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The map label for this gene is pepA [H]

Identifier: 225629910

GI number: 225629910

Start: 50030

End: 51568

Strand: Direct

Name: pepA [H]

Synonym: WRi_000430

Alternate gene names: 225629910

Gene position: 50030-51568 (Clockwise)

Preceding gene: 225629909

Following gene: 225629911

Centisome position: 3.46

GC content: 36.78

Gene sequence:

>1539_bases
ATGAAAAATATCTTACTGATGTTTTTGCTATGTTTTATGTACAGTTTACAATTATTTGCAACGGAGATACCAGCAATGAA
AATAACAATTTCTAAAATTTCGCCCGATTTTAAAACAATAGTAATGGGTTTATTTGAAGACAACGAAACCGTAAATGATG
ACGGAGTTTTGCAAGGAAAACAGGTCATAGATAATATAAAGCAATTTAGCGATTTCAATGGAAGTTTTGGTGAATTTTTC
TCTACTGCTTTGCCAGAAGAAAAAAATGTTATAGTTGTTGGACTGGGTAAGAAGGATGAATGGAATGAAAATAAAGAATT
AAATATTGGTGGTAAAATATATTGTGAGCTAAGCAGATTAAAAATTAAAAAAGCAGCGGTTTTAATCGAAGGTAGTGCAG
CAAATGTTGCATATGGTGCGTTTCTGCGTAGTTTTAAGTTTGATAAGTATAAAACTAAAAAGGATGAGAAAATTACAGAG
GTAGAGGAGATTACCGTATTAGTAAAAGATGAACAATTAAGTAATGCTGAAAGATCATTTGAGCACTTAAGGCAAGAAGG
TGAGAGTATATTCCTTGCGCGCTCTTTTATAACAGAGCCTCCTAACATTCTATATCCAGAATCCTATGCTGATCATATAA
AAAAAGAACTTACTAAGCTTGGCCTTGAAATCGAGGTGCTTGATAAAAAGCAGATGGAAGAGAAAAAAATGGGAGCCTTG
CTTGGAGTTGCACAAGGAAGTAGTAAAGAACCAAAATTAGTAGTGATAAAATGGAATGGGGCTTCTAAAGAACAAAAGCC
TATAGCTTTTGTTGGTAAAGGTATAACGTTTGACACTGGTGGAGTATCACTCAAACCTTCACGTGGTATGGAGTCGATGA
AATATGATATGGCAGGCTCTGCTACTGTGGTTGGGGTGATGCATGCTTTAGCAGGACGAAAAGCAAAAGTAAATGCAATT
GGCGTGGTCGCACTTGCAGAAAATGCAGTGGGTGGTAATGCTCAAAGACCAAGTGATGTAGTAACTTCAATGTCTGGACA
AACAATAGAAGTGTTGAACACCGATGCAGAAGGAAGGCTCATACTTGCAGATGCTTTATGGTATACGCAAGATAGATTCT
CACCAAAATTTATGATTGATCTTGCAACTTTAACTGGTGCTATAGTGGTTGCACTTGGAAATAACGAATATGCTGGTCTT
TTTTCAAATAATGATGAATTAGCAAACCGTCTGATTGATGCAGGAAATGAAGTAAATGAGAAGTTATGGCGTTTTCCTAT
GAATGAAACTTATGACAAAATTATTGATTCACCGATTGCTGATGTTCAAAACATCGCTCCTGCAGGCTCTGGTGGAGATA
GCATAATGGCTGCACAGTTTTTACAGCGTTTTGTGAATGAAACTTGCTGGGCACATTTAGATATCGCAGGCACTGCTTGG
CACGAGAAAGGTACTGACATTTGTCCAAGAGGAGCAGTAGGTTTTGGTGTAAGGTTACTTAATAAGTTGGTTGAGAAGTA
CTACGAAGCCAATGATTAA

Upstream 100 bases:

>100_bases
TACCTTGGGCGGATATAATAAGTTCTCTAATAAAAGATAAAAAACCTTGCATGTAAGGGAGTCCATAGTAAACTTAAGTG
TTATTGTACTTAGGATGTAT

Downstream 100 bases:

>100_bases
AGGTCTTTATACTGGATTTATGGCTTAGCTACATGAGCGTTTATTTTCGAGGCGCAGCAAATGAAAGTAGCTGATACATA
TTTCTTTTTTTCTGGATTCC

Product: leucyl aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKNILLMFLLCFMYSLQLFATEIPAMKITISKISPDFKTIVMGLFEDNETVNDDGVLQGKQVIDNIKQFSDFNGSFGEFF
STALPEEKNVIVVGLGKKDEWNENKELNIGGKIYCELSRLKIKKAAVLIEGSAANVAYGAFLRSFKFDKYKTKKDEKITE
VEEITVLVKDEQLSNAERSFEHLRQEGESIFLARSFITEPPNILYPESYADHIKKELTKLGLEIEVLDKKQMEEKKMGAL
LGVAQGSSKEPKLVVIKWNGASKEQKPIAFVGKGITFDTGGVSLKPSRGMESMKYDMAGSATVVGVMHALAGRKAKVNAI
GVVALAENAVGGNAQRPSDVVTSMSGQTIEVLNTDAEGRLILADALWYTQDRFSPKFMIDLATLTGAIVVALGNNEYAGL
FSNNDELANRLIDAGNEVNEKLWRFPMNETYDKIIDSPIADVQNIAPAGSGGDSIMAAQFLQRFVNETCWAHLDIAGTAW
HEKGTDICPRGAVGFGVRLLNKLVEKYYEAND

Sequences:

>Translated_512_residues
MKNILLMFLLCFMYSLQLFATEIPAMKITISKISPDFKTIVMGLFEDNETVNDDGVLQGKQVIDNIKQFSDFNGSFGEFF
STALPEEKNVIVVGLGKKDEWNENKELNIGGKIYCELSRLKIKKAAVLIEGSAANVAYGAFLRSFKFDKYKTKKDEKITE
VEEITVLVKDEQLSNAERSFEHLRQEGESIFLARSFITEPPNILYPESYADHIKKELTKLGLEIEVLDKKQMEEKKMGAL
LGVAQGSSKEPKLVVIKWNGASKEQKPIAFVGKGITFDTGGVSLKPSRGMESMKYDMAGSATVVGVMHALAGRKAKVNAI
GVVALAENAVGGNAQRPSDVVTSMSGQTIEVLNTDAEGRLILADALWYTQDRFSPKFMIDLATLTGAIVVALGNNEYAGL
FSNNDELANRLIDAGNEVNEKLWRFPMNETYDKIIDSPIADVQNIAPAGSGGDSIMAAQFLQRFVNETCWAHLDIAGTAW
HEKGTDICPRGAVGFGVRLLNKLVEKYYEAND
>Mature_512_residues
MKNILLMFLLCFMYSLQLFATEIPAMKITISKISPDFKTIVMGLFEDNETVNDDGVLQGKQVIDNIKQFSDFNGSFGEFF
STALPEEKNVIVVGLGKKDEWNENKELNIGGKIYCELSRLKIKKAAVLIEGSAANVAYGAFLRSFKFDKYKTKKDEKITE
VEEITVLVKDEQLSNAERSFEHLRQEGESIFLARSFITEPPNILYPESYADHIKKELTKLGLEIEVLDKKQMEEKKMGAL
LGVAQGSSKEPKLVVIKWNGASKEQKPIAFVGKGITFDTGGVSLKPSRGMESMKYDMAGSATVVGVMHALAGRKAKVNAI
GVVALAENAVGGNAQRPSDVVTSMSGQTIEVLNTDAEGRLILADALWYTQDRFSPKFMIDLATLTGAIVVALGNNEYAGL
FSNNDELANRLIDAGNEVNEKLWRFPMNETYDKIIDSPIADVQNIAPAGSGGDSIMAAQFLQRFVNETCWAHLDIAGTAW
HEKGTDICPRGAVGFGVRLLNKLVEKYYEAND

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=439, Percent_Identity=36.2186788154898, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI47155554, Length=379, Percent_Identity=30.3430079155673, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1790710, Length=440, Percent_Identity=39.0909090909091, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI87082123, Length=318, Percent_Identity=34.5911949685535, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17556903, Length=376, Percent_Identity=30.8510638297872, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17565172, Length=234, Percent_Identity=29.0598290598291, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355725, Length=471, Percent_Identity=31.4225053078556, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24661038, Length=490, Percent_Identity=31.0204081632653, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24662227, Length=471, Percent_Identity=30.1486199575372, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI20129969, Length=369, Percent_Identity=30.0813008130081, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI161077148, Length=478, Percent_Identity=28.2426778242678, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI20130057, Length=478, Percent_Identity=28.2426778242678, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19922386, Length=448, Percent_Identity=29.2410714285714, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI21355645, Length=465, Percent_Identity=27.0967741935484, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24662223, Length=465, Percent_Identity=27.0967741935484, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI20129963, Length=422, Percent_Identity=27.9620853080569, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI221379063, Length=345, Percent_Identity=32.7536231884058, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI221379062, Length=345, Percent_Identity=32.7536231884058, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI21357381, Length=342, Percent_Identity=32.7485380116959, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24646701, Length=282, Percent_Identity=24.822695035461, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24646703, Length=282, Percent_Identity=24.822695035461, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21358201, Length=282, Percent_Identity=24.822695035461, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283 [H]

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 56448; Mature: 56448

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILLMFLLCFMYSLQLFATEIPAMKITISKISPDFKTIVMGLFEDNETVNDDGVLQGK
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHEEHCCCCCCCCCCCCCCHH
QVIDNIKQFSDFNGSFGEFFSTALPEEKNVIVVGLGKKDEWNENKELNIGGKIYCELSRL
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEEEHH
KIKKAAVLIEGSAANVAYGAFLRSFKFDKYKTKKDEKITEVEEITVLVKDEQLSNAERSF
EEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEECCHHHHHHHHH
EHLRQEGESIFLARSFITEPPNILYPESYADHIKKELTKLGLEIEVLDKKQMEEKKMGAL
HHHHHCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHH
LGVAQGSSKEPKLVVIKWNGASKEQKPIAFVGKGITFDTGGVSLKPSRGMESMKYDMAGS
EEECCCCCCCCEEEEEEECCCCCCCCCEEEEECCEEECCCCEEECCCCCCHHHHHCCCCC
ATVVGVMHALAGRKAKVNAIGVVALAENAVGGNAQRPSDVVTSMSGQTIEVLNTDAEGRL
HHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCE
ILADALWYTQDRFSPKFMIDLATLTGAIVVALGNNEYAGLFSNNDELANRLIDAGNEVNE
EEEEHHHHHHCCCCCEEEEEHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCHHHH
KLWRFPMNETYDKIIDSPIADVQNIAPAGSGGDSIMAAQFLQRFVNETCWAHLDIAGTAW
HHHCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCC
HEKGTDICPRGAVGFGVRLLNKLVEKYYEAND
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNILLMFLLCFMYSLQLFATEIPAMKITISKISPDFKTIVMGLFEDNETVNDDGVLQGK
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHEEHCCCCCCCCCCCCCCHH
QVIDNIKQFSDFNGSFGEFFSTALPEEKNVIVVGLGKKDEWNENKELNIGGKIYCELSRL
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEEEHH
KIKKAAVLIEGSAANVAYGAFLRSFKFDKYKTKKDEKITEVEEITVLVKDEQLSNAERSF
EEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEECCHHHHHHHHH
EHLRQEGESIFLARSFITEPPNILYPESYADHIKKELTKLGLEIEVLDKKQMEEKKMGAL
HHHHHCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHH
LGVAQGSSKEPKLVVIKWNGASKEQKPIAFVGKGITFDTGGVSLKPSRGMESMKYDMAGS
EEECCCCCCCCEEEEEEECCCCCCCCCEEEEECCEEECCCCEEECCCCCCHHHHHCCCCC
ATVVGVMHALAGRKAKVNAIGVVALAENAVGGNAQRPSDVVTSMSGQTIEVLNTDAEGRL
HHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCE
ILADALWYTQDRFSPKFMIDLATLTGAIVVALGNNEYAGLFSNNDELANRLIDAGNEVNE
EEEEHHHHHHCCCCCEEEEEHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCHHHH
KLWRFPMNETYDKIIDSPIADVQNIAPAGSGGDSIMAAQFLQRFVNETCWAHLDIAGTAW
HHHCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCC
HEKGTDICPRGAVGFGVRLLNKLVEKYYEAND
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA