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The map label for this gene is menF [H]

Identifier: 222527159

GI number: 222527159

Start: 4892274

End: 4893722

Strand: Direct

Name: menF [H]

Synonym: Chy400_3941

Alternate gene names: 222527159

Gene position: 4892274-4893722 (Clockwise)

Preceding gene: 222527158

Following gene: 222527160

Centisome position: 92.85

GC content: 60.11

Gene sequence:

>1449_bases
ATGACCAATCCTCTCATCGGAGTACGGGTAACTACTGCCACGCTACGCGAAGTGCCGCGGATGGAGGCGGCTGCCGATGT
GGCCCGGCAACGCGCTCGCGAGCTTGGGCGACCGGTGTTGGTGAGTATCACCACTCGTGTCGGGCTGCGCGATCCCCTGG
CGCTGTTTGCCCGTGGTGCCGGTGTTACCCATAATCGCTTTTTCTGGAGCACGCCGGCCGCTGATTTTGAATTGACCGGT
CTCGGTGCAGCATGGAGTTTTACACCAACATCTGGCGCTGATCGCTTTGCTGTCAGTGCTGCGGCATGGAAGCAGTTGGT
GAGTGAGGCAGTGATTGATGCCGATCCCGAACTGCCGGTACAGGGACCCGTTGCCGTGGCCGGGTTTCGTTTTGATCCCG
ATCAACCCCCAAGCGATTTGTGGCGGGATTATCCGATGGCATTGCTGATGGTACCACGCTTGCTGATCGTGCGCCACGGT
GATGTGACAACGTTGACGGTGAACGGGATGGTGGATGCGCACAGTTCGACGCTGGCACTAGGGGCAGCCGCTGCCCGTCG
TTTGCAGGCACTGATCGGTCTCCCACTACGACCGCGGCGTCCTCCGCTTGATTTGCAGGTTGATGTCTCGCTCGATCCCG
AGACCTGGAAAGCTATTGTTGCCAATGCCGTGAGCGAACTCCGCGCCGGTACGTTGGAGAAGGTGGTGCTGGCACGTGCT
GTGCGTTTGCGCAGCACAGAACCGTTTGATACGGCTGCGACGCTGCACGGGTTGCGCCGGGATTATCCGCATACATTTGT
CTTTGCCGTGGCGCGCGGTGGCCGAACATTTCTCGGCGCTTCACCCGAACGTCTGGTAGCGCTCCGGAATGGCGCAGTGG
CCGCTTCGGCGCTCGCCGGTACGGCACCACGTGGCAAGACACCGGATGAGGATGCCGCGTTGGCCGCGGCGTTGTTGCAA
AGCGCTAAAGATCGCGCCGAGCACGCTTTTGTGGTGCAGATGATTCGCTCTGCGCTGGCCGAGTATTGCGACGCGGTGCA
GGCGCCGTCTACCCCTGAGATTATGCGGGTGCGTAATGTCCAGCATCTGTTTACACCGATCACAGCCTGCATCCGAGCCG
GGTACGATATTTTTGATCTGGTTGGGCGTCTTCACCCAACTCCGGCGGTTGGTGGTAAACCGGGTCCCGCTGCACTGGCC
TGGATTCGTGATCACGAACGACTTGATCGGGGATGGTACGCGGCTCCCGTGGGATGGGTTGATGCCCGGGGTGATGGCGA
GTTCGCTGTTGCCCTCCGTTCGGCATTGATCGGGCGGCGCGAAGCAACGCTTTTTGCCGGTTGTGGGATTGTGGCAGCTT
CTGATCCAGAGCGTGAGCTAACCGAAACCAGAATTAAGCTGCGGGCAATGTTAGAGGCGCTTGGGGCAAGTGAAGAGATG
ATTGGCTAA

Upstream 100 bases:

>100_bases
GTTATCTCGCATCAGAACAACCATAACGTGATACAATGCATAAAGGCAAGATTGTAACAATCCCGGGTCGTAAAGGGAGC
GTGTACTACATCACAATCGT

Downstream 100 bases:

>100_bases
GCGAGGGTTGCATGAACGTTACAGCGCATATCGAAGCGCTGACGCATTGGGTGGCAGCAATTGCGCAGAGTCTGGCGGCA
AATGGGGTCAAAGATGTGGT

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTNPLIGVRVTTATLREVPRMEAAADVARQRARELGRPVLVSITTRVGLRDPLALFARGAGVTHNRFFWSTPAADFELTG
LGAAWSFTPTSGADRFAVSAAAWKQLVSEAVIDADPELPVQGPVAVAGFRFDPDQPPSDLWRDYPMALLMVPRLLIVRHG
DVTTLTVNGMVDAHSSTLALGAAAARRLQALIGLPLRPRRPPLDLQVDVSLDPETWKAIVANAVSELRAGTLEKVVLARA
VRLRSTEPFDTAATLHGLRRDYPHTFVFAVARGGRTFLGASPERLVALRNGAVAASALAGTAPRGKTPDEDAALAAALLQ
SAKDRAEHAFVVQMIRSALAEYCDAVQAPSTPEIMRVRNVQHLFTPITACIRAGYDIFDLVGRLHPTPAVGGKPGPAALA
WIRDHERLDRGWYAAPVGWVDARGDGEFAVALRSALIGRREATLFAGCGIVAASDPERELTETRIKLRAMLEALGASEEM
IG

Sequences:

>Translated_482_residues
MTNPLIGVRVTTATLREVPRMEAAADVARQRARELGRPVLVSITTRVGLRDPLALFARGAGVTHNRFFWSTPAADFELTG
LGAAWSFTPTSGADRFAVSAAAWKQLVSEAVIDADPELPVQGPVAVAGFRFDPDQPPSDLWRDYPMALLMVPRLLIVRHG
DVTTLTVNGMVDAHSSTLALGAAAARRLQALIGLPLRPRRPPLDLQVDVSLDPETWKAIVANAVSELRAGTLEKVVLARA
VRLRSTEPFDTAATLHGLRRDYPHTFVFAVARGGRTFLGASPERLVALRNGAVAASALAGTAPRGKTPDEDAALAAALLQ
SAKDRAEHAFVVQMIRSALAEYCDAVQAPSTPEIMRVRNVQHLFTPITACIRAGYDIFDLVGRLHPTPAVGGKPGPAALA
WIRDHERLDRGWYAAPVGWVDARGDGEFAVALRSALIGRREATLFAGCGIVAASDPERELTETRIKLRAMLEALGASEEM
IG
>Mature_481_residues
TNPLIGVRVTTATLREVPRMEAAADVARQRARELGRPVLVSITTRVGLRDPLALFARGAGVTHNRFFWSTPAADFELTGL
GAAWSFTPTSGADRFAVSAAAWKQLVSEAVIDADPELPVQGPVAVAGFRFDPDQPPSDLWRDYPMALLMVPRLLIVRHGD
VTTLTVNGMVDAHSSTLALGAAAARRLQALIGLPLRPRRPPLDLQVDVSLDPETWKAIVANAVSELRAGTLEKVVLARAV
RLRSTEPFDTAATLHGLRRDYPHTFVFAVARGGRTFLGASPERLVALRNGAVAASALAGTAPRGKTPDEDAALAAALLQS
AKDRAEHAFVVQMIRSALAEYCDAVQAPSTPEIMRVRNVQHLFTPITACIRAGYDIFDLVGRLHPTPAVGGKPGPAALAW
IRDHERLDRGWYAAPVGWVDARGDGEFAVALRSALIGRREATLFAGCGIVAASDPERELTETRIKLRAMLEALGASEEMI
G

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=417, Percent_Identity=31.1750599520384, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI87082077, Length=424, Percent_Identity=31.1320754716981, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787518, Length=370, Percent_Identity=25.9459459459459, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788114, Length=200, Percent_Identity=30, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=367, Percent_Identity=28.3378746594005, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51741; Mature: 51610

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPLIGVRVTTATLREVPRMEAAADVARQRARELGRPVLVSITTRVGLRDPLALFARGA
CCCCCEEEEEEHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCHHHHHHCCC
GVTHNRFFWSTPAADFELTGLGAAWSFTPTSGADRFAVSAAAWKQLVSEAVIDADPELPV
CCCCCCEEEECCCCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
QGPVAVAGFRFDPDQPPSDLWRDYPMALLMVPRLLIVRHGDVTTLTVNGMVDAHSSTLAL
CCCEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHEECCCEEEEEECCEEECCCCHHHH
GAAAARRLQALIGLPLRPRRPPLDLQVDVSLDPETWKAIVANAVSELRAGTLEKVVLARA
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRLRSTEPFDTAATLHGLRRDYPHTFVFAVARGGRTFLGASPERLVALRNGAVAASALAG
HHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCHHHEEEECCCCHHHHHHHC
TAPRGKTPDEDAALAAALLQSAKDRAEHAFVVQMIRSALAEYCDAVQAPSTPEIMRVRNV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QHLFTPITACIRAGYDIFDLVGRLHPTPAVGGKPGPAALAWIRDHERLDRGWYAAPVGWV
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCE
DARGDGEFAVALRSALIGRREATLFAGCGIVAASDPERELTETRIKLRAMLEALGASEEM
ECCCCCHHHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHC
IG
CC
>Mature Secondary Structure 
TNPLIGVRVTTATLREVPRMEAAADVARQRARELGRPVLVSITTRVGLRDPLALFARGA
CCCCEEEEEEHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCHHHHHHCCC
GVTHNRFFWSTPAADFELTGLGAAWSFTPTSGADRFAVSAAAWKQLVSEAVIDADPELPV
CCCCCCEEEECCCCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
QGPVAVAGFRFDPDQPPSDLWRDYPMALLMVPRLLIVRHGDVTTLTVNGMVDAHSSTLAL
CCCEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHEECCCEEEEEECCEEECCCCHHHH
GAAAARRLQALIGLPLRPRRPPLDLQVDVSLDPETWKAIVANAVSELRAGTLEKVVLARA
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRLRSTEPFDTAATLHGLRRDYPHTFVFAVARGGRTFLGASPERLVALRNGAVAASALAG
HHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCHHHEEEECCCCHHHHHHHC
TAPRGKTPDEDAALAAALLQSAKDRAEHAFVVQMIRSALAEYCDAVQAPSTPEIMRVRNV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QHLFTPITACIRAGYDIFDLVGRLHPTPAVGGKPGPAALAWIRDHERLDRGWYAAPVGWV
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCE
DARGDGEFAVALRSALIGRREATLFAGCGIVAASDPERELTETRIKLRAMLEALGASEEM
ECCCCCHHHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHC
IG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]