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The map label for this gene is argG [H]

Identifier: 222527044

GI number: 222527044

Start: 4764556

End: 4765905

Strand: Reverse

Name: argG [H]

Synonym: Chy400_3823

Alternate gene names: 222527044

Gene position: 4765905-4764556 (Counterclockwise)

Preceding gene: 222527049

Following gene: 222527040

Centisome position: 90.45

GC content: 56.44

Gene sequence:

>1350_bases
ATGGCTAAAATTCTGCAACACCTGCCGGTTGGCGAAAAGATCGGGATCGCTTTTTCCGGCGGCCTGGACACCAGTGCTGC
AATCCACTGGATGCGCGCCAAAGGTGCTATTCCCTATGCATACACCGCCAATCTCGGTCAGCCCGATGAACCCGATTATG
AGGATATTCCCCGCCGGGCACTGATGTACGGCGCCGAAGCAGCCCGCCTGATCGATTGCCGCCAGCAGTTGGTCGCTGAG
GGACTGGCTGCCCTACAGTGCGGTGCATTTCACATCTCAACCGCCGGTTTGCCCTACTTTAATACCACTCCACTCGGACG
TGCAGTAACCGGTACCATGCTGGTCGCGGCCATGCGCGAAGATGGGGTTCATATCTGGGGGGACGGCAGTACCTACAAAG
GGAACGATATCGAACGGTTTTATCGCTATGGCTTGCTGGTCAACCCCGATCTGCGTATCTATAAACCGTGGCTGGATCAG
GCGTTTATCGACGAATTGGGTGGTCGGGCCGAGATGTCTGCCTACCTGAGTCGGGCCGGTTTTGAGTATCGCATGCCGGC
GGAAAAAGCCTATTCAACCGACTCCAATATGCTGGGTGCCACTCACGAAGCGAAAGACCTGGAGTTTCTCGACCGCAACA
TCACGATTGTCAAGCCAATTATGGGCGTGGCCTTCTGGCAGCCGGATGTGCATATCGAATACGAAGAGGTACGCATCACC
TTTGAAGAGGGCTTCCCGGTTGCGATTAATGGCATGACCTTCCCCGACCACGTCGCCCTCATGGCCGAAGCCAACCGCAT
TGGTGGACGACACGGCCTCGGTATGAGTGATCAAATCGAGAATCGGATTATCGAAGCCAAGAGTCGGGGTATCTACGAAG
CCCCTGGCATGGCGCTGCTCTACATTGCCTACGAGCGGCTGGTAACCGGCATTCACAACGAAGATACGATTGATCAATAC
CGTGAGTACGGGCGAAAACTGGGGCGTCTCCTCTACGCCGGGCGCTGGTTCGATCCACAGGCGATGATGATCCGTGAGTC
GTTGCAGCGTTGGGTTGCCCGCCTGATCACCGGTGAGGTGACGATTGGTCTGCGGCGTGGTAACGATTATTCAATCCTGG
ACACGACCTCACCTAATTTAACCTACGACCCGGCCCGGCTGACGATGGAGAAGGGGGAAGCCAGCTTCACACCCCAGGAT
CGGATTGGGCAGTTGCACATGCGCAACAACGACATTGCCGATACGCGCAAGAAGCTCATGGTTTACCTGCGCACCGGTCT
CCTCGGCGAGAGTGGCGTATTGCCGCAACTTGCTCCGCCGGATGATGAGGATGTAGGAGAGAGGAAATAG

Upstream 100 bases:

>100_bases
GACTCGCAGATTGGCGAATTGAGCGATCCTGCGCTGTTCGTCACTACTCATCGTATAATACAACGGCAAAAGACACCCTT
CCTTACTGAAGAGGAGTGCT

Downstream 100 bases:

>100_bases
GGTTTAGGAGATAGGAAATAGGAACTAGGATTTAGGAGATAGGAGATAGTGAGGTAGGATTTAGGAGGTAGGAGATAGAA
GGAGATAGGAAATAGGAGGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAKILQHLPVGEKIGIAFSGGLDTSAAIHWMRAKGAIPYAYTANLGQPDEPDYEDIPRRALMYGAEAARLIDCRQQLVAE
GLAALQCGAFHISTAGLPYFNTTPLGRAVTGTMLVAAMREDGVHIWGDGSTYKGNDIERFYRYGLLVNPDLRIYKPWLDQ
AFIDELGGRAEMSAYLSRAGFEYRMPAEKAYSTDSNMLGATHEAKDLEFLDRNITIVKPIMGVAFWQPDVHIEYEEVRIT
FEEGFPVAINGMTFPDHVALMAEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLYIAYERLVTGIHNEDTIDQY
REYGRKLGRLLYAGRWFDPQAMMIRESLQRWVARLITGEVTIGLRRGNDYSILDTTSPNLTYDPARLTMEKGEASFTPQD
RIGQLHMRNNDIADTRKKLMVYLRTGLLGESGVLPQLAPPDDEDVGERK

Sequences:

>Translated_449_residues
MAKILQHLPVGEKIGIAFSGGLDTSAAIHWMRAKGAIPYAYTANLGQPDEPDYEDIPRRALMYGAEAARLIDCRQQLVAE
GLAALQCGAFHISTAGLPYFNTTPLGRAVTGTMLVAAMREDGVHIWGDGSTYKGNDIERFYRYGLLVNPDLRIYKPWLDQ
AFIDELGGRAEMSAYLSRAGFEYRMPAEKAYSTDSNMLGATHEAKDLEFLDRNITIVKPIMGVAFWQPDVHIEYEEVRIT
FEEGFPVAINGMTFPDHVALMAEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLYIAYERLVTGIHNEDTIDQY
REYGRKLGRLLYAGRWFDPQAMMIRESLQRWVARLITGEVTIGLRRGNDYSILDTTSPNLTYDPARLTMEKGEASFTPQD
RIGQLHMRNNDIADTRKKLMVYLRTGLLGESGVLPQLAPPDDEDVGERK
>Mature_448_residues
AKILQHLPVGEKIGIAFSGGLDTSAAIHWMRAKGAIPYAYTANLGQPDEPDYEDIPRRALMYGAEAARLIDCRQQLVAEG
LAALQCGAFHISTAGLPYFNTTPLGRAVTGTMLVAAMREDGVHIWGDGSTYKGNDIERFYRYGLLVNPDLRIYKPWLDQA
FIDELGGRAEMSAYLSRAGFEYRMPAEKAYSTDSNMLGATHEAKDLEFLDRNITIVKPIMGVAFWQPDVHIEYEEVRITF
EEGFPVAINGMTFPDHVALMAEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLYIAYERLVTGIHNEDTIDQYR
EYGRKLGRLLYAGRWFDPQAMMIRESLQRWVARLITGEVTIGLRRGNDYSILDTTSPNLTYDPARLTMEKGEASFTPQDR
IGQLHMRNNDIADTRKKLMVYLRTGLLGESGVLPQLAPPDDEDVGERK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=405, Percent_Identity=27.4074074074074, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI16950633, Length=405, Percent_Identity=27.4074074074074, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1789563, Length=439, Percent_Identity=75.1708428246014, Blast_Score=693, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=409, Percent_Identity=28.6063569682152, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21358151, Length=398, Percent_Identity=27.8894472361809, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 50308; Mature: 50177

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILQHLPVGEKIGIAFSGGLDTSAAIHWMRAKGAIPYAYTANLGQPDEPDYEDIPRRA
CCCHHHHCCCCCEEEEEEECCCCCHHHEEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
LMYGAEAARLIDCRQQLVAEGLAALQCGAFHISTAGLPYFNTTPLGRAVTGTMLVAAMRE
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
DGVHIWGDGSTYKGNDIERFYRYGLLVNPDLRIYKPWLDQAFIDELGGRAEMSAYLSRAG
CCEEEEECCCCCCCCHHHHHHHCCCEECCCCEEECHHHHHHHHHHHCCHHHHHHHHHHCC
FEYRMPAEKAYSTDSNMLGATHEAKDLEFLDRNITIVKPIMGVAFWQPDVHIEYEEVRIT
CCEECCHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHEEECCCCEEEEEEEEEE
FEEGFPVAINGMTFPDHVALMAEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
EECCCCEEECCCCCCHHHHHEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEH
YIAYERLVTGIHNEDTIDQYREYGRKLGRLLYAGRWFDPQAMMIRESLQRWVARLITGEV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCE
TIGLRRGNDYSILDTTSPNLTYDPARLTMEKGEASFTPQDRIGQLHMRNNDIADTRKKLM
EEEEEECCCEEEEECCCCCCEECHHHEEEECCCCCCCCHHHCCEEEECCCCHHHHHHHHH
VYLRTGLLGESGVLPQLAPPDDEDVGERK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKILQHLPVGEKIGIAFSGGLDTSAAIHWMRAKGAIPYAYTANLGQPDEPDYEDIPRRA
CCHHHHCCCCCEEEEEEECCCCCHHHEEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
LMYGAEAARLIDCRQQLVAEGLAALQCGAFHISTAGLPYFNTTPLGRAVTGTMLVAAMRE
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
DGVHIWGDGSTYKGNDIERFYRYGLLVNPDLRIYKPWLDQAFIDELGGRAEMSAYLSRAG
CCEEEEECCCCCCCCHHHHHHHCCCEECCCCEEECHHHHHHHHHHHCCHHHHHHHHHHCC
FEYRMPAEKAYSTDSNMLGATHEAKDLEFLDRNITIVKPIMGVAFWQPDVHIEYEEVRIT
CCEECCHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHEEECCCCEEEEEEEEEE
FEEGFPVAINGMTFPDHVALMAEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
EECCCCEEECCCCCCHHHHHEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEH
YIAYERLVTGIHNEDTIDQYREYGRKLGRLLYAGRWFDPQAMMIRESLQRWVARLITGEV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCE
TIGLRRGNDYSILDTTSPNLTYDPARLTMEKGEASFTPQDRIGQLHMRNNDIADTRKKLM
EEEEEECCCEEEEECCCCCCEECHHHEEEECCCCCCCCHHHCCEEEECCCCHHHHHHHHH
VYLRTGLLGESGVLPQLAPPDDEDVGERK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA