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The map label for this gene is menD

Identifier: 222527160

GI number: 222527160

Start: 4893734

End: 4895497

Strand: Direct

Name: menD

Synonym: Chy400_3942

Alternate gene names: 222527160

Gene position: 4893734-4895497 (Clockwise)

Preceding gene: 222527159

Following gene: 222527161

Centisome position: 92.88

GC content: 60.66

Gene sequence:

>1764_bases
ATGAACGTTACAGCGCATATCGAAGCGCTGACGCATTGGGTGGCAGCAATTGCGCAGAGTCTGGCGGCAAATGGGGTCAA
AGATGTGGTTGTTTGCCCCGGTTCGCGTAGTACACCACTGGCGCTGGCCGTAGCGCGCCACCCGTCGTTGCGGGTCTGGA
TGCATCTGGATGAGCGTTCAGCCGGTTTTTTCGCACTGGGCATGGCTCGTGCGCGCGGGATGCCGGCTGCTGTGCTGTGT
ACCTCCGGCACGGCGGTGGCCAATCTGTTGCCGGCAGTCGTTGAAGCTAACCTCGCCCGGGTGCCGCTCGTGATCCTGAC
TGCCGACCGCCCACCTGAGTTGCGCGATAATGGTGCGCCGCAAACAATTGATCAGATCGGAATTTTCGGGCGCAATGTGC
GCTGGTTTGTTGATCTGCCCACACCGGATATGGCACTCTTGCCCTTTTTACGGGCTACGCTGGGTCGTGCTGTCGGACTG
GCGCGCAGTGCTCCTGCCGGCCCGGTGCACCTGAATCTGCCCTTTCGAGAACCACTCGTTCCTGATCGGGCGCGGATGGC
AGCCCTGTTTAGCGAAACGCCATCAGCGGTTCAGGTAACGCCGGCACGTCGCATGATCGGTGGAGCAGAACTGGCAATGC
TGGCAACCAGCCTGGTTGAATACCGCCGGGGACTGATCATCGCCGGTCCCGATTGTCCACCCGATCTTGGCCCACTGCTC
ACCACCCTGGCCCATCGTCTGCGCTACCCCATCCTGGCCGATCCATTGTCGGGAGTACGCTACGGGCCGCACGTCGATGA
GTATACCCTGGGCACATATGATGCCTTCTTGCGTGATGAGCGCTTCGTGTCCAGTTACGCGCCAGAAGTTGTCTTGCGCT
TCGGGGCAATGCCAACCAGCAAACCGCTCTTGCTTTACCTGCAACACCATCCTCACGCTCGCCAGATTGTGATTGATGGC
GGTGCCGGCTGGCGTGAGCCGACCAGCCTGGCCCGCGAACACCTGCACGTCGATGAACACTGGTTCTGTATCGCGCTGGC
CGACGCGCTGGCATCCTCGCAACGTGAAGGGCCGACCCTCTGGCTACGCGCATGGGTAAGCGCCGAGCAGACGGCACGGA
CGATCATTCAGTCACATCTCCTACCCCAAGAGCACATCAGTGAACCGGGACTGTTTGCCCGTCTCGGTTCCTGGTTGCCT
GCCGGTGCAACGCTGTTTGTTGGCAATTCGATGCCGGTACGCGATTGCGACACGTTTCTCGGCCCACGCACGAACCCGCT
GTACGTGGTTGGCAATCGGGGAGCTAACGGCATTGATGGCCTGGTTTCAACCGCGCTCGGCCTGGCTGCCGGTCAGGCGA
GACCGCTCGTGATGGCGTTGGGTGACCTTTCGCTCCTGCACGACTCCAACGGCCTGCTTGCCGCTCGGCTCCACCGGCTC
GATGCTACGATCCTGTTGATCAACAACGATGGTGGCGGTATCTTCTCGTTTCTGCCCCAGGCCAGCGAAACTGACCAGTT
CGAGCTATTATTCGGTACGCCACACGGTGTTGATTTCGCACCACTGGCGGCAATGCACAACGCGCACTACACCCTGGCCA
CAGATTGGTCAGCGGTACACGAAGCACTTCAGGCCAGTTTTACCGGCGGCTTGCATCTGATCGAGGTACGCACCCGGCGC
GATCAAAATGTCATTGATCACCGCACGATCTGGCCACTGGTCAGTAATGCGCTGGCCCAGGCCGGGATTACGGTCGCCGA
ATAG

Upstream 100 bases:

>100_bases
CTGATCCAGAGCGTGAGCTAACCGAAACCAGAATTAAGCTGCGGGCAATGTTAGAGGCGCTTGGGGCAAGTGAAGAGATG
ATTGGCTAAGCGAGGGTTGC

Downstream 100 bases:

>100_bases
CATGCCTCATAGCTTCAATGAGTCACACAAGGGTAAGGATGTCGTGATGATCAATACGCTGAGCACAAATGCCGAGATTA
TTGCGCAAGAAGCGCTCTAC

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNVTAHIEALTHWVAAIAQSLAANGVKDVVVCPGSRSTPLALAVARHPSLRVWMHLDERSAGFFALGMARARGMPAAVLC
TSGTAVANLLPAVVEANLARVPLVILTADRPPELRDNGAPQTIDQIGIFGRNVRWFVDLPTPDMALLPFLRATLGRAVGL
ARSAPAGPVHLNLPFREPLVPDRARMAALFSETPSAVQVTPARRMIGGAELAMLATSLVEYRRGLIIAGPDCPPDLGPLL
TTLAHRLRYPILADPLSGVRYGPHVDEYTLGTYDAFLRDERFVSSYAPEVVLRFGAMPTSKPLLLYLQHHPHARQIVIDG
GAGWREPTSLAREHLHVDEHWFCIALADALASSQREGPTLWLRAWVSAEQTARTIIQSHLLPQEHISEPGLFARLGSWLP
AGATLFVGNSMPVRDCDTFLGPRTNPLYVVGNRGANGIDGLVSTALGLAAGQARPLVMALGDLSLLHDSNGLLAARLHRL
DATILLINNDGGGIFSFLPQASETDQFELLFGTPHGVDFAPLAAMHNAHYTLATDWSAVHEALQASFTGGLHLIEVRTRR
DQNVIDHRTIWPLVSNALAQAGITVAE

Sequences:

>Translated_587_residues
MNVTAHIEALTHWVAAIAQSLAANGVKDVVVCPGSRSTPLALAVARHPSLRVWMHLDERSAGFFALGMARARGMPAAVLC
TSGTAVANLLPAVVEANLARVPLVILTADRPPELRDNGAPQTIDQIGIFGRNVRWFVDLPTPDMALLPFLRATLGRAVGL
ARSAPAGPVHLNLPFREPLVPDRARMAALFSETPSAVQVTPARRMIGGAELAMLATSLVEYRRGLIIAGPDCPPDLGPLL
TTLAHRLRYPILADPLSGVRYGPHVDEYTLGTYDAFLRDERFVSSYAPEVVLRFGAMPTSKPLLLYLQHHPHARQIVIDG
GAGWREPTSLAREHLHVDEHWFCIALADALASSQREGPTLWLRAWVSAEQTARTIIQSHLLPQEHISEPGLFARLGSWLP
AGATLFVGNSMPVRDCDTFLGPRTNPLYVVGNRGANGIDGLVSTALGLAAGQARPLVMALGDLSLLHDSNGLLAARLHRL
DATILLINNDGGGIFSFLPQASETDQFELLFGTPHGVDFAPLAAMHNAHYTLATDWSAVHEALQASFTGGLHLIEVRTRR
DQNVIDHRTIWPLVSNALAQAGITVAE
>Mature_587_residues
MNVTAHIEALTHWVAAIAQSLAANGVKDVVVCPGSRSTPLALAVARHPSLRVWMHLDERSAGFFALGMARARGMPAAVLC
TSGTAVANLLPAVVEANLARVPLVILTADRPPELRDNGAPQTIDQIGIFGRNVRWFVDLPTPDMALLPFLRATLGRAVGL
ARSAPAGPVHLNLPFREPLVPDRARMAALFSETPSAVQVTPARRMIGGAELAMLATSLVEYRRGLIIAGPDCPPDLGPLL
TTLAHRLRYPILADPLSGVRYGPHVDEYTLGTYDAFLRDERFVSSYAPEVVLRFGAMPTSKPLLLYLQHHPHARQIVIDG
GAGWREPTSLAREHLHVDEHWFCIALADALASSQREGPTLWLRAWVSAEQTARTIIQSHLLPQEHISEPGLFARLGSWLP
AGATLFVGNSMPVRDCDTFLGPRTNPLYVVGNRGANGIDGLVSTALGLAAGQARPLVMALGDLSLLHDSNGLLAARLHRL
DATILLINNDGGGIFSFLPQASETDQFELLFGTPHGVDFAPLAAMHNAHYTLATDWSAVHEALQASFTGGLHLIEVRTRR
DQNVIDHRTIWPLVSNALAQAGITVAE

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=543, Percent_Identity=32.4125230202578, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1790104, Length=164, Percent_Identity=29.8780487804878, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_CHLAA (A9WB45)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001637227.1
- ProteinModelPortal:   A9WB45
- GeneID:   5825462
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_3655
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK2477406
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 63290; Mature: 63290

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTAHIEALTHWVAAIAQSLAANGVKDVVVCPGSRSTPLALAVARHPSLRVWMHLDERS
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCEEEEEEECCCC
AGFFALGMARARGMPAAVLCTSGTAVANLLPAVVEANLARVPLVILTADRPPELRDNGAP
CCEEEEHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCC
QTIDQIGIFGRNVRWFVDLPTPDMALLPFLRATLGRAVGLARSAPAGPVHLNLPFREPLV
CHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
PDRARMAALFSETPSAVQVTPARRMIGGAELAMLATSLVEYRRGLIIAGPDCPPDLGPLL
CCHHHHHHHHHCCCCCEEECHHHHHHCHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHH
TTLAHRLRYPILADPLSGVRYGPHVDEYTLGTYDAFLRDERFVSSYAPEVVLRFGAMPTS
HHHHHHHCCCCEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCHHHHHHHCCCCCC
KPLLLYLQHHPHARQIVIDGGAGWREPTSLAREHLHVDEHWFCIALADALASSQREGPTL
CCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCEE
WLRAWVSAEQTARTIIQSHLLPQEHISEPGLFARLGSWLPAGATLFVGNSMPVRDCDTFL
EEEEHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHC
GPRTNPLYVVGNRGANGIDGLVSTALGLAAGQARPLVMALGDLSLLHDSNGLLAARLHRL
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHHC
DATILLINNDGGGIFSFLPQASETDQFELLFGTPHGVDFAPLAAMHNAHYTLATDWSAVH
CEEEEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCHHHHH
EALQASFTGGLHLIEVRTRRDQNVIDHRTIWPLVSNALAQAGITVAE
HHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MNVTAHIEALTHWVAAIAQSLAANGVKDVVVCPGSRSTPLALAVARHPSLRVWMHLDERS
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCEEEEEEECCCC
AGFFALGMARARGMPAAVLCTSGTAVANLLPAVVEANLARVPLVILTADRPPELRDNGAP
CCEEEEHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCC
QTIDQIGIFGRNVRWFVDLPTPDMALLPFLRATLGRAVGLARSAPAGPVHLNLPFREPLV
CHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
PDRARMAALFSETPSAVQVTPARRMIGGAELAMLATSLVEYRRGLIIAGPDCPPDLGPLL
CCHHHHHHHHHCCCCCEEECHHHHHHCHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHH
TTLAHRLRYPILADPLSGVRYGPHVDEYTLGTYDAFLRDERFVSSYAPEVVLRFGAMPTS
HHHHHHHCCCCEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCHHHHHHHCCCCCC
KPLLLYLQHHPHARQIVIDGGAGWREPTSLAREHLHVDEHWFCIALADALASSQREGPTL
CCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCEE
WLRAWVSAEQTARTIIQSHLLPQEHISEPGLFARLGSWLPAGATLFVGNSMPVRDCDTFL
EEEEHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHC
GPRTNPLYVVGNRGANGIDGLVSTALGLAAGQARPLVMALGDLSLLHDSNGLLAARLHRL
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHHC
DATILLINNDGGGIFSFLPQASETDQFELLFGTPHGVDFAPLAAMHNAHYTLATDWSAVH
CEEEEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCHHHHH
EALQASFTGGLHLIEVRTRRDQNVIDHRTIWPLVSNALAQAGITVAE
HHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA