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The map label for this gene is virB10 [H]

Identifier: 225629878

GI number: 225629878

Start: 5784

End: 7280

Strand: Direct

Name: virB10 [H]

Synonym: WRi_000070

Alternate gene names: 225629878

Gene position: 5784-7280 (Clockwise)

Preceding gene: 225629877

Following gene: 225629879

Centisome position: 0.4

GC content: 36.34

Gene sequence:

>1497_bases
ATGAATAAAGAAAGGCGTAACAATTCAGAAGATGAATCAGAAATAGAGAATAAGGTAGTAACAGTTGGCTCTAATCAAGG
TCATAGGGCATTGATGGTCATGATTTTAGTGCTTCTGGTTGGTGGAGTGTATTATTTCTATTTTAGTCCTTCTCATAAAG
AGGATTCAGAAGTTATTAAAAAAGAGGAAACAAAGCAAAACGTTCAAGAATTTAAAGGAAAGTTGGAACAAGTTCCAGAT
AATGTAATAGTTCCAGAAAGAATAATAACCGATCCCTTACCACCCTTACCTCCTCTTCCTACACCACAAATCGTACCAGA
AATAAAACAAATTAAAAAAGAAGAAGAGAAACCAAAAGAAACCCCTGTGTCAAATATACCTATTTTGCCAAAGCAAAATT
TTCCTTCTAACAATGTTATTAGTAATCTACCTACCTCGTTTCCTACAATAGGGGATGGTGGTTACCCTAGAGAAAGACGT
AGTGCACAAATGTTAACAATTTCAGGTGGTAGTGGAGAGAACAAGGCTGCTGATGCTATTTTATCCGACACTTCAGTACA
GTCGAGCAAAGCTACCAGAGTAGGAAAGCTTGGTTTAATGATTACTCAAGGTAAAATTATTGATGCTGTTCTTGAAACTG
CAATAAACTCTGATCTGCAAGGAATGCTGCGTGCTATGGTGAGTAGAGATGTTTATGCAGAAACTGGTGATACAGTTTTA
ATACCTAAAGGCTCAAGATTGATAGGCAGTTATTCATTTGATTCGAATGTTGCTAAATCTCGTGTAAATATAAACTGGAA
CAGAGTGATTCTGCCACATGGAATAGATATTGCAATCTCATCACTTAGTACTGACGAGCTTGGTAGAGCAGGGATAGCGG
GAATAGTTGATAATAAAATAGTAAGTGCGTTATTCTCTTCAGTGGCACTTGCTGGTGTTTCAATTAGTTCGGCTGTTATA
GGGCAAAAGGCTTCTAATCTTATCGATGTGCTAACTGCAATGGATGTAGTGAAGTCTATTACTGCAACTGAAATAGATAT
TTCTTCTCTCAAAGGGGCTATTGTTGAGAGATTGGAGGATGCTATAAAAACTGAAGTTGGGAAGGTAGGAGACGAAAAAT
GGAAATTGGGTCTTAGGGCTATTGAAAAAATTCGAAATGCTAAAAGTGATCAGGATTTACTGAGAATAATAAAGGAAGAA
ATAGTAAGTGTGATAAAAAGTTCTGGAGCTAGTGGAGTTGATGCAATTACTAGTGACAATATCGGTATAACTTTAGACGA
TGTACGTCAACTGTTGAGGCAAACTCAGAAAAGAAGCAAATCTGTTTATGATGAAGCAATCGGGAAATCAATCAATGATT
TTTCTAAAGACATGCGAGATATAGTGGGCAGATATACAGATAAAAAACCAACTATTTATGTCGATCAAGGCACTGCATTG
AAAGTATTTGTTAACCAAGATATAGTATTTCCTCCACAGGCAATATTAAATCAATGA

Upstream 100 bases:

>100_bases
TATGTTATAATAAAGGGAGTACATAAAAAGCTATTTATGCGTTATGAAGATGGTGAAGTTGAAATTATAAATAGGTCACT
TTAGCTGTGGTACATGCAAT

Downstream 100 bases:

>100_bases
AATGAACTATGCTGCACTTGATACATATTTGGAGCCATTACAAGGCATATTTCAAGAAGAAGGCGTAAATGAAATATCAA
TAAATAAGCCAAAGGAAGTA

Product: type IV secretion system protein VirB10

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNKERRNNSEDESEIENKVVTVGSNQGHRALMVMILVLLVGGVYYFYFSPSHKEDSEVIKKEETKQNVQEFKGKLEQVPD
NVIVPERIITDPLPPLPPLPTPQIVPEIKQIKKEEEKPKETPVSNIPILPKQNFPSNNVISNLPTSFPTIGDGGYPRERR
SAQMLTISGGSGENKAADAILSDTSVQSSKATRVGKLGLMITQGKIIDAVLETAINSDLQGMLRAMVSRDVYAETGDTVL
IPKGSRLIGSYSFDSNVAKSRVNINWNRVILPHGIDIAISSLSTDELGRAGIAGIVDNKIVSALFSSVALAGVSISSAVI
GQKASNLIDVLTAMDVVKSITATEIDISSLKGAIVERLEDAIKTEVGKVGDEKWKLGLRAIEKIRNAKSDQDLLRIIKEE
IVSVIKSSGASGVDAITSDNIGITLDDVRQLLRQTQKRSKSVYDEAIGKSINDFSKDMRDIVGRYTDKKPTIYVDQGTAL
KVFVNQDIVFPPQAILNQ

Sequences:

>Translated_498_residues
MNKERRNNSEDESEIENKVVTVGSNQGHRALMVMILVLLVGGVYYFYFSPSHKEDSEVIKKEETKQNVQEFKGKLEQVPD
NVIVPERIITDPLPPLPPLPTPQIVPEIKQIKKEEEKPKETPVSNIPILPKQNFPSNNVISNLPTSFPTIGDGGYPRERR
SAQMLTISGGSGENKAADAILSDTSVQSSKATRVGKLGLMITQGKIIDAVLETAINSDLQGMLRAMVSRDVYAETGDTVL
IPKGSRLIGSYSFDSNVAKSRVNINWNRVILPHGIDIAISSLSTDELGRAGIAGIVDNKIVSALFSSVALAGVSISSAVI
GQKASNLIDVLTAMDVVKSITATEIDISSLKGAIVERLEDAIKTEVGKVGDEKWKLGLRAIEKIRNAKSDQDLLRIIKEE
IVSVIKSSGASGVDAITSDNIGITLDDVRQLLRQTQKRSKSVYDEAIGKSINDFSKDMRDIVGRYTDKKPTIYVDQGTAL
KVFVNQDIVFPPQAILNQ
>Mature_498_residues
MNKERRNNSEDESEIENKVVTVGSNQGHRALMVMILVLLVGGVYYFYFSPSHKEDSEVIKKEETKQNVQEFKGKLEQVPD
NVIVPERIITDPLPPLPPLPTPQIVPEIKQIKKEEEKPKETPVSNIPILPKQNFPSNNVISNLPTSFPTIGDGGYPRERR
SAQMLTISGGSGENKAADAILSDTSVQSSKATRVGKLGLMITQGKIIDAVLETAINSDLQGMLRAMVSRDVYAETGDTVL
IPKGSRLIGSYSFDSNVAKSRVNINWNRVILPHGIDIAISSLSTDELGRAGIAGIVDNKIVSALFSSVALAGVSISSAVI
GQKASNLIDVLTAMDVVKSITATEIDISSLKGAIVERLEDAIKTEVGKVGDEKWKLGLRAIEKIRNAKSDQDLLRIIKEE
IVSVIKSSGASGVDAITSDNIGITLDDVRQLLRQTQKRSKSVYDEAIGKSINDFSKDMRDIVGRYTDKKPTIYVDQGTAL
KVFVNQDIVFPPQAILNQ

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG2948

COG function: function code U; Type IV secretory pathway, VirB10 components

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbI/virB10 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005498 [H]

Pfam domain/function: PF03743 TrbI [H]

EC number: NA

Molecular weight: Translated: 54286; Mature: 54286

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKERRNNSEDESEIENKVVTVGSNQGHRALMVMILVLLVGGVYYFYFSPSHKEDSEVIK
CCCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
KEETKQNVQEFKGKLEQVPDNVIVPERIITDPLPPLPPLPTPQIVPEIKQIKKEEEKPKE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TPVSNIPILPKQNFPSNNVISNLPTSFPTIGDGGYPRERRSAQMLTISGGSGENKAADAI
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LSDTSVQSSKATRVGKLGLMITQGKIIDAVLETAINSDLQGMLRAMVSRDVYAETGDTVL
HCCCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHCCCCEEE
IPKGSRLIGSYSFDSNVAKSRVNINWNRVILPHGIDIAISSLSTDELGRAGIAGIVDNKI
EECCCEEEEECCCCCCHHHHEECCCCCEEEECCCCEEEEECCCHHHHCCCCHHHHHHHHH
VSALFSSVALAGVSISSAVIGQKASNLIDVLTAMDVVKSITATEIDISSLKGAIVERLED
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AIKTEVGKVGDEKWKLGLRAIEKIRNAKSDQDLLRIIKEEIVSVIKSSGASGVDAITSDN
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IGITLDDVRQLLRQTQKRSKSVYDEAIGKSINDFSKDMRDIVGRYTDKKPTIYVDQGTAL
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEE
KVFVNQDIVFPPQAILNQ
EEEECCCCCCCCHHHHCC
>Mature Secondary Structure
MNKERRNNSEDESEIENKVVTVGSNQGHRALMVMILVLLVGGVYYFYFSPSHKEDSEVIK
CCCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
KEETKQNVQEFKGKLEQVPDNVIVPERIITDPLPPLPPLPTPQIVPEIKQIKKEEEKPKE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TPVSNIPILPKQNFPSNNVISNLPTSFPTIGDGGYPRERRSAQMLTISGGSGENKAADAI
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LSDTSVQSSKATRVGKLGLMITQGKIIDAVLETAINSDLQGMLRAMVSRDVYAETGDTVL
HCCCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHCCCCEEE
IPKGSRLIGSYSFDSNVAKSRVNINWNRVILPHGIDIAISSLSTDELGRAGIAGIVDNKI
EECCCEEEEECCCCCCHHHHEECCCCCEEEECCCCEEEEECCCHHHHCCCCHHHHHHHHH
VSALFSSVALAGVSISSAVIGQKASNLIDVLTAMDVVKSITATEIDISSLKGAIVERLED
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AIKTEVGKVGDEKWKLGLRAIEKIRNAKSDQDLLRIIKEEIVSVIKSSGASGVDAITSDN
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IGITLDDVRQLLRQTQKRSKSVYDEAIGKSINDFSKDMRDIVGRYTDKKPTIYVDQGTAL
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEE
KVFVNQDIVFPPQAILNQ
EEEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10882236 [H]