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The map label for this gene is rffG [H]

Identifier: 222525739

GI number: 222525739

Start: 3132898

End: 3133911

Strand: Reverse

Name: rffG [H]

Synonym: Chy400_2490

Alternate gene names: 222525739

Gene position: 3133911-3132898 (Counterclockwise)

Preceding gene: 222525740

Following gene: 222525736

Centisome position: 59.48

GC content: 55.33

Gene sequence:

>1014_bases
ATGCGGAATCTGCTCGTTACCGGCGGAGCCGGCTTCATTGGCAGTAACTTTGTCCACTATATGCTTGGCAAATATGATGA
TTACCGGATTGTGGTGTACGACAAGCTGACGTATGCCGGTAATCTGGCTAACCTGGCCCCGGTTGCCAATGATCCACGCT
TTGCCTTTGTGCAGGGCGATATTTGTGATATTGATGCAGTACGGCAGGCTGTACGTTCGTACAACATCGACACCATCGTC
AACTTTGCCGCCGAAACGCACGTTGATCGGTCGATCATGGCCCCTGATGCCGTCGTGCGCACGAACGTCAACGGCACCTG
GGCCTTACTCGAAGTGGCCCGCGAGTTTCAGCTTGAGCGATTTCACCAGATCAGTACTGACGAGGTGTACGGCGCTATTC
CGGCACCGCGCCGGTCACGCGAGGGTGATCCGCTCGAACCGCGCAGCCCCTATTCGGCCAGCAAAGCCGGTGCCGAGCAT
CTGGTGTACGCCTACTACATCACCTATGGCGTACCAATGACGATTACACGCGGCTCGAACAATATTGGGCCGTACCACTA
CCCGGAAAAGGCGGTACCACTCTTCACCACCAACGCGATTGACAATCTGCCCCTGCCGATCTATGGCGATGGCCTGCAAG
TGCGCGATTATCAATACGTCCTCGACCATTGCGAAGCGATTGATCTTGTGCTGCACAAGGGTCAGATTGGGGAAGTATAC
AATGTGGGTACCGAGGTCGAAACGCCCAATATCGAAATGGCCCGCAAGATTCTCGATATTCTCGGCAAGCCCTACAGTCT
TATTCAACACGTCACCGACCGGGCCGGTCATGATCGGCGCTATGCGCTCGATTGCTCCAAATTACGCGCGTTAGGCTGGC
GTTCACGCCATACCTTCGACGAAGCACTTGAAAAGACGGTGCGCTGGTTCGTTGAGAACGAAGCCTGGTGGCGACCGATT
AAATCGGGGGAGTATCTGGAATACTACCGCCGCCAGTATGTCGAACGGGGGTAG

Upstream 100 bases:

>100_bases
GCTGCGCATCCGGTATGCTATAACCCAATTGCGGGTATACCGAACGCTCGCAGGATGTTGCAGGTCATCATTCTGGTATA
TGAAAGGAACAGGCCGTCCT

Downstream 100 bases:

>100_bases
CATACCGGCGGTGAGGTTACAGCCAATCCCAGGGGCGACCGATTGGCCGCCCCTGTATTATTAAGCAGATTTGCATCAAA
CCTGGAATCCGTCAGCCGAT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MRNLLVTGGAGFIGSNFVHYMLGKYDDYRIVVYDKLTYAGNLANLAPVANDPRFAFVQGDICDIDAVRQAVRSYNIDTIV
NFAAETHVDRSIMAPDAVVRTNVNGTWALLEVAREFQLERFHQISTDEVYGAIPAPRRSREGDPLEPRSPYSASKAGAEH
LVYAYYITYGVPMTITRGSNNIGPYHYPEKAVPLFTTNAIDNLPLPIYGDGLQVRDYQYVLDHCEAIDLVLHKGQIGEVY
NVGTEVETPNIEMARKILDILGKPYSLIQHVTDRAGHDRRYALDCSKLRALGWRSRHTFDEALEKTVRWFVENEAWWRPI
KSGEYLEYYRRQYVERG

Sequences:

>Translated_337_residues
MRNLLVTGGAGFIGSNFVHYMLGKYDDYRIVVYDKLTYAGNLANLAPVANDPRFAFVQGDICDIDAVRQAVRSYNIDTIV
NFAAETHVDRSIMAPDAVVRTNVNGTWALLEVAREFQLERFHQISTDEVYGAIPAPRRSREGDPLEPRSPYSASKAGAEH
LVYAYYITYGVPMTITRGSNNIGPYHYPEKAVPLFTTNAIDNLPLPIYGDGLQVRDYQYVLDHCEAIDLVLHKGQIGEVY
NVGTEVETPNIEMARKILDILGKPYSLIQHVTDRAGHDRRYALDCSKLRALGWRSRHTFDEALEKTVRWFVENEAWWRPI
KSGEYLEYYRRQYVERG
>Mature_337_residues
MRNLLVTGGAGFIGSNFVHYMLGKYDDYRIVVYDKLTYAGNLANLAPVANDPRFAFVQGDICDIDAVRQAVRSYNIDTIV
NFAAETHVDRSIMAPDAVVRTNVNGTWALLEVAREFQLERFHQISTDEVYGAIPAPRRSREGDPLEPRSPYSASKAGAEH
LVYAYYITYGVPMTITRGSNNIGPYHYPEKAVPLFTTNAIDNLPLPIYGDGLQVRDYQYVLDHCEAIDLVLHKGQIGEVY
NVGTEVETPNIEMARKILDILGKPYSLIQHVTDRAGHDRRYALDCSKLRALGWRSRHTFDEALEKTVRWFVENEAWWRPI
KSGEYLEYYRRQYVERG

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=37.8048780487805, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI42516563, Length=316, Percent_Identity=26.5822784810127, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=26.0744985673352, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=46.685878962536, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=44.5682451253482, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1786974, Length=334, Percent_Identity=27.5449101796407, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788366, Length=172, Percent_Identity=30.8139534883721, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=323, Percent_Identity=35.6037151702786, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=318, Percent_Identity=33.3333333333333, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71982035, Length=344, Percent_Identity=27.0348837209302, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=316, Percent_Identity=25.6329113924051, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=265, Percent_Identity=29.0566037735849, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356223, Length=316, Percent_Identity=26.5822784810127, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19923002, Length=347, Percent_Identity=27.6657060518732, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38422; Mature: 38422

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLLVTGGAGFIGSNFVHYMLGKYDDYRIVVYDKLTYAGNLANLAPVANDPRFAFVQGD
CCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECEECCCCCCCCCCCCCCCCEEEEECC
ICDIDAVRQAVRSYNIDTIVNFAAETHVDRSIMAPDAVVRTNVNGTWALLEVAREFQLER
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCEEEECCCCHHHHHHHHHHHHHHH
FHQISTDEVYGAIPAPRRSREGDPLEPRSPYSASKAGAEHLVYAYYITYGVPMTITRGSN
HHCCCCHHHEECCCCCCCCCCCCCCCCCCCCCCHHCCHHHEEEEEEEECCCEEEEECCCC
NIGPYHYPEKAVPLFTTNAIDNLPLPIYGDGLQVRDYQYVLDHCEAIDLVLHKGQIGEVY
CCCCCCCCCCCCCEEECCCCCCCCCCEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEE
NVGTEVETPNIEMARKILDILGKPYSLIQHVTDRAGHDRRYALDCSKLRALGWRSRHTFD
ECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCCCHH
EALEKTVRWFVENEAWWRPIKSGEYLEYYRRQYVERG
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNLLVTGGAGFIGSNFVHYMLGKYDDYRIVVYDKLTYAGNLANLAPVANDPRFAFVQGD
CCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECEECCCCCCCCCCCCCCCCEEEEECC
ICDIDAVRQAVRSYNIDTIVNFAAETHVDRSIMAPDAVVRTNVNGTWALLEVAREFQLER
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCEEEECCCCHHHHHHHHHHHHHHH
FHQISTDEVYGAIPAPRRSREGDPLEPRSPYSASKAGAEHLVYAYYITYGVPMTITRGSN
HHCCCCHHHEECCCCCCCCCCCCCCCCCCCCCCHHCCHHHEEEEEEEECCCEEEEECCCC
NIGPYHYPEKAVPLFTTNAIDNLPLPIYGDGLQVRDYQYVLDHCEAIDLVLHKGQIGEVY
CCCCCCCCCCCCCEEECCCCCCCCCCEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEE
NVGTEVETPNIEMARKILDILGKPYSLIQHVTDRAGHDRRYALDCSKLRALGWRSRHTFD
ECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCCCHH
EALEKTVRWFVENEAWWRPIKSGEYLEYYRRQYVERG
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]