PKIHSSVITVNPLPIPKFAVNLETLTRLTRAVFSQRRKMLRNSLQNITNHTETVLENAKL
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGNERPENLTIEQFCLLANNVECLFCKSPIAI
CCCCCCCCCCHHHHHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA